scholarly journals Association between ovarian follicle development and pregnancy rates in dairy cows undergoing spontaneous oestrous cycles

Reproduction ◽  
2004 ◽  
Vol 127 (5) ◽  
pp. 621-629 ◽  
Author(s):  
Emma C L Bleach ◽  
Richard G Glencross ◽  
Philip G Knight
Keyword(s):  
Dairy Cows ◽  
Pregnancy Rate ◽  
Ovarian Follicle ◽  
Pregnancy Rates ◽  
Developmental Competence ◽  
Follicle Development ◽  
Ovarian Follicle Development ◽  
Ovulatory Follicle ◽  
Ovulatory Follicles ◽  
First Time

Ovarian follicle development continues in a wave-like manner during the bovine oestrous cycle giving rise to variation in the duration of ovulatory follicle development. The objectives of the present study were to determine whether a relationship exists between the duration of ovulatory follicle development and pregnancy rates following artificial insemination (AI) in dairy cows undergoing spontaneous oestrous cycles, and to identify factors influencing follicle turnover and pregnancy rate and the relationship between these two variables. Follicle development was monitored by daily transrectal ultrasonography from 10 days after oestrus until the subsequent oestrus in 158 lactating dairy cows. The cows were artificially inseminated following the second observed oestrus and pregnancy was diagnosed 35 days later. The predominant pattern of follicle development was two follicle waves (74.7%) with three follicle waves in 22.1% of oestrous cycles and four or more follicle waves in 3.2% of oestrous cycles. The interval from ovulatory follicle emergence to oestrus (EOI) was 3 days longer (P < 0.0001) in cows with two follicle waves than in those with three waves. Ovulatory follicles from two-wave oestrous cycles grew more slowly but were approximately 2 mm larger (P < 0.0001) on the day of oestrus. Twin ovulations were observed in 14.2% of oestrous cycles and occurred more frequently (P < 0.001) in three-wave oestrous cycles; consequently EOI was shorter in cows with twin ovulations. Overall, 57.0% of the cows were diagnosed pregnant 35 days after AI. Linear logistic regression analysis revealed an inverse relationship between EOI and the proportion of cows diagnosed pregnant, among all cows (n = 158; P < 0.01) and amongst those with single ovulations (n = 145; P < 0.05). Mean EOI was approximately 1 day shorter (P < 0.01) in cows that became pregnant than in non-pregnant cows; however, pregnancy rates did not differ significantly among cows with different patterns of follicle development. These findings confirm and extend previous observations in pharmacologically manipulated cattle and show, for the first time, that in dairy cows undergoing spontaneous oestrous cycles, natural variation in the duration of post-emergence ovulatory follicle development has a significant effect on pregnancy rate, presumably reflecting variation in oocyte developmental competence.

scholarly journals Effect of dietary-induced changes in plasma insulin concentrations during the early post partum period on pregnancy rate in dairy cows

Reproduction ◽  
2009 ◽  
Vol 137 (4) ◽  
pp. 759-768 ◽  
Author(s):  
P C Garnsworthy ◽  
A A Fouladi-Nashta ◽  
G E Mann ◽  
K D Sinclair ◽  
R Webb
Keyword(s):  
Dairy Cows ◽  
Pregnancy Rate ◽  
Plasma Insulin ◽  
Post Partum ◽  
Ovarian Follicle ◽  
Developmental Competence ◽  
Cyclic Activity ◽  
Plasma Insulin Concentration ◽  
Mating Period ◽  
Ovarian Follicle Development

Dietary stimulation of insulin in post partum dairy cows has been found to enhance ovarian follicle development but to impair oocyte developmental competence. The objective of this study was to test the hypothesis that pregnancy rate would be improved by feeding a diet to stimulate higher insulin (H) until cows resumed ovarian cyclic activity after parturition, and then feeding a diet to lower insulin (L) during the mating period. Each diet was fed to 30 post partum dairy cows until their first rise in milk progesterone, when 15 cows in each group were transferred to the other diet (treatments HL and LH) and 15 cows in each group remained on their original diet (treatments HH and LL) until 120 days post partum. Treatments did not affect dry matter intake, milk yield and metabolisable energy balance. Plasma insulin concentration was elevated in cows fed on H compared with cows fed on L. Treatment did not affect days to first progesterone rise, first oestrus or first insemination. At 120 days post partum, 27% of cows on each of treatments HH, LL and LH were pregnant, but 60% of cows on treatment HL were pregnant (P=0.021). These findings support the concept that physiological relationships between insulin and the reproductive system vary according to stage of the reproductive cycle, and suggest that pregnancy rate can be enhanced by a two-diet strategy tailored to optimise responses before and after the first post partum ovulation.

