scholarly journals Effect of GnRH conjugated to pokeweed antiviral protein on reproductive function in adult male dogs

Reproduction ◽  
2003 ◽  
pp. 801-806 ◽  
Author(s):  
K Sabeur ◽  
BA Ball ◽  
TM Nett ◽  
HH Ball ◽  
IK Liu
Keyword(s):  
Adult Male ◽  
Area Under The Curve ◽  
Reproductive Function ◽  
Serum Testosterone ◽  
Control Group ◽  
Gnrh Analogue ◽  
Testis Size ◽  
Pokeweed Antiviral Protein ◽  
Antiviral Protein ◽  
Lh Release

This study evaluated the effect of a GnRH analogue conjugated to the cytotoxin, pokeweed antiviral protein (PAP), on reproductive function in adult, male dogs. Four dogs received 0.0042 mg GnRH-PAP kg(-1) hourly for 36 h, and four other dogs received 0.1 mg GnRH-PAP kg(-1) as one bolus injection daily for three consecutive days. One dog received a single bolus (0.1 mg x kg(-1)). Three adult male dogs received GnRH without the PAP conjugate, as controls. Twenty-five weeks after the initial treatment, all treated dogs received 0.1 mg GnRH-PAP kg(-1) as a single administration, whereas dogs in the control group received 0.0045 mg kg(-1) of the GnRH analogue. Serum concentrations of testosterone and LH were determined by radioimmunoassay, and testis size was measured for 9 months after treatment. Stimulation tests (5 microg GnRH kg(-1)) were used to evaluate LH release (-15, 0, 30, 60, 90, 120 min), which was assessed by measuring area under the curve. Serum testosterone concentrations were significantly lower (P<0.05) after treatment in the bolus and hourly groups than in the control group. Testosterone concentrations fell to less than 50 pg x ml(-1) in three of four dogs in the bolus group and one of four dogs in the hourly group by week 8-9 after treatment. Basal LH was lower (P<0.05) in the bolus and hourly groups than in the control group between weeks 0 and 33 after treatment. Treatment with GnRH-PAP reduced (P<0.05) LH release after GnRH stimulation in the bolus and hourly groups compared with the control group. Testis volume was lower (P<0.05) in all treated versus control dogs. In conclusion, administration of the conjugate GnRH-PAP at a 25 week interval resulted in a major disruption of reproductive parameters in male dogs; this effect was maintained for 11-12 weeks after a second injection of GnRH-PAP.

scholarly journals The Effect of Kuchala (Arum Korolkowii Regel, 1873) Tuber Tincture to Increase of the Serum Testosterone in the Adult Male Guinea Pigs (Cavia Porcellus Linnaeus, 1758)

2020 ◽  
Author(s):  
NURBEK SAIDILLAEVICH ALDAYROV ◽  
JARKINAY JUMABEKOVA ◽  
GULBUBU KURMANBEKOVA ◽  
NURJAMAL OMURZAKOVA ◽  
BERMET KIDIRALIEVA ◽  
...  
Keyword(s):  
Adult Male ◽  
Guinea Pigs ◽  
Structural Damage ◽  
Folk Medicine ◽  
Serum Testosterone ◽  
Serum Biochemistry ◽  
Raw Material ◽  
Control Group ◽  
Kyrgyz Republic ◽  
Experimental Group

