Regulation of cell growth, c-myc mRNA, and 1,25-(OH)2 vitamin D3 receptor in C3H/10T1/2 mouse embryo fibroblasts by calcipotriol and 1,25-(OH)2 vitamin D3

1992 ◽  
Vol 126 (1) ◽  
pp. 75-79 ◽  
Author(s):  
Torleif Trydal ◽  
Johan R. Lillehaug ◽  
Lage Aksnes ◽  
Dagfinn Aarskog
Keyword(s):  
Cell Growth ◽  
Cell Line ◽  
Cell Lines ◽  
Vitamin D3 ◽  
Mouse Embryo ◽  
Transformed Cell ◽  
Mouse Embryo Fibroblasts ◽  
Transformed Cell Line ◽  
Embryo Fibroblasts ◽  
Transformed Cell Lines

Calcipotriol is a synthetic 1,25-(OH)2D3 analogue with high affinity for the 1,25-(OH)2D3 receptor, but with a lower affinity than 1,25-(OH)2D3 for vitamin D binding protein in serum. The inhibitory action of calcipotriol and 1,25-(OH)2D3 on proliferation of C3H/10T1/2 mouse embryo fibroblasts was examined in the non-transformed cell line CI 8 and in the two transformed, tumorigenic cell lines Cl 16 and TPA 482. Upon exposure to 10 nmol/l calcipotriol or 1,25-(OH)2D3, the proliferation of Cl 8 cell line was almost completely suppressed, whereas both hormones had no effect on the cell lines Cl 16 and TPA 482. Calcipotriol was at least as effective as 1,25-(OH)2D3 in inducing up-regulation of the 1,25-(OH)2D3 receptor. Displacement studies showed no difference between calcipotriol and 1,25-(OH)2D3 in the affinity for the receptor present in Cl 8 or Cl 16 cell extracts. Furthermore, the inhibition of cell growth in Cl 8 cells by calcipotriol was not accompanied by any consistent change in the steady-state expression of c-myc mRNA. In conclusion, calcipotriol had potent growth inhibitory effect on the non-transformed cell line similar to 1,25-(OH)2D3. In the transformed cell lines, calcipotriol did not inhibit proliferation despite potent up-regulation of the 1,25-(OH)2D3 receptor.

scholarly journals Porcine pancreatic ductal epithelial cells transformed with KRASG12D and SV40T are tumorigenic

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Katie L. Bailey ◽  
Sara B. Cartwright ◽  
Neesha S. Patel ◽  
Neeley Remmers ◽  
Audrey J. Lazenby ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Animal Model ◽  
Epithelial Cells ◽  
Cell Line ◽  
Cell Lines ◽  
Large Animal Model ◽  
Large Animal ◽  
Transformed Cell ◽  
Transformed Cell Line ◽  
Transformed Cell Lines

AbstractWe describe our initial studies in the development of an orthotopic, genetically defined, large animal model of pancreatic cancer. Primary pancreatic epithelial cells were isolated from pancreatic duct of domestic pigs. A transformed cell line was generated from these primary cells with oncogenic KRAS and SV40T. The transformed cell lines outperformed the primary and SV40T immortalized cells in terms of proliferation, population doubling time, soft agar growth, transwell migration and invasion. The transformed cell line grew tumors when injected subcutaneously in nude mice, forming glandular structures and staining for epithelial markers. Future work will include implantation studies of these tumorigenic porcine pancreatic cell lines into the pancreas of allogeneic and autologous pigs. The resultant large animal model of pancreatic cancer could be utilized for preclinical research on diagnostic, interventional, and therapeutic technologies.

scholarly journals Porcine pancreatic ductal epithelial cells transformed with KRAS G12D and SV40T are tumorigenic

2021 ◽  
Author(s):  
Katie L. Bailey ◽  
Sara B. Cartwright ◽  
Neesha S. Patel ◽  
Neeley Remmers ◽  
Audrey J. Lazenby ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Animal Model ◽  
Epithelial Cells ◽  
Cell Line ◽  
Cell Lines ◽  
Large Animal Model ◽  
Large Animal ◽  
Transformed Cell ◽  
Transformed Cell Line ◽  
Transformed Cell Lines

Abstract We describe our initial studies in the development of an orthotopic, genetically-defined, large animal model of pancreatic cancer. Primary pancreatic epithelial cells were isolated from pancreatic duct of domestic pigs. A transformed cell line was generated from these primary cells with oncogenic KRAS and SV40T. The transformed cell lines outperformed the primary and SV40T immortalized cells in terms of proliferation, population doubling time, soft agar growth, transwell migration and invasion. The transformed cell line grew tumors when injected subcutaneously in nude mice, forming glandular structures and staining for epithelial markers. Future work will include implantation studies of these tumorigenic porcine pancreatic cell lines into the pancreas of allogeneic and autologous pigs. The resultant large animal model of pancreatic cancer could be utilized for preclinical research on diagnostic, interventional, and therapeutic technologies.

