Differential role of TGF[beta]2 during follicle activation and preantral follicle growth

2014 ◽  
Author(s):  
Elizabeth Oliver ◽  
Mhairi Laird ◽  
Stephen Franks ◽  
Kate Hardy
Keyword(s):  
Tgf Beta ◽  
Preantral Follicle ◽  
Follicle Growth ◽  
Beta 2 ◽  
Follicle Activation

Mesoderm induction in amphibians: the role of TGF-beta 2-like factors

Science ◽  
1988 ◽  
Vol 239 (4841) ◽  
pp. 783-785 ◽  
Author(s):  
F Rosa ◽  
A. Roberts ◽  
D Danielpour ◽  
L. Dart ◽  
M. Sporn ◽  
...  
Keyword(s):  
Mesoderm Induction ◽  
Tgf Beta ◽  
Beta 2

Role of TGF beta s and BMPs as signals controlling the position of the digits and the areas of interdigital cell death in the developing chick limb autopod

Development ◽  
1996 ◽  
Vol 122 (8) ◽  
pp. 2349-2357 ◽  
Author(s):  
Y. Ganan ◽  
D. Macias ◽  
M. Duterque-Coquillaud ◽  
M.A. Ros ◽  
J.M. Hurle
Keyword(s):  
Gene Expression ◽  
Cell Death ◽  
Ectopic Expression ◽  
Local Administration ◽  
Tgf Beta ◽  
Signal Characteristic ◽  
Beta 2 ◽  
Interdigital Cell Death ◽  
Ectopic Area

The establishment of the digital rays and the interdigital spaces in the developing limb autopod is accompanied by the occurrence of corresponding domains of expression of TGF beta s and BMPs. This study analyzes whether these coincident events are functionally correlated. The experiments consisted of local administration of TGF beta-1, TGF beta-2 or BMP-4 by means of heparin or Affi-gel blue beads to the chick limb autopod in the stages preceding the onset of interdigital cell death. When beads bearing either TGF beta-1 or −2 were implanted in the interdigits, the mesodermal cells were diverted from the death program forming ectopic cartilages or extra digits in a dose- and stage-dependent fashion. This change in the interdigital phenotype was preceded by a precocious ectopic expression of ck-erg gene around the bead accompanied by down-regulation of bmp-4, msx-1 and msx-2 gene expression. When BMP-beads were implanted in the interdigital spaces, programmed cell death and the freeing of the digits were both accelerated. Implantation of beads bearing BMP-4 at the tip of the growing digits was followed by digit bifurcation, accompanied by the formation of an ectopic area of cell death resembling an extra interdigit, both morphologically and molecularly. The death-inducing effect of the BMP beads and the chondrogenic-inducing effect of the TGF beta beads were antagonized by the implantation of an additional bead preabsorbed with FGF-2, which constitutes a signal characteristic of the progress zone. It is concluded that the spatial distribution of digital rays and interdigital spaces might be controlled by a patterned distribution of TGF beta s and BMPs in the mesoderm subjacent to the progress zone.

scholarly journals Growth Differentiation Factor 9 Promotes Rat Preantral Follicle Growth by Up-Regulating Follicular Androgen Biosynthesis

Endocrinology ◽  
2009 ◽  
Vol 150 (6) ◽  
pp. 2740-2748 ◽  
Author(s):  
Makoto Orisaka ◽  
Jin-Yi Jiang ◽  
Sanae Orisaka ◽  
Fumikazu Kotsuji ◽  
Benjamin K. Tsang
Keyword(s):  
Cell Communication ◽  
Follicular Development ◽  
Preantral Follicle ◽  
Follicle Growth ◽  
Androgen Receptor Antagonist ◽  
Differentiation Factor ◽  
Growth Differentiation Factor 9 ◽  
Antisense Oligos

The transition from preantral to early antral stage is the penultimate stage of ovarian follicular development in terms of gonadotropin dependence and follicle destiny. Although oocyte-somatic cell communication is important in early follicular development, our knowledge of the precise role of the oocyte-derived growth differentiation factor (GDF)-9 during preantral follicle growth is incomplete. We examined whether and by what means oocyte-derived GDF-9 controls follicular development and steroidogenesis during the preantral to early antral transition, by a combination of in vitro gene manipulation (i.e. intraoocyte injection of GDF-9 antisense oligos) and preantral follicle culture. Intraoocyte injection of GDF-9 antisense suppressed rat preantral follicle growth in vitro, whereas GDF-9 enhanced follicular development. GDF-9 augmented testosterone production in preantral follicles. GDF-9 antisense suppressed androgen production and CYP17A1 mRNA expression in cultured follicles, a response attenuated by exogenous GDF-9. The nonaromatizable androgen 5α-dihydrotestosterone rescued the follicular growth arrest caused by GDF-9 down-regulation. The specific androgen receptor antagonist flutamide suppressed GDF-9-induced preantral follicle growth in vitro. The data suggest that GDF-9 plays an important role in promoting preantral follicle growth by up-regulating follicular androgen biosynthesis. GDF-9 is essential for CYP17A1 expression during follicular development from the preantral to the early antral stage.

scholarly journals C-type natriuretic peptide enhances mouse preantral follicle growth

Reproduction ◽  
2019 ◽  
Vol 157 (5) ◽  
pp. 445-455 ◽  
Author(s):  
Guangyin Xi ◽  
Wenjing Wang ◽  
Sarfaraz A Fazlani ◽  
Fusheng Yao ◽  
Mingyao Yang ◽  
...  
Keyword(s):  
Natriuretic Peptide ◽  
Mechanistic Study ◽  
Preantral Follicle ◽  
Follicle Growth ◽  
Independent Manner ◽  
Preantral Follicles ◽  
Estrogen Synthesis

Compared to ovarian antral follicle development, the mechanism underlying preantral follicle growth has not been well documented. Although C-type natriuretic peptide (CNP) involvement in preantral folliculogenesis has been explored, its detailed role has not been fully defined. Here, we used mouse preantral follicles and granulosa cells (GCs) as a model for investigating the dynamic expression of CNP and natriuretic peptide receptor 2 (NPR2) during preantral folliculogenesis, the regulatory role of oocyte-derived growth factors (ODGFs) in natriuretic peptide type C (Nppc) and Npr2 expression, and the effect of CNP on preantral GC viability. Both mRNA and protein levels of Nppc and Npr2 were gradually activated during preantral folliculogenesis. CNP supplementation in culture medium significantly promoted the growth of in vitro-cultured preantral follicles and enhanced the viability of cultured GCs in a follicle-stimulating hormone (FSH)-independent manner. Using adult and prepubertal mice as an in vivo model, CNP pre-treatment via intraperitoneal injection before conventional superovulation also had a beneficial effect on promoting the ovulation rate. Furthermore, ODGFs enhanced Nppc and Npr2 expression in the in vitro-cultured preantral follicles and GCs. Mechanistic study demonstrated that the regulation of WNT signaling and estrogen synthesis may be implicated in the promoting role of CNP in preantral folliculogenesis. This study not only proves that CNP is a critical regulator of preantral follicle growth, but also provides new insight in understanding the crosstalk between oocytes and somatic cells during early folliculogenesis.

Alterations in somite patterning of Myf-5-deficient mice: a possible role for FGF-4 and FGF-6

Development ◽  
1996 ◽  
Vol 122 (1) ◽  
pp. 141-150 ◽  
Author(s):  
S. Grass ◽  
H.H. Arnold ◽  
T. Braun
Keyword(s):  
Potential Role ◽  
Perinatal Death ◽  
Close Contact ◽  
Substantial Reduction ◽  
Tgf Beta ◽  
Targeted Mutation ◽  
Myogenic Factor ◽  
Beta 2 ◽  
Deficient Mice

Mice carrying a targeted mutation in the gene for the myogenic factor Myf-5 fail to form major parts of the ribs, which leads to an unstable thorax and perinatal death. Here, we report that somites of Myf-5-deficient mice lack the expression of FGF-4 and FGF-6 while TGF beta-2 is expressed normally. Early sclerotomal markers, such as Pax-1 reveal no substantial reduction of sclerotome size. At E11.5 the condensing mesenchyme of the rib anlagen is considerably reduced in size in Myf-5 mutant mice. This may be caused by the lack of Myf-5-positive, FGF-expressing cells which normally are in close contact with the lateral sclerotome generating the rib progenitors. The potential role of FGFs and TGF beta on sclerotome formation is demonstrated in micromass cultures of early somites. Combinations of FGF-4 or FGF-6 with TGF beta-2 potentiate chondrogenesis suggesting that these growth factors emanating from early myotomal and dermomyotomal cells may have instructive or permissive effects on differentiation or outgrowth of sclerotomal cells.

P11 The Role of Smooth Muscle Integrin Alpha V and TGF-Beta Pathways in Vascular Fibrosis

2020 ◽  
Vol 25 (Supplement 1) ◽  
pp. S54
Author(s):  
Alexandre Raoul ◽  
Ekaterina Belozertseva ◽  
Huguette Louis ◽  
Zhenlin Li ◽  
Veronique Regnault ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Tgf Beta
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