scholarly journals Immunohistochemical study of the distribution of adrenergic and peptidergic innervation in the equine uterus and the cervix

Reproduction ◽  
2001 ◽  
pp. 275-282 ◽  
Author(s):  
SE Bae ◽  
BM Corcoran ◽  
ED Watson
Keyword(s):  
Blood Vessels ◽  
Uterine Horn ◽  
Adrenergic Nerves ◽  
Adrenergic Nerve ◽  
Uterine Contractility ◽  
Nerve Fibres ◽  
Peptidergic Nerves ◽  
Dopamine Beta Hydroxylase ◽  
Calcitonin Gene ◽  
Nerve Bundles

Little is known about neurogenic regulation of uterine contractility in mares. The present study investigated the distribution of adrenergic and peptidergic nerves in the mare uterus. Samples from the uterine horn, body and cervix were collected from 18 cyclic mares for immunohistochemistry. The uterus was well supplied with adrenergic nerves. A large number of tyrosine hydroxylase- and dopamine beta-hydroxylase-immunoreactive nerve bundles and fibres were present in the myometrium and endometrium in all regions of the uterus and cervix. These adrenergic nerve bundles and fibres travelled parallel to the muscle layers and were often associated with blood vessels. The density of peptidergic nerves was less than that of adrenergic nerves, but the pattern of distribution was similar. Neuropeptide Y-immunoreactive nerve fibres were the most abundant, whereas vasoactive intestinal polypeptide- and calcitonin gene-related peptide-immunoreactive nerve fibres were less frequently seen. Substance P-immunoreactive nerve fibres were the most sparse. Peptidergic nerves were distributed among the smooth muscle layers and near endometrial glands and were often associated with blood vessels in all regions of the uterus. The density of peptidergic nerve fibres was similar in the uterine horn and body but was slightly denser in the cervix. These findings indicate that uterine innervation may have an important role in controlling reproductive functions in mares.

Platelet 5-Hydroxytryptamine and Vascular Adrenergic Nerves

Physiology ◽  
1988 ◽  
Vol 3 (5) ◽  
pp. 185-189
Author(s):  
RA Cohen
Keyword(s):  
Platelet Aggregation ◽  
Blood Vessels ◽  
Sympathetic Nerves ◽  
Adrenergic Nerves ◽  
Adrenergic Nerve ◽  
Norepinephrine Release ◽  
Neuronal Function ◽  
Uptake Mechanism ◽  
Low Concentrations ◽  
Adrenergic Nerve Endings

Platelets contain and release large amounts of 5-hydroxytryptamine that can influence the function of vascular adrenergic nerves. The immediate effect on neuronal function of low concentrations of the amine is to inhibit norepinephrine release. 5-Hydroxytryptamine may also be accumulated by the same uptake mechanism that reaccumulates norepinephrine into the adrenergic nerve endings. In diseased blood vessels where platelet aggregation occurs, 5-hydroxytryptamine may therby assume an alternative transmitter role, altering the function of the sympathetic nerves.

Peptide-containing nerve fibres in normal human parathyroid glands and in human parathyroid adenomas

1995 ◽  
Vol 133 (5) ◽  
pp. 543-551 ◽  
Author(s):  
Lena Luts ◽  
Anders Bergenfelz ◽  
Jan Alumets ◽  
Frank Sundler
Keyword(s):  
Parathyroid Glands ◽  
Adrenergic Nerve ◽  
Nerve Fibres ◽  
Pgp 9.5 ◽  
Neuronal Markers ◽  
Protein Gene Product ◽  
Calcitonin Gene ◽  
Parathyroid Adenomas ◽  
Pituitary Adenylate Cyclase ◽  
Normal Human

Luts L, Bergenfelz A, Alumets J, Sundler F. Peptide-containing nerve fibres in normal human parathyroid glands and in human parathyroid adenomas. Eur J Endocrinol 1995;133:543–51. ISSN 0804–4643 There are only a few studies on the innervation of the human parathyroid glands and the content of neurotransmitters. We therefore studied the occurrence and distribution of peptide-containing and adrenergic nerve fibres and the coexistence pattern of neuromessengers by immunocytochemistry in normal (unaffected) and adenomatous parathyroid glands from patients undergoing surgery for parathyroid adenoma. The unaffected parathyroid glands had a moderate-to-rich supply of nerve fibres and terminals containing two general neuronal markers, protein gene product 9.5 (PGP 9.5) and synaptophysin, neuropeptide Y (NPY) and tyrosine hydroxylase (TH). They were seen close to blood vessels and, occasionally, among the endocrine cells. Only a few nerves contained calcitonin gene-related peptide (CGRP), vasoactive intestinal polypeptide (VIP), substance P (SP) and pituitary adenylate cyclase-activating peptide (PACAP). The general density of innervation, using PGP 9.5 and synaptophysin as markers, varied greatly among the different adenomas examined. This applied also to the density of fibres and terminals containing specific types of messengers. Some of the tumours had a rich supply of TH- and NPY-containing nerve fibres, while others contained only few scattered fibres. The CGRP-containing fibres varied from moderate in number to no detectable fibres. The PACAP-, SPand VIP-containing fibres were always very few or not detectable. It is not inconceivable that the wide variation in general density of the innervation and frequency of peptide-containing nerves among individual parathyroid adenomas is of significance for their hormone secretory behaviour. Lena Luts, Department of Medical Cell Research, Biskopsgatan 5, S-223 62 Lund, Sweden

