scholarly journals Regulators of mitotic proliferation in mouse primordial germ cells

Reproduction ◽  
2009 ◽  
Vol 138 (1) ◽  
pp. 185
Author(s):  
Massimo De Felici ◽  
Eleonora Sorrentino ◽  
Francesca Klinger ◽  
Cecilia De Felici ◽  
Donatella Farini
Keyword(s):  
Germ Cells ◽  
Primordial Germ Cells ◽  
Mitotic Proliferation

scholarly journals XY oocytes of sex-reversed females with a Sry mutation deviate from the normal developmental process beyond the mitotic stage†

2018 ◽  
Vol 100 (3) ◽  
pp. 697-710 ◽  
Author(s):  
Akihiko Sakashita ◽  
Takuya Wakai ◽  
Yukiko Kawabata ◽  
Chiaki Nishimura ◽  
Yusuke Sotomaru ◽  
...  
Keyword(s):  
Germ Cells ◽  
Developmental Process ◽  
Sex Differentiation ◽  
Primordial Germ Cells ◽  
Primordial Follicle ◽  
Genetic Ablation ◽  
Female Mice ◽  
Meiotic Progression ◽  
Molecular Etiology ◽  
Mitotic Proliferation

Abstract The fertility of sex-reversed XY female mice is severely impaired by a massive loss of oocytes and failure of meiotic progression. This phenomenon remains an outstanding mystery. We sought to determine the molecular etiology of XY oocyte dysfunction by generating sex-reversed females that bear genetic ablation of Sry, a vital sex determination gene, on an inbred C57BL/6 background. These mutant mice, termed XYsry− mutants, showed severe attrition of germ cells during fetal development, resulting in the depletion of ovarian germ cells prior to sexual maturation. Comprehensive transcriptome analyses of primordial germ cells (PGCs) and postnatal oocytes demonstrated that XYsry− females had deviated significantly from normal developmental processes during the stages of mitotic proliferation. The impaired proliferation of XYsry− PGCs was associated with aberrant β-catenin signaling and the excessive expression of transposable elements. Upon entry to the meiotic stage, XYsry− oocytes demonstrated extensive defects, including the impairment of crossover formation, the failure of primordial follicle maintenance, and no capacity for embryo development. Together, these results suggest potential molecular causes for germ cell disruption in sex-reversed female mice, thereby providing insights into disorders of sex differentiation in humans, such as “Swyer syndrome,” in which patients with an XY karyotype present as typical females and are infertile.

scholarly journals MRG-1, a mortality factor-related chromodomain protein, is required maternally for primordial germ cells to initiate mitotic proliferation in C. elegans

2002 ◽  
Vol 114 (1-2) ◽  
pp. 61-69 ◽  
Author(s):  
Masaki Fujita ◽  
Teruaki Takasaki ◽  
Noboru Nakajima ◽  
Taizo Kawano ◽  
Yoshiro Shimura ◽  
...  
Keyword(s):  
Germ Cells ◽  
Primordial Germ Cells ◽  
Mortality Factor ◽  
C Elegans ◽  
Mitotic Proliferation

A Quantitative Study of Primordial Germ Cells in the Male Rat

Development ◽  
1963 ◽  
Vol 11 (4) ◽  
pp. 715-740
Author(s):  
Heather M. Beaumont ◽  
Anita M. Mandl
Keyword(s):  
Quantitative Study ◽  
Germ Cells ◽  
Total Population ◽  
Developmental Stages ◽  
Sex Differentiation ◽  
Primordial Germ Cells ◽  
Male Rat ◽  
Female Rat ◽  
Mitotic Proliferation ◽  
Prophase Of Meiosis

In mammals, as in other vertebrates, primordial germ cells arise extra-gonadally and migrate to the genital ridges (see Franchi, Mandl & Zuckerman, 1962). In the rat, the gonads undergo sex differentiation on the 14th day of gestation, i.e. some 2 days after the arrival of the primordial germ cells. In the female rat, the number of oögonia increases sharply, due to active mitotic proliferation, between the 14th and 18th day of gestation. Thereafter, the majority of germ cells enter the prophase of meiosis, and thus, by definition, become oöcytes. A large number of oöcytes undergo spontaneous degeneration, with the result that the total population decreases from a peak of about 75,000 (at 18·5 days) to about a third that number 2 days after birth. The decrease in the population is due to three distinct ‘waves’ of degeneration occurring at specific developmental stages (Beaumont & Mandl, 1962).

Hexachlorobenzene toxicity in the rat ovary: II. Ultrastructure induced by medium (10 mg/kg) dose exposure

1992 ◽  
Vol 50 (1) ◽  
pp. 668-669
Author(s):  
Amreek Singh ◽  
Warren G. Foster ◽  
Anna Dykeman ◽  
David C. Villeneuve
Keyword(s):  
Germ Cells ◽  
Primordial Germ Cells ◽  
Surface Epithelium ◽  
Morphological Parameters ◽  
Comprehensive Investigation ◽  
Ovary Surface Epithelium ◽  
Rat Ovary ◽  
High Doses

Hexachlorobenzene (HCB) is a known toxicant that is found in the environment as a by-product during manufacture of certain pesticides. This chlorinated chemical has been isolated from many tissues including ovary. When administered in high doses, HCB causes degeneration of primordial germ cells and ovary surface epithelium in sub-human primates. A purpose of this experiment was to determine a no-effect dose of the chemical on the rat ovary. The study is part of a comprehensive investigation on the effects of the compound on the biochemical, hematological, and morphological parameters in the monkey and rat.

Transfusion of Chick Primordial Germ Cells into Quail Embryos and their Settlement in Gonads

1998 ◽  
Vol 69 (10) ◽  
pp. 911-915 ◽  
Author(s):  
Tamao ONO ◽  
Ryohei YOKOI ◽  
Seishi MAEDA ◽  
Takao NISHIDA ◽  
Hirohiko AOYAMA
Keyword(s):  
Germ Cells ◽  
Primordial Germ Cells
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