Acute reduction of Sertoli cell numbers during development leads to a subsequent reduction in sperm numbers in adulthood

2014 ◽  
Author(s):  
Annalucia L Darbey ◽  
Peter O'Shaughnessy ◽  
Jean-Luc Pitetti ◽  
Serge Nef ◽  
Lee Smith ◽  
...  
Keyword(s):  
Sertoli Cell ◽  
Subsequent Reduction ◽  
Cell Numbers

Aromatase and 11β-hydroxysteroid dehydrogenase 2 localisation in the testes of pigs from birth to puberty linked to changes of hormone pattern and testicular morphology

2008 ◽  
Vol 20 (4) ◽  
pp. 505 ◽  
Author(s):  
A. Wagner ◽  
R. Claus
Keyword(s):  
Blood Plasma ◽  
Sertoli Cell ◽  
Germ Cells ◽  
Leydig Cells ◽  
Hydroxysteroid Dehydrogenase ◽  
Blood Collection ◽  
Testicular Development ◽  
Post Mortem ◽  
Cell Numbers ◽  
Testicular Morphology

Oestrogens and glucocorticoids are important for spermatogenesis and are regulated via aromatase for oestradiol synthesis and 11β-hydroxysteroid dehydrogenase 2 (11β-HSD 2) as an inactivator of cortisol. In the present study postnatal changes of these two enzymes were monitored together with testicular development and hormone concentrations. Pigs were assigned to three periods: Weeks 0–5, Weeks 5–11 or Weeks 11–17. In Period 1, groups of four piglets were killed after each week. Blood plasma and testes were sampled immediately post mortem. For Periods 2 and 3, groups of six pigs were fitted with vein catheters for daily blood collection. Testes from all pigs were obtained after killing. Levels of testosterone, oestradiol, LH, FSH and cortisol were determined radioimmunologically. The 11β-HSD 2- and aromatase-expressing cells were stained immunocytochemically. All hormones were maximal 2 weeks after birth. A rise of LH, testosterone and oestradiol occurred again at Week 17. FSH and cortisol remained basal. Parallel to the first postnatal rise, the presence of aromatase and 11β-HSD 2 in Leydig cells increased, together with germ and Sertoli cell numbers. Expression was low from 3 to 5 weeks, was resumed after Week 5 and was maximal at Week 17. The amount of 11β-HSD 2 in germ cells was greatest at birth, decreased thereafter and was absent after Week 3.

scholarly journals Ram lambs need FSH for normal testicular growth, Sertoli cell numbers and onset of spermatogenesis

1998 ◽  
Vol 38 (5) ◽  
pp. 539-550 ◽  
Author(s):  
Robert J. Kilgour ◽  
Claudine Pisselet ◽  
Maurice P. Dubois ◽  
Michel Courot
Keyword(s):  
Sertoli Cell ◽  
Cell Numbers ◽  
Testicular Growth ◽  
Ram Lambs

scholarly journals Stimulation of Sertoli cell proliferation: defining the response interval to an inhibitor of estrogen synthesis in the boar

Reproduction ◽  
2012 ◽  
Vol 143 (4) ◽  
pp. 523-529 ◽  
Author(s):  
Trish Berger ◽  
Lisa Kentfield ◽  
J F Roser ◽  
Alan Conley
Keyword(s):  
Cell Proliferation ◽  
Sertoli Cell ◽  
Cell Number ◽  
Cell Numbers ◽  
Response Interval ◽  
Estrogen Synthesis ◽  
Letrozole Treatment ◽  
Size At Sexual Maturity ◽  
Testicular Size ◽  
Second Wave

Sertoli cell proliferation occurs in two major waves after birth, one neonatally and another prepubertally, each contributing to final testicular size and sperm production. However, little is known about the regulation of either wave. We have previously shown that letrozole, an inhibitor of estrogen synthesis, increases Sertoli cell number and testicular size at sexual maturity in boars. These studies were conducted to determine whether letrozole affects the first or second proliferative wave. Boars were treated with letrozole during the first wave (treatment at 1, 3, and 5 weeks), less frequently (1 week of age only, or 1 and 5 weeks), on postnatal day 1, or during the second wave (weeks 11–16). Sertoli cells were enumerated in testes and estrogen concentrations were evaluated in serum and testes. Compared with vehicle controls, letrozole reduced estrogen in boars treated at weeks 1 and 5 or 1, 3, and 5, on postnatal day 1, or prepubertally. However, Sertoli cell numbers were increased only in boars treated at 1, 3, and 5 weeks of age. Neither perinatal (1 day old) nor prepubertal letrozole treatment affected Sertoli cell numbers. Hence, Sertoli cell proliferation was sensitive to letrozole only if letrozole was administered throughout the first wave, even though estrogen synthesis was effectively inhibited at all ages. These data indicate that the neonatal but not the prepubertal window of Sertoli cell proliferation is sensitive to an inhibitor of estrogen synthesis; this suggests that these two waves are differently regulated.

scholarly journals Stereological evaluation of Sertoli cell ontogeny during fetal and neonatal life in two diverse breeds of swine