Distribution of SP- and CGRP-immunoreactivity in the cat's larynx

1993 ◽  
Vol 107 (6) ◽  
pp. 522-526 ◽  
Author(s):  
Yasumasa Tanaka ◽  
Yoshikazu Yoshida ◽  
Minoru Hirano ◽  
Masatoshi Morimoto ◽  
Takeshi Kanaseki
Keyword(s):  
Substance P ◽  
Basement Membrane ◽  
Vocal Fold ◽  
Regional Distribution ◽  
Autonomic Innervation ◽  
Taste Bud ◽  
Nerve Fibres ◽  
Motor Endplates ◽  
Calcitonin Gene ◽  
Nerve Bundles

The distribution of substance P (SP) and calcitonin gene-related peptide (CGRP) immunoreactive (ir) fibres in the cat's larynx was investigated utilizing immunohistochemistry. Many SP- and CGRP-ir fibres with varicosities were found within and below the epithelium and along the basement membrane of the mucosa of all different regions except in the membranous portion of the vocal fold. In the subepithelium, some SP- and CGRP-ir nerve bundles and nerve fibres were recognized around the vessels and glands. In the mucosa, the pattern of distribution and the density of SR-ir fibres were similar to those of CGRP-ir fibres. These reactive fibres were denser in the supraglottic region than in the subglottic region. In the taste bud-like structures, only SP-ir fibres appeared, whereas in the motor endplates, CGRP-reaction was found exclusively. The present findings suggest that the regional distribution of SP- and CGRP-immunoreactivity might be related with sensory and autonomic innervation in the larynx.

A New Calcitonin-Receptor-like Sequence in Rat Pulmonary Blood Vessels

1993 ◽  
Vol 85 (4) ◽  
pp. 385-388 ◽  
Author(s):  
F. Njuki ◽  
C. G. Nicholl ◽  
A. Howard ◽  
J. C. W. Mak ◽  
P. J. Barnes ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Vascular Tone ◽  
Islet Amyloid Polypeptide ◽  
Calcitonin Gene Related Peptide ◽  
Binding Activity ◽  
Calcitonin Receptor ◽  
Islet Amyloid ◽  
Related Peptide ◽  
Calcitonin Gene

1. Two rat clones have been isolated which are similar to known calcitonin-receptor sequences. One of these does not have the distribution expected of a calcitonin receptor. It is widely distributed, with extremely high levels of expression in the lung, where it is associated with the blood vessels. 2. This rat sequence may represent the receptor for calcitonin-gene-related peptide or islet amyloid polypeptide. Both have binding activity in the lung and are potent vasodilators. The gene represented by this sequence may therefore play an important role in the maintenance of vascular tone.

Ionomycin stimulates secretion of catecholamines from cat adrenal gland and spleen

1982 ◽  
Vol 242 (3) ◽  
pp. E137-E145 ◽  
Author(s):  
M. H. Carvalho ◽  
J. C. Prat ◽  
A. G. Garcia ◽  
S. M. Kirpekar
Keyword(s):  
Chromaffin Cells ◽  
Adrenal Glands ◽  
Secretory Response ◽  
Adrenergic Nerve ◽  
Nerve Terminals ◽  
Potassium Depolarization ◽  
Calcium Dependent ◽  
Dopamine Beta Hydroxylase ◽  
Adequate Stimulus ◽  
Adrenergic Nerve Terminals

Ionomycin, a polyether antibiotic, stimulated the secretion of catecholamines and dopamine beta-hydroxylase from perfused adrenal glands and [3H]norepinephrine ([3H]NE) from spleens of the cat. Release was calcium dependent, and strontium or barium did not substitute for calcium. Ionomycin failed to release [3H]NE from reserpinized spleens. High magnesium did not interfere in the ionomycin response, but lanthanum and manganese blocked it. Ionomycin response that was pH dependent was not affected by potassium depolarization. The secretory response to ionomycin was enhanced when both glycolysis and oxidative metabolism were inhibited. It is concluded that ionomycin introduces calcium into the chromaffin cells and adrenergic nerve terminals to cause the secretory response and that a rise in intracellular calcium may be an adequate stimulus for secretion.

