The effect of cRNA concentration of artificial nuclease microinjected cytoplasmically to pronuclear porcine embryos on survival and development in vitro

2014 ◽  
Author(s):  
Maki Kamoshita ◽  
Tsubasa Kato ◽  
Eri Sagara ◽  
S Hisamatsu ◽  
M Sakaue ◽  
...  
Keyword(s):  
Survival And Development ◽  
Artificial Nuclease ◽  
Development In Vitro

scholarly journals STUDIES ON THE EXTRACELLULAR CULTIVATION OF AN INTRACELLULAR PARASITE (AVIAN MALARIA)

1952 ◽  
Vol 96 (5) ◽  
pp. 465-476 ◽  
Author(s):  
William Trager
Keyword(s):  
Malic Acid ◽  
Avian Malaria ◽  
Culture Medium ◽  
Coenzyme A ◽  
Intracellular Parasite ◽  
Sodium Pyruvate ◽  
Plasmodium Lophurae ◽  
Survival And Development ◽  
Development In Vitro

The extracellular survival and development in vitro of the erythrocytic stages of Plasmodium lophurae were favored by the addition to the culture medium of l-malic acid and concentrates rich in coenzyme A. In a concentrated extract of duck erythrocytes supplemented with these two substances in addition to adenosinetriphosphate, sodium pyruvate, and certain other materials of like nature, only 5 to 10 per cent of the extracellular parasites had become abnormal after 3 days of cultivation.

scholarly journals Progesterone and Follicle Stimulating Hormone interact and promote goat preantral follicles survival and development in vitro

2012 ◽  
Vol 32 (4) ◽  
pp. 361-367 ◽  
Author(s):  
Isabel B. Lima-Verde ◽  
Maria H.T. Matos ◽  
Juliana J.H. Celestino ◽  
Rafael Rossetto ◽  
Khesller P.O. Name ◽  
...  
Keyword(s):  
Ovarian Tissue ◽  
Follicle Stimulating Hormone ◽  
Primordial Follicles ◽  
Preantral Follicles ◽  
Ultrastructural Studies ◽  
Survival And Development ◽  
Survival And Growth ◽  
Development In Vitro ◽  
Stimulating Hormone

We investigated the effects of progesterone and follicle stimulating hormone (FSH) on survival and growth of caprine preantral follicles. Pieces of ovarian tissue were cultured for 1 or 7 days in minimum essential medium (MEM) alone or containing progesterone (1, 2.5, 5, 10 or 20ng/mL), FSH (50ng/mL) or the interaction between progesterone and FSH. Fresh (non-cultured control) and cultured ovarian tissues were processed for histological and ultrastructural studies. After 7 days the addition of FSH to all progesterone concentrations maintained the percentage of normal follicles similar to fresh control. At day 7 of culture, a higher percentage of developing follicles was observed only in 2.5ng/ml of progesterone associated with FSH or 10ng/ml of progesterone alone when compared with control. From day 1 to day 7 of culture, a significant increase in the percentage of developing follicles was observed in MEM and 2.5ng/ml of progesterone + FSH. In addition, after 7 days, in all treatments, there was a significant increase in follicular diameter when compared with control, except for MEM alone and in 5ng/ml of progesterone + FSH or 10ng/ml of progesterone alone. Ultrastructural studies confirmed follicular integrity after 7 days of culture in 2.5ng/ml of progesterone with FSH. In conclusion, this study demonstrated that the interaction between progesterone and FSH maintains ultrastructural integrity, stimulates primordial follicles activation and further growth of cultured caprine preantral follicles.

scholarly journals FURTHER STUDIES ON THE SURVIVAL AND DEVELOPMENT IN VITRO OF A MALARIAL PARASITE

1943 ◽  
Vol 77 (5) ◽  
pp. 411-420 ◽  
Author(s):  
William Trager
Keyword(s):  
Salt Solution ◽  
Cell Extract ◽  
Malarial Parasite ◽  
Red Cell ◽  
Calcium Pantothenate ◽  
Plasmodium Lophurae ◽  
Survival And Development ◽  
Gentle Agitation ◽  
Development In Vitro

The survival of Plasmodium lophurae in vitro is favored by the presence of calcium pantothenate (0.02 mg. per ml). Survival of about 2 weeks in vitro at 40–41°C. has been obtained under the following conditions: a medium consisting of duck red cell extract in balanced salt solution with glutathione and glucose or glycogen, serum, embryo extract, and calcium pantothenate; daily replacement of about half of the medium with fresh medium; addition of fresh uninfected erythrocytes every 2nd day; gentle agitation of the preparation on a rocking machine. In some of these preparations significant increases in male gametocytes and, more rarely, in total numbers of parasites occurred during the first few days.

