scholarly journals PREGNANT MARE SERUM GONADOTROPHIN: RATE OF CLEARANCE FROM THE CIRCULATION OF SHEEP

Reproduction ◽  
1975 ◽  
Vol 44 (1) ◽  
pp. 95-100 ◽  
Author(s):  
J. E. A. McINTOSH ◽  
R. M. MOOR ◽  
W. R. ALLEN
Keyword(s):  
Pregnant Mare Serum Gonadotrophin

scholarly journals Improving the Quality of Oocytes of Old and Productive Ages White Rats (Rattus norvegicus) Using Pregnant Mare Serum Gonadotrophin

2019 ◽  
Vol 3 (1) ◽  
Author(s):  
Sultania A Suleman ◽  
Andriyanto Andriyanto ◽  
Aulia Andi Mustika ◽  
Wasmen Manalu
Keyword(s):  
Rattus Norvegicus ◽  
Oocyte Quality ◽  
The Other ◽  
Female Rats ◽  
Age Group ◽  
White Rats ◽  
Maze Test ◽  
Swimming Test ◽  
Pregnant Mare Serum Gonadotrophin

Abstract This study was conducted to improve the quality of oocytes in old-female rats by using pregnant mare serum gonadotrophin (PMSG). Female rats at productive age were used as a control.  The experimental rats were injected with 4 doses of PMSG i.e., 0, 2.5, 5.0, and 10 IU PMSG. After 2 weeks of acclimation to the experimantal condition, the experimental rats were injected with PGF2α at a dose of 25µg/g BW two times with 2 days interval to synchronize estrous cycle. PMSG injections were conducted at the same time with the second PGF2α injection.  After PMSG injection, the experimental rats were divided into two groups rats, i.e., rats without mating for masurement of oocyte qualities and rats mated for measurement of offspring qualities.  Therefore, 16 experimental rats from each age group were sacrifized for maeasurement of oocyte qualities.  The other 16 experimental rats for each age group were mated for measurement of offspring qualities. Parameters measured were hematological profile, uterine and ovarian weights, the qualities of oocytes, the qualities of the offsspring born by using swimming test and rat maze test. The collected data were analyzed by analysis of variance (ANOVA) and continued with Duncan test with a 95% confidence level. The data were analyzed using SSPS. The results showed that the improvement in the quality of oocytes in old female rats using the PMSG hormone in this study showed an increase in the quality of oocytes in old and productive age rats. The highest number of oocyte quality was found in rats of productive age (3.25) and significantly different (P< 0.05) from the other oocyte qualities. The quality of offspring born to old age and productive age rats injected with PMSG were improved.  It was concluded that the improvement of oocyte quality by using PMSG also improves offspring qualities.

The effect of the laparoscopic insemination technique on the oestrous cycle of the ewe

1991 ◽  
Vol 62 (2) ◽  
pp. 60-61
Author(s):  
T. L. Taljaard ◽  
S. J. Terblanche ◽  
H. J. Bertschinger ◽  
L. J. Van Vuuren
Keyword(s):  
Cycle Length ◽  
Intrauterine Insemination ◽  
Uterine Horn ◽  
Oestrous Cycle ◽  
Control Group ◽  
Control Groups ◽  
The Mean ◽  
And Control ◽  
Pregnant Mare Serum Gonadotrophin ◽  
Teaser Rams

This investigation was designed to determine whether or not the technique of intrauterine insemination affects the length of the subsequent oestrous cycle. Dorper ewes (n=31) were divided into treatment and control groups. All the ewes were synchronised using 40 mg fluorogestone acetate intravaginal sponges for 14 d and 300 IU pregnant mare serum gonadotrophin on the day of sponge removal. A standard semen diluent was deposited laparoscopically in each uterine horn of ewes in the treatment, group. Teaser rams were used to detect oestrus. Progesterone profiles were used to confirm oestrus. The mean oestrous cycle length of 17,83 ± 0,69 d for the group in which the diluent was deposited by laparoscopy did not differ significantly (P0,l) from the 18,36±2,11 d of the control group. The technique of laparoscopic insemination did not influence the length of subsequent oestrous cycles.

