scholarly journals Magnetic resonance spectroscopy of paragangliomas: new insights into in vivo metabolomics

2015 ◽  
Vol 22 (4) ◽  
pp. M1-M8 ◽  
Author(s):  
Arthur Varoquaux ◽  
Yann le Fur ◽  
Alessio Imperiale ◽  
Antony Reyre ◽  
Marion Montava ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Ex Vivo ◽  
Tca Cycle ◽  
Resonance Spectroscopy ◽  
Variants Of Unknown Significance ◽  
Neck Tumors ◽  
Single Voxel ◽  
Unknown Significance ◽  
Genetically Determined

Paragangliomas (PGLs) can be associated with mutations in genes of the tricarboxylic acid (TCA) cycle. Succinate dehydrogenase (SDHx) mutations are the prime examples of genetically determined TCA cycle defects with accumulation of succinate. Succinate, which acts as an oncometabolite, can be detected by ex vivo metabolomics approaches. The aim of this study was to evaluate the potential role of proton magnetic resonance (MR) spectroscopy (1H-MRS) for identifying SDHx-related PGLs in vivo and noninvasively. Eight patients were prospectively evaluated with single voxel 1H-MRS. MR spectra from eight tumors (four SDHx-related PGLs, two sporadic PGLs, one cervical schwannoma, and one cervical neurofibroma) were acquired and interpreted qualitatively. Compared to other tumors, a succinate resonance peak was detected only in SDHx-related tumor patients. Spectra quality was considered good in three cases, medium in two cases, poor in two cases, and uninterpretable in the latter case. Smaller lesions had lower spectra quality compared to larger lesions. Jugular PGLs also exhibited a poorer spectra quality compared to other locations. 1H-MRS has always been challenging in terms of its technical requisites. This is even more true for the evaluation of head and neck tumors. However, 1H-MRS might be added to the classical MR sequences for metabolomic characterization of PGLs. In vivo detection of succinate might guide genetic testing, characterize SDHx variants of unknown significance (in the absence of available tumor sample), and even optimize a selection of appropriate therapies.

scholarly journals Proton Magnetic Resonance Spectroscopy Characterization of Rathke’s Cleft Cysts (RCCs): Relevance to the Differential Diagnosis of Pituitary Adenomas and RCCs

Cancers ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 360
Author(s):  
Omkar B. Ijare ◽  
Martyn A. Sharpe ◽  
David S. Baskin ◽  
Kumar Pichumani
Keyword(s):  
Differential Diagnosis ◽  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Pituitary Adenomas ◽  
Ex Vivo ◽  
Medical Professionals ◽  
Resonance Spectroscopy ◽  
Rathke's Cleft Cysts ◽  
Rathke's Cleft

Background: Rathke’s Cleft Cysts (RCCs) are rare epithelial cysts arising from remnants of the Rathke pouch in the pituitary gland. A subset of these lesions enlarge and produce a mass effect with consequent hypopituitarism, and may result in visual loss. Moreover, some RCCs with a high intra-cystic protein content may mimic cystic pituitary adenoma, which makes their differential diagnosis ambiguous. Currently, medical professionals have no definitive way to distinguish RCCs from pituitary adenomas. Therefore, preoperative confirmation of RCCs would be of help to medical professionals for the management and proper surgical decision making. The goal of this study is to identify molecular markers in RCCs. Methods: We characterized aqueous and chloroform extracts of surgically resected RCCs and pituitary adenomas using ex vivo 1H NMR spectroscopy. Results: All RCCs exclusively showed the presence of mucopolysaccharides which are glycosaminoglycans (GAGs) made up of disaccharides of aminosugars and uronic sugars. Conclusion: GAGs can be used as metabolite marker for the detection of RCCs and this knowledge will lay the groundwork for the development of a non-invasive, in vivo magnetic resonance spectroscopy methodology for the differential diagnosis of RCCs and pituitary adenomas using clinical MRI scanners.

In Vivo and Ex Vivo Magnetic Resonance Spectroscopy in the Characterization of Hemangioblastoma Cyst Fluid

2014 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Girolamo Crisi ◽  
Silvano Filice ◽  
Thelma A. Pertinhez ◽  
Elisa Ventura ◽  
Franco Servadei
Keyword(s):  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Ex Vivo ◽  
Cyst Fluid ◽  
Resonance Spectroscopy

scholarly journals Metabolic Profiling of the Rat Liver After Chronic Ingestion of Alpha-Naphthylisothiocyanate Using In Vivo and Ex Vivo Magnetic Resonance Spectroscopy

2012 ◽  
Vol 126 (2) ◽  
pp. 306-316 ◽  
Author(s):  
B. S. Solanky ◽  
G. J. Sanchez-Canon ◽  
J. F. L. Cobbold ◽  
S. D. Taylor-Robinson ◽  
J. D. Bell ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Rat Liver ◽  
Metabolic Profiling ◽  
Ex Vivo ◽  
Resonance Spectroscopy ◽  
Chronic Ingestion

scholarly journals Comparison of single-voxel 1H-cardiovascular magnetic resonance spectroscopy techniques for in vivo measurement of myocardial creatine and triglycerides at 3T