scholarly journals Molecular Characterization, Tissue Distribution, and Expression Profiling of the CTSD Gene during Goose Ovarian Follicle Development

2021 ◽  
Vol 69 (1) ◽  
pp. 39-48
Author(s):  
Jiaran Zhu ◽  
Shenqiang Hu ◽  
Yao Lu ◽  
Yujing Rong ◽  
Enhua Qing ◽  
...  
Keyword(s):  
Amino Acids ◽  
Expression Profiling ◽  
Cathepsin D ◽  
Expression Profiles ◽  
Ovarian Follicle ◽  
Reproductive Organs ◽  
Follicle Development ◽  
Ovarian Follicle Development ◽  
Ovarian Cells ◽  
First Time

Cathepsin D (CTSD) is known to be crucial for the degradation and utilization of yolk precursors in ovarian follicles. However, little is known about its expression profiles and physiological actions in avian ovarian cells. In this study, the intact coding sequence of the CTSD gene in geese was cloned for the first time, with a length of 1197 bp. It encoded a polypeptide of 398 amino acids (AA) consisting of a signal peptide and two conserved functional domains (i.e., A1_Propeptide and Cathepsin_D2). The AA sequence of goose CTSD had > 96% similarities with the homologs of turkeys, chickens, and ducks. Results from real-time PCR showed that goose CTSD mRNA was present in all tissues examined, with higher levels in the adrenal gland, liver, heart, and reproductive organs. Furthermore, levels of CTSD mRNA were much higher in goose granulosa layers than in the theca layers in any follicular category. Significantly, its expression remained almost unchanged in the theca layers throughout follicle development, while it increased gradually in the granulosa layers from 2-4 mm to F5 follicles but declined there after. These results suggested that CTSD may regulate goose ovarian follicle development through its actions on both the degradation and absorption of yolk precursors and granulosa cell apoptosis.

224 OVARIAN FOLLICLE DEVELOPMENT DURING SEASONAL TRANSITION IN WAPITI

2006 ◽  
Vol 18 (2) ◽  
pp. 220
Author(s):  
R. McCorkell ◽  
M. Woodbury ◽  
G. Adams
Keyword(s):  
Breeding Season ◽  
Ovarian Follicle ◽  
Follicular Development ◽  
Maximum Diameter ◽  
Follicle Development ◽  
Estrous Cycles ◽  
Ovarian Follicle Development ◽  
Array Transducer ◽  
October 17 ◽  
Ovulatory Follicles

Wapiti are seasonally polyestrous. The transition into and out of the breeding season is marked by resumption of ovulation in autumn and cessation of ovulation in winter. Onset of ovulatory cyclicity is distinct and associated with aggressive breeding behavior of stags in rut. Cessation of ovulation at the end of the breeding season is not distinguished by behavioral patterns. The objective of the present study was to characterize follicular and luteal dynamics in wapiti during the transitional periods into and out of the breeding seasons. Transition from anestrus to estrus was monitored in 15 hinds, aged 2 to 14 years, over two successive seasons (11 in year 1, with 5 hinds from year 1 used again in year 2 along with 4 new hinds; n = 20 observations). Transition from estrus to anestrus was monitored in 11 hinds over 1 season (n = 11 observations). Hinds were maintained on a farm near Saskatoon, Saskatchewan (52°07′N, 106°38′W). The ovaries were examined daily during September through October by transrectral ultrasonography using a B-mode ultrasound machine and a 7.5 MHz linear array transducer for transition to estrus, and December through April for transition to anestrus. The first ovulation was recorded on September 15 and all hinds had ovulated for the first time by October 7. In 17 of 20 observations, the duration of the first interovulatory interval (IOI) was 9.3 ± 0.4 days (mean ± SEM). With one exception, these IOIs were characterized by one wave of follicular development. The remaining three IOIs ranged from 16 to 23 days and consisted of two or three waves of follicle development. The second ovulation occurred by October 15 in hinds with a short IOI and by October 17 in all remaining hinds. The mean dates of first and last ovulation were September 25 and February 7, respectively, an interval of 135 days. The median date of the last ovulation was February 15 and the range was from December 3 to March 22. Duration of the last IOI of the season (21.2 ± 0.6 days) was similar to the notional 21-day cycle for wapiti, but longer (P < 0.05) than the duration of the first IOI (10.9 ± 1.0 days). Maximum diameters of the first 2 ovulatory follicles were similar (11.3 ± 0.4 vs. 11.3 ± 0.2 mm), but were larger (P < 0.05) than the last 2 ovulatory follicles of the breeding season (10.3 ± 0.3 vs. 10.1 ± 0.4 mm). Maximum diameter of the corpus luteum (CL) tended (P = 0.06) to be smaller for the short IOI than for longer IOI of the first and last cycles. Diameter of the last CL of the season was not different from that of the previous CL (12.8 ± 0.6 vs. 12.5 ± 0.6 mm); however, it was detected for a longer period (22.3 ± 1.2 vs. 19.3 ± 0.7 days; P < 0.05). Estrous cycles during transition into the breeding season have been described as being irregular and those out of the breeding season as increasingly long. In the present study, the transition periods were characterized by regular events. Transition to regular estrous cycles was preceded by one short (9 days) IOI. The last IOI of the breeding season was the same as that reported during the rut. Transition to anestrus occurred most commonly in February and was marked by a failure of the dominant follicle to ovulate after luteal regression.

Some effects of post-oestrous hormonal therapies on conception rates and resubmission rates in lactating dairy cows

2001 ◽  
Vol 26 (1) ◽  
pp. 195-208 ◽  
Author(s):  
K. L. Macmillan ◽  
V.K. Taufa ◽  
A.M. Day ◽  
V.M. Eagles
Keyword(s):  
Dairy Cows ◽  
Ovarian Follicle ◽  
Follicle Development ◽  
Early Lactation ◽  
Detection Rates ◽  
Ovarian Follicle Development ◽  
Lactating Dairy Cows ◽  
Conception Rates ◽  
Hormonal Therapies ◽  
The Impact

AbstractA range of hormonal therapies has been evaluated to potentially improve the reproductive performance of lactating dairy cows. Early lactation treatments with gonadotrophin releasing hormone (GnRH) or prostaglandin F2∝(PGF) may reduce the interval to first insemination or increase the conception rate to first insemination, but mainly in cows which have had a difficult pueperium or which are in herds with low conception rates. These two hormones, as well as progesterone and oestradiol benzoate (ODB) are commonly used either singly, or in combination (GnRH + PGF; progesterone + ODB + PGF) to synchronise the oestrus preceding first inseminations. None of these synchrony treatments is associated with increased conception rates. Extensive series of trials have been completed to identify post-oestrous or post-insemination hormonal therapies which could increase conception rates to the preceding insemination. The wide variation in results has precluded any being commonly regarded as sufficiently reliable for routine use. Nonetheless, meta-analyses have shown that GnRH treatment at insemination or in late dioestrus (11 to 13 day post-first insemination) can significantly increase “the risk of pregnancy”. Insemination treatments have been most effective with repeat breeders (+22.5%), whereas late dioestrous treatments (10%) may be dose and analogue specific (10 μg buserelin). Although metoestrous supplementation with progesterone can stimulate early embryonic development, the associated reduction in oestrous cycle length also reduces conception rates in heifers. Late dioestrous use of GnRH can prevent both of these negative effects. Early dioestrous supplementation with progesterone may enhance production of interferon tau, but this potentially beneficial effect has not been able to be reliably translated into increased conception rates. Many of these hormonal therapies are associated with altered patterns of ovarian follicle development which are similar to those in some synchrony treatments preceding first insemination. Recent studies have indicated that OBD and progesterone can be used to synchronise returns to service and increase the submission rate for second inseminations made about 3 weeks after first inseminations. This can make the non-return rate a more accurate measure of the response to a hormonal treatment and potentially overcome confusing impressions created when oestrous detection rates may be around 50%. Even if effective hormonal therapies are successfully developed, the results may be compromised by environmental factors such as heat stress, energy balance or energy partitioning for lactation. These factors may reduce oocyte quality, fertilization rates or normal uterine secretion patterns. Reduced conception rates associated with high daily milk yields in early lactation may not be able to be remedied simply with hormonal supplementation or by altering patterns of ovarian follicle development. Under these circumstances, controlling the inter-service interval could reduce the impact of the lowered conception rates.