2003 ◽  
Vol 178 (3) ◽  
pp. 395-403 ◽  
Author(s):  
SA McCoard ◽  
TH Wise ◽  
DD Lunstra ◽  
JJ Ford
Keyword(s):  
Sertoli Cell ◽  
Sertoli Cells ◽  
Prenatal Development ◽  
Late Gestation ◽  
Postnatal Life ◽  
Interstitial Tissue ◽  
Cell Numbers ◽  
Barrier Formation ◽  
Cell Population Growth ◽  
Testicular Size

Chinese Meishan (MS) boars have smaller testes due to fewer Sertoli cells compared with White Composite (WC) boars. The objective was to describe Sertoli cell development relative to circulating FSH concentrations in fetal and neonatal MS and WC boars. Testes and blood samples were collected on days 60, 75, 90 and 105 postcoitum (dpc) and 1, 7, 14 and 25 postpartum (dpp). One testis was immunostained for GATA4 or Ki67 antigen to evaluate total and proliferating Sertoli cell numbers respectively. Testicular size was greater (P<0.01) in WC than MS boars at all ages, associated with a greater mass of interstitial tIssue. Tubular mass (P<0.01) was greater in prenatal WC boars, but postnatally increased more rapidly (P<0.001) in MS boars, exceeding WC boars by 25 dpp. Sertoli cell numbers increased with age, was greater (P<0.001) in WC than MS boars during prenatal development but increased rapidly (P<0.01) by 1 dpp in MS and thereafter was similar in both breeds. The proportion of Ki67-positive Sertoli cells was maximal at 90 dpc, declining thereafter, did not differ between breeds through 7 dpp, but was greater (P<0.05) in WC than MS boars at 14 and 25 dpp. Plasma FSH concentrations were greater (P<0.05) in WC than MS boars at 75 dpc. FSH concentrations were elevated at 105 dpc (MS) and 1 dpp (WC) but declined thereafter with advancing postnatal age in both breeds. This study illustrates that late gestation represents the period of maximal Sertoli cell proliferation. Despite asynchronous Sertoli cell population growth between breeds during early postnatal life, differential mature Sertoli cell numbers and testicular size are probably due to differences in duration of the proliferative period after 25 dpp, potentially regulated by Sertoli cell maturation and blood-testis barrier formation. These events were not associated with fetal or early postnatal changes in FSH secretion.

Nutritional management during fetal and postnatal life, and the influence on testicular stereology and Sertoli cell numbers in Corriedale ram lambs

2001 ◽  
Vol 40 (1) ◽  
pp. 63-71 ◽  
Author(s):  
Alejandro Bielli ◽  
Helena Katz ◽  
Graciela Pedrana ◽  
Marı́a Teresa Gastel ◽  
Antonio Moraña ◽  
...  
Keyword(s):  
Sertoli Cell ◽  
Postnatal Life ◽  
Nutritional Management ◽  
Cell Numbers ◽  
Ram Lambs

Nutrition affects Sertoli cell function but not Sertoli cell numbers in sexually mature male sheep

2016 ◽  
Vol 28 (8) ◽  
pp. 1152 ◽  
Author(s):  
Yongjuan Guan ◽  
Guanxiang Liang ◽  
Penny A. R. Hawken ◽  
Sarah J. Meachem ◽  
Irek A. Malecki ◽  
...  
Keyword(s):  
Sertoli Cell ◽  
Sertoli Cells ◽  
Cell Function ◽  
Dietary Treatment ◽  
Cell Numbers ◽  
Under Nutrition ◽  
End Of Treatment ◽  
Effect Of Treatment ◽  
Sexually Mature ◽  
Male Sheep

We tested whether the reversible effects of nutrition on spermatogenesis in sexually mature sheep were mediated by Sertoli cells. Rams were fed with diets designed to achieve a 10% increase (High), no change (Maintenance) or a 10% decrease (Low) in body mass after 65 days. At the end of treatment, testes were lighter in the Low than the High group (P < 0.01). The Maintenance group had intermediate values that were not significantly different from those of the other two groups. Spermatogenesis (Johnsen score) was impaired in the Low group, but normal in both other groups. There was no effect of treatment on Sertoli cell numbers, although 1% of Sertoli cells appeared to retain their ability to proliferate. By contrast, Sertoli cell function was affected by dietary treatment, as evidenced by differences between the High and Low groups (P < 0.05) in the expression of seven Sertoli cell-specific genes. Under-nutrition appeared to reverse cellular differentiation leading to disruption of tight-junction morphology. In conclusion, in sexually mature sheep, reversible reductions in testis mass and spermatogenesis caused by under-nutrition were associated with impairment of basic aspects of Sertoli cell function but not with changes in the number of Sertoli cells.

Testicular toxicity and reduced sertoli cell numbers in neonatal rats by di(2-ethylhexyl) phthalate and the recovery of fertility as adults

1988 ◽  
Vol 95 (1) ◽  
pp. 104-121 ◽  
Author(s):  
Lori A. Dostal ◽  
Robert E. Chapin ◽  
Steven A. Stefanski ◽  
Martha W. Harris ◽  
Bernard A. Schwetz
Keyword(s):  
Sertoli Cell ◽  
Testicular Toxicity ◽  
Neonatal Rats ◽  
Cell Numbers ◽  
Ethylhexyl Phthalate
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