scholarly journals IGF2, IGF binding protein 1, and matrix metalloproteinases 2 and 9 in implantation-stage endometrium following immunoneutralization of vascular endothelial growth factor in the rhesus monkey

Reproduction ◽  
2011 ◽  
Vol 141 (4) ◽  
pp. 501-509 ◽  
Author(s):  
D Ghosh ◽  
A R Najwa ◽  
M A Khan ◽  
J Sengupta
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Trophoblast Cell ◽  
Extravillous Trophoblast ◽  
Vascular Endothelial ◽  
Trophoblast Cells ◽  
Igf Binding ◽  
Igf Binding Protein ◽  
Endothelial Growth Factor ◽  
Control Samples

Blastocyst implantation in the rhesus monkey is inhibited by administration of antibody against vascular endothelial growth factor (VEGF) A during peri-implantation period with no change in the circulatory concentrations of estradiol, progesterone, and VEGF. In this study, we have investigated the effect of administration of a MAB to VEGFA on days 5 and 10 after ovulation upon the mRNA expression, immunopositive protein expression, and immunohistological localization of IGF2, IGF binding protein 1 (IGFBP1) and matrix metalloproteinases (MMPs) 2 and 9 in the implantation-stage endometrium collected on day 13 after ovulation from fecund cycles of rhesus monkeys. The comparison between isotype-matched IgG (control; n=8)- and VEGF antibody (VEGF Mab; n=8)-treated animals revealed higher (P<0.05) IGF2 in lacunar and villous syncytiotrophoblasts, trophoblast cell columns, migrating extravillous trophoblast cells, and endovascular trophoblast cells in control animals, but with no change in the various cell types of maternal endometrium between the two groups. No change in IGFBP1 expression in the endometrium was observed between the two groups. MMPs 2 and 9 were detected in syncytiotrophoblast in lacunae and villi, trophoblast cell columns, and extravillous trophoblast cells in control samples. MMP9 transcript expression in maternal endometrium and its immunopositivity in endometrial stroma and trophoblast cells were lower (P<0.05) with no change in MMP2 level in VEGF Mab-exposed samples compared with those in control samples. A functional network involving VEGF, IGF2, and MMP9 in early placental trophoblast cells and maternal endometrium appears to be important for normal placentation.

Effects of shRNA Targeting Maspin on the Invasion of Extravillous Trophoblast Cell

2017 ◽  
Vol 34 (10) ◽  
pp. 0966-0973 ◽  
Author(s):  
Xinwei Shi ◽  
Qing Liu ◽  
Hao Liu ◽  
Dongrui Deng ◽  
Fuyuan Qiao ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Polymerase Chain Reaction ◽  
Growth Factor ◽  
Trophoblast Cell ◽  
Extravillous Trophoblast ◽  
Vascular Endothelial ◽  
Chain Reaction ◽  
Maspin Expression ◽  
Polymerase Chain ◽  
Endothelial Growth Factor

Objective Hypomethylation of the maspin gene results in increased expression of maspin in preeclamptic placentas. However, maspin gene function and the molecular aspects in placentation remain largely unclear. The study was designed to investigate the effects of maspin on the invasion of extravillous trophoblast cell line (TEV-1) and the molecular mechanism. Study Design We cloned short hairpin RNA (shRNA) targeting maspin gene into plasmid pGenesil-1.1 eukaryotic expression vector and then transfected it using adenovirus. The methylation rates in the maspin gene were detected by bisulfite sequencing polymerase chain reaction; the invasive ability of trophoblast cells was examined by Transwell chamber assay; the mRNA and protein expression of maspin and some invasive related gene was detected by reverse transcription-polymerase chain reaction and Western blot analysis. Results After the maspin expression was successfully knocked down, the methylation rates in the maspin gene were significantly increased, and the number of cells invading through Matrigel (Corning Life Sciences and BD Biosciences) was obviously increased. The mRNA levels of vascular endothelial growth factor-A (VEGF-A), vascular endothelial growth factor-C (VEGF-C), and matrix metalloproteinase-2 (MMP2) were increased significantly. Conclusion Using shRNA technology, this study further verified that maspin gene methylation could decrease maspin expression and inhibit the invasion of TEV-1 cells through VEGF-A, VEGF-C, and MMP2.

Expression and Regulation of Vascular Endothelial Growth Factor in a First Trimester Trophoblast Cell Line

Placenta ◽  
2000 ◽  
Vol 21 (4) ◽  
pp. 320-324 ◽  
Author(s):  
I-B. Chung ◽  
F.D. Yelian ◽  
F.M. Zaher ◽  
B. Gonik ◽  
M.I. Evans ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Cell Line ◽  
First Trimester ◽  
Trophoblast Cell ◽  
Vascular Endothelial ◽  
Endothelial Growth Factor ◽  
Trophoblast Cell Line
Sign in / Sign up

Export Citation Format

Share Document