Medicarpin, a natural pterocarpan, enhances bone regeneration in cortical bone defect model by activation of notch and Wnt canonical signalling pathway

2015 ◽  
Author(s):  
Manisha Dixit ◽  
Ashutosh Raghuvanshi ◽  
Atul Goel ◽  
Divya Singh
Keyword(s):  
Bone Regeneration ◽  
Cortical Bone ◽  
Bone Defect ◽  
Signalling Pathway ◽  
Defect Model

Characterizing gait induced normal strains in a murine tibia cortical bone defect model

2010 ◽  
Vol 43 (14) ◽  
pp. 2765-2770 ◽  
Author(s):  
Jitendra Prasad ◽  
Brett P. Wiater ◽  
Sean E. Nork ◽  
Steven D. Bain ◽  
Ted S. Gross
Keyword(s):  
Cortical Bone ◽  
Bone Defect ◽  
Defect Model

scholarly journals Periosteum progenitors could stimulate bone regeneration in aged murine bone defect model

2020 ◽  
Vol 24 (20) ◽  
pp. 12199-12210
Author(s):  
Han Xiao ◽  
Linfeng Wang ◽  
Tao Zhang ◽  
Can Chen ◽  
Huabin Chen ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Defect ◽  
Defect Model

scholarly journals The Development of Gelatin/Hyaluronate Copolymer Mixed with Calcium Sulfate, Hydroxyapatite, and Stromal-Cell-Derived Factor-1 for Bone Regeneration Enhancement

Polymers ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 1454 ◽  
Author(s):  
Yun-Liang Chang ◽  
Chia-Ying Hsieh ◽  
Chao-Yuan Yeh ◽  
Feng-Huei Lin
Keyword(s):  
Bone Regeneration ◽  
Bone Defect ◽  
Calcium Sulfate ◽  
Stromal Cell ◽  
Femoral Condyle ◽  
Bone Defects ◽  
Blood Analysis ◽  
Control Group ◽  
Defect Model ◽  
Stromal Cell Derived Factor

In clinical practice, bone defects still remain a challenge. In recent years, apart from the osteoconductivity that most bone void fillers already provide, osteoinductivity has also been emphasized to promote bone healing. Stromal-cell-derived factor-1 (SDF-1) has been shown to have the ability to recruit mesenchymal stem cells (MSCs), which play an important role in the bone regeneration process. In this study, we developed a gelatin–hyaluronate (Gel-HA) copolymer mixed with calcium sulfate (CS), hydroxyapatite (HAP), and SDF-1 in order to enhance bone regeneration in a bone defect model. The composites were tested in vitro for biocompatibility and their ability to recruit MSCs after material characterization. For the in vivo test, a rat femoral condyle bone defect model was used. Micro computed tomography (Micro-CT), two-photon excitation microscopy, and histology analysis were performed to assess bone regeneration. As expected, enhanced bone regeneration was well observed in the group filled with Gel-HA/CS/HAP/SDF-1 composites compared with the control group in our animal model. Furthermore, detailed blood analysis of rats showed no obvious systemic toxicity or side effects after material implantation. In conclusion, the Gel-HA/CS/HAP/SDF-1 composite may be a safe and applicable material to enhance bone regeneration in bone defects.

A new standardized critical size bone defect model in the pig forehead for comparative testing of bone regeneration materials

2019 ◽  
Vol 24 (5) ◽  
pp. 1651-1661
Author(s):  
Tobias Moest ◽  
Karl Andreas Schlegel ◽  
Marco Kesting ◽  
Matthias Fenner ◽  
Rainer Lutz ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Defect ◽  
Critical Size ◽  
Defect Model ◽  
Comparative Testing

scholarly journals Predifferentiated Gingival Stem Cell-Induced Bone Regeneration in Rat Alveolar Bone Defect Model

2020 ◽  
Author(s):  
Umadevi Kandalam ◽  
Toshihisa Kawai ◽  
Geeta Ravindran ◽  
Ross Brockman ◽  
Jorge Romero ◽  
...  
Keyword(s):  
Stem Cell ◽  
Bone Regeneration ◽  
Bone Defect ◽  
Alveolar Bone ◽  
Defect Model ◽  
Alveolar Bone Defect

scholarly journals Fabrication of Stromal Cell-Derived Factor-1 Contained in Gelatin/Hyaluronate Copo006Cymer Mixed with Hydroxyapatite for Use in Traumatic Bone Defects

Micromachines ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 822
Author(s):  
Yun-Liang Chang ◽  
Chia-Ying Hsieh ◽  
Chao-Yuan Yeh ◽  
Chih-Hao Chang ◽  
Feng-Huei Lin
Keyword(s):  
Bone Regeneration ◽  
Bone Defect ◽  
Stromal Cell ◽  
Second Harmonic ◽  
Bone Defects ◽  
In Vivo Studies ◽  
Control Group ◽  
Defect Model ◽  
Stromal Cell Derived Factor ◽  
Bone Void