Abstract 304: The Role of Calcitonin Gene Related Peptide (CGRP) in Cardiovascular Regulation and Blood Vessel Structure

Hypertension ◽  
2013 ◽  
Vol 62 (suppl_1) ◽  
Author(s):  
Tu Mai ◽  
Andre' Diedrich ◽  
Pandu Gangula ◽  
Emily Garland ◽  
David Robertson
Keyword(s):  
Blood Vessel ◽  
Blood Vessels ◽  
Baroreflex Sensitivity ◽  
Cardiovascular Regulation ◽  
Calcitonin Gene Related Peptide ◽  
Activity Level ◽  
Related Peptide ◽  
Calcitonin Gene ◽  
Significant Difference ◽  
Vessel Structure

Calcitonin gene related peptide (CGRP) is known to be a potent microvascular dilator and hypotensive agent. This peptide and its receptors are distributed widely in both central and peripheral nervous systems. CGRP-containing neural fibers are closely associated with blood vessels in the arterial circulation where its release leads to vascular dilation. However, its role in cardiovascular regulation remains controversial. We studied the hemodynamic and autonomic function, as well as blood vessel structure in CGRP knockout (KO) mice. Blood pressure (BP), heart rate (HR) and activity level were assessed at least one week after mice were implanted with telemeters. Ten CGRP KO and ten wild type (WT) mice (4-5 month old) were used in this study. After the recording period, 24-hr urine and blood were collected for assessment of catecholamines and their metabolites. To study the baroreflex sensitivity, phenylephrine and sodium nitroprusside were administered in an acute study with seven mice from each group. We found no significant difference in 24-hr mean arterial pressure (MAP) (105.0 ± 1.8mmHg, vs. 112.1 ± 3.1mmHg, p= 0.05) between the two groups. There was also no difference in HR (KO: 566.4 ± 12.1; WT: 581.9 ± 8.5, p = 0.31). Norepinephrine was greatly elevated in both plasma and 24-hr urine in KO mice (in urine: KO: 956 ± 91 pg/ml, WT: 618 ± 46pg/ml, p = 0.004; in plasma: KO: 2505 ± 596pg/ml, n=6, WT: 1168 ± 98 pg/ml, p = 0.04). Paradoxically, cardiovagal baroreflex sensitivity was higher in the KO mice (KO: 3.2 ± 0.7 ms/mmHg, n=7, WT: 1.4 ± 0.3 ms/mmHg, n=7, p=0.03). Thoracic aortas were collected from both groups to measure vessel stiffness. The diameter at 125 mmHg of the aorta was significantly smaller in CGRP KO mice than in WT (321±30μm, vs. 422±82μm, p<0.01). Therefore, we hypothesize that although CGRP may have a limited contribution to physiological cardiovascular regulation, it may play a significant role in modulating the vagal tone and the structure properties of blood vessels.

Abnormal skin development in pupoid foetus (pf/pf) mutant mice

Development ◽  
1985 ◽  
Vol 87 (1) ◽  
pp. 47-64
Author(s):  
Chris Fisher ◽  
Edward J. Kollar
Keyword(s):  
Blood Vessels ◽  
Schwann Cells ◽  
Cell Population ◽  
Epidermal Cell ◽  
Basal Lamina ◽  
Mutant Mice ◽  
Nerve Fibres ◽  
Skin Development ◽  
Cell Layers

At 13 days of development the epidermis of mice homozygous for the pupoid foetus (pf/pf) mutation varies in thickness between one and ten cell layers. By 16 days of development cells from the dermis have invaded the epidermis and may be found throughout the epidermis and on its surface. Among these cells are nerve fibres and Schwann cells as well as other unidentified cells. Antibodies directed against fibronectin bind to these abnormal groups of cells in the mutant epidermis and on its surface. A basal lamina, as determined by ultrastructure and by the immuno-fluorescent localization of laminin, was always found at the interface of the mutant epidermis and the invading cell population. By 19 days of development the mutant epidermis is thickened and is permeated by a network of cells including nerve fibres, Schwann cells, blood vessels, and collagen and fibronectin-secreting cells. A basal lamina always separates these groups of invading cells from the epidermal cell population.

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