scholarly journals A xenograft and cell line model of SDH-deficient pheochromocytoma derived from Sdhb+/− rats

2020 ◽  
Vol 27 (10) ◽  
pp. X9-X10
Author(s):  
James F Powers ◽  
Brent Cochran ◽  
James D Baleja ◽  
Hadley D Sikes ◽  
Andrew D Pattison ◽  
...  
Keyword(s):  
Cell Line ◽  
Line Model ◽  
Cell Line Model

scholarly journals Adaptation to G93Asuperoxide dismutase 1 in a motor neuron cell line model of amyotrophic lateral sclerosis

FEBS Journal ◽  
2009 ◽  
Vol 276 (10) ◽  
pp. 2861-2874 ◽  
Author(s):  
Silvia Tartari ◽  
Giuseppina D’Alessandro ◽  
Elisabetta Babetto ◽  
Milena Rizzardini ◽  
Laura Conforti ◽  
...  
Keyword(s):  
Amyotrophic Lateral Sclerosis ◽  
Motor Neuron ◽  
Cell Line ◽  
Line Model ◽  
Cell Line Model ◽  
Lateral Sclerosis

A cell line model of mucin biosynthesis in the human intestine

1996 ◽  
Vol 48 (1) ◽  
pp. A36-A37
Author(s):  
B VANKLINKEN ◽  
E OUSSOREN ◽  
J WEENINK ◽  
H BULLER ◽  
J DEKKER ◽  
...  
Keyword(s):  
Cell Line ◽  
Human Intestine ◽  
Line Model ◽  
Cell Line Model ◽  
A Cell

scholarly journals Activity of EGFR, mTOR and PI3K inhibitors in an isogenic breast cell line model

2014 ◽  
Vol 7 (1) ◽  
pp. 397 ◽  
Author(s):  
Sharon Glaysher ◽  
Louise M Bolton ◽  
Penny Johnson ◽  
Christopher Torrance ◽  
Ian A Cree
Keyword(s):  
Cell Line ◽  
Breast Cell Line ◽  
Line Model ◽  
Pi3k Inhibitors ◽  
Cell Line Model

Characterization of Alternatively Spliced Isoforms of MUC4 and ADAM12 Genes in a Metastatic Colorectal Cancer Cell Line Model

2021 ◽  
Author(s):  
Saleh Althenayyan ◽  
Mohammed H AlMuhanna ◽  
Abdulkareem Al Abdulrahman ◽  
Bandar Alghanem ◽  
Suliman A. Alsagaby ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Metastatic Colorectal Cancer ◽  
Cell Line ◽  
Differential Expression ◽  
Cell Lines ◽  
Cancer Cell ◽  
Colorectal Cancer Cell ◽  
Line Model ◽  
Cell Line Model ◽  
Alternatively Spliced

Colorectal cancer prognosis get worse with advancement of disease into metastatic stage. There is a pertinent need to develop prognostic biomarkers that can be used for personalized and precision medicine. Alternative splicing provides an insight into understanding of changes at isoform expression level which may not be evident at gene level. In this direction, we utilized our prior knowledge about significant alternatively spliced genes and chose ADAM12 and MUC4 for further characterization in a metastatic cell line model. These genes were found to be good prognostic indicators in The Cancer Genome Atlas database. We studied the gene organization and designed primers to specifically amplify a group of isoforms. Differential expression of these group of isoforms was observed in normal, primary and metastatic colorectal cancer cell lines. We further validated the results using sanger sequencing. Isoform expression was found to respond to the 5-fluorouracil treatment. RNAseq analysis of the cell lines further validated the differential expression of gene isoforms. Successful detection of ADAM12 and MUC4 in cell lysates varied according to the antibody used which may reflect differential expression of isoforms. This comprehensive study underscores the importance of studying alternatively spliced isoforms and their probable used as prognostic or predictive biomarkers.

scholarly journals Dysregulation of the IFN-γ-STAT1 signaling pathway in a cell line model of large granular lymphocyte leukemia

PLoS ONE ◽  
2018 ◽  
Vol 13 (2) ◽  
pp. e0193429 ◽  
Author(s):  
Paige M. Kulling ◽  
Kristine C. Olson ◽  
Cait E. Hamele ◽  
Mariella F. Toro ◽  
Su-Fern Tan ◽  
...  
Keyword(s):  
Cell Line ◽  
Signaling Pathway ◽  
Large Granular Lymphocyte ◽  
Line Model ◽  
Large Granular Lymphocyte Leukemia ◽  
Cell Line Model ◽  
A Cell ◽  
Stat1 Signaling ◽  
Ifn Γ

In vitro antioxidant activities of Rhodobacter sphaeroides and protective effect on Caco-2 cell line model

2018 ◽  
Vol 103 (2) ◽  
pp. 917-927 ◽  
Author(s):  
Jun An ◽  
Cui Yang ◽  
Zuming Li ◽  
Patricia W. Finn ◽  
David L. Perkins ◽  
...  
Keyword(s):  
Protective Effect ◽  
Cell Line ◽  
Rhodobacter Sphaeroides ◽  
Antioxidant Activities ◽  
Line Model ◽  
Cell Line Model

scholarly journals A Customisable Cell Line Model of Glioblastoma

2021 ◽  
Author(s):  
◽  
Devlin Forsythe
Keyword(s):  
Metabolic Rate ◽  
Cell Line ◽  
Cell Lines ◽  
Survival Rates ◽  
Future Research ◽  
Line Model ◽  
Malignant Brain Tumour ◽  
Cell Line Model ◽  
Prognosis Research ◽  
Transplantation Model

<p><b>Glioblastoma is an extremely malignant brain tumour with one of the lowest survival rates of all cancers. Current treatments do very little to alter this prognosis. Research into new therapies and the biology of glioblastoma has made scarce progress over the past decades. This is partly due to the combination of the tumour’s heterogeneity, and the inability of the current animal models to accurately depict this. This project was a pilot study into the development and characterisation of a novel cell line model of glioblastoma, which could be transplanted into immune competent mice, in order to study the disease.</b></p> <p>An immortalised C57BL/6 astrocyte cell line, with an EGFP transgene, was used as the base to add glioblastoma specific mutations. To produce a ‘classical-like’ glioblastoma model, a knockout in Pten was induced, onto which two separate lines the human oncogenes, EGFRVIII and RAS V12, were stably expressed. ‘Secondary-like’ models were created with a knockout of P53, and the stable transfection of IDH1R132H.</p> <p>The ‘classical-like’ cell lines were assessed for how well they mimicked a classical glioblastoma. The Pten knockout cell line showed an increased proliferative and metabolic rate compared with the astrocytes and a significant increase in clonogenicity. The addition of RAS V12 to the cells showed an increased migratory capacity; and the Pten + EGFRVIII cell line had a tendency towards an increased proliferation. The ‘secondary-like’ cell lines were assessed for their survival-related phenotypes. The P53 cell line showed a decreased proliferative rate, but with an increased metabolic rate and clonogenic ability. The introduction of the IDH1 mutant protein resulted in a decreased rate of G2 arrest in response to ionising radiation.</p> <p>These cell lines recapitulated what is seen in human glioblastoma tumours and show potential as a transplantation model. Future research will investigate the tumorigenicity of these cell lines.</p>

BIOAVAILABILITY OF BERYLLIUM OXIDE PARTICLES: AN IN VITRO STUDY IN THE MURINE J774A.1 MACROPHAGE CELL LINE MODEL

2005 ◽  
Vol 31 (3) ◽  
pp. 341-360 ◽  
Author(s):  
Gregory A. Day ◽  
Mark D. Hoover ◽  
Aleksandr B. Stefaniak ◽  
Robert M. Dickerson ◽  
Eric J. Peterson ◽  
...  
Keyword(s):  
Cell Line ◽  
In Vitro Study ◽  
Beryllium Oxide ◽  
Vitro Study ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Line Model ◽  
Cell Line Model ◽  
Oxide Particles
Sign in / Sign up

Export Citation Format

Share Document