The development of a novel mouse embryonic ovary culture

2014 ◽  
Author(s):  
Agnes Stefansdottir ◽  
Ian Adams ◽  
Norah Spears
Keyword(s):  
Ovary Culture ◽  
Embryonic Ovary

Embryogenesis and Plant Regeneration from Unpollinated Ovary Culture of Lily

2015 ◽  
Vol 50 (3) ◽  
pp. 378
Author(s):  
Yuan Suxia ◽  
Li Jia ◽  
Ming Jun ◽  
Liu Chun ◽  
Xu Leifeng ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Ovary Culture

Seed and protocorm development by in vitro ovary culture at the fertilization phase in Cymbidium

2019 ◽  
pp. 141-148
Author(s):  
J. Kato ◽  
R. Hirai ◽  
K. Iguchi ◽  
A. Iwata ◽  
S. Ichihashi
Keyword(s):  
Ovary Culture

scholarly journals Shoots formation from gynosinesis Cucumis sativus l.

2020 ◽  
Vol 8 (1) ◽  
pp. 53-59
Author(s):  
Le Thi Kim Ngoc ◽  
Nguyen Tran Dong Phuong
Keyword(s):  
Cucumis Sativus ◽  
Callus Induction ◽  
Female Flower ◽  
Ovary Culture ◽  
Cold Pretreatment ◽  
Cucumis Sativus L ◽  
Leaves And Roots ◽  
Dark Conditions ◽  
Haploid Plants

Haploid plants achieve through androgenesis or gynogenesis. In gynogenesis method, the ovary or ovule are used as explants induct haploid plants. Female flower one day before flowering of Cucumis sativus L. are collected. Cold pretreatment of ovaries at 4°C up to 24 hours and culture under dark conditions. Significantly enhanced callus induction response is compared with cultures under 4-week cultured on CBM medium supplemented with various concentration of TDZ 0.01-0.04 mg/L. After 4 weeks, ovaries are transferred to medium with kinetin 0.05 – 0.20 mg/L. Then, ovaries were transferred to medium supplemented with BA: IAA 3:1. Finally, green ovaries were transferred to BA 1.5 mg/L and GA3 1.5 mg/L. The results showed that ovary induction has best affected on CBM with TDZ 0.03 mg/L with 11 callus/sample. Ovaries developed on kinetin 0.1 mg/L with 7.4 callus/sample. Ovaries become green and had leaves and roots formation on BA: IAA (3 mg/L: 1 mg/L). 11 plantlets were harvested from ovary culture after 12-week culture on CBM supplemented with BA 1.5 mg/L and GA3 1.5 mg/L.

Production of interspecific hybrids between Allium fistulosum L. and A. macrostemon Bunge through ovary culture

2006 ◽  
Vol 87 (3) ◽  
pp. 297-304 ◽  
Author(s):  
Mikihisa Umehara ◽  
Takayuki Sueyoshi ◽  
Katsumi Shimomura ◽  
Takao Nakahara
Keyword(s):  
Interspecific Hybrids ◽  
Allium Fistulosum ◽  
Ovary Culture
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