Different binding of stimulatory-type and blocking-type TSH receptor antibody with guinea-pig testis membrane

1991 ◽  
Vol 125 (5) ◽  
pp. 563-569 ◽  
Author(s):  
T. Inui ◽  
Y. Ochi ◽  
T. Hachiya ◽  
W. Chen ◽  
Y. Nakajima ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Inhibitory Effect ◽  
Primary Hypothyroidism ◽  
Significant Inhibition ◽  
Tsh Receptor ◽  
Α Subunit ◽  
Blocking Antibody ◽  
Thyroid Stimulating Antibody ◽  
Receptor Antibodies ◽  
Tsh Receptor Antibodies

Abstract. A receptor assay using [125I]bTSH-binding to guinea-pig testis membrane was developed. Unlabelled hCG and FSH inhibited [125I]bTSH binding. In patients with Graves' disease and in untreated hyperthyroid patients, almost all long-acting thyroid stimulators and thyroid-stimulating antibodies, respectively did not inhibit [125I]bTSH binding, which on the other hand was inhibited by thyroid stimulation blocking antibodies in patients with primary hypothyroidism. When the inhibitory effect on the binding of [125I]hCG and 125I-synthetic α-subunit peptide (α26-46) of hCG to testis membrane was examined, bTSH resulted in a significant inhibition. However, all three kinds of TSH receptor antibodies had no inhibitory effect. This study demonstrated 1. interaction of α-subunit of TSH and hCG with the testicular receptor; 2. binding of thyroid stimulation-blocking antibody and lack of binding of thyroid-stimulating antibody to the testicular TSH receptor in spite of binding of these TSH receptor antibodies to the thyroidal TSH receptor, and 3. lack of binding of thyroid-stimulating antibody and thyroid stimulation-blocking antibody to the testicular gonadotropin receptor.

Anti-TSH antibodies in Graves' disease and their failure to interact with TSH receptor antibodies

1989 ◽  
Vol 120 (6) ◽  
pp. 773-777 ◽  
Author(s):  
Y. Ochi ◽  
T. Nagamune ◽  
Y. Nakajima ◽  
M. Ishida ◽  
Y. Kajita ◽  
...  
Keyword(s):  
Primary Hypothyroidism ◽  
Tsh Receptor ◽  
Graves Disease ◽  
Antibody Technique ◽  
Α Subunit ◽  
Occasional Occurrence ◽  
Assay Methods ◽  
Receptor Antibodies ◽  
Hyperthyroid Patients ◽  
Tsh Receptor Antibodies

Abstract. The occasional occurrence in sera of patients with Graves' disease of negative values in the assay for TSH receptor antibodies led to the discovery of endogenous antibodies to TSH. We examined the sera of approximately 2500 patients with Graves' disease. Eight positive sera were found. The IgG in all 8 sera showed higher binding with both bTSH and porcine TSH (pTSH) than with human TSH (hTSH). This means that autoantibodies to TSH in sera from patients with Graves' disease are rare and often directed towards heterologous bovine and porcine TSH. When hTSH levels were determined in sera of hyperthyroid patients with positive antibodies to hTSH, discrepancies in serum hTSH levels were observed when using different assay methods, i.e. hTSH levels were higher with the double-antibody technique, and lower with immunoradiometric assays. Antibodies in these sera showed higher binding to pTSH-α subunit than to -β subunit. The binding of the two pTSH subunits with antibodies could be displaced by intact bTSH. Neither stimulation in Graves' disease nor blocking in primary hypothyroidism of TSH receptor antibodies interfered with the binding of the anti-TSH antibodies to 125I-labelled pTSH, pTSH-α, and pTSH-β. Consequently, using this type of autoantibodies to TSH we were unable to obtain evidence that the TSH receptor antibodies of patients with Graves' disease was an anti-idiotype antibody against anti-TSH antibodies.

