scholarly journals Segregation of spermatozoa within sperm storage tubules of fowl and turkey hens

Reproduction ◽  
2002 ◽  
pp. 79-86 ◽  
Author(s):  
LM King ◽  
JP Brillard ◽  
WM Garrett ◽  
MR Bakst ◽  
AM Donoghue
Keyword(s):  
Sperm Motility ◽  
Fluorescent Dyes ◽  
Sperm Storage ◽  
Relative Distribution ◽  
Hoechst 33342 ◽  
Sperm Entry ◽  
Sperm Nuclei ◽  
Sperm Storage Tubules ◽  
And Storage ◽  
Mammalian Spermatozoa

In avian species, spermatozoa reside in the oviduct for prolonged periods in specialized structures known as sperm storage tubules, but little is known about the relative distribution of spermatozoa in these tubules after successive inseminations by different males. The staining efficacies of various fluorescent dyes for fowl and turkey spermatozoa were evaluated to investigate one proposed mechanism of sperm competition. Hens were then inseminated at different intervals with stained and unstained spermatozoa to observe the spatial distribution of spermatozoa within the storage tubules. Several novel fluorescent lipophilic tracers that successfully stain mammalian spermatozoa either did not stain fowl or turkey spermatozoa, or greatly impaired sperm motility. In contrast, Hoechst 33342 readily stained sperm nuclei (fowl: 25 nmol l-1; turkey: 77 nmol l-1) within 4 h without inhibiting sperm motility, or affecting fertility or the hatching ability of the eggs. Hens were tandemly inseminated with equal numbers of stained or unstained spermatozoa at 24 h intervals and were killed 24 h after the final insemination to study sperm entry and storage within the tubules. Oviductal mucosa containing sperm storage tubules was removed, and individual tubules were classified as containing stained spermatozoa, unstained spermatozoa, a mixture of stained and unstained spermatozoa, or as not containing spermatozoa. Results from the present study indicate that spermatozoa from two different inseminations generally segregate into different storage tubules in both fowl and turkey hens. Storage tubules containing mixed populations of spermatozoa were found in only 4% of fowl and 12% of turkey storage tubules examined. Thus, the mechanism of last-male precedence does not appear to be due to the stratification of spermatozoa within the tubules.

scholarly journals Sperm activation by heat shock protein 70 supports the migration of sperm released from sperm storage tubules in Japanese quail (Coturnix japonica)

Reproduction ◽  
2014 ◽  
Vol 147 (2) ◽  
pp. 167-178 ◽  
Author(s):  
Gen Hiyama ◽  
Mei Matsuzaki ◽  
Shusei Mizushima ◽  
Hideo Dohra ◽  
Keisuke Ikegami ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Sperm Motility ◽  
Heat Shock Protein 70 ◽  
Sperm Storage ◽  
Female Reproductive Tract ◽  
Voltage Dependent Anion Channel ◽  
Sperm Surface ◽  
Recombinant Hsp70 ◽  
Sperm Storage Tubules

Systems for maintaining the viability of ejaculated sperm in the female reproductive tract are widespread among vertebrates and invertebrates. In birds, this sperm storage function is performed by specialized simple tubular invaginations called sperm storage tubules (SSTs) in the uterovaginal junction (UVJ) of the oviduct. Although the incidence and physiological reasons for sperm storage in birds have been reported extensively, the mechanisms of sperm uptake by the SSTs, sperm maintenance within the SSTs, and control of sperm release from the SSTs are poorly understood. In this study, we demonstrated that the highly conserved heat shock protein 70 (HSP70) stimulates sperm motility in vitro and also that HSP70 expressed in the UVJ may facilitate the migration of sperm released from the SSTs. Quantitative RT-PCR analysis demonstrated that the expression of HSP70 mRNA in the UVJ increases before ovulation/oviposition. Gene-specific in situ hybridization and immunohistochemical analysis with a specific antibody to HSP70 demonstrated that HSP70 is localized in the surface epithelium of the UVJ. Furthermore, injection of anti-HSP70 antibody into the vagina significantly inhibited fertilization in vivo. In addition, we found that recombinant HSP70 activates flagellar movement in the sperm and that the binding of recombinant HSP70 to the sperm surface is mediated through an interaction with voltage-dependent anion channel protein 2 (VDAC2). Our results suggest that HSP70 binds to the sperm surface by interacting with VDAC2 and activating sperm motility. This binding appears to play an important role in sperm migration within the oviduct.