Field Trials in Fixed Time Sheep A.I. in Ireland

1984 ◽  
Vol 1984 ◽  
pp. 99-99
Author(s):  
T F Crosby ◽  
M P Boland ◽  
I Gordon
Keyword(s):  
Wave Motion ◽  
Day Treatment ◽  
Skim Milk ◽  
Fixed Time ◽  
Field Trials ◽  
Single Intramuscular Injection ◽  
Previously Treated ◽  
Breeding Seasons ◽  
Natural Service ◽  
Pregnant Mare Serum Gonadotrophin

Studies reported from this laboratory in the mid seventies showed that it was possible to achieve a conception rate of 73% to a single fixed-time insemination in the autumn breeding season in ewes previously treated with intravaginal progestagen and pregnant mare serum gonadotrophin (PMSG). The present work was aimed principally at an examination of the AI technique in commercial flocks when applied over a period of three years (1979–81) and when applied in sheep of different breeds (Galways and Suffolk-cross).In the course of three successive breeding seasons, a total of 2,704 cyclic ewes in 87 farm flocks were bred to Texel or Suffolk rams by A.I. using a sperm dose of 400 million in 0.2 ml volume of a skim milk diluent; only semen samples showing a wave motion rating of 3.5 or higher were used and accepted samples were pooled. A further 525 ewes in 20 farm flocks were bred by natural service (ratio of 1 ram: 10 ewes) in one area covered by the AI work. Control of oestrus and ovulation was achieved by a 12-day treatment using commercially available progestagen sponges (Chronogest, Intervet. Ltd.; Veramix, Upjohn Ltd.); at sponge withdrawal a single intramuscular injection of 500 i.u. PMSG (Intervet. Ltd.) was administered.

ANTERIOR PITUITARY SENSITIVITY IN IMMATURE FEMALE RATS STIMULATED WITH GONADOTROPHIN RELEASING HORMONE IN VIVO: EFFECT OF PRIMING WITH OESTROGEN, PREGNANT MARE SERUM GONADOTROPHIN OR BRAIN LESION

1977 ◽  
Vol 74 (1) ◽  
pp. 99-109 ◽  
Author(s):  
D. DE ZIEGLER ◽  
M. WILKINSON ◽  
DANIELLE CASSARD ◽  
K. B. RUF
Keyword(s):  
Brain Lesion ◽  
Treated Group ◽  
Female Rats ◽  
Similar Degree ◽  
Female Rat ◽  
Immature Female ◽  
Releasing Hormone ◽  
Gonadotrophin Releasing Hormone ◽  
Pregnant Mare Serum Gonadotrophin

An investigation of pituitary sensitivity, assessed in terms of increments in plasma LH and FSH concentrations, to stimulation with one or two injections of gonadotrophin releasing hormone (GnRH) was carried out on 26-day-old immature female rats which had received one of the following priming treatments: 10 μg oestradiol benzoate (OB) as a single injection on day 23 or day 25, or on both days; 10 i.u. pregnant mare serum gonadotrophin (PMSG) on day 24; an electrochemical brain lesion placed in the mediobasal hypothalamus on day 23; control animals received either vehicle alone or a sham lesion. Pituitary sensitivity assessed at 10.00 h on day 26, after one or two injections of GnRH (100 ng/100 g body weight, s.c.), was enhanced to a similar degree in the three groups treated with OB in terms of LH (P < 0-01). The FSH response also increased after OB treatment but was not statistically significant. In contrast, 48 h after the injection of PMSG (i.e. when the rats were in a 'pro-oestrous-like' condition) pituitary sensitivity in terms of both LH and FSH dropped sharply (P < 0·001). In lesioned animals, pituitary sensitivity to one injection of GnRH was unchanged. A second GnRH injection administered after a 60 min interval induced a slightly larger LH response in control animals. In contrast, the ratio of the second response to the first increased in animals treated with PMSG, despite the state of overall decrease in sensitivity, being 4·5:1 in PMSG-treated rats versus 1·4:1 in controls. In a second set of experiments, we investigated the variation of pituitary sensitivity in conjunction with an experimentally induced gonadotrophin surge. In animals treated with OB on day 23 and with 1 mg progesterone at 12·00 h on day 26, pituitary sensitivity was increased at both 14.00 and 17.00 h as compared with that in the day 23 OB-treated group at 10.00 h. The PMSG-treated animals maintained their state of decreased responsiveness at 14.00 h, but exhibited increased pituitary sensitivity at the time of the gonadotrophin surge (17.00 h). These results show that OB increases pituitary sensitivity to GnRH in 26-day-old female rats and that the induction of a gonadotrophin surge further increases this sensitivity. In contrast, PMSG-treated rats displayed a state of decreased responsiveness 48 and 52 h, but not 55 h, after the injection. Pituitary sensitivity on the second day after PMSG treatment thus clearly differs from that observed during pro-oestrus in the adult cyclic female rat.

