scholarly journals Expression and regulation of fucosyltransferase 4 in human endometrium

Reproduction ◽  
2008 ◽  
Vol 136 (1) ◽  
pp. 117-123 ◽  
Author(s):  
Anna P Ponnampalam ◽  
Peter A W Rogers
Keyword(s):  
Menstrual Cycle ◽  
Protein Expression ◽  
Real Time Pcr ◽  
Hormonal Regulation ◽  
Inductive Effect ◽  
Human Endometrium ◽  
Luminal Epithelium ◽  
Selectin Ligands ◽  
Initial Adhesion ◽  
Endometrial Epithelium

It has been suggested that selectin ligands expressed by the endometrial epithelium are essential for the initial adhesion of the blastocyst to the luminal epithelium of human endometrium. One of the enzymes responsible for the production of selectin ligands is fucosyltransferase 4 (FUT4), a member of α1,3 fucosyltransferases. The aims of the present study were to characterize FUT4 mRNA and protein in human endometrium during the menstrual cycle and to investigate the hormonal regulation of FUT4 whose mRNA expression was quantified by real-time PCR in fresh endometrial tissue from cycling women and protein expression was analyzed by immunohistochemistry and Western blotting. Hormonal regulation of FUT4 transcription was investigated using an endometrial explant system. FUT4 mRNA was significantly upregulated in fresh tissues during early and mid-secretory phases when compared with other phases of the menstrual cycle. FUT4 protein was localized to glandular and luminal epithelium and the expression levels followed the same pattern as for FUT4 mRNA. Our data also show that, in proliferative explants, progesterone significantly increased FUT4 transcription and translation after 24 h in culture. The inductive effect of progesterone on FUT4 transcription was lost after 48 h of treatment. Estrogen did not have any significant effects. These data suggest that the upregulation of selectin ligands in the human endometrium at the time of implantation may be mediated, at least in part, by the regulation of FUT4 expression.

scholarly journals Hypoxia-Inducible Factor-1α Expression in Human Endometrium and Its Regulation by Prostaglandin E-Series Prostanoid Receptor 2 (EP2)

Endocrinology ◽  
2006 ◽  
Vol 147 (2) ◽  
pp. 744-753 ◽  
Author(s):  
Hilary O. D. Critchley ◽  
Julia Osei ◽  
Teresa A. Henderson ◽  
Lyndsey Boswell ◽  
Kurt J. Sales ◽  
...  
Keyword(s):  
Menstrual Cycle ◽  
Protein Expression ◽  
Transcriptional Activation ◽  
Hypoxia Inducible Factor ◽  
Growth Factor Receptor ◽  
Human Endometrium ◽  
Prostaglandin E ◽  
Menstrual Phase ◽  
Prostanoid Receptor ◽  
Glandular Cells

The menstrual cycle is a complex interaction of sex steroids, prostanoids, and cytokines that lead to coordinated tissue degradation, regeneration and repair. The transcription factor hypoxia-inducible factor (HIF-1) plays critical roles in cellular responses to hypoxia, the generation of an inflammatory response and vasculogenesis through transcriptional activation of angiogenic genes. We hypothesize that HIF-1 is expressed in human endometrium and that locally synthesized prostaglandins (PGE2 and PGF2α) regulate HIF-1 activity. Here we demonstrate that PGE2 up-regulates HIF-1α mRNA and protein via the E-series prostanoid receptor 2 (EP2), and this up-regulation is dependent on epidermal growth factor receptor kinase activity. We show the tight temporal-spatial confinement of HIF-1α protein expression in endometrium across the cycle. HIF-1α is expressed exclusively during the secretory and menstrual phases. Protein expression is maximal at progesterone withdrawal during the late secretory and menstrual phase. HIF-1α protein colocalizes with prostaglandin EP2 receptor in glandular cells. In contrast, HIF-1β/aryl receptor nuclear translocator 1 expression occurs throughout the cycle but is maximal in glandular cells during the proliferative phase. This provides evidence for a role for HIF-1 in the menstrual cycle and demonstrates that HIF-1 activation in human endometrium may occur via a PGE2-regulated pathway and provides a coordinated pathway from progesterone withdrawal through to angiogenic gene expression via HIF-1.

scholarly journals How Mechanical Forces Change the Human Endometrium during the Menstrual Cycle in Preparation for Embryo Implantation

