Effect of C-reactive protein on TRAP-positive multinucleated cell formation in RANKL-induced RAW264.7 cell culture

2014 ◽  
Author(s):  
Kyoung Hee Choi ◽  
You Cheol Hwang ◽  
In-Kyung Jeong ◽  
Kyu Jeung Ahn ◽  
Ho-Yeon Chung
Keyword(s):  
Cell Culture ◽  
Cell Formation ◽  
Multinucleated Cell ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Raw264.7 Cell

scholarly journals Study of the interaction of the C-reactive protein monomer with the U937 monocyte

2010 ◽  
Vol 15 (3) ◽  
Author(s):  
Jing Zhao ◽  
Xin-He Shi
Keyword(s):  
Foam Cell ◽  
Biological Effects ◽  
Cell Formation ◽  
Density Lipoprotein ◽  
Surface Proteins ◽  
Foam Cell Formation ◽  
C Reactive Protein ◽  
Monocytic Cell ◽  
Monocytic Cell Line ◽  
Reactive Protein

AbstractC-reactive protein (CRP) has two structurally distinct isoforms, the CRP pentamer and the CRP monomer. A role for the CRP monomer in atherosclerosis is emerging, but the underlying mechanisms are only beginning to be understood. Monocytes are an important contributor to atherosclerosis, and foam cell formation is the hallmark of atherogenesis. However, whether the CRP monomer can directly interact with the monocytes and modulate their responses remains unknown. Furthermore, although FcγRIII (CD16) has been identified as the receptor for the CRP monomer on neutrophils, its role in mediating the CRP monomer’s biological effects in other cell types has been questioned. In this study, we investigated the interaction of the CRP monomer with the monocytes using the U937 monocytic cell line. The CRP monomer specifically binds to U937 cells. This binding is unique in that it is independent of FcγRs and insensitive to protease digestion of the cell surface proteins. Further assays revealed that the CRP monomer directly incorporates into the plasma membrane. Interestingly, the presence of the CRP monomer efficiently retards oxidized low-density lipoprotein-induced foam cell formation of PMA-differentiated U937 macrophages and peripheral blood monocytic cell-derived macrophages. These findings provide additional evidence for the notion that the CRP monomer is an active CRP isoform that plays a role in atherogenesis via the direct modulation of the behavior of the monocytes.

Interrelatedness between C-reactive protein and oxidized low-density lipoprotein

2015 ◽  
Vol 53 (1) ◽  
Author(s):  
Milan M. Obradovic ◽  
Andreja Trpkovic ◽  
Vladan Bajic ◽  
Sanja Soskic ◽  
Aleksandra Jovanovic ◽  
...  
Keyword(s):  
Foam Cell ◽  
Oxidized Ldl ◽  
Low Density Lipoprotein ◽  
Cell Formation ◽  
Density Lipoprotein ◽  
High Sensitivity ◽  
Foam Cell Formation ◽  
Low Density ◽  
C Reactive Protein ◽  
Reactive Protein

AbstractC-reactive protein (CRP) is a marker of inflammation. Atherosclerosis is now recognized as inflammatory disease, and it seems that CRP directly contributes to atherogenesis. Oxidation of low-density lipoprotein (LDL) molecule increases the uptake of lipid products by macrophages leading to cholesterol accumulation and subsequent foam cell formation. The elevated levels of high sensitivity CRP (hsCRP) and oxidized LDL (OxLDL) in the blood were found to be associated with cardiovascular diseases (CVD). In this review, we highlighted the evidence that CRP and OxLDL are involved in interrelated (patho) physiological pathways. The findings on association between hsCRP and OxLDL in the clinical setting will be also summarized.

908: Preoperative C-Reactive Protein Elevation as a Predictor of Localized Renal Cell Carcinoma

2007 ◽  
Vol 177 (4S) ◽  
pp. 301-301
Author(s):  
Yasumasa Iimura ◽  
Kazutaka Saito ◽  
Minato Yokoyama ◽  
Hitoshi Masuda ◽  
Tsuyoshi Kobayashi ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Renal Cell ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Localized Renal Cell Carcinoma
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