Abstract BackgroundKuchala (Arum korolkowii Regel) is one of the medicinal plants often used in folk medicine in the Kyrgyz Republic. As a medicinal raw material, tubers tincture used in small doses to increase human sexual potency. However, there is no scientific evidence proving the medicinal effects of kuchala. For these reasons, we decided to study the effect of kuchala tuber tincture on the sexual potency of adult male guinea pigs.MethodsWe analyzed the effect of kuchala at age ±48-month-old, in 12 male guinea pigs. Prepared 10% tuber tincture of kuchala in 70% ethanol was administered perorally a once-daily dose of 150 µl for 30 days for the male guinea pigs. These study data were obtained by the ethological, hematological and serum biochemistry, gross anatomical, histological and statistical methods.ResultsThe hematological and serum biochemistry parameters were significantly different between control and experimental group. Neutrophils’ percentage in experimental group was significantly lower (dР < 0.001) than in control group. Lymphocyte counts on the contrary were significantly higher in experimental group (dР < 0.001). RBC counts, Hgb, Hct, MCH and MCHC were significantly higher in the experimental group (dР < 0.001; dР < 0.001; cР < 0.01; dР < 0.001; dР < 0.001 respectively) than control group. The color indicator and mean platelet volume contrary was higher (bР < 0.05) and significantly higher (dР < 0.001) in control animals than in the experimental. ALT and AST levels were lower in experimental than control group (both dР < 0,001). The testosterone concentration in serum was much higher (dР < 0,001) in the experimental group. Microscopically was found minor structural damage in the liver tissue that indicates a metabolic disorder. However, the testes showed an improvement in spermatogenesis in the experimental if compared with the control group.ConclusionThe 10% kuchala tuber tincture in 70% ethanol has a positive effect to improve the sexual potency of old guinea pigs by increasing the production of testosterone and increasing spermatogenesis.

scholarly journals Complementary effects of coenzyme Q10 and Lepidium sativum supplementation on the reproductive function of mice: An experimental study

2021 ◽  
Author(s):  
Fatemeh Rahimi Asl ◽  
Maryam Khosravi ◽  
Ramin Hajikhani ◽  
Jalal Solati ◽  
Hossein Fahimi
Keyword(s):  
Body Weight ◽  
Luteinizing Hormone ◽  
Coenzyme Q10 ◽  
Follicle Stimulating Hormone ◽  
Reproductive Function ◽  
Serum Testosterone ◽  
Lepidium Sativum ◽  
Control Group ◽  
Male Mice ◽  
Weight Groups

Background: Coenzyme Q10 (CoQ10) and Lepidium sativum (LS) have therapeutic effects on infertility. Objective: To evaluate the combined effects of LS and CoQ10 on reproductive function in adult male NMRI mice. Materials and Methods: Eighty three-months-old male mice (35–40 gr) were divided into four groups (n = 10/each): control (treated with water), CoQ10-treated (200, 300, and 400 mg/kg/body weight), LS-treated (200, 400, 600 mg/kg/body weight), and co-treated (LS [600 mg/kg/body weight] + CoQ10 [200 mg/kg/body weight]) groups. Serum testosterone, luteinizing hormone, follicle-stimulating hormone, and gonadotropin realizing hormone (GnRH) levels were measured using ELISA method. The sperm quality was assessed using Sperm Class Analyzer® (SCA) CASA system and GnRH mRNA expression levels were evaluated by real-time polymerase chain reaction. Results: The number of sniffing and following behavior was significantly higher in LStreated (400 and 600 mg/ml/body weight) groups than the control group (p = 0.0007 and p = 0.0010, respectively). The number of mounting and coupling behaviors was significantly higher in the CoQ10 (300 and 400 mg/ml/body weight)-treated animals than the control group (p = 0.0170 and p = 0.0006, respectively). Co-treatment of CoQ10 (200 mg/ml/body weight) and LS (600 mg/ml/body weight) significantly increased all aspects of sexual behaviors as well as the levels of serum testosterone (p = 0.0011), luteinizing hormone (p = 0.0062), and follicle-stimulating hormone (p = 0.0001); sperm viability (p = 0.0300) and motility (p = 0.0010); and GnRH mRNA levels (p = 0.0016) compared to the control group. Conclusion: The coadministration of CoQ10 and LS significantly improves the activity of the hypothalamic-pituitary-gonadal axis and enhances the reproductive parameters in adult male mice. Key words: Lepidium sativum, Coenzyme Q10, Infertility, Male reproductive function.

scholarly journals The Effect of Kuchala (Arum Korolkowii Regel, 1873) Tuber Tincture to Increase of the Serum Testosterone in the Adult Male Guinea Pigs (Cavia Porcellus Linnaeus, 1758)