scholarly journals Lactic acid is a sperm motility inactivation factor in the sperm storage tubules

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Mei Matsuzaki ◽  
Shusei Mizushima ◽  
Gen Hiyama ◽  
Noritaka Hirohashi ◽  
Kogiku Shiba ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Sperm Motility ◽  
Sperm Storage ◽  
Sperm Storage Tubules

Localization of calcium and zinc in the sperm storage tubules of chicken, quail and turkey using X-ray microanalysis

Reproduction ◽  
2000 ◽  
pp. 331-336 ◽  
Author(s):  
L Holm ◽  
H Ekwall ◽  
GJ Wishart ◽  
Y Ridderstrale
Keyword(s):  
Calcium Concentration ◽  
Sperm Storage ◽  
X Ray ◽  
Elemental Concentrations ◽  
Low Concentrations ◽  
Intracellular Content ◽  
Wet Weight ◽  
The Mean ◽  
Sperm Storage Tubules ◽  
Scanning Electron

Sperm storage tubules from the utero-vaginal junction of chickens, quails and turkeys were analysed for calcium and zinc using X-ray microanalysis of ultra-rapidly frozen tissue in a scanning electron microscope. This technique enabled the tubular fluid surrounding the stored spermatozoa and the intracellular content of the cells of the sperm storage tubules to be analysed separately and, by using standards with known concentrations, their elemental concentrations were estimated. The mean (+/- SEM) concentration of calcium in the tubular fluid from chickens, quails and turkeys was 17 +/- 3, 19 +/- 3 and 17 +/- 4 mmol kg(-1) wet weight, respectively. The intracellular calcium concentration of the cells of the tubules did not differ significantly from these values and was also similar in the mucosal epithelial cells of the utero-vaginal junction. Zinc was localized in the cells of turkey sperm storage tubules and tubular fluid, but at low concentrations. No zinc could be detected in corresponding structures from chickens and quails. The concentration of calcium in the tubular fluid is within the range known to inhibit the motility of spermatozoa, supporting this function for calcium during storage. Zinc is known to depress turkey sperm metabolism and it may also be involved in inducing quiescence of spermatozoa during storage in this species.

scholarly journals Expression of Mst89B and CG31287 is Needed for Effective Sperm Storage and Egg Fertilization in Drosophila

Cells ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 289
Author(s):  
Gurman Grewal ◽  
Bahar Patlar ◽  
Alberto Civetta
Keyword(s):  
Germ Line ◽  
Sperm Storage ◽  
Mature Sperm ◽  
Sperm Production ◽  
Cytogenetic Map ◽  
Egg Hatchability ◽  
Storage Organs ◽  
Competitive Success ◽  
Paternity Success ◽  
And Storage

In Drosophila, male reproductive fitness can be affected by any number of processes, ranging from development of gametes, transfer to and storage of mature sperm within the female sperm storage organs, and utilization of sperm for fertilization. We have previously identified the 89B cytogenetic map position of D. melanogaster as a hub for genes that effect male paternity success when disturbed. Here, we used RNA interference to test 11 genes that are highly expressed in the testes and located within the 89B region for their role in sperm competition and male fecundity when their expression is perturbed. Testes-specific knockdown (KD) of bor and CSN5 resulted in complete sterility, whereas KD of CG31287, Manf and Mst89B, showed a breakdown in sperm competitive success when second to mate (P2 < 0.5) and reduced fecundity in single matings. The low fecundity of Manf KD is explained by a significant reduction in the amount of mature sperm produced. KD of Mst89B and CG31287 does not affect sperm production, sperm transfer into the female bursa or storage within 30 min after mating. Instead, a significant reduction of sperm in female storage is observed 24 h after mating. Egg hatchability 24 h after mating is also drastically reduced for females mated to Mst89B or CG31287 KD males, and this reduction parallels the decrease in fecundity. We show that normal germ-line expression of Mst89B and CG31287 is needed for effective sperm usage and egg fertilization.

