scholarly journals COMPETITIVE PROTEIN-BINDING ASSAY OF PROGESTERONE IN BOVINE JUGULAR VENOUS PLASMA DURING THE OESTROUS CYCLE

Reproduction ◽  
1971 ◽  
Vol 26 (2) ◽  
pp. 167-174 ◽  
Author(s):  
M. SHEMESH ◽  
H. R. LINDNER ◽  
N. AYALON
Keyword(s):  
Protein Binding ◽  
Binding Assay ◽  
Oestrous Cycle ◽  
Protein Binding Assay ◽  
Competitive Protein Binding Assay ◽  
Competitive Protein Binding ◽  
Venous Plasma

scholarly journals Hormone Studies on Ewes Grazing an Oestrogenic (Yarloop Clover) Pasture During the Reproductive Cycle

1975 ◽  
Vol 28 (3) ◽  
pp. 279 ◽  
Author(s):  
JM Obst ◽  
RF Seamark
Keyword(s):  
Protein Binding ◽  
Reproductive Cycle ◽  
Binding Assay ◽  
Oestrous Cycle ◽  
Endocrine Function ◽  
Plasma Progesterone ◽  
Protein Binding Assay ◽  
Competitive Protein Binding Assay ◽  
Competitive Protein Binding ◽  
Clover Pasture

The endocrine function of Merino and Corriedale ewes grazing an oestrogenic (Yarloop clover) pasture has been studied during the oestrous cycle, pregnancy and parturition, and the results compared with those from a study of similar ewes grazing a neighbouring grass pasture. Plasma progesterone, oestrogen and corticoids were measured using competitive protein binding assay procedures.

DIHYDROTESTOSTERONE IN HUMAN SPERMATIC VENOUS PLASMA

1974 ◽  
Vol 76 (2) ◽  
pp. 388-391 ◽  
Author(s):  
M. Pazzagli ◽  
D. Borrelli ◽  
G. Forti ◽  
M. Serio
Keyword(s):  
Protein Binding ◽  
Binding Assay ◽  
Venous Blood ◽  
Spermatic Vein ◽  
Human Testis ◽  
Peripheral Venous Blood ◽  
Protein Binding Assay ◽  
Competitive Protein Binding Assay ◽  
Competitive Protein Binding ◽  
Venous Plasma

ABSTRACT Testosterone (T) and 5α-dihydrotestosterone (DHT) concentrations in spermatic and peripheral venous blood obtained from six men have been measured by radioimmunoassay and competitive protein binding assay respectively. The concentrations (mean ± sd) of DHT in the spermatic vein (0.64 ± 0.33 μg/100 ml) were higher than that found in the cubital vein (0.025 ± 0.01 μg/100 ml). These results demonstrate the production of DHT by the human testis.

A Simplified competitive protein-binding assay for 25-hydroxycalciferol

1976 ◽  
Vol 68 (2) ◽  
pp. 99-105 ◽  
Author(s):  
B. Garcia-Pascual ◽  
A. Peytremann ◽  
B. Courvoisier ◽  
D.E.M. Lawson
Keyword(s):  
Protein Binding ◽  
Binding Assay ◽  
Protein Binding Assay ◽  
Competitive Protein Binding Assay ◽  
Competitive Protein Binding

A Competitive Protein-Binding Assay for 25-Hydroxycholecalciferol

1975 ◽  
Vol 17 (2) ◽  
pp. 57-57
Author(s):  
Yoshiki Seino ◽  
Tsunesuke Shimotsuji ◽  
Shintaro Okada ◽  
Teisuke Hiejima ◽  
Chiiko Ikehara ◽  
...  
Keyword(s):  
Protein Binding ◽  
Binding Assay ◽  
Protein Binding Assay ◽  
Competitive Protein Binding Assay ◽  
Competitive Protein Binding ◽  
25 Hydroxycholecalciferol

Competitive Protein-Binding Assay for 25-Hydroxycholecalciferol

1974 ◽  
Vol 46 (2) ◽  
pp. 231-240 ◽  
Author(s):  
S. Edelstein ◽  
M. Charman ◽  
D. E. M. Lawson ◽  
E. Kodicek
Keyword(s):  
Protein Binding ◽  
Binding Assay ◽  
Rat Serum ◽  
Male And Female ◽  
Normal Volunteers ◽  
Protein Binding Assay ◽  
Competitive Protein Binding Assay ◽  
Competitive Protein Binding ◽  
The Mean ◽  
25 Hydroxycholecalciferol

1. A competitive protein-binding assay for plasma 25-hydroxycholecalciferol was developed, using Sephadex LH-20 columns for the isolation of the sterol and a partially purified protein from rat serum as the binding protein. 2. The mean plasma 25-hydroxycholecalciferol in eighteen male and female normal volunteers was 38 ± SD 14 pmol/ml (15–2 ± 5–6 ng/ml). 3. Levels of 25-hydroxycholecalciferol in plasma of four male volunteers maintained for 2 months on a diet supplemented with 26·1 nmol (10 μg) of cholecalciferol a day were found to be significantly higher. 4. High correlation was established between plasma 25-hydroxycholecalciferol values obtained by the competitive protein-binding assay and values obtained by bioassay.

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