2021 ◽  
Author(s):  
Nurbek Aldayarov ◽  
Jarkinay Jumabekova ◽  
Gulbubu Kurmanbekova ◽  
Nurjamal Omurzakova ◽  
Bermet Kydyralieva ◽  
...  
Keyword(s):  
Adult Male ◽  
Guinea Pigs ◽  
Scientific Evidence ◽  
Folk Medicine ◽  
Serum Testosterone ◽  
Serum Biochemistry ◽  
Raw Material ◽  
Control Group ◽  
Kyrgyz Republic ◽  
Experimental Group

Abstract Background: Kuchala (Arum korolkowii Regel) is a medicinal plant often used in folk medicine in the Kyrgyz Republic. As a medicinal raw material, the tuber’s tincture is used in small doses to increase human sexual potency. However, there is no scientific evidence in support of the medicinal effects of kuchala. For these reasons, we decided to study the pharmacological effect of kuchala tuber tincture on the sexual potency of adult male guinea pigs. We analyzed the effect of kuchala at the age of ±48-months, in 12 male guinea pigs. A preparation of 10% tuber tincture of kuchala in 70% ethanol was administered perorally to the male guinea pigs in the form of a once-daily dose of 150 µl for 30 days. The study data were obtained by ethological, hematological and serum biochemistry, gross anatomical, histological and statistical methods.Results: The hematological and serum biochemistry parameters were significantly different between the control and the experimental group. The neutrophils’ percentage in the experimental group was significantly lower (dР < 0.001) than in the control group. On the other hand, the lymphocyte counts were significantly higher in the experimental group (dР < 0.001). The RBC counts, Hgb, Hct, MCH and MCHC were significantly higher in the experimental group (dР < 0.001; dР < 0.001; cР < 0.01; dР < 0.001; dР < 0.001 respectively) than in the control group. In contrast, the color indicator and the mean platelet volume were higher (bР < 0.05) and significantly higher (dР < 0.001) respectively in the control group than in the experimental group. The ALT and AST levels were lower in the experimental than in the control group (both dР < 0,001). The testosterone concentration in serum was much higher (dР < 0,001) in the experimental group. Microscopically, some structural damages were found in the liver of the experimental animals indicating a metabolic disorder. However, the testes showed an improvement in spermatogenesis in the experimental compared with the control group.Conclusions: The 10% kuchala tuber tincture in 70% ethanol has a positive effect in terms of improving the sexual potency of adult guinea pigs by increasing the production of testosterone and increasing spermatogenesis.

scholarly journals Fertility and mortality impacts of thermal stress from experimental heatwaves on different life stages and their recovery in a model insect

2021 ◽  
Vol 8 (3) ◽  
Author(s):  
Kris Sales ◽  
Ramakrishnan Vasudeva ◽  
Matthew J. G. Gage
Keyword(s):  
Heat Stress ◽  
Thermal Stress ◽  
High Temperatures ◽  
Adult Male ◽  
Reproductive Function ◽  
Testis Size ◽  
Life Stages ◽  
Mature Adult ◽  
Male Reproductive Function ◽  
Survival And Reproduction

With climate change creating a more volatile atmosphere, heatwaves that create thermal stress for living systems will become stronger and more frequent. Using the flour beetle Tribolium castaneum , we measure the impacts of thermal stress from experimental heatwaves in the laboratory on reproduction and survival across different insect life stages, and the extent and pace of any recovery. We exposed larvae, pupae, juvenile and mature adult male beetles to 5-day periods of heat stress where temperatures were maintained at either 40°C or 42°C, a few degrees above the 35°C optimum for this species' population productivity, and then measured survival and reproduction compared with controls at 30°C. Mortality due to thermal stress was greatest among juvenile life stages. Male reproductive function was specifically damaged by high temperatures, especially if experienced through pupal or immature life stages when complete sterility was shown at reproductive maturity; larval exposure did not damage adult male fertility. High temperatures impaired testis development and the production of viable sperm, with damage being strongest when experienced during pupal or juvenile adult stages. Despite this disruption, males recovered from heat stress and, depending on the stage of exposure, testis size, sperm production and fertility returned to normal 15–28 days after exposure. Our experiments reveal how thermal stress from heatwave conditions could impact on insect survival and reproduction across different life stages, and the potential and timescales of recovery.

scholarly journals Leptin inhibits testosterone secretion from adult rat testis in vitro

1999 ◽  
Vol 161 (2) ◽  
pp. 211-218 ◽  
Author(s):  
M Tena-Sempere ◽  
L Pinilla ◽  
LC Gonzalez ◽  
C Dieguez ◽  
FF Casanueva ◽  
...  
Keyword(s):  
Adult Male ◽  
Reproductive Function ◽  
Serum Testosterone ◽  
Testicular Tissue ◽  
Male Rats ◽  
Adult Males ◽  
Adult Rats ◽  
Adult Rat ◽  
Testosterone Secretion

Leptin, the product of the ob gene, has emerged recently as a pivotal signal in the regulation of fertility. Although the actions of leptin in the control of reproductive function are thought to be exerted mainly at the hypothalamic level, the potential direct effects of leptin at the pituitary and gonadal level have been poorly characterised. In the present study, we first assessed the ability of leptin to regulate testicular testosterone secretion in vitro. Secondly, we aimed to evaluate whether leptin can modulate basal gonadotrophin and prolactin (PRL) release by incubated hemi-pituitaries from fasted male rats. To attain the first goal, testicular slices from prepubertal and adult rats were incubated with increasing concentrations (10(-9)-10(-7) M) of recombinant leptin. Assuming that in vitro testicular responsiveness to leptin may be dependent on the background leptin levels, testicular tissue from both food-deprived and normally-fed animals was used. Furthermore, leptin modulation of stimulated testosterone secretion was evaluated by incubation of testicular samples with different doses of leptin in the presence of 10 IU human chorionic gonadotrophin (hCG). In addition, analysis of leptin actions on pituitary function was carried out using hemi-pituitaries from fasted adult male rats incubated in the presence of increasing concentrations (10(-9)-10(-7) M) of recombinant leptin. Serum testosterone levels, and basal and hCG-stimulated testosterone secretion by incubated testicular tissue were significantly decreased by fasting in prepubertal and adult male rats. However, a significant reduction in circulating LH levels was only evident in adult fasted rats. Doses of 10(-9)-10(-7) M leptin had no effect on basal or hCG-stimulated testosterone secretion by testes from prepubertal rats, regardless of the nutritional state of the donor animal. In contrast, leptin significantly decreased basal and hCG-induced testosterone secretion by testes from fasted and fed adult rats. In addition, 10(-9) M leptin inhibited LH and FSH secretion by incubated hemi-pituitaries from fasted adult males, whereas, at all doses tested, it was ineffective in modulating PRL release. Our results show that leptin, depending on the state of sexual maturation, is able to inhibit testosterone secretion acting at the testicular level. Furthermore, the present data suggest that the actions of leptin on the reproductive system are complex and are probably carried out at different levels of the hypothalamic-pituitary-gonadal axis.

The role of sex hormones in the mechanism of inhibited LH release in female patients with anorexia nervosa

1982 ◽  
Vol 99 (3) ◽  
pp. 334-338 ◽  
Author(s):  
B. Baranowska ◽  
S. Zgliczyński
Keyword(s):  
Anorexia Nervosa ◽  
Serum Testosterone ◽  
Sex Hormone Binding Globulin ◽  
Control Group ◽  
Hormonal Changes ◽  
Female Patients ◽  
Serum Lh ◽  
Before And After ◽  
Lh Release ◽  
Lh Secretion

Abstract. In order to elucidate the mechanism of impaired LH secretion, 60 female patients with anorexia nervosa were investigated. A control group consisted of 14 women of the same age, examined in the follicular phase of the menstrual cycle. The serum LH, FSH, prolactin, TSH, testosterone, dihydrotestosterone, dehydroepiandrosterone, Δ 4-androstenedione, oestrone, oestradiol, oestriol, progesterone, thyroxine, triiodothyronine, reverse T3 and serum sex hormone binding globulin (SHBG) concentrations were measured. The results showed a significant increase in serum dehydroepiandrosterone, testosterone, oestriol and reverse T3 concentrations. However, oestrone, oestradiol, progesterone, SHBG and triiodothyronine levels were significantly lower than those of the control group. The mean serum LH concentration in patients with anorexia nervosa before and after LRH stimulation was significantly lower than that in the control group, but FSH secretion in response to LRH was normal. All hormonal changes in anorexia nervosa disappeared after weight gain during cyproheptadine treatment. Dramatically increased dehydroepiandrosterone levels suggest that the high testosterone in women with anorexia nervosa is derived from adrenal rather than from gonadal steroids. There was no correlation between serum testosterone, dehydroepiandrosterone, oestriol and LH concentrations indicating that steroid hormone disturbances do not cause impaired LH release in anorexia nervosa.

scholarly journals Metabolism of Orally Administered Androstenedione in Young Men

2001 ◽  
Vol 86 (8) ◽  
pp. 3654-3658 ◽  
Author(s):  
Benjamin Z. Leder ◽  
Don H. Catlin ◽  
Christopher Longcope ◽  
Brian Ahrens ◽  
David A. Schoenfeld ◽  
...  
Keyword(s):  
Urinary Excretion ◽  
General Circulation ◽  
Area Under The Curve ◽  
Serum Testosterone ◽  
Free Testosterone ◽  
Serum Levels ◽  
Blood Sampling ◽  
Control Group ◽  
Total Testosterone ◽  
Excretion Rates

Androstenedione is a steroid hormone and the major precursor to testosterone. It is available without prescription and taken with the expectation that it will be converted to testosterone endogenously and increase strength and athletic performance. The metabolism of orally administered testosterone has not been well studied. We randomly assigned 37 healthy men to receive 0, 100, or 300 mg oral androstenedione in a single daily dose for 7 d. Single 8-h urine collections were performed on the day before the start of the androstenedione administration and on d 1 and 7 to assess excretion rates of free and glucuronide- conjugated testosterone, androsterone, etiocholanolone, and dihydrotestosterone. Serum testosterone glucuronide concentrations were measured by frequent blood sampling over 8 h on d 1 in 16 subjects (5 each in the 0 and 100 mg group and 6 in the 300 mg group). In the control group, mean (±se) d 1 and 7 excretion rates for testosterone, androsterone, etiocholanolone, and dihydrotestosterone were 3 ± 1, 215 ± 26, 175 ± 26, and 0.4 ± 0.1 μg/h, respectively. In the 100 mg group, mean d 1 and 7 excretion rates for testosterone, androsterone, etiocholanolone, and dihydrotestosterone were 47 ± 11, 3,836 ± 458, 4,306 ± 458, and 1.6 ± 0.2 μg/h, respectively. In the 300 mg group, mean d 1 and 7 excretion rates for testosterone, androsterone, etiocholanolone, and dihydrotestosterone were 115 ± 39, 8,142 ± 1,362, 10,070 ± 1,999, and 7.7 ± 1.5μ g/h, respectively. Urinary excretion rates of all metabolites were greater in both the 100 and 300 mg groups than in controls (P &lt; 0.0001). Urinary excretion rates of testosterone (P = 0.007), androsterone (P = 0.009), etiocholanolone (P= 0.0005), and dihydrotestosterone (P &lt; 0.0001) were greater in the subjects who received 300 mg androstenedione than in those who received 100 mg. In the treated groups, excretion of free testosterone accounted for less than 0.1% of the total excreted testosterone measured. Serum testosterone glucuronide levels increased significantly during frequent blood sampling in both the 100 and 300 mg groups compared with controls (P = 0.0005 for the 100 mg group; P &lt; 0.0001 for the 300 mg group). The net mean changes in area under the curve for serum testosterone glucuronide were −18 ± 25%, 579 ± 572%, and 1267 ± 1675% in the groups receiving 0, 100, and 300 mg/d androstenedione, respectively. We conclude that the administration of both 100 and 300 mg androstenedione increases the excretion rates of conjugated testosterone, androsterone, etiocholanolone, and dihydrotestosterone and the serum levels of testosterone glucuronide in men. The magnitude of these increases is much greater than the changes observed in serum total testosterone concentrations. These findings demonstrate that orally administered androstenedione is largely metabolized to testosterone glucuronide and other androgen metabolites before release into the general circulation.

scholarly journals Effects of a Recombinant Gonadotropin-Releasing Hormone Vaccine on Reproductive Function in Adult Male ICR Mice

Vaccines ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 808
Author(s):  
Ai-Mei Chang ◽  
Chen-Chih Chen ◽  
Ding-Liang Hou ◽  
Guan-Ming Ke ◽  
Jai-Wei Lee
Keyword(s):  
Adult Male ◽  
Semen Quality ◽  
Sperm Quality ◽  
Endocrine System ◽  
Reproductive Function ◽  
Gonadotropin Releasing Hormone ◽  
Testicular Atrophy ◽  
Testis Size ◽  
Releasing Hormone ◽  
Icr Mice

Gonadotropin-releasing hormone (GnRH) regulates the reproductive endocrine system in mammals. The GnRH immunocontraception vaccine can aid animal population control and management. We evaluated a recombinant GnRH fusion protein with the adjuvant MONTANIDE ISA 206 VG as a GnRH vaccine in adult male ICR mice by evaluating anti-GnRH antibodies; concentrations of follicle-stimulating hormone (FSH), luteinizing hormone (LH), and testosterone; testis size and histomorphology; and semen quality. Response was assessed after intramuscular administration of the vaccine to mice in weeks 0, 4, and 8. The vaccine induced specific antibody response by week 5, with peak of antibody levels observed by week 13 and a declining level thereafter until the end of the study at week 24. Furthermore, it reduced serum FSH, LH, and testosterone concentrations. The vaccinated mice exhibited testicular atrophy and reduced sperm quality, concentration, morphology, and viability compared to control males. The outcomes of pairings of treated males with untreated females revealed reduced mating, pregnancy rates and number of litters compared to control pairings. Assessment of this GnRH vaccine in different species could assist its development for future applications.

Protective effect of green tea extract against cadmium-induced testicular damage in rats in respect of oxidant/antioxidant equilibrium and androgen production

2020 ◽  
Vol 21 (1) ◽  
pp. 31-35
Author(s):  
Basma El-Desoky ◽  
Shaimaa El-Sayed ◽  
El-Said El-Said
Keyword(s):  
Gene Expression ◽  
Single Dose ◽  
Green Tea ◽  
Serum Testosterone ◽  
Green Tea Extract ◽  
Male Rats ◽  
Controlled Study ◽  
Control Group ◽  
Testicular Damage ◽  
Tea Extract

Objective: Investigating the effect of green tea extract (GTE) on the testicular damage induced by cadmium chloride CdCl2 in male rats. Design: Randomized controlled study. Animals: 40 male Wistar rats. Procedures: Rats were randomly divided into four groups: A) control group (each rat daily received pellet diet); B) GTE group each rat daily received pellet diet as well as 3 ml of 1.5 % w/v GTE, C) CdCl2 group each rat was I/P injected a single dose of 1 mg/kg CdCl2, then daily received pellet diet, and D) CdCl2+GTE group each rat was I/P injected a single dose of 1 mg/kg CdCl2 then daily received pellet diet as well as 3 ml of 1.5 % w/v GTE. After 30 days, blood samples were collected for hormonal assays (testosterone, FSH, and LH). In addition, both testes were collected; one of them was used for quantification of 17-beta hydroxysteroid dehydrogenase III (17β-HSDIII) gene expression using a real-time PCR. The other testis was used for determination of catalase and reduced glutathione; GSH, Nitric oxide (NO) and malondialdehyde (MDA) levels. Results: CdCl2 decreased serum testosterone levels and its synthesis pathway (17β-HSDIII testicular gene expression). While antioxidants catalase and GSH were reduced, oxidants MDA were enriched in the testes of CdCl2-poisoned rats. This CdCl2-promoted testicular dysfunction was corrected via the administration of GTE to male rats. Conclusion and clinical relevance: GTE could be used as a remedy for protecting against CdCl2-induced testicular damage in male rats.

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