Effects of active immunization against inhibin and androstenedione, separately or together, on hormone profiles, ovarian follicle populations and ovulation rate in heifers

1991 ◽  
Vol 1991 ◽  
pp. 17-17
Author(s):  
S.M. Rhind ◽  
S.R. Schemm ◽  
B.D. Schanbacher
Keyword(s):  
Ovarian Follicle ◽  
Pulse Frequency ◽  
Active Immunization ◽  
Follicular Phase ◽  
Ovulation Rate ◽  
Follicle Development ◽  
Ovarian Follicle Development ◽  
Endocrine Status ◽  
Ovulatory Follicles ◽  
Follicle Maturation

It was postulated that the induction of twin ovulations in cattle may require a combination of two appropriate stimuli. The first putative requirement is an increase in the circulating FSH concentrations and/or a decrease in inhibin concentrations to induce the development of additional potentially ovulatory follicles. The second putative requirement is an increase in LH pulse frequency during the follicular phase of the cycle to enhance the stimulus for follicle maturation and ovulation. On the basis of studies reported in the literature, it was suggested that such stimuli may be provided by immunization against inhibin and against a steroid hormone such as androstenedione, respectively. This experiment was designed to determine the effects of immunization against androstenedione and inhibin, either separately or together on the endocrine status of heifers and on the associated patterns of ovarian follicle development and ovulation rate.

207 EFFECT OF LOCATION AND STAGE OF THE DOMINANT FOLLICLE ON OVULATION AND PREGNANCY RATES IN ALPACAS

2010 ◽  
Vol 22 (1) ◽  
pp. 261
Author(s):  
M. Cervantes ◽  
W. Huanca ◽  
A. Cordero ◽  
M. Guerra ◽  
X. P. Valderrama ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Ovarian Follicle ◽  
Ovarian Response ◽  
Pregnancy Rates ◽  
Left Ovary ◽  
Ovulation Rate ◽  
Dominant Follicle ◽  
Embryo Survival ◽  
Ovarian Follicle Development ◽  
Stage Of Development

Gestation in camelids occurs in the left horn regardless of whether ovulation has taken place in the left or right ovary, suggesting uterine embryo migration (Fernandez-Baca S et al. 1970 Biol. Reprod. 3, 243-251). On the other hand, we have previously documented (Ratto MH et al. 2003 Theriogenology 60, 1645-1656) that more than 90% of llamas with the presence of a follicle ≥6 to 7 mm in diameter, regardless of their stage of development, did accept the copula and ovulate after mating. However, it is unknown whether these oocytes are competent to achieving acceptable pregnancy rate. This study was designed to determine the effect of location of the preovulatory dominant follicle (right or left ovary) on ovulation and pregnancy rates and to evaluate the effect of stage of ovarian follicle development at mating (growing, maintenance, and regression) on ovulation rate and embryo survival in alpacas. In Experiment 1, nonlactating alpacas (4-6 years of age) weighing 55 to 75 kg were randomly assigned to 1 of 2 groups according to the location of the dominant follicle detected by ultrasonography: right ovary [right dominant follicle (RDF); n = 96] or left ovary [left dominant follicle (LDF); n = 108]. Ovulation and pregnancy diagnoses were assessed by ultrasonography on Days 2 (Day 0 = mating) and 30, respectively. Ovulation was defined as the sudden disappearance of a large follicle (≥6 mm) that was detected during the ultrasonographic examination 24 to 36 h after mating. Ovulation rates (96.5 and 96.3% for RDF and LDF, respectively) and pregnancy rates (60.2 and 56.7% for RDF and LDF, respectively) rates did not differ (P = 0.9) among groups. In Experiment 2, nonlactating alpacas (4-8 years of age) weighing 60 to 80 kg (n = 4116) were submitted to ultrasound-guided follicle ablation to synchronize follicular wave emergence and, after daily ultrasonography examination, were randomly assigned to the following groups according to the growth phase and diameter of the dominant follicle: early growing (G1; 5-6 mm, n = 27), mid-growing (G2; 7-12 mm, n = 30); static (G3; 7-12 mm, n = 430), or regressing (G4; 12-7 mm, n = 29). All alpacas were mated with a proven male, except 5 alpacas from G1 that rejected the sire. The ovarian response was determined by ultrasound examinations that were carried out on Day 2 (ovulation rate), Day 9 (CL size), and Day 35 (presence of embryonic vesicle or embryo). Ovulation was determined as described in Experiment 1. No differences were detected in ovulation rate among groups (95, 96, 100, and 96%, respectively; P = 0.8) or in CL size (10.3 ± 0.8; 11.7 ± 0.6; 11.1 ± 0.8; and 11.1 ± 0.9 mm, respectively; P = 0.6). Pregnancy rate was highest in G2 (65.5%), intermediate in G1 (52.4%) and G3 (53.3%), and lowest in G4 (42.9%; P < 0.05). Results suggest that location of the dominant follicle has no influence on ovulation and pregnancy rates and that although mating during the regressing phase of the dominant follicle has no effect on ovulation, pregnancy rate may be compromised. This study was supported by DID-UACH, Universidad Austral de Chile.

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