scholarly journals Luteinization of porcine preovulatory follicles leads to systematic changes in follicular gene expression

Reproduction ◽  
2006 ◽  
Vol 132 (1) ◽  
pp. 133-145 ◽  
Author(s):  
Cansu Agca ◽  
James E Ries ◽  
Sarah J Kolath ◽  
Jae-Hwan Kim ◽  
Lawrence J Forrester ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Structural Changes ◽  
Ovarian Follicles ◽  
Cdna Libraries ◽  
Corpora Lutea ◽  
Examine Gene Expression ◽  
Preovulatory Follicles ◽  
Migratory Cells

The LH surge initiates the luteinization of preovulatory follicles and causes hormonal and structural changes that ultimately lead to ovulation and the formation of corpora lutea. The objective of the study was to examine gene expression in ovarian follicles (n= 11) collected from pigs (Sus scrofa domestica) approaching estrus (estrogenic preovulatory follicle;n= 6 follicles from two sows) and in ovarian follicles collected from pigs on the second day of estrus (preovulatory follicles that were luteinized but had not ovulated;n= 5 follicles from two sows). The follicular status within each follicle was confirmed by follicular fluid analyses of estradiol and progesterone ratios. Microarrays were made from expressed sequence tags that were isolated from cDNA libraries of porcine ovary. Gene expression was measured by hybridization of fluorescently labeled cDNA (preovulatory estrogenic or -luteinized) to the microarray. Microarray analyses detected 107 and 43 genes whose expression was decreased or increased (respectively) during the transition from preovulatory estrogenic to -luteinized (P<0.01). Cells within preovulatory estrogenic follicles had a gene-expression profile of proliferative and metabolically active cells that were responding to oxidative stress. Cells within preovulatory luteinized follicles had a gene-expression profile of nonproliferative and migratory cells with angiogenic properties. Approximately, 40% of the discovered genes had unknown function.

scholarly journals Distinct profiles of expressed sequence tags during intestinal regeneration in the sea cucumberHolothuria glaberrima

2007 ◽  
Vol 31 (2) ◽  
pp. 203-215 ◽  
Author(s):  
Carmencita Rojas-Cartagena ◽  
Pablo Ortíz-Pineda ◽  
Francisco Ramírez-Gómez ◽  
Edna C. Suárez-Castillo ◽  
Vanessa Matos-Cruz ◽  
...  
Keyword(s):  
Gene Expression ◽  
Differential Expression ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Molecular Mechanisms ◽  
Cdna Libraries ◽  
Rt Pcr ◽  
Protein Database ◽  
Intestinal Regeneration ◽  
Expressed Sequence

Repair and regeneration are key processes for tissue maintenance, and their disruption may lead to disease states. Little is known about the molecular mechanisms that underline the repair and regeneration of the digestive tract. The sea cucumber Holothuria glaberrima represents an excellent model to dissect and characterize the molecular events during intestinal regeneration. To study the gene expression profile, cDNA libraries were constructed from normal, 3-day, and 7-day regenerating intestines of H. glaberrima. Clones were randomly sequenced and queried against the nonredundant protein database at the National Center for Biotechnology Information. RT-PCR analyses were made of several genes to determine their expression profile during intestinal regeneration. A total of 5,173 sequences from three cDNA libraries were obtained. About 46.2, 35.6, and 26.2% of the sequences for the normal, 3-days, and 7-days cDNA libraries, respectively, shared significant similarity with known sequences in the protein database of GenBank but only present 10% of similarity among them. Analysis of the libraries in terms of functional processes, protein domains, and most common sequences suggests that a differential expression profile is taking place during the regeneration process. Further examination of the expressed sequence tag dataset revealed that 12 putative genes are differentially expressed at significant level ( R > 6). Experimental validation by RT-PCR analysis reveals that at least three genes (unknown C-4677-1, melanotransferrin, and centaurin) present a differential expression during regeneration. These findings strongly suggest that the gene expression profile varies among regeneration stages and provide evidence for the existence of differential gene expression.

Gene Expression Profile in Persons Exposed to the WTC With and Without PTSD

2009 ◽  
Author(s):  
Rachel Yehuda ◽  
Julia Golier ◽  
Sandro Galea ◽  
Marcus Ising ◽  
Florian Holsborer ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profile ◽  
Expression Profile
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