Effect of lithium chloride on testicular steroidogenesis and gametogenesis in immature male rats

1991 ◽  
Vol 124 (1) ◽  
pp. 76-82 ◽  
Author(s):  
P. K. Ghosh ◽  
N. M. Biswas ◽  
D. Ghosh
Keyword(s):  
Lithium Chloride ◽  
Sperm Count ◽  
Serum Levels ◽  
Hydroxysteroid Dehydrogenase ◽  
Significant Inhibition ◽  
Male Rats ◽  
Hormonal Changes ◽  
Subcutaneous Injections ◽  
Immature Male ◽  
Testicular Steroidogenesis

Abstract. The present study was performed on immature male rats aged 35 days. Subcutaneous injections of lithium chloride at a daily dose of 2.0 mg/kg for 15 days resulted in significant inhibition of spermatogenesis at stage VII of the seminiferous epithelial cycle. Spermatogonia A, preleptotene spermatocytes and step 7 spermatids were decreased in number in comparison to controls. Serum levels of FSH, LH, PRL, and testosterone were decreased. Activities of testicular Δ5-3β-hydroxysteroid dehydrogenase and 17β-hydroxysteroid dