Competitive protein-binding analysis of ovine and bovine plasma progesterone

Reproduction ◽  
1972 ◽  
Vol 28 (1) ◽  
pp. 148-150 ◽  
Author(s):  
M. Cain ◽  
J. Cerini ◽  
M. Cerini ◽  
W. Chamley ◽  
I. Cumming ◽  
...  
Keyword(s):  
Protein Binding ◽  
Plasma Progesterone ◽  
Binding Analysis ◽  
Bovine Plasma ◽  
Competitive Protein Binding

FURTHER ASSESSMENT OF THE RELIABILITY OF PROGESTERONE ASSAYS BY COMPETITIVE PROTEIN BINDING

1970 ◽  
Vol 65 (1_Suppl) ◽  
pp. S171-S187 ◽  
Author(s):  
M. L. A. de Souza ◽  
H. O. Williamson ◽  
L. O. Moody ◽  
E. Diczfalusy
Keyword(s):  
Protein Binding ◽  
Purification Procedure ◽  
Displacement Effect ◽  
Plasma Progesterone ◽  
Binding Analysis ◽  
Extraction And Purification ◽  
Competitive Protein Binding ◽  
Proper Consideration

ABSTRACT Experimental variables influencing the determination of plasma progesterone by competitive protein binding analysis have been further assessed. The precision of the method has been improved by additional mechanisation. Method interfering factors introduced by the extraction and purification procedure have been evaluated quantitatively. Another type of interfering material is present in plasma extracts from which all progesterone has been removed. The displacement effect of this material is additive to that derived from solvent and chromagram residues. Unless the various types of interfering material are taken into proper consideration, competitive protein binding methods for steroids may yield erroneous results.

BOVINE PLASMA OESTROGENS, PROGESTERONE AND GLUCOCORTICOIDS DURING DEXAMETHASONE INDUCED PARTURITION

1976 ◽  
Vol 81 (2) ◽  
pp. 385-397 ◽  
Author(s):  
L. E. Evans ◽  
W. C. Wagner
Keyword(s):  
Protein Binding ◽  
Plasma Samples ◽  
Plasma Progesterone ◽  
Peripheral Plasma ◽  
Progesterone Production ◽  
Bovine Plasma ◽  
Competitive Protein Binding ◽  
Pre Treatment

ABSTRACT Plasma samples were collected from jugular, uterine and utero-ovarian veins during glucocorticoid induced parturition. Plasma oestrogens, corticosteroids and progesterone were determined by competitive protein binding methods. Corticosteroids and progesterone began to decline within 8 to 10 h following DXMS treatment. Corticoids were only temporarily suppressed, while progesterone fell to minimum levels and remained low through calving. At this stage of gestation (270 days) peripheral plasma progesterone was primarily of ovarian origin. Pre-treatment with HCG appeared to support progesterone production by the CL despite DXMS treatment in 2 of 6 cows. These 2 cows failed to calve within the expected 96 h after DXMS. Plasma oestrogens did not show significant increases until 24 h after DXMS treatment. Cows which responded to DXMS treatment (calved) had significantly higher oestrogen levels than those which did not respond. It was concluded that oestrogens probably play a permissive rather than an initiating role in parturition.

EVALUATION OF A SIMPLIFIED METHOD FOR DETERMINING SERUM THYROXINE BY COMPETITIVE PROTEIN BINDING ANALYSIS

1970 ◽  
Vol 64 (4) ◽  
pp. 630-636 ◽  
Author(s):  
Stephen C. Thorson ◽  
Ronald Tsujikawa ◽  
James L. Brown ◽  
Robert T. Morrison ◽  
Hamish W. McIntosh
Keyword(s):  
Protein Binding ◽  
Clinical Application ◽  
Thyroid Status ◽  
Serum Thyroxine ◽  
Binding Analysis ◽  
Simplified Method ◽  
Competitive Protein Binding ◽  
Hypothyroid Patients

ABSTRACT Serum thyroxine concentrations were determined in 66 euthyroid, 30 hyperthyroid and 13 hypothyroid patients using both the established Murphy method and a simplified method of competitive protein binding analysis. A diagnosis compatibility of 96% was found with both methods indicating that the simplified method has comparable clinical application as an initial screen of thyroid status.

AN IMPROVED METHOD FOR THE ASSAY OF PROGESTERONE BY COMPETITIVE PROTEIN BINDING

1970 ◽  
Vol 63 (2) ◽  
pp. 225-241 ◽  
Author(s):  
B. D. Reeves ◽  
M. L. A. de Souza ◽  
I. E. Thompson ◽  
E. Diczfalusy
Keyword(s):  
Protein Binding ◽  
Binding Sites ◽  
Entire Range ◽  
Improved Method ◽  
Plasma Progesterone ◽  
Extraction And Purification ◽  
Corticosteroid Binding Globulin ◽  
Competitive Protein Binding ◽  
The Individual ◽  
Range Of Values

ABSTRACT An improved method for the assay of plasma progesterone by competitive protein binding is described. The improvement is based upon rigorous control of the variables, the compensation for and standardisation of interfering factors inherent in the method and the use of a human corticosteroid binding globulin, that meets the requirements for sensitivity at levels of 1.0 ng of progesterone and below. The assessment of the reliability of the individual steps in the method as well as that of the complete method is presented. The sensitivity of the method is around 0.2 ng progesterone per ml plasma. Accuracy was measured by adding progesterone in amounts ranging from 0.0 to 1.0 ng to 1.0 ml plasma. There was a linear relationship between the progesterone added and recovered throughout the entire range of values, with a coefficient of correlation (r) of 0.94. Of 52 related steroids tested, none was found which would remain associated with progesterone following extraction and purification and which would also compete with progesterone for binding sites.

Measurement of Plasma Testosterone by Means of Competitive Protein Binding Analysis

1969 ◽  
Vol 29 (6) ◽  
pp. 854-859 ◽  
Author(s):  
ROBERT L. ROSENFIELD ◽  
WALTER R. EBERLEIN ◽  
ALFRED M. BONGIOVANNI
Keyword(s):  
Protein Binding ◽  
Plasma Testosterone ◽  
Binding Analysis ◽  
Competitive Protein Binding

EVALUATION OF A RAPID METHOD FOR THE MEASUREMENT OF PLASMA PROGESTERONE BY COMPETITIVE PROTEIN BINDING

1970 ◽  
Vol 46 (3) ◽  
pp. 369-377 ◽  
Author(s):  
B. T. MARTIN ◽  
B. A. COOKE ◽  
W. P. BLACK
Keyword(s):  
Menstrual Cycle ◽  
Protein Binding ◽  
Ethyl Acetate ◽  
Rapid Method ◽  
Chromatographic Separation ◽  
Good Accuracy ◽  
Human Subjects ◽  
Plasma Progesterone ◽  
Competitive Protein Binding ◽  
Paper Chromatographic Separation

SUMMARY A competitive protein-binding method for the measurement of progesterone in plasma of human subjects was investigated. The purification steps necessary to achieve good accuracy, precision and specificity were determined. It was found that one paper chromatographic separation of unwashed ethyl acetate plasma extracts was sufficient, providing that the sample contains a minimum of 1 ng. progesterone. Water blank values equivalent to 0·05 ng. progesterone were consistently obtained. The concentrations of progesterone found in plasma during the follicular and luteal phases of the menstrual cycle and in male plasma were 0·14 ± 0·14, 0·82 ± 0·74 and 0·022 ± 0·015 (s.d.) μg./100 ml., respectively.

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