DNA-damage, survival, differentiation, and matrix mineralization in vitro of a murine multipotent mesenchymal precursor cell line

2013 ◽  
Author(s):  
Patrick Lau ◽  
Yueyuan Hu ◽  
Christine E Hellweg ◽  
Christa Baumstark-Khan ◽  
Gunther Reitz
Keyword(s):  
Dna Damage ◽  
Cell Line ◽  
Precursor Cell ◽  
Matrix Mineralization ◽  
Precursor Cell Line ◽  
Mesenchymal Precursor Cell ◽  
Mesenchymal Precursor

scholarly journals BMPR1A is necessary for chondrogenesis and osteogenesis, whereas BMPR1B prevents hypertrophic differentiation

2020 ◽  
Vol 133 (16) ◽  
pp. jcs246934 ◽  
Author(s):  
Tanja Mang ◽  
Kerstin Kleinschmidt-Doerr ◽  
Frank Ploeger ◽  
Andreas Schoenemann ◽  
Sven Lindemann ◽  
...  
Keyword(s):  
Cell Line ◽  
Precursor Cell ◽  
Osteosarcoma Cell ◽  
Wild Type ◽  
Human Osteosarcoma Cell Line ◽  
Human Osteosarcoma ◽  
Precursor Cell Line ◽  
Human Osteosarcoma Cell ◽  
Mesenchymal Precursor Cell ◽  
Mesenchymal Precursor

ABSTRACTBMP2 stimulates bone formation and signals preferably through BMP receptor (BMPR) 1A, whereas GDF5 is a cartilage inducer and signals preferably through BMPR1B. Consequently, BMPR1A and BMPR1B are believed to be involved in bone and cartilage formation, respectively. However, their function is not yet fully clarified. In this study, GDF5 mutants with a decreased affinity for BMPR1A were generated. These mutants, and wild-type GDF5 and BMP2, were tested for their ability to induce dimerization of BMPR1A or BMPR1B with BMPR2, and for their chondrogenic, hypertrophic and osteogenic properties in chondrocytes, in the multipotent mesenchymal precursor cell line C3H10T1/2 and the human osteosarcoma cell line Saos-2. Mutants with the lowest potency for inducing BMPR1A–BMPR2 dimerization exhibited minimal chondrogenic and osteogenic activities, indicating that BMPR1A is necessary for chondrogenic and osteogenic differentiation. BMP2, GDF5 and the GDF5 R399E mutant stimulated expression of chondrogenic and hypertrophy markers in C3H10T1/2 cells and chondrocytes. However, GDF5 R399E, which induces the dimerization of BMPR1B and BMPR2 more potently than GDF5 or BMP2, displayed reduced hypertrophic activity. Therefore, we postulate that stronger BMPR1B signaling, compared to BMPR1A signaling, prevents chondrocyte hypertrophy and acts as a cartilage stabilizer during joint morphogenesis.This article has an associated First Person interview with the first author of the paper.

Inducible expression of FGF2 by a rat oligodendrocyte precursor cell line promotes CNS myelination in vitro

2003 ◽  
Vol 184 (2) ◽  
pp. 912-922 ◽  
Author(s):  
Laurent Magy ◽  
Caroline Mertens ◽  
Virginia Avellana-Adalid ◽  
Mahamane Keita ◽  
François Lachapelle ◽  
...  
Keyword(s):  
Cell Line ◽  
Inducible Expression ◽  
Precursor Cell ◽  
Oligodendrocyte Precursor Cell ◽  
Precursor Cell Line ◽  
Oligodendrocyte Precursor ◽  
Cns Myelination

An in vitro osteoblast culture model using estrogen responsive Ks483 mouse osteoblast precursor cell line

Bone ◽  
2010 ◽  
Vol 47 ◽  
pp. S120
Author(s):  
K.M. Fagerlund ◽  
J.P. Rissanen ◽  
T. Suutari ◽  
A. Chan ◽  
J.M. Halleen
Keyword(s):  
Cell Line ◽  
Precursor Cell ◽  
Osteoblast Culture ◽  
Precursor Cell Line ◽  
Culture Model ◽  
Mouse Osteoblast ◽  
Osteoblast Precursor Cell

Functional V region formation during in vitro culture of a murine immature B precursor cell line

Nature ◽  
1983 ◽  
Vol 303 (5920) ◽  
pp. 812-815 ◽  
Author(s):  
Haruo Sugiyama ◽  
Shizuo Akira ◽  
Hitoshi Kikutani ◽  
Susumu Kishimoto ◽  
Yuichi Yamamura ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Cell Line ◽  
Precursor Cell ◽  
Precursor Cell Line ◽  
V Region

Characterization of a mouse tet-on glia precursor cell line in vitro and in vivo using the electrophysiological measurement

2002 ◽  
Vol 96 (3-4) ◽  
pp. 329-338 ◽  
Author(s):  
Christian Elmshäuser ◽  
Juliane Bechtel ◽  
Iris Motta ◽  
Carola Schipke ◽  
Helmut Kettenmann ◽  
...  
Keyword(s):  
Cell Line ◽  
Precursor Cell ◽  
Precursor Cell Line ◽  
Electrophysiological Measurement
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