PIWIs maintain testis apoptosis to remove abnormal germ cells in Eriocheir sinensis

Reproduction ◽  
2021 ◽  
Author(s):  
Bang-Hong Wei ◽  
Jia-Hao Ni ◽  
Tong Yang ◽  
Shuang-Li Hao ◽  
Wan-Xi Yang
Keyword(s):  
B Cell ◽  
Germ Cells ◽  
Cell Lymphoma ◽  
B Cell Lymphoma ◽  
Eriocheir Sinensis ◽  
Germline Development ◽  
P53 Pathway ◽  
Apoptosis Pathway ◽  
Encoded Proteins

PIWI proteins play important roles in germline development in the mammals. However, the functions of PIWIs in crustaceans remain unknown. In the present study, we identified three Piwis from the testis of Eriocheir sinensis (E. sinensis). Three Piwi genes encoded proteins with typical features of PIWI subfamilies and were highly expressed in the testis. Three PIWIs could be detected in the cytoplasm of spermatocytes and spermatids, while in spermatozoa, we could only detect PIWI1 and PIWI3 in the nucleus. The knockdown of PIWIs by dsRNA significantly affected the formation of the nuclei in spermatozoa, which resulted in deviant and irregular nuclei. PIWI defects significantly inhibited the apoptosis of abnormal germ cells through the caspase-dependent apoptosis pathway and p53 pathway. Knockdown of PIWIs inhibited the expression of caspase3, 7, 8, and p53 without affecting Bcl2 (B-cell lymphoma gene 2), Bax (B-cell lymphoma-2-associated X) and BaxI (B-cell lymphoma-2-associated X inhibitor), which further significantly increased abnormal spermatozoa in the knockdown-group crabs. These results show a new role of PIWI proteins in crustaceans that is different from that in mammals. In summary, PIWIs play roles in the formation of the germline nucleus and can maintain apoptosis in abnormal germ cells to remove abnormal germ cells in E. sinensis.

Acute kidney injury secondary to renal large B-cell lymphoma: role of early renal biopsy

2010 ◽  
Vol 43 (1) ◽  
pp. 237-240 ◽  
Author(s):  
Suhail Al-Salam ◽  
Ahmad Shaaban ◽  
Maha Alketbi ◽  
Naveed U. Haq ◽  
Samra Abouchacra
Keyword(s):  
Acute Kidney Injury ◽  
B Cell ◽  
Renal Biopsy ◽  
Cell Lymphoma ◽  
Kidney Injury ◽  
B Cell Lymphoma ◽  
Large B Cell Lymphoma ◽  
Large B Cell

Role of WT1, B-cell lymphoma 2, Ki-67 (Mib1), and Her2/Neu as diagnostic and prognostic immunomarkers in ovarian serous and endometroid carcinoma

2021 ◽  
Vol 17 (1) ◽  
pp. 166
Author(s):  
SantoshKumar Mondal ◽  
Bijan Basak ◽  
Saptarshi Bhattacharya ◽  
UtpalKumar Panda
Keyword(s):  
B Cell ◽  
Cell Lymphoma ◽  
B Cell Lymphoma ◽  
Ki 67

scholarly journals New molecular and therapeutic insights into canine diffuse large B-cell lymphoma elucidates the role of the dog as a model for human disease

Haematologica ◽  
2018 ◽  
Vol 104 (6) ◽  
pp. e256-e259 ◽  
Author(s):  
Luca Aresu ◽  
Serena Ferraresso ◽  
Laura Marconato ◽  
Luciano Cascione ◽  
Sara Napoli ◽  
...  
Keyword(s):  
B Cell ◽  
Human Disease ◽  
Cell Lymphoma ◽  
B Cell Lymphoma ◽  
Large B Cell Lymphoma ◽  
Large B Cell

The Role of Transplantation in Diffuse Large B-Cell Lymphoma: The Impact of Rituximab Plus Chemotherapy in First-line and Relapsed Settings

2010 ◽  
Vol 6 (1) ◽  
pp. 47-57 ◽  
Author(s):  
Celso Arrais Rodrigues ◽  
Poliana Alves Patah ◽  
Yana A. S. Novis ◽  
Chitra Hosing ◽  
Marcos de Lima
Keyword(s):  
B Cell ◽  
Cell Lymphoma ◽  
B Cell Lymphoma ◽  
First Line ◽  
Large B Cell Lymphoma ◽  
The Impact ◽  
Large B Cell

The Role of Radiation Therapy in Refractory or Relapsing Diffuse Large B-Cell Lymphoma

2018 ◽  
Vol 102 (3) ◽  
pp. e251
Author(s):  
E. Grignano ◽  
J. Laurent ◽  
B. Deau-Fisher ◽  
B. Burroni ◽  
D. Bouscary ◽  
...  
Keyword(s):  
Radiation Therapy ◽  
B Cell ◽  
Cell Lymphoma ◽  
B Cell Lymphoma ◽  
Large B Cell Lymphoma ◽  
Large B Cell