The effect of development in vitro

2014 ◽  
Author(s):  
Hitomi Obata ◽  
Maki Kamoshita ◽  
Tsubasa Kato ◽  
Junya Ito ◽  
Naomi Kashiwazaki
Keyword(s):  
Development In Vitro

scholarly journals Nobiletin enhances the development and quality of bovine embryos in vitro during two key periods of embryonic genome activation

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Karina Cañón-Beltrán ◽  
Yulia N. Cajas ◽  
Serafín Peréz-Cerezales ◽  
Claudia L. V. Leal ◽  
Ekaitz Agirregoitia ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Signaling Pathway ◽  
Akt Signaling ◽  
Mitochondrial Activity ◽  
Bovine Embryos ◽  
Akt Signaling Pathway ◽  
Cell Stage ◽  
Development In Vitro

AbstractIn vitro culture can alter the development and quality of bovine embryos. Therefore, we aimed to evaluate whether nobiletin supplementation during EGA improves embryonic development and blastocyst quality and if it affects PI3K/AKT signaling pathway. In vitro zygotes were cultured in SOF + 5% FCS (Control) or supplemented with 5, 10 or 25 µM nobiletin (Nob5, Nob10, Nob25) or with 0.03% dimethyl-sulfoxide (CDMSO) during minor (2 to 8-cell stage; MNEGA) or major (8 to 16-cell stage; MJEGA) EGA phase. Blastocyst yield on Day 8 was higher in Nob5 (42.7 ± 1.0%) and Nob10 (44.4 ± 1.3%) for MNEGA phase and in Nob10 (61.0 ± 0.8%) for MJEGA phase compared to other groups. Mitochondrial activity was higher and lipid content was reduced in blastocysts produced with nobiletin, irrespective of EGA phase. The mRNA abundance of CDK2, H3-3B, H3-3A, GPX1, NFE2L2 and PPARα transcripts was increased in 8-cells, 16-cells and blastocysts from nobiletin groups. Immunofluorescence analysis revealed immunoreactive proteins for p-AKT forms (Thr308 and Ser473) in bovine blastocysts produced with nobiletin. In conclusion, nobiletin supplementation during EGA has a positive effect on preimplantation bovine embryonic development in vitro and corroborates on the quality improvement of the produced blastocysts which could be modulated by the activation of AKT signaling pathway.

scholarly journals Analysis of Morphokinetic Parameters of Feline Embryos Using a Time-Lapse System

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 748
Author(s):  
Joanna Kochan ◽  
Agnieszka Nowak ◽  
Barbara Kij ◽  
Sylwia Prochowska ◽  
Wojciech Niżański
Keyword(s):  
Embryo Development ◽  
Embryo Quality ◽  
Time Lapse ◽  
Blastocyst Stage ◽  
Cavity Formation ◽  
Non Invasive ◽  
Morphokinetic Parameters ◽  
Morphological Defects ◽  
Development In Vitro

The aim of this study was to analyze the morphokinetic parameters of feline embryos using a time lapse system. Oocytes matured in vitro were fertilized (IVF) and in vitro cultured in a time lapse-system (Primo Vision®, Gothenburg, Sweden). The first cell division of embryos occurred between 17 h post insemination (hpi) and 38 hpi, with the highest proportion of embryos (46%) cleaving between 21 and 24 hpi. The timing of the first cleavage significantly affected further embryo development, with the highest development occurring in embryos that cleaved at 21–22 hpi. Embryos that cleaved very early (17–18 hpi) developed poorly to the blastocyst stage (2%) and none of the embryos that cleaved later than 27 hpi were able to reach the blastocyst stage. Morphological defects were observed in 48% of the embryos. There were no statistically significant differences between the timing intervals of the first cleavage division and the frequency of morphological defects in embryos. Multiple (MUL) morphological defects were detected in more than half (56%) of the abnormal embryos. The most frequent single morphological defects were cytoplasmic fragmentation (FR) (8%) and blastomere asymmetry (AS) (6%). Direct cleavage (DC) from 1–3 or 3–5 blastomeres, reverse cleavage (RC) and vacuoles were rarely observed (2–3%). The timing of blastocyst cavity formation is a very good indicator of embryo quality. In our study, blastocyst cavity formation occurred between 127–167 hpi, with the highest frequency of hatching observed in blastocysts that cavitated between 142–150 hpi. Blastocysts in which cavitation began after 161 h did not hatch. In conclusion, the timing of the first and second cleavage divisions, the timing of blastocyst cavity formation and morphological anomalies can all be used as early and non-invasive indicators of cat embryo development in vitro.

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