Cells ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 2008
Author(s):  
Anna K. Sternberg ◽  
Volker U. Buck ◽  
Irmgard Classen-Linke ◽  
Rudolf E. Leube
Keyword(s):  
Menstrual Cycle ◽  
Hormonal Regulation ◽  
Embryo Implantation ◽  
Human Endometrium ◽  
Force Balance ◽  
Cell Behavior ◽  
Mechanical Forces ◽  
Cellular Mechanics ◽  
Novel Technologies ◽  
Endometrial Epithelial Cell

The human endometrium is characterized by exceptional plasticity, as evidenced by rapid growth and differentiation during the menstrual cycle and fast tissue remodeling during early pregnancy. Past work has rarely addressed the role of cellular mechanics in these processes. It is becoming increasingly clear that sensing and responding to mechanical forces are as significant for cell behavior as biochemical signaling. Here, we provide an overview of experimental evidence and concepts that illustrate how mechanical forces influence endometrial cell behavior during the hormone-driven menstrual cycle and prepare the endometrium for embryo implantation. Given the fundamental species differences during implantation, we restrict the review to the human situation. Novel technologies and devices such as 3D multifrequency magnetic resonance elastography, atomic force microscopy, organ-on-a-chip microfluidic systems, stem-cell-derived organoid formation, and complex 3D co-culture systems have propelled the understanding how endometrial receptivity and blastocyst implantation are regulated in the human uterus. Accumulating evidence has shown that junctional adhesion, cytoskeletal rearrangement, and extracellular matrix stiffness affect the local force balance that regulates endometrial differentiation and blastocyst invasion. A focus of this review is on the hormonal regulation of endometrial epithelial cell mechanics. We discuss potential implications for embryo implantation.

Interleukin-18 system messenger RNA and protein expression in human endometrium during the menstrual cycle

2006 ◽  
Vol 86 (4) ◽  
pp. 905-913 ◽  
Author(s):  
H HUANG ◽  
S CHAN ◽  
H YU ◽  
H WANG ◽  
C LAI ◽  
...  
Keyword(s):  
Menstrual Cycle ◽  
Protein Expression ◽  
Messenger Rna ◽  
Human Endometrium ◽  
Interleukin 18

Single-cell transcriptomic atlas of the human endometrium during the menstrual cycle

2020 ◽  
Vol 26 (10) ◽  
pp. 1644-1653 ◽  
Author(s):  
Wanxin Wang ◽  
Felipe Vilella ◽  
Pilar Alama ◽  
Inmaculada Moreno ◽  
Marco Mignardi ◽  
...  
Keyword(s):  
Menstrual Cycle ◽  
Single Cell ◽  
Human Endometrium

scholarly journals Cloning and Expression Analysis of Two Kdm Lysine Demethylases in the Testes of Mature Yaks and Their Sterile Hybrids

Animals ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 521
Author(s):  
Zhenhua Shen ◽  
Lin Huang ◽  
Suyu Jin ◽  
Yucai Zheng
Keyword(s):  
Amino Acids ◽  
Protein Expression ◽  
Male Sterility ◽  
Histone Methylation ◽  
Real Time Pcr ◽  
Cloning And Expression ◽  
Rt Pcr ◽  
Coding Sequences ◽  
Mrna And Protein Expression ◽  
Sterile Hybrids

The objective of this study was to explore the molecular mechanism for male sterility of yak hybrids based on two demethylases. Total RNA was extracted from the testes of adult yaks (n = 10) and yak hybrids (cattle–yaks, n = 10). The coding sequences (CDS) of two lysine demethylases (KDMs), KDM1A and KDM4B, were cloned by RT-PCR. The levels of KDM1A and KDM4B in yaks and cattle–yaks testes were detected using Real-time PCR and Western blotting for mRNA and protein, respectively. In addition, the histone methylation modifications of H3K36me3 and H3K27me3 were compared between testes of yaks and cattle–yaks using ELISA. The CDS of KDM1A and KDM4B were obtained from yak testes. The results showed that the CDS of KDM1A exhibited two variants: variant 1 has a CDS of 2622 bp, encoding 873 amino acids, while variant 2 has a CDS of 2562 bp, encoding 853 amino acids. The CDS of the KDM4B gene was 3351 bp in length, encoding 1116 amino acids. The mRNA and protein expression of KDM1A and KDM4B, as well as the level of H3K36me3, were dramatically decreased in the testes of cattle–yaks compared with yaks. The present results suggest that the male sterility of cattle–yaks might be associated with reduced histone methylation modifications.

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