2021 ◽  
Vol 23 (1) ◽  
Author(s):  
Joevin Sourdon ◽  
Tangi Roussel ◽  
Claire Costes ◽  
Patrick Viout ◽  
Maxime Guye ◽  
...  
Keyword(s):  
Cardiovascular Magnetic Resonance ◽  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Linear Regression Analysis ◽  
Interventricular Septum ◽  
Free Breathing ◽  
Breath Hold ◽  
Resonance Spectroscopy ◽  
Single Voxel

Abstract Background Single-voxel proton cardiovascular magnetic resonance spectroscopy (1H-CMRS) benefits from 3 T to detect metabolic abnormalities with the quantification of intramyocardial fatty acids (FA) and creatine (Cr). Conventional point resolved spectroscopy (PRESS) sequence remains the preferred choice for CMRS, despite its chemical shift displacement error (CSDE) at high field (≥ 3 T). Alternative candidate sequences are the semi-adiabatic Localization by Adiabatic SElective Refocusing (sLASER) recommended for brain and musculoskeletal applications and the localized stimulated echo acquisition mode (STEAM). In this study, we aim to compare these three single-voxel 1H-CMRS techniques: PRESS, sLASER and STEAM for reproducible quantification of myocardial FA and Cr at 3 T. Sequences are compared both using breath-hold (BH) and free-breathing (FB) acquisitions. Methods CMRS accuracy and theoretical CSDE were verified on a purposely-designed fat–water phantom. FA and Cr CMRS data quality and reliability were evaluated in the interventricular septum of 10 healthy subjects, comparing repeated BH and free-breathing with retrospective gating. Results Measured FA/W ratio deviated from expected phantom ratio due to CSDE with all sequences. sLASER supplied the lowest bias (10%, vs -28% and 27% for PRESS and STEAM). In vivo, PRESS provided the highest signal-to-noise ratio (SNR) in FB scans (27.5 for Cr and 103.2 for FA). Nevertheless, a linear regression analysis between the two BH showed a better correlation between myocardial Cr content measured with sLASER compared to PRESS (r = 0.46; p = 0.03 vs. r = 0.35; p = 0.07) and similar slopes of regression lines for FA measurements (r = 0.94; p < 0.001 vs. r = 0.87; p < 0.001). STEAM was unable to perform Cr measurement and was the method with the lowest correlation (r = 0.59; p = 0.07) for FA. No difference was found between measurements done either during BH or FB for Cr, FA and triglycerides using PRESS, sLASER and STEAM. Conclusion When quantifying myocardial lipids and creatine with CMR proton spectroscopy at 3 T, PRESS provided higher SNR, while sLASER was more reproducible both with single BH and FB scans.

scholarly journals Hyperpolarized 13C pyruvate magnetic resonance spectroscopy for in vivo metabolic phenotyping of rat HCC

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Elisabeth Bliemsrieder ◽  
Georgios Kaissis ◽  
Martin Grashei ◽  
Geoffrey Topping ◽  
Jennifer Altomonte ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Tumor Tissue ◽  
Ex Vivo ◽  
Kinetic Modelling ◽  
Lactate Production ◽  
Resonance Spectroscopy ◽  
Primary Tumors ◽  
Conversion Rates

AbstractThe in vivo assessment of tissue metabolism represents a novel strategy for the evaluation of oncologic disease. Hepatocellular carcinoma (HCC) is a high-prevalence, high-mortality tumor entity often discovered at a late stage. Recent evidence indicates that survival differences depend on metabolic alterations in tumor tissue, with particular focus on glucose metabolism and lactate production. Here, we present an in vivo imaging technique for metabolic tumor phenotyping in rat models of HCC. Endogenous HCC was induced in Wistar rats by oral diethyl-nitrosamine administration. Peak lactate-to-alanine signal ratios (L/A) were assessed with hyperpolarized magnetic resonance spectroscopic imaging (HPMRSI) after [1-13C]pyruvate injection. Cell lines were derived from a subset of primary tumors, re-implanted in nude rats, and assessed in vivo with dynamic hyperpolarized magnetic resonance spectroscopy (HPMRS) after [1-13C]pyruvate injection and kinetic modelling of pyruvate metabolism, taking into account systemic lactate production and recirculation. For ex vivo validation, enzyme activity and metabolite concentrations were spectroscopically quantified in cell and tumor tissue extracts. Mean peak L/A was higher in endogenous HCC compared to non-tumorous tissue. Dynamic HPMRS revealed higher pyruvate-to-lactate conversion rates (kpl) and lactate signal in subcutaneous tumors derived from high L/A tumor cells, consistent with ex vivo measurements of higher lactate dehydrogenase (LDH) levels in these cells. In conclusion, HPMRS and HPMRSI reveal distinct tumor phenotypes corresponding to differences in glycolytic metabolism in HCC tumor tissue.

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