scholarly journals Porcine pancreatic ductal epithelial cells transformed with KRASG12D and SV40T are tumorigenic

2021 ◽  
Author(s):  
Katie Bailey ◽  
Sara B. Cartwright ◽  
Neesha S. Patel ◽  
Neeley Remmers ◽  
Audrey J. Lazenby ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Animal Model ◽  
Epithelial Cells ◽  
Cell Line ◽  
Cell Lines ◽  
Large Animal Model ◽  
Large Animal ◽  
Transformed Cell ◽  
Transformed Cell Line ◽  
Transformed Cell Lines

AbstractWe describe our initial studies in the development of an orthotopic, genetically-defined, large animal model of pancreatic cancer. Primary pancreatic epithelial cells were isolated from pancreatic duct of domestic pigs. A transformed cell line was generated from these primary cells with oncogenic KRAS and SV40T. The transformed cell lines outperformed the primary and SV40T immortalized cells in terms of proliferation, population doubling time, soft agar growth, transwell migration and invasion. The transformed cell line grew tumors when injected subcutaneously in nude mice, forming glandular structures and staining for epithelial markers. Future work will include implantation studies of these tumorigenic porcine pancreatic cell lines into the pancreas of allogeneic and autologous pigs. The resultant large animal model of pancreatic cancer could be utilized for preclinical research on diagnostic, interventional, and therapeutic technologies.

scholarly journals Effect of tunicamycin on cell growth and morphology of nontransformed and transformed cell lines.

1979 ◽  
Vol 43 (7) ◽  
pp. 1553-1561 ◽  
Author(s):  
Kenji KOHNO ◽  
Akiyoshi HIRAGUN ◽  
Hiromi MITSUI ◽  
Akira TAKATSUKI ◽  
Gakuzo TAMURA
Keyword(s):  
Cell Growth ◽  
Cell Lines ◽  
Transformed Cell ◽  
Transformed Cell Lines

scholarly journals Differential ability of a T-antigen transport-defective mutant of simian virus 40 to transform primary and established rodent cells.

1985 ◽  
Vol 5 (5) ◽  
pp. 1043-1050 ◽  
Author(s):  
R E Lanford ◽  
C Wong ◽  
J S Butel
Keyword(s):  
Cell Lines ◽  
Mouse Embryo ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Wild Type ◽  
Mouse Embryo Fibroblasts ◽  
Established Cell Lines ◽  
Established Cell ◽  
Embryo Fibroblasts

The transforming potential and oncogenicity of a simian virus 40 (SV40) mutant affecting T-antigen (T-ag), SV40(cT)-3, was examined in an effort to dissect T-ag functions in transformation. SV40(cT)-3 has a point mutation at nucleotide 4434 that abolishes the transport of T-ag to the nucleus but does not affect its association with the cell surface. Transfection-transformation assays were performed with primary cells and established cell lines of mouse and rat origin. The efficiency of transformation for established cell lines by SV40(cT)-3 was comparable to that of wild-type SV40, indicating that transformation of established cell lines can occur in the absence of detectable amounts of nuclear T-ag. Transformation of primary mouse embryo fibroblasts by SV40(cT)-3 was markedly influenced by culture conditions; the relative transforming frequency was dramatically reduced in assays involving focus formation in low serum concentrations or anchorage-independent growth. Immunofluorescence tests revealed that the transformed mouse embryo fibroblasts partially transport the mutant cT-ag to the cell nucleus. Transformed cell lines induced by SV40(cT)-3 did not differ in growth properties from wild-type transformants. SV40(cT)-3 was completely defective for the transformation of primary baby rat kidney cells, a primary cell type unable to transport the mutant T-ag to the nucleus. The intracellular localization of cellular protein p53 was found to mimic T-ag distribution in all the transformants analyzed. The mutant virus was weakly oncogenic in vivo: the induction of tumors in newborn hamsters by SV40(cT)-3 was reduced in incidence and delayed in appearance in comparison to wild-type SV40. These observations suggest that cellular transformation is regulated by both nuclear and surface-associated forms of SV40 T-ag.

Effect of Tunicamycin on Cell Growth and Morphology of Nontransformed and Transformed Cell Lines

1979 ◽  
Vol 43 (7) ◽  
pp. 1553-1561
Author(s):  
Kenji Kohno ◽  
Akiyoshi Hiragun ◽  
Hiromi Mitsui ◽  
Akira Takatsuki ◽  
Gakuzo Tamura
Keyword(s):  
Cell Growth ◽  
Cell Lines ◽  
Transformed Cell ◽  
Transformed Cell Lines

scholarly journals Multiple RSV strains infecting HEp-2 and A549 cells reveal cell line-dependent differences in resistance to RSV infection.

2021 ◽  
Author(s):  
Anubama Rajan ◽  
Felipe-Andres Piedra ◽  
Letisha Aideyan ◽  
Trevor McBride ◽  
Matthew J Robertson ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Cell Lines ◽  
A549 Cells ◽  
Viral Gene Expression ◽  
Model Systems ◽  
Viral Gene ◽  
Transformed Cell ◽  
Syncytial Virus ◽  
Transformed Cell Lines

Respiratory syncytial virus (RSV) is a leading cause of pediatric acute respiratory infection worldwide. There are currently no approved vaccines or antivirals to combat RSV disease. A few transformed cell lines and two historic strains have been extensively used to study RSV. Here we report a thorough molecular and cell biological characterization of HEp-2 and A549 cells infected with four strains of RSV representing both major subgroups as well as historic and more contemporaneous genotypes -- [RSV/A/Tracy (GA1), RSV/A/Ontario (ON), RSV/B/18537 (GB1), RSV/B/Buenos Aires (BA)] -- via measurements of viral replication kinetics and viral gene expression, immunofluorescence-based imaging of gross cellular morphology and cell-associated RSV, and measurements of host response including transcriptional changes and levels of secreted cytokines and growth factors. Our findings strongly suggest 1) the existence of a conserved difference in gene expression between RSV subgroups A and B; 2) the A549 cell line is a more stringent and natural host of replicating RSV than the HEp-2 cell line; and 3) consistent with previous studies, determining the full effects of viral genetic variation in RSV pathogenesis requires model systems as tractable as transformed cell lines but better representative of the human host.

Effects of mevinolin and mevalonate on cell growth in several transformed cell lines

1986 ◽  
Vol 127 (2) ◽  
pp. 216-222 ◽  
Author(s):  
Kathy P. Fairbanks ◽  
Veronique D. Barbu ◽  
Larry D. Witte ◽  
I. Bernard Weinstein ◽  
DeWitt S. Goodman
Keyword(s):  
Cell Growth ◽  
Cell Lines ◽  
Transformed Cell ◽  
Transformed Cell Lines

scholarly journals Cell-surface mucosubstances from trypsin disaggregation of normal and virus-transformed lines of baby-hamster kidney cells (Short Communication)

1973 ◽  
Vol 134 (4) ◽  
pp. 1123-1126 ◽  
Author(s):  
S. Megan Minnikin ◽  
Adrian Allen
Keyword(s):  
Cell Surface ◽  
Cell Line ◽  
Cell Lines ◽  
Short Communication ◽  
Polyoma Virus ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Transformed Cell ◽  
Transformed Cell Line ◽  
Baby Hamster Kidney Cells

Cell disaggregation by trypsin solubilizes significantly less mucosubstance from the surface of polyoma-virus-transformed baby-hamster kidney cells than from the same non-transformed cell line. The mucosubstance, which consists of both acid mucopolysaccharides and mucoproteins, also differs qualitatively in the two cell lines.

scholarly journals Introduction and recovery of a selectable bacterial gene from the genome of mammalian cells

1982 ◽  
Vol 2 (8) ◽  
pp. 966-976
Author(s):  
M L Breitman ◽  
L C Tsui ◽  
M Buchwald ◽  
L Siminovitch
Keyword(s):  
Molecular Weight ◽  
Cell Line ◽  
Cell Lines ◽  
Mammalian Cells ◽  
Dna Molecules ◽  
High Molecular Weight Dna ◽  
Bacterial Gene ◽  
E Coli ◽  
Transformed Cell ◽  
Transformed Cell Lines

The simian virus 40 (SV40)-pBR322 recombinant, pSV2, carrying the origin of SV40 replication and the gpt gene of Escherichia coli, has been stably introduced into Chinese hamster ovary hprt- cells. All gpt-transformed cell lines were found to contain one or more insertions of pSV2 sequences exclusively associated with high-molecular-weight DNA. Additional analyses showed that at least one integrated copy in each cell line retained an intact gpt gene and flanking SV40 sequences required for expression of xanthine-guanine phosphoribosyltransferase. Most cell lines contained pSV2 sequences which had integrated with partial sequence duplication. Upon fusion with COS-1 cells, a simian cell line permissive for autonomous pSV2 replication, most gpt-transformed cell lines produced low-molecular-weight DNA molecules related to pSV2. The majority of these replicating DNAs were indistinguishable from the original transfecting plasmid in both size and restriction enzyme cleavage pattern. In addition, the recovered DNA molecules were able to confer ampicillin resistance to E. coli and to transform mouse L cells and Gpt- E. coli to a Gpt+ phenotype. These studies indicate that all of the genetic information carried by this SV40-plasmid recombinant can be introduced into and retrieved from the genome of mammalian cells.

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