The TSH receptor: Structure and interaction with autoantibodies in thyroid disease

1987 ◽  
Vol 116 (1_Suppl) ◽  
pp. S157-S165 ◽  
Author(s):  
J. Furmaniak ◽  
Y. Nakajima ◽  
F. A. Hashim ◽  
F. M. Creagh ◽  
E. Davies Jones ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Thyroid Tissue ◽  
Tsh Receptor ◽  
Sds Page ◽  
Affinity Labelling ◽  
Stokes Radius ◽  
A Subunit ◽  
Receptor Antibodies ◽  
Derivatives Of ◽  
Tsh Receptor Antibodies

Abstract. Studies of the TSH receptor using affinity labelling with photoactive derivatives of TSH and analysis by SDS-PAGE have shown that the receptor contains 2 subunits (A and B), linked by a disulphide bridge. Similar results are obtained with TSH receptors from human, porcine and guinea pig thyroid tissue and from guinea pig fat. Analysis of affinity labelled receptors under non-denaturing conditions suggest that subunits additional to the A and B subunits are not present. Hydrodynamic measurements indicate that the receptor A subunit has an approximately spherical structure (Stokes' radius 70Å) and when this interacts with TSH (an elongated structure with Stokes' radius 56Å) a very elongated complex (Stokes' radius 104Å) is formed. Isoelectric focusing studies of the TSH receptor A subunit, TSH and TSH receptor antibodies indicate that charge-charge interactions are of considerable importance in the binding of hormone and antibody to the receptor.

scholarly journals Hyperthyroid Graves' Disease and Primary Hypothyroidism Caused by TSH Receptor Antibodies in Monozygotic Twins: Case Reports.

1998 ◽  
Vol 45 (1) ◽  
pp. 117-121 ◽  
Author(s):  
JUN-ICHI TANI ◽  
KATSUMI YOSHIDA ◽  
HIROSHI FUKAZAWA ◽  
YOSHINORI KISO ◽  
NORIYO SAYAMA ◽  
...  
Keyword(s):  
Case Reports ◽  
Monozygotic Twins ◽  
Primary Hypothyroidism ◽  
Tsh Receptor ◽  
Graves Disease ◽  
Receptor Antibodies ◽  
Tsh Receptor Antibodies

TSH Receptor Antibodies

Thyroid ◽  
2007 ◽  
Vol 17 (10) ◽  
pp. 923-938 ◽  
Author(s):  
Bernard Rees Smith ◽  
Jane Sanders ◽  
Jadwiga Furmaniak
Keyword(s):  
Tsh Receptor ◽  
Receptor Antibodies ◽  
Tsh Receptor Antibodies

Inhibition of thyrotropin-stimulated adenylate cyclase activation and growth of rat thyroid cells, FRTL-5, by immunoglobulin G from patients with primary myxedema: comparison with activities of thyrotropin-binding inhibitor immunoglobulins

1989 ◽  
Vol 120 (1) ◽  
pp. 99-106 ◽  
Author(s):  
B. Y. Cho ◽  
Y. K. Shong ◽  
H. K. Lee ◽  
C.-S. Koh ◽  
H. K. Min
Keyword(s):  
Hashimoto’S Thyroiditis ◽  
Thymidine Incorporation ◽  
Tsh Receptor ◽  
Hashimoto's Thyroiditis ◽  
Thyroid Cells ◽  
Rat Thyroid ◽  
Receptor Antibodies ◽  
Tsh Receptor Antibodies ◽  
Growth Inhibiting ◽  
Blocking Type

Abstract. We studied the blocking type TSH receptor antibodies in 28 patients with primary myxedema and 21 patients with goitrous Hashimoto's thyroiditis by measuring the ability of their IgGs to inhibit TSH binding to its receptor, and to inhibit TSH-stimulated cAMP increase and [3H] thymidine incorporation in a rat thyroid cell line, FRTL-5. The incidences of TSH binding inhibitor immunoglobulin, thyroid stimulation inhibiting immunoglobulin and thyroid growth inhibiting immunoglobulin in patients with primary myxedema were 54.6, 75 and 65.2%, respectively, against 14.3,0 and 17.7%, respectively, in goitrous Hashimoto's thyroiditis. The antibodies inhibited dose-dependently not only TSH stimulated but also Graves' IgG-stimulated cAMP increase and [3H] thymidine incorporation. The TSH binding inhibitor immunoglobulin activities in patients with primary myxedema were significantly correlated with both the thyroid stimulation inhibiting immunoglobulin (r = 0.665; P<0.01) and the thyroid growth inhibiting immunoglobulin (r = 0.618; P<0.01) activity. Thirteen patients whose TSH binding inhibitor immunoglobulin activities were more than 50% had both strong thyroid stimulation inhibiting immunoglobulin (75.1–100%) and thyroid growth inhibiting immunoglobulin (57.4–100%) activities. These data suggest that the vast majority of patients with primary myxedema have potent blocking type TSH receptor antibodies. These might play a role in primary myxedema causing hypothyroidism and thyroid atrophy through inhibiting TSH-stimulated cAMP generation.

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