scholarly journals Imprinted gene expression in the rat embryo–fetal axis is altered in response to periconceptional maternal low protein diet

Reproduction ◽  
2006 ◽  
Vol 132 (2) ◽  
pp. 265-277 ◽  
Author(s):  
Wing Yee Kwong ◽  
Daniel J Miller ◽  
Elizabeth Ursell ◽  
Arthur E Wild ◽  
Adrian P Wilkins ◽  
...  
Keyword(s):  
Gene Expression ◽  
Fetal Liver ◽  
Mrna Level ◽  
Postnatal Growth ◽  
Protein Diet ◽  
Low Protein Diet ◽  
Differentially Methylated Region ◽  
Imprinted Gene ◽  
Low Protein ◽  
Gender Specific

In our previous study, we have shown that maternal low protein diet (LPD, 9% casein vs 18% casein control) fed exclusively during the rat preimplantation period (0–4.25 day postcoitum) induced low birth weight, altered postnatal growth and hypertension in a gender-specific manner. In this study, we investigated the effect of maternal LPD restricted only to the preimplantation period (switched diet) or provided throughout gestation on fetal growth and imprinted gene expression in blastocyst and fetal stages of development. Male, but not female, blastocysts collected from LPD dams displayed a significant reduction (30%) inH19mRNA level. A significant reduction inH19(9.4%) andIgf2(10.9%) mRNA was also observed in male, but not in female, fetal liver at day 20 postcoitum in response to maternal LPD restricted to the preimplantation period. No effect on the blastocyst expression ofIgf2Rwas observed in relation to maternal diet. The reduction inH19mRNA expression did not correlate with an observed alteration in DNA methylation at theH19differentially methylated region in fetal liver. In contrast, maternal LPD throughout 20 days of gestation did not affect male or femaleH19andIgf2imprinted gene expression in fetal liver. Neither LPD nor switched diet treatments affectedH19andIgf2imprinted gene expression in day 20 placenta. Our findings demonstrate that one contributor to the alteration in postnatal growth induced by periconceptional maternal LPD may derive from a gender-specific programming of imprinted gene expression originating within the preimplantation embryo itself.

Maternal low protein diet restricted to the preimplantation period induces a gender-specific change on hepatic gene expression in rat fetuses

2006 ◽  
Vol 74 (1) ◽  
pp. 48-56 ◽  
Author(s):  
Wing Yee Kwong ◽  
Daniel J. Miller ◽  
Adrian P. Wilkins ◽  
Mark S. Dear ◽  
J. Neville Wright ◽  
...  
Keyword(s):  
Gene Expression ◽  
Protein Diet ◽  
Low Protein Diet ◽  
Hepatic Gene Expression ◽  
Specific Change ◽  
Rat Fetuses ◽  
Low Protein ◽  
Gender Specific

scholarly journals PSIV-6 Effect of a Phytogenic Water Additive on Growth Performance, Blood Metabolites and Gene Expression of Amino Acids Transporters in Nursery Pigs Fed with Low Protein Diets

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 281-282
Author(s):  
Cedrick N Shili ◽  
Mohammad Habibi ◽  
Julia Sutton ◽  
Jessie Barnes ◽  
Jacob Burchkonda ◽  
...  
Keyword(s):  
Gene Expression ◽  
Amino Acids ◽  
Growth Performance ◽  
Mrna Abundance ◽  
Protein Diet ◽  
Low Protein Diet ◽  
Blood Calcium ◽  
Low Protein ◽  
Protein Diets ◽  
Standard Protein

Abstract Moderately low protein (MLP) diets can help decrease nutrient excretion from the swine production. However, MLP diets negatively impact growth performance. We hypothesized that supplementing MLP diets with phytogenics may reduce the negative effects of these diets on growth. The objective of this study was to investigate the effect of a phytogenic water additive (PWA; Herbanimal®) on growth performance, blood metabolite and gene expression of amino acids transporters in pigs fed with MLP diets. Forty-eight weaned barrows were allotted to six dietary treatments (n = 8) for 4 weeks: >CON-NS: standard protein diet-no PWA; CON-LS: standard protein diet-low PWA dose (4 ml/L); CON-HS: standard protein diet-high PWA dose (8 ml/L); LP-NS: low protein diet-no PWA; LP-LS: low protein diet-low PWA dose (4 ml/L); LP-HS: low protein diet- high PWA dose (8 ml/L). Feed intake and body weight were recorded daily and weekly, respectively. At week 4, blood and tissue samples were collected and analyzed for metabolites using a chemistry analyzer and amino acid transporters using qPCR, respectively. The data were analyzed by univariate GLM (SPSS®) and the means were separated using paired Student’s t-test corrected by Benjamini-Hochberg. Pigs fed CON-HS improved the average daily gain and serum calcium and phosphorus concentrations compared to CON-NS. Pigs fed LP-LS had higher serum phosphorus and blood urea nitrogen compared to the pigs fed with LP-NS. The mRNA abundance of SLC7A11 in the jejunum was lower in CON-LS and CON-HS compared to CON-NS. Additionally, mRNA abundance of SLC6A19 in the jejunum of pigs fed with LP-LS was higher compared to LP-NS and lower in CON-HS relative to pigs fed with CON-LS. In conclusion, PWA improved the growth performance of pigs fed standard protein diets but not low protein diets. Further, the PWA improved the concentrations of blood calcium and phosphorous in pigs fed MLP diets. Funding: Agrivida and Animal Health and Production and Animal Products: Improved Nutritional Performance, Growth, and Lactation of Animals from the USDA-NIFA.