Role of radiotherapy in patients with limited diffuse large B-cell lymphoma of Waldeyer's ring in remission after R-CHOP immunochemotherapy

2018 ◽  
Vol 74 ◽  
pp. 80-85
Author(s):  
Chunyan Li ◽  
Xuejun Ma ◽  
Ziqiang Pan ◽  
Fangfang Lv ◽  
Zuguang Xia ◽  
...  
Keyword(s):  
B Cell ◽  
Cell Lymphoma ◽  
B Cell Lymphoma ◽  
Waldeyer’S Ring ◽  
Large B Cell Lymphoma ◽  
Large B Cell

scholarly journals The Role of Caspase-3, Apoptosis-Inducing Factor, and B-cell Lymphoma-2 Expressions in Term Premature Rupture of Membrane

2018 ◽  
Vol 40 (12) ◽  
pp. 733-739
Author(s):  
Ketut Negara ◽  
Ketut Suwiyoga ◽  
Tjokorda Pemayun ◽  
Anak Agung Sudewi ◽  
Nyoman Astawa ◽  
...  
Keyword(s):  
B Cell ◽  
Caspase 3 ◽  
Cell Lymphoma ◽  
B Cell Lymphoma ◽  
Term Pregnancy ◽  
Premature Rupture ◽  
Term Delivery ◽  
Chi Squared ◽  
Apoptosis Inducing Factor

Objective To determine the role of caspase-3, apoptosis-inducing factor (AIF), and B-cell lymphoma-2 (Bcl-2) expressions in term premature rupture of membrane (PROM). Methods An analytic observational study with case-control design was conducted, involving 52 subjects (37–42 weeks of gestation) who were divided into 2 groups: 26 cases of term delivery with PROM, and 26 controls of term delivery without PROM. The expressions of caspase-3, AIF, and Bcl-2 in the amniotic membrane were determined by immunohistochemistry. Data were analyzed using the chi-squared test. The risk of PROM was expressed by odds ratio (OR). Results There were no significant differences in age, parity and body mass index between the two groups (p > 0.05). High caspase-3 and AIF expressions increased the risk of PROM 17.64 times (OR = 17.64; 95% CI = 4.44–70.07; p = 0.001) and 9.45 times (OR = 9.45; 95% CI= 2.62–34.07; p = 0.001), respectively, while low Bcl-2 expression increased 10.39 times (OR = 10.39; 95% CI = 2.73–39.56; p = 0.001)the risk of PROM . Conclusion High caspase-3 and AIF expressions and low Bcl-2 expression were risk factors for term PROM. Caspase-dependent and independent pathways of apoptosis were involved in the mechanism of PROM in term pregnancy.

scholarly journals Targeting mitochondrial respiration and the BCL2 family in MYC-associated B-cell lymphoma

2020 ◽  
Author(s):  
Giulio Donati ◽  
Micol Ravà ◽  
Marco Filipuzzi ◽  
Paola Nicoli ◽  
Laura Cassina ◽  
...  
Keyword(s):  
Oxidative Phosphorylation ◽  
B Cell ◽  
Complex I ◽  
Cell Lymphoma ◽  
B Cell Lymphoma ◽  
High Grade ◽  
Apoptosis Pathway ◽  
Bcl2 Family ◽  
Molecular Features ◽  
Intrinsic Apoptosis Pathway

AbstractMultiple molecular features, such as activation of specific oncogenes (e. g. MYC, BCL2) or a variety of gene expression signatures, have been associated with disease course in diffuse large B-cell lymphoma (DLBCL). Understanding the relationships between these features and their possible exploitation toward disease classification and therapy remains a major priority in the field. Here, we report that MYC activity in DLBCL is closely correlated with – and most likely a driver of – gene signatures related to Oxidative Phosphorylation (OxPhos). On this basis, we hypothesized that enzymes involved in Oxidative Phosphorylation, and in particular electron-transport chain (ETC) complexes, might constitute tractable therapeutic targets in MYC-associated lymphoma. Indeed, our data show that MYC sensitizes B-cells to IACS-010759, a selective inhibitor of ETC complex I. Mechanistically, IACS-010759 activates an ATF4-driven Integrated Stress Response (ISR), engaging the intrinsic apoptosis pathway through the transcription factor CHOP. In line with these findings, IACS-010759 shows synergy with the BCL2 inhibitor venetoclax against double-hit lymphoma (DHL), a high-grade form of DLBCL with concurrent activation of MYC and BCL2. Similarly, in BCL2-negative lymphoma cell lines, inhibition of the BCL2-related protein Mcl-1 potentiates killing by IACS-010759. Altogether, ETC complex I inhibition engages the ISR to lower the apoptotic threshold in MYC-driven lymphomas and, in combination with select BCL2-family inhibitors, provides a novel therapeutic principle against this aggressive DLBCL subset.Statement of significanceThis work points to OxPhos as a key MYC-activated process and a tractable therapeutic target toward personalized treatment of high-grade DLBCL, providing strong context-dependent cooperation with BH3-mimetic compounds.

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