scholarly journals Low amounts of mitochondrial reactive oxygen species define human sperm quality

Reproduction ◽  
2014 ◽  
Vol 147 (6) ◽  
pp. 817-824 ◽  
Author(s):  
Mónica Marques ◽  
Ana Paula Sousa ◽  
Artur Paiva ◽  
Teresa Almeida-Santos ◽  
João Ramalho-Santos
Keyword(s):  
Reactive Oxygen Species ◽  
Assisted Reproduction ◽  
Density Gradient Centrifugation ◽  
Sperm Quality ◽  
Gradient Centrifugation ◽  
Reactive Oxygen ◽  
Human Sperm ◽  
Semen Parameters ◽  
Oxygen Species ◽  
Male Gametes

We have applied the mitochondria-specific superoxide fluorescent probe MitoSOX Red (MitoSOX) to detect mitochondria-specific reactive oxygen species (mROS) production in human sperm samples using flow cytometry. We show that human ejaculates are heterogeneous in terms of mROS production, with three subpopulations clearly detectable, comprising sperm that produce increasing amounts of mROS (MitoSOX−, MitoSOX+, and MitoSOX++). The sperm subpopulation producing the lowest amount of mROS represented the most functional subset of male gametes within the ejaculate, as it was correlated with the highest amount of live and non-apoptotic sperm and increased both in samples with better semen parameters and in samples processed by both density-gradient centrifugation and swim-up, both known to select for higher quality sperm. Importantly, the MitoSOX− subpopulation was clearly more prevalent in samples that gave rise to pregnancies following assisted reproduction. Our work, therefore, not only describe discreet human sperm heterogeneity at the mROS level but also suggests that mROS may represent a strategy to both evaluate sperm samples and isolate the most functional gametes for assisted reproduction.Free Portuguese abstractA Portuguese translation of this abstract is freely available athttp://www.reproduction-online.org/content/147/6/817/suppl/DC1

scholarly journals Effect of density gradient centrifugation on reactive oxygen species in human semen

2017 ◽  
Vol 63 (3) ◽  
pp. 192-198 ◽  
Author(s):  
Teppei Takeshima ◽  
Yasushi Yumura ◽  
Shinnosuke Kuroda ◽  
Takashi Kawahara ◽  
Hiroji Uemura ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Density Gradient ◽  
Density Gradient Centrifugation ◽  
Gradient Centrifugation ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Human Semen

scholarly journals Defining the Mechanisms by Which the Reactive Oxygen Species By-Product, 4-Hydroxynonenal, Affects Human Sperm Cell Function1

2015 ◽  
Vol 92 (4) ◽  
Author(s):  
Mark A. Baker ◽  
Anita Weinberg ◽  
Louise Hetherington ◽  
Ana-Izabel Villaverde ◽  
Tony Velkov ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Sperm Cell ◽  
Reactive Oxygen ◽  
Human Sperm ◽  
Oxygen Species

Failure of Implantation in IVF due to oxidative stress

2021 ◽  
Vol 20 (2) ◽  
pp. 45-52
Author(s):  
Sofoklis Stavros ◽  
Antonios Koutras ◽  
Thomas Ntounis ◽  
Konstantinos Koukoubanis ◽  
Theodoros Papalios ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Reproductive Tract ◽  
Sperm Quality ◽  
Reactive Oxygen ◽  
Female Reproductive Tract ◽  
Oxygen Species ◽  
Stress Markers ◽  
Multiple Levels ◽  
Antioxidant Mechanisms

Oxidative stress may play a role in implantation failure on multiple levels. Oxidative stress is found widely in several biological systems, as well as it acts on various molecular levels with different mechanisms. It has been shown that it is rather the disequilibrium between reactive oxygen species causing oxidative stress and antioxidant mechanisms counteracting their effects, than reactive oxygen species levels themselves. Reactive oxygen species play a role in implantation and fertilisation by acting on different levels of embryo-formation and endometrial changes. Additionally, it is widely abundant in the female reproductive tract including ovaries, oocytes, tubal as well as follicular fluid. Moreover, it has been shown that male fertility is affected by reactive oxygen species by determining sperm quality. Last but not least, oxidative stress may affect IVF indirectly through its actions on peritoneal fluid. As long as research studies on elucidating the development of oxidative stress markers on patients undergoing IVF continue, ever more new possibilities emerge on predicting the pregnancy outcome.

