Inhibin [alpha] induced porcine granulosa cells apoptosis through mitochondrial apoptotic pathway

2014 ◽  
Author(s):  
Wan-Hong Li ◽  
Shu-Xiong Chen ◽  
Lu Chen ◽  
Chunjin Li ◽  
Xu Zhou
Keyword(s):  
Granulosa Cells ◽  
Apoptotic Pathway ◽  
Mitochondrial Apoptotic Pathway ◽  
Porcine Granulosa Cells

scholarly journals The Scutellarin Protects Mouse Ovarian Granulosa Cells from ZEA-Induced Injury

2020 ◽  
Author(s):  
Yanyan Yi ◽  
Shuangxiu Wan ◽  
Shaoyu Wang ◽  
Ajab Khan ◽  
Jianhua Guo ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Granulosa Cells ◽  
Caspase 3 ◽  
Reproductive Toxicity ◽  
Cycle Phase ◽  
Therapeutic Drug ◽  
Apoptotic Pathway ◽  
Mitochondrial Apoptotic Pathway ◽  
Ovarian Granulosa Cells ◽  
Induced Apoptosis

Abstract BackgroundThe zearalenone (ZEA) contained in the animal grain feeds is produced by Fusarium fungi and this toxin targets ovarian granulosa cells (GCs) to cause reproductive disorders in female animals. Current research on drugs that can rescue ZEA-induced GCs damage is limited. The purpose of this study was to explore the effect of scutellarin (Scu) on ZEA-induced apoptosis of mouse ovarian GCs and its mechanism.ResultsIn one set of experiments, the primary cultured mouse ovarian GCs were co-treated with ZEA and scutellarin for 24 h. The results showed that Scu significantly alleviated ZEA-induced cell damage, restored cell cycle arrest, and inhibited apoptosis by reducing the ratio of cleaved-caspase-3, cleaved-PARP, and Bax/Bcl-2. In other set of experiments, six weeks old mice were intragastrical administered with 40 mg/kg ZEA for 2 h, followed by 100 mg/kg Scu for 3 d. It was shown that Scu inhibited ZEA-induced apoptosis and positive signal expression of cleared-caspase-3 in the ovarian granulosa layer, with the involvement of mitochondrial apoptotic pathway. ConclusionScu attenuated ZEA-induced reproductive toxicity by targeting mouse ovarian GCs, mainly affecting cell cycle phase distribution and apoptosis via mitochondrial apoptotic pathway in vitro and in vivo. These data provide strong evidence that Scu can be further developed as potential new therapeutic drug for preventing or treating reproductive toxicity caused by animal exposure to ZEA found in the grains of animal feeds.

scholarly journals Over-expression of pemt2 into rat hepatoma cells contributes to the mitochondrial apoptotic pathway

IUBMB Life ◽  
2009 ◽  
Vol 61 (8) ◽  
pp. 846-852 ◽  
Author(s):  
Yali Li ◽  
Wei Zou ◽  
Qiu Yan ◽  
Yuefei Xu ◽  
Quan Xia ◽  
...  
Keyword(s):  
Hepatoma Cells ◽  
Apoptotic Pathway ◽  
Mitochondrial Apoptotic Pathway ◽  
Over Expression ◽  
Rat Hepatoma ◽  
Rat Hepatoma Cells

RGD-mediated adhesion of porcine granulosa cells modulates their differentiation response to FSH in vitro

1995 ◽  
Vol 83 (2-3) ◽  
pp. 169-177 ◽  
Author(s):  
Béatrice Goxe ◽  
Jacques E. Flechon ◽  
Solange Delasalle ◽  
Roland Salesse
Keyword(s):  
Granulosa Cells ◽  
Porcine Granulosa Cells

COMPARISON OF THE STIMULATORY EFFECTS OF OVINE, PORCINE AND HUMAN FOLLICLE-STIMULATING HORMONE AND OF OVINE AND HUMAN LUTEINIZING HORMONE ON THE ACCUMULATION OF CYCLIC AMP BY PORCINE GRANULOSA CELLS

1979 ◽  
Vol 80 (1) ◽  
pp. 9-20 ◽  
Author(s):  
ADA M. LINDSEY ◽  
CORNELIA P. CHANNING
Keyword(s):  
Cyclic Amp ◽  
Granulosa Cells ◽  
Incubation Medium ◽  
Follicle Stimulating Hormone ◽  
Similar Response ◽  
Intracellular Accumulation ◽  
Porcine Granulosa Cells ◽  
Tenfold Increase ◽  
Incubation Periods

The effects of ovine, porcine and human FSH, and ovine and human LH on the accumulation of cyclic AMP by porcine granulosa cells obtained from follicles at various stages of maturation were investigated. During incubation periods of 15 min, 10 μg ovine FSH pretreated with antiserum to LH or 10 μg human FSH resulted in an 11- to 18-fold, five-to ninefold, and less than a twofold increase in intracellular accumulation of cyclic AMP by granulosa cells from small (1–2 mm), medium (3–5 mm) and large (6–12 mm) follicles respectively. Similar patterns of response occurred with addition of porcine FSH. After incubation for 30 and 60 min with ovine, porcine or human FSH, significant accumulation of cyclic AMP in the incubation medium occurred with cells obtained from small and medium-sized follicles. After 60 min of incubation with FSH the accumulation of cyclic AMP in the incubation medium exceeded the intracellular cyclic AMP levels in granulosa cells from small and medium-sized follicles. During incubation periods of 15 min, 1·0 μg ovine LH resulted in less than a twofold, a fourfold and greater than a tenfold increase in intracellular accumulation of cyclic AMP by granulosa cells from small, medium and large follicles respectively. Addition of human LH brought about a similar response. Incubation periods of 30 and 60 min with 1·0 μg ovine or human LH resulted in significant accumulation of cyclic AMP in the incubation medium by granulosa cells from large follicles; cyclic AMP content in the incubation medium was greater after 60 min compared with 30 min of incubation. It was concluded that ovine FSH pretreated with an antiserum to LH had similar effects on cyclic AMP levels as did purified human and porcine FSH, and that the stimulatory effects of the less pure ovine FSH were probably not due to an impurity in the FSH preparation. Porcine granulosa cells obtained from small follicles should be suitable as an in-vitro FSH bioassay while granulosa cells obtained from large follicles should be suitable as an in-vitro LH bioassay.

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