STEROIDAL CONTROL OF THE RELEASE OF THE PREOVULATORY SURGE OF LUTEINIZING HORMONE IN THE RAT

1978 ◽  
Vol 79 (2) ◽  
pp. 223-234 ◽  
Author(s):  
CATHERINE A. WILSON ◽  
J. C. HADLEY ◽  
D. GILBERT ◽  
A. S. McNEILLY
Keyword(s):  
Plasma Concentration ◽  
Luteinizing Hormone ◽  
Critical Period ◽  
Adrenal Glands ◽  
Sham Operation ◽  
Late Afternoon ◽  
Immature Rats ◽  
Pregnant Mare Serum Gonadotrophin ◽  
Adrenalectomized Rats

Experiments were carried out on 4 day cyclic rats or immature rats induced to ovulate by administration of pregnant mare serum gonadotrophin. Removal of the ovaries and adrenal glands at 17.00 h of pro-oestrus, i.e. after the critical period, prevented the appearance of the surge of LH. Sham-operation or removal of only one of the sets of glands had no effect. This indicates that the preovulatory increase in the concentration of oestradiol is not solely responsible for the surge of LH; the presence of a steroid, secreted by the ovaries and adrenal glands in the late afternoon of pro-oestrus, is also required. Attempts were made to reinstate the surge of LH in ovariectomized, adrenalectomized rats by administration of one of the steroids normally secreted in late pro-oestrus. Corticosterone, 20α- and 20β-hydroxy-4-pregnen-3-one and 17α-hydroxyprogesterone all had no effect. Progesterone injected at the time of the operation stimulated the release of LH but only after the plasma concentration had reached its maximum 3–5 h after injection. Testosterone also stimulated the release of LH some hours after administration.

Internalization of receptor-bound human chorionic gonadotrophin in preovulatory rat granulosa cells in vivo

1983 ◽  
Vol 103 (3) ◽  
pp. 406-412 ◽  
Author(s):  
Kalle Jääkeläinen ◽  
Seppo Markkanen ◽  
Hannu Rajaniemi
Keyword(s):  
Plasma Membrane ◽  
Granulosa Cells ◽  
Subcellular Distribution ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Female Rats ◽  
Electron Microscope Autoradiography ◽  
Silver Grains ◽  
Pregnant Mare Serum Gonadotrophin

Abstract. The subcellular distribution of 125I-labelled human chorionic gonadotrophin (hCG) in preovulatory rat granulosa cells was studied in vivo. Pregnant mare serum gonadotrophin-pretreated immature female rats received an iv injection of [125I]hCG a few hours before the endogenous preovulatory gonadotrophin surge. The animals were killed at 2 or 6 h after the [125I]hCG injections. Light microscope autoradiographs showed that the mural granulosa cells of large follicles were the most highly labelled cells in the ovaries. Electron microscope autoradiography was used to study the subcellular distribution of radioactivity in the mural granulosa cells. At 2 h 45% of the counted silver grains were associated with the plasma membrane and 10% with the lysosomes, at 6 h the values were 51% and 9%, respectively. The distribution of the observed silver grains was compared with the generated expected source to grain pairs by computerized linear multiple regression analysis. The magnitudes of the regression coefficients revealed that the plasma membrane and the lysosomes were the only specifically 125I-labelled organelles, that a few radioactive molecules were located diffusely over the cytoplasm at 2 h and that the 125I-radioactivity of the nuclei was negligible. The present results suggest that preovulatory rat granulosa cells are in vivo able to internalize into lysosomes [125I]hCG initially bound to LH/hCG receptors of the plasma membrane.