scholarly journals HUBUNGAN JUMLAH LEUKOSIT DENGAN MOTILITAS SPERMATOZOA PADA ANALISIS SEMEN PRIA DI SAMMARIE FAMILY HEALTHCARE 2019

2020 ◽  
Vol 4 (2) ◽  
pp. 335
Author(s):  
Wanodia Ayutama ◽  
Tuty Rizkianti ◽  
Cut Fauziah
Keyword(s):  
Reactive Oxygen Species ◽  
Reproductive Tract ◽  
Sperm Quality ◽  
Semen Analysis ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Cross Sectional ◽  
Spermatozoa Motility ◽  
Fertile Female ◽  
Leukocyte Counts

Infertility is one of the most common male reproductive health problems. Male infertility is the inability of a male to result pregnancy in a fertile female in one year of non-contracepting sexual intercourse. Male with poor sperm quality are more susceptible to infertility. One of the cause of infertility in men is disruption of spermatozoa motility. Imperfect motility will reduce the quality of spermatozoa and the probability of conception. One cause of decreased motility is inflammation of the male reproductive tract. Inflammation that occurs will increase the recruitment of leukocytes in the reproductive tract and increase the production of reactive oxygen species (ROS) so it can interfere the process of sperm formation and maturation. The purpose of this study was to determine the relationship between leukocyte counts and spermatozoa motility. The number of leukocytes and the percentage of spermatozoa motility were obtained from semen analysis as secondary data. The research design used was cross-sectional. The number of samples in this study were 66 respondents who met the inclusion criteria.The results of the study showed a significant inverse relationship (negative correlation) (p = 0.007, r = -0.328) between the number of leukocytes and spermatozoa motility, which means  if the number of semen leukocytes increases, the percentage of spermatozoa motility will decrease. Keywords: inflammation; leukocyte counts; spermatozoa motility ABSTRAKInfertilitas merupakan salah satu masalah kesehatan reproduksi pria yang sering dijumpai. Infertilitas pada pria adalah ketidakmampuan seorang pria untuk menyebabkan kehamilan pada seorang wanita fertil setelah satu tahun hubungan seksual tanpa alat kontrasepsi. Pria dengan kualitas sperma yang kurang baik lebih rentan mengalami infertilitas. Salah satu penyebab infertilitas pada pria adalah gangguan pada motilitas spermatozoa. Motilitas yang kurang sempurna akan menyebabkan penurunan kualitas spermatozoa dan penurunan probabilitas terjadinya pembuahan. Salah satu penyebab penurunan motilitas adalah inflamasi pada saluran reproduksi pria. Inflamasi yang terjadi akan meningkatkan rekruitmen leukosit pada saluran  reproduksi pria dan meningkatkan produksi reactive oxygen species (ROS) yang bersifat toksik bagi spermatozoa sehingga dapat mengganggu proses pembentukan dan pematangan spermatozoa. Tujuan penelitian ini adalah untuk mengetahui hubungan antara jumlah leukosit dengan motilitas spermatozoa. Jumlah leukosit dan persentase motilitas spermatozoa didapatkan dari data sekunder, yaitu data hasil analisis semen. Desain penelitian yang digunakan adalah potong lintang (cross-sectional). Jumlah sampel dalam penelitian ini sebanyak 81 pasien yang memenuhi kriteria inklusi. Hasil dari penelitian menunjukkan terdapat hubungan terbalik (korelasi negatif) yang bermakna dengan kekuatan korelasi sangat lemah (p = 0.007, r = -0.328) antara jumlah leukosit dengan motilitas spermatozoa, yang berarti jika jumlah leukosit semen semakin meningkat, maka persentase motilitas spermatozoa akan semakin rendah.

scholarly journals Influence of PM2.5 on spermatogenesis dysfunction via the reactive-oxygen-species-mediated Mitogen-activated-protein-kinase signaling pathway

2019 ◽  
Vol 6 (4) ◽  
pp. 77-79
Author(s):  
Ruangrong Cheepsattayakorn
Keyword(s):  
Reactive Oxygen Species ◽  
Signaling Pathway ◽  
Reactive Oxygen ◽  
Human Sperm ◽  
Mapk Signaling ◽  
Autoimmune Response ◽  
Sperm Chromatin ◽  
Seminiferous Tubules ◽  
Oxygen Species ◽  
Mitogen Activated Protein