Effect of maternal low protein diet during pregnancy on the fetal liver of rats

2013 ◽  
Vol 195 (1) ◽  
pp. 68-76 ◽  
Author(s):  
Wafaa S. Ramadan ◽  
Ilham Alshiraihi ◽  
Saleh Al-karim
Keyword(s):  
Fetal Liver ◽  
Protein Diet ◽  
Low Protein Diet ◽  
Low Protein

scholarly journals Isocaloric maternal low-protein diet alters IGF-I, IGFBPs, and hepatocyte proliferation in the fetal rat

2003 ◽  
Vol 285 (5) ◽  
pp. E991-E1000 ◽  
Author(s):  
Ilham El Khattabi ◽  
Francine Grégoire ◽  
Claude Remacle ◽  
Brigitte Reusens
Keyword(s):  
Cell Proliferation ◽  
Fetal Liver ◽  
Protein Diet ◽  
Low Protein Diet ◽  
Hepatocyte Proliferation ◽  
Liver Growth ◽  
Fetal Plasma ◽  
Low Protein ◽  
Igf I

We investigated the effect of an isocaloric maternal low-protein diet during pregnancy in rats on the proliferative capacity of cultured fetal hepatocytes. The potential roles of these changes on the IGF-IGF-binding protein (IGFBP) axis, and the role of insulin and glucocorticoids in liver growth retardation, were also evaluated. Pregnant Wistar rats were fed a control (C) diet (20% protein) or a low-protein (LP) diet (8%) throughout gestation. In primary culture, the DNA synthesis of hepatocytes derived from LP fetuses was decreased by ∼30% compared with control hepatocytes ( P < 0.05). In parallel, in vivo moderate protein restriction in the dam reduced the fetal liver weight and IGF-I level in fetal plasma ( P < 0.01) and augmented the abundance of 29- to 32-kDa IGFBPs in fetal plasma ( P < 0.01) and fetal liver ( P < 0.01). By contrast, the abundance of IGF-II mRNA in liver of LP fetuses was unaffected by the LP diet. In vitro, the LP-derived hepatocytes produced less IGF-I ( P < 0.01) and more 29- to 32-kDa IGFBPs ( P < 0.01) than hepatocytes derived from control fetuses. These alterations still appeared after 3–4 days of culture, indicating some persistence in programming. Dexamethasone treatment of control-derived hepatocytes decreased cell proliferation (54 ± 2.3%, P < 0.01) and stimulated 29- to 32-kDa IGFBPs, whereas insulin promoted fetal hepatocyte growth (127 ± 5.5%, P < 0.01) and inhibited 29- to 32-kDa IGFBPs. These results show that liver growth and cell proliferation in association with IGF-I and IGFBP levels are affected in utero by fetal undernutrition. It also suggests that glucocorticoids and insulin may modulate these effects.

Effects of Low Protein Diet on Nuclear Factor Erythroid 2–Related Factor 2 Gene Expression in Nondialysis Chronic Kidney Disease Patients

2020 ◽  
Vol 30 (1) ◽  
pp. 46-52 ◽  
Author(s):  
Juliana Saraiva dos Anjos ◽  
Ludmila Ferreira Medeiros de França Cardozo ◽  
Ana Paula Black ◽  
Greicielle Santos da Silva ◽  
Drielly Cristhiny Mendes de Vargas Reis ◽  
...  
Keyword(s):  
Gene Expression ◽  
Chronic Kidney Disease ◽  
Kidney Disease ◽  
Protein Diet ◽  
Low Protein Diet ◽  
Related Factor ◽  
Nuclear Factor ◽  
Low Protein

A Maternal Low-protein Diet during Gestation Induces Hepatic Autophagy-related Gene Expression in a Sex-specific Manner in Sprague-Dawley Rats

2021 ◽  
pp. 1-29
Author(s):  
Mingzhu Cai ◽  
Jie Zhang ◽  
Hong Chen ◽  
Yuan-Xiang Pan
Keyword(s):  
Gene Expression ◽  
Transcription Factors ◽  
Control Diet ◽  
Protein Diet ◽  
Low Protein Diet ◽  
Related Gene ◽  
Sprague Dawley ◽  
Protein Levels ◽  
Low Protein ◽  
Sprague Dawley Rats

Abstract This study investigates the mechanism by which maternal protein restriction induces hepatic autophagy-related gene expression in the offspring of rats. Pregnant Sprague-Dawley rats were fed either a control diet (C, 18% energy from protein) or a low-protein diet (LP, 8.5% energy from protein) during gestation, followed by the control diet during lactation and post-weaning. Liver tissue was collected from the offspring at postnatal day 38 and divided into four groups according to sex and maternal diet (F-C, F-LP, M-C, and M-LP) for further analysis. Autophagy-related mRNA and protein levels were determined by real-time PCR and Western blotting, respectively. In addition, chromatin immunoprecipitation (ChIP) was performed to investigate the interactions between transcription factors and autophagy-related genes. Protein levels of p-eIF2a and ATF4 were increased only in the female offspring born to dams fed the LP diet. Correlatively, the mRNA expression of hepatic autophagy-related genes including Map1lc3b, P62/Sqstm1, Becn1, Atg3, Atg7, and Atg10 was significantly greater in the F-LP group than in the F-C group. Furthermore, ChIP results showed greater ATF4 and C/EBP homology protein (CHOP) binding at the regions of a set of autophagy-related genes in the F-LP group than in the F-C group. Our data demonstrated that a maternal LP diet transcriptionally programmed hepatic autophagy-related gene expression only in female rat offspring. This transcriptional program involved the activation of the eIF2α/ATF4 pathway and intricate regulation by transcription factors ATF4 and CHOP.

Sign in / Sign up

Export Citation Format

Share Document