Effects of thymulin on spontaneous puberty and gonadotrophin-induced ovulation in prepubertal normal and hypothymic mice

1999 ◽  
Vol 163 (2) ◽  
pp. 255-260 ◽  
Author(s):  
L Hinojosa ◽  
R Chavira ◽  
R Dominguez ◽  
P Rosas
Keyword(s):  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Vaginal Opening ◽  
Serum Progesterone ◽  
Thymic Hormone ◽  
Induced Ovulation ◽  
Gonadotrophin Secretion ◽  
Age Dependent ◽  
Neuroendocrine Mechanisms ◽  
Pregnant Mare Serum Gonadotrophin

The effects of thymulin administration beginning on days 19 or 24 of age on spontaneous puberty and gonadotrophin-induced ovulation were analysed in female normal and hypothymic mice. In normal and hypothymic mice, the daily administration of thymulin at 24 days of age resulted in a delay in the age of vaginal opening, with an increase in serum progesterone levels. Normal mice treated with 200 ng thymulin beginning on day 19 of age and injected with pregnant mare serum gonadotrophin (PMSG) 24 h later had an increase in ovulation rate, number of ova shed and weight of the ovaries. None of the hypothymic mice treated with thymulin on day 19 and PMSG on day 20 ovulated. PMSG treatment on day 25 induced ovulation in hypothymic mice. When these animals were injected previously with 200 ng thymulin, the number of ova shed by ovulating animals was lower than in PMSG-treated animals. Administration of thymulin and sequential injection of PMSG and human chorionic gonadotrophin 54 h later resulted in an increase in ovulatory response in comparison with those receiving only PMSG. The results suggest that thymulin plays a role in the regulation of spontaneous puberty through its effects on adrenal and ovarian endocrine functions. The increase in the ovarian PMSG response-treated animals, previously given thymulin, showed that this thymic hormone participates in the regulation of gonadotrophin secretion mechanisms and seems to be dose- and age-dependent. In hypothymic mice, neuroendocrine mechanisms regulating puberty are different from those of normal mice.

Relationship of follicle stimulating hormone (FSH)-sensitive adenylate cyclase activity to FSH binding in immature rat ovaries following administration of pregnant mare serum gonadotrophin

1982 ◽  
Vol 101 (4) ◽  
pp. 625-629 ◽  
Author(s):  
C. Y. Lee ◽  
L. E. Reichert
Keyword(s):  
Adenylate Cyclase ◽  
Dose Response ◽  
Follicle Stimulating Hormone ◽  
Optimal Dose ◽  
Cyclase Activity ◽  
Adenylate Cyclase Activity ◽  
Adenylate Cyclase System ◽  
Immature Rat ◽  
Pregnant Mare Serum Gonadotrophin ◽  
Stimulating Hormone

Abstract. The time and dose-response relationships of human follicle stimulating hormone (hFSH)-sensitive adenylate cyclase activity to hFSH binding was studied in the immature rat ovary following an sc injection of pregnant mare serum gonadotrophin (PMSG). When an optimal dose of PMSG (10 IU/rat) was administered, a marked increase in hFSH-sensitive activity was observed at day 2, followed by a sharp decline at day 3. This was accompanied by a parallel rise and fall in ovarian hFSH binding activity. When immature rats were given various doses (5–100 IU/rat) of PMSG for 2 days, hFSH-sensitive adenylate cyclase activity increased sharply and maximal stimulation was obtained at 10 IU/rat. A close correlation was also observed with respect to dose-response for hCG-sensitive adenylate cyclase and hCG binding activities. It is concluded that: 1) PMSG administration with an optimal dose to the immature rat induced ovarian FSH and LH-hCG receptors, and an adenylate cyclase system highly sensitive to hFSH and hCG, and 2) the acquisition and responsiveness of adenylate cylcase to gonadotrophins are closely related to the appearance and the numbers of gonadotrophin receptors.

Sign in / Sign up

Export Citation Format

Share Document