Approximately 15 % of the world‘s couples confront childless, and about 50 % of them are due to male reproductive disorders. Several previous studies demonstrated that PM2.5 particles has been consistently associated with critical human sperm reduction and impairment of human sperm chromatin and DNA from traffic exhaust pollution. Blood-testis barrier (BTB), a critically physical barrier between the seminiferous tubules and the blood vessels prevents sperm antigens from entering the blood circulation and facilitating and initiating an autoimmune response that contributing to spermatogenesis interference. Reactive oxygen species (ROS) are involved in the redox-sensitive signal transduction factors activation, such as Jun NH2-terminal kinase (JNK), p 38, extracellular signal-regulated kinase (ERK), and mitogen-activated protein kinases (MAPK) that critically influence BTB disruption. After PM2.5 exposure, there are decreased superoxide dismutase (SOD) expression, increased malondialdehyde (MDA) expression, increased nuclear factor erythroid 2-related factor 2 (Nrf-2) expression, increased expression of the four junctional proteins (β-catenin, Cx43, occludin, zonula occludens-1 (ZO-1)), thus improve sperm quality and quantity. PM2.5 particles markedly induce increasing phosphorylation of MAPKs via the ROS-mediated MAPK signaling pathway that causes BTB disruption, but this effect is lesser in the vitamins C and E intervention as well as increasing cleaved caspase-3 expression and the Bcl-2/Bax ratio. In conclusion, combined therapeutic administration of vitamins C and E can maintain the BTB integrity, reduce oxidative stress and cell apoptosis, and prevent toxic effects.

A Study of Role of Reactive Oxygen Species in Idiopathic Male Infertility & Its Management by Antioxidant Therapy in Uttar Pradesh (U.P.)

2021 ◽  
Vol 8 (32) ◽  
pp. 3023-3027
Author(s):  
Namrata Shrivastava ◽  
Vaibhav Shrivastava ◽  
Manish Pandey
Keyword(s):  
Reactive Oxygen Species ◽  
Male Infertility ◽  
Reactive Oxygen ◽  
Antioxidant Therapy ◽  
Semen Parameters ◽  
Control Group ◽  
P Value ◽  
Ros Generation ◽  
Variable Degree ◽  
Oxygen Species

BACKGROUND Infertility is defined as the inability to conceive after at 1 year of regular unprotected intercourse. Male contributes to almost half of infertility cases and in almost 30 % of cases, no definite aetiology is identified, and hence, male infertility is labelled idiopathic in these cases. Oxidative energy production mechanisms are almost always accompanied by reactive oxygen species (ROS), generation whose too much concentrations can lead to extensive protein damage and cytoskeletal modifications and inhibit cellular mechanisms. A number of laboratory techniques have been developed to evaluate oxidative stress by measuring ROS level in the semen. In recent times antioxidant supplements have been proposed as useful agents to increase the scavenging capacity of seminal plasma, controversy still surrounds their actual clinical utility. METHODS 34 male patients were included in this study. Reactive oxygen species detection was done by Flowcytometry using dichloroflurosecindiacetate (DCFH-DA). RESULTS The ROS in the patient group was found to be significantly higher 29.821 (5.6300 than the control group 22.162 (1.6331 having p value < 0.001). The ROS (29.821 ± 5.6300) was found to be significantly reduced after 3 months of antioxidant therapy which got reduced to 19.893 ± 4.2299 respectively. CONCLUSIONS Our study demonstrates that infertile men have significantly higher level of ROS (as measured by flowcytometry) & lower sperm count (oligospermia), decreased progressive & total motility and increased immotile sperms as compared to healthy fertile men. This study further proves that antioxidant therapy based on a combination of carnitine, zinc, coq10, lycopene and vitamin C & E for 3 months is associated with a decrease of ROS as measured by flowcytometry & a variable degree of improvement in above mentioned semen parameters. KEYWORDS Reactive Oxygen Species, Male Infertility

scholarly journals Selection of Functional Human Sperm with Higher DNA Integrity and Fewer Reactive Oxygen Species

2014 ◽  
Vol 3 (10) ◽  
pp. 1671-1679 ◽  
Author(s):  
Waseem Asghar ◽  
Vanessa Velasco ◽  
James L. Kingsley ◽  
Muhammad S. Shoukat ◽  
Hadi Shafiee ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Human Sperm ◽  
Dna Integrity ◽  
Oxygen Species ◽  
Selection Of
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