Development of a novel sandwich ELISA to quantify human Tartrate Resistant Acid Phosphatase (TRAP) isoforms 5a and 5b protein in one and the same sample

2016 ◽  
Author(s):  
Christina Patlaka ◽  
Laia Mira-Pascual ◽  
Staffan Paulie ◽  
Pernilla Lang ◽  
Goran Andersson
Keyword(s):  
Acid Phosphatase ◽  
Sandwich Elisa ◽  
Tartrate Resistant Acid Phosphatase

scholarly journals A Novel Sandwich ELISA for Tartrate-Resistant Acid Phosphatase 5a and 5b Protein Reveals that Both Isoforms are Secreted by Differentiating Osteoclasts and Correlate to the Type I Collagen Degradation Marker CTX-I In Vivo and In Vitro

2019 ◽  
Vol 106 (2) ◽  
pp. 194-207 ◽  
Author(s):  
Laia Mira-Pascual ◽  
Christina Patlaka ◽  
Suchita Desai ◽  
Staffan Paulie ◽  
Tuomas Näreoja ◽  
...  
Keyword(s):  
Acid Phosphatase ◽  
Bone Resorption ◽  
Sandwich Elisa ◽  
Osteoclast Differentiation ◽  
Collagen Degradation ◽  
Considerable Proportion ◽  
Tartrate Resistant Acid Phosphatase ◽  
Osteoclast Number ◽  
Serum Trap ◽  
Trap 5B

Abstract Tartrate-resistant acid phosphatase type 5 (TRAP) exists as two isoforms, 5a and 5b. 5b is a marker of osteoclast number and 5a of chronic inflammation; however, its association with bone resorption is unknown. In this study, a double-TRAP 5a/5b sandwich ELISA measuring 5a and 5b protein in the same sample was developed. TRAP 5a and 5b protein levels were evaluated as osteoclast differentiation/activity markers in serum and in culture, and their correlation to the resorption marker CTX-I was examined. Serum TRAP 5a and 5b concentrations in healthy men were 4.4 ± 0.6 ng/ml and 1.3 ± 0.2 ng/ml, respectively, and they correlated moderately to each other suggesting that their secretion is coupled under healthy conditions. A correlation was also observed between serum TRAP 5a and 5b with CTX-I, suggesting that both TRAP isoforms associate with osteoclast number. During osteoclast differentiation on plastic/bone, predominantly 5b increased in media/lysate from M-CSF/RANKL-stimulated CD14+ PBMCs. However, substantial levels of 5a were detected at later stages suggesting that both isoforms are secreted from differentiating OCs. More TRAP 5b was released on bone indicating a connection to osteoclast resorptive activity, and a peak in TRAP 5b/5a-ratio coincided with rapid CTX-I release. At the end of the culture period of M-CSF + RANKL-stimulated CD14+ PBMCs, there was a correlation between the secretion of TRAP 5a and 5b proteins with CTX-I. The correlation of not only 5b but also 5a with collagen degradation, both in serum and osteoclast cultures indicates that a considerable proportion of the TRAP 5a originates from osteoclasts and may reflect a hitherto undisclosed regulatory mechanism during bone resorption and bone remodeling.

Tartrate-Resistant Acid Phosphatase: A Target for Anti-Osteoporotic Chemotherapeutics

2010 ◽  
Vol 6 (3) ◽  
pp. 118-129 ◽  
Author(s):  
Peter Vella ◽  
Ross P. McGeary ◽  
Lawrence R. Gahan ◽  
Gerhard Schenk
Keyword(s):  
Acid Phosphatase ◽  
Tartrate Resistant Acid Phosphatase

scholarly journals Bone-Derived Serum Enzymes and Bone Density in Perimenopausal Caucasian Women

1993 ◽  
Vol 30 (2) ◽  
pp. 191-194 ◽  
Author(s):  
J D Johnston ◽  
S Koneru ◽  
T Kuwana ◽  
S B Rosalki
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Bone Density ◽  
Femoral Neck ◽  
Serum Levels ◽  
Bone Alkaline Phosphatase ◽  
Serum Enzymes ◽  
Tartrate Resistant Acid Phosphatase ◽  
Vertebral Bone ◽  
Caucasian Women

Serum levels of bone-origin alkaline phosphatase and of tartrate-resistant acid phosphatase were measured in Caucasian women aged 41–69 years who had volunteered for bone densitometry. Bone alkaline phosphatase and tartrate-resistant acid phosphatase were inversely correlated with vertebral bone density and with femoral neck bone density. Bone alkaline phosphatase and acid phosphatase were also significantly correlated, consistent with the concept of ‘coupling’ between osteoblast and osteoclast activity.

Confirmation of the Assignment of the Human Tartrate-Resistant Acid Phosphatase Gene (ACP5) to Chromosome 19

Genomics ◽  
1994 ◽  
Vol 19 (1) ◽  
pp. 180-181 ◽  
Author(s):  
Robin J. Leach ◽  
Bonnie E. Reus ◽  
Jeffrey E. Hundley ◽  
Teresa L. Johnson-Pais ◽  
Jolene J. Windle
Keyword(s):  
Acid Phosphatase ◽  
Tartrate Resistant Acid Phosphatase ◽  
Chromosome 19 ◽  
Acid Phosphatase Gene

Tartrate-resistant acid phosphatase is not an exclusive marker for mouse osteoclasts in cell culture

Bone ◽  
1991 ◽  
Vol 12 (2) ◽  
pp. 81-87 ◽  
Author(s):  
W.E. Modderman ◽  
A.C. Tuinenburg-Bol Raap ◽  
P.J. Nijweide
Keyword(s):  
Cell Culture ◽  
Acid Phosphatase ◽  
Tartrate Resistant Acid Phosphatase
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