Localization of calcium and zinc in the sperm storage tubules of chicken, quail and turkey using X-ray microanalysis

Reproduction ◽  
2000 ◽  
Vol 118 (2) ◽  
pp. 331-336 ◽  
Author(s):  
L Holm ◽  
H Ekwall ◽  
G. Wishart ◽  
Y Ridderstrale
Keyword(s):  
Sperm Storage ◽  
X Ray ◽  
Sperm Storage Tubules

scholarly journals Seminal and spermatic characteristics of fresh semen and the effects of sperm cooling in Steindachneridion melanodermatum (Garavello, 2005)

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4493
Author(s):  
Ronan Maciel Marcos ◽  
Giovano Neumann ◽  
Cesar Pereira Rebechi de Toledo ◽  
João Marcos Sena ◽  
Gilmar Baumgartner ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Storage Temperature ◽  
Sperm Concentration ◽  
Plasma Osmolality ◽  
Storage Period ◽  
Sperm Velocity ◽  
Powdered Milk ◽  
Sperm Activation ◽  
And Storage ◽  
Fresh Semen

<p>This study describes the seminal and spermatic characteristics of fresh semen of <em>Steindachneridion melanodermatum </em>and investigates the effects of dilution, temperature, and storage period on its spermatic parameters. Sperm samples were collected from nine hormonally-induced males. The following parameters in fresh sperm were analyzed: seminal plasma osmolality (OSM), seminal pH, sperm motility (MOT), sperm velocity (SV) (including sperm curvilinear velocity (CVV), sperm straight-line velocity (SLV), and sperm average path velocity (APV)), total time of sperm motility (TEMP), sperm concentration (CONC), and index of sperm normality (NORM). Sperm samples from each male were diluted in a solution containing 5% fructose and 5% powdered milk, and stored at 10°C and 25°C. The same was carried out for sperm samples not subjected to dilution. From these samples, MOT, CVV, SLV, APV, SV, and TEMP were measured after 0 h, 5 h, 9 h, 18 h, 27 h, 36 h, 45 h, and 54 h. Males released 11.74 ± 5.38 mL of sperm, with an osmolality of 258.78 ± 29.36 mOsm.kg-1 and pH of 7.11 ± 0.31. The sperm presented a MOT of 99.86 ± 0.31% at a concentration of 1.03 × 1010 ± 3.65 × 109 spermatozoa.mL-1 with CVV of 185.58 ± 14.11 ?m.s-1, SLV of 49.15 ± 4.66 ?m.s-1, APV of 87.02 ± 4.13 ?m.s-1, SV of 106.52 ± 4.45 ?m.s-1, TEMP of 79.31 ± 5.62 s, NORM of 75.81 ± 5.71%. The results indicate that sperm motility, sperm velocity, and total time of sperm activation were affected by dilution, storage temperature, and storage period (p &lt; 0.05). Procedures for semen storage should be performed with undiluted sperm cooled at 10°C, or kept undiluted at 25°C for up to 27 h.</p>

Solar and Storage Degradations of Oil- and Water-Soluble Fluorescent Dyes

2011 ◽  
Vol 27 (2) ◽  
pp. 211-216 ◽  
Author(s):  
L. R. Khot ◽  
M. Salyani ◽  
R. D. Sweeb
Keyword(s):  
Fluorescent Dyes ◽  
Water Soluble ◽  
And Storage
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