Bone defects of orthopedic trauma remain a challenge in clinical practice. Regarding bone void fillers, besides the well-known osteoconductivity of most bone substitutes, osteoinductivity has also been gaining attention in recent years. It is known that stromal cell-derived factor-1 (SDF-1) can recruit mesenchymal stem cells (MSCs) in certain circumstances, which may also play an important role in bone regeneration. In this study, we fabricated a gelatin/hyaluronate (Gel/HA) copolymer mixed with hydroxyapatite (HAP) and SDF-1 to try and enhance bone regeneration in a bone defect model. After material characterization, these Gel/HA–HAP and Gel/HA–HAP–SDF-1 composites were tested for their biocompatibility and ability to recruit MSCs in vitro. A femoral condyle bone defect model of rats was used for in vivo studies. For the assessment of bone healing, micro-CT analysis, second harmonic generation (SHG) imaging, and histology studies were performed. As a result, the Gel/HA–HAP composites showed no systemic toxicity to rats. Gel/HA–HAP composite groups both showed better bone generation compared with the control group in an animal study, and the composite with the SDF-1 group even showed a trend of faster bone growth compared with the composite without SDF-1 group. In conclusion, in the management of traumatic bone defects, Gel/HA–HAP–SDF-1 composites can be a feasible material for use as bone void fillers.

scholarly journals Biomechanical assessment of freeze-dried allograft cortical bone plate graft in canine bone defect model

2009 ◽  
Vol 54 (No. 4) ◽  
pp. 183-190 ◽  
Author(s):  
S.Y. Heo ◽  
H.B. Lee ◽  
K.C. Lee ◽  
M.S. Kim ◽  
C.S. Na ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Cortical Bone ◽  
Bone Defect ◽  
Bone Screws ◽  
Bone Plate ◽  
Bending Test ◽  
Application Site ◽  
Defect Model ◽  
Freeze Dried ◽  
Significant Difference

Freeze-dried cortical bone can be used as a biological plate, either alone or in combination with other internal fixation devices, to stabilize fractures. In addition to it conferring mechanical stability, freeze-dried cortical bone may enhance fracture-healing and increase the bone stock. This study examined the effect of a freeze-dried allograft cortical bone plate (FACBP) on the biomechanical properties of an implant site in a canine bone defect model. Twelve adult mongrel dogs (around 4.8 kg) were used. A segmental critical-size defect (5 mm in length) at ulna diaphysis was created using an oscillating saw. The experimental animals were divided into two groups: eight dogs treated with an absorbable bone plate (FACBP) fixed by metal bone screws (Group A) and four dogs treated with a commercial stainless steel bone plate and metal bone screws (Group B). Bone healing was assessed by radiography, Dual-energy x-ray absorptiometry and a three-point bending test. The FACBP incorporated in the host bone produced complete remodeling of the cortical bone. There was no significant difference in the bone mineral density and biomechanical tests between the FACBP application site and normal ulna or a stainless steel bone plate of the ulna. These results suggest that FACBP facilitates recovery from a bone fracture by assisting in the induction of new bone formation in a defected fracture.

scholarly journals Formononetin, a methoxy isoflavone, enhances bone regeneration in a mouse model of cortical bone defect

2017 ◽  
Vol 117 (11) ◽  
pp. 1511-1522 ◽  
Author(s):  
Krishna Bhan Singh ◽  
Manisha Dixit ◽  
Kapil Dev ◽  
Rakesh Maurya ◽  
Divya Singh
Keyword(s):  
Bone Regeneration ◽  
Cortical Bone ◽  
Bone Defect ◽  
Bone Healing ◽  
Drill Hole ◽  
Repair Process ◽  
Fracture Repair ◽  
Injury Site ◽  
Healing Effect ◽  
Ct Analysis

AbstractThe bone regeneration and healing effect of formononetin was evaluated in a cortical bone defect model that predominantly heals by intramembranous ossification. For this study, female Balb/c mice were ovariectomised (OVx) and a drill-hole injury was generated in the midfemoral bones of all animals. Treatment with formononetin commenced the day after and continued for 21 d. Parathyroid hormone (PTH1–34) was used as a reference standard. Animals were killed at days 10 and 21. Femur bones were collected at the injury site for histomorphometry studies using microcomputed tomography (μCT) and confocal microscopy. RNA and protein were harvested from the region surrounding the drill-hole injury. For immunohistochemistry, 5 µm sections of decalcified femur bone adjoining the drill-hole site were cut.μCT analysis showed that formononetin promoted bone healing at days 10 and 21 and the healing effect observed was significantly better than in Ovx mice and equal to PTH treatment in many aspects. Formononetin also significantly enhanced bone regeneration as assessed by calcein-labelling studies. In addition, formononetin enhanced the expression of osteogenic markers at the injury site in a manner similar to PTH. Formononetin treatment also led to predominant runt-related transcription factor 2 and osteocalcin localisation at the injury site. These results support the potential of formononetin to be a bone-healing agent and are suggestive of its promising role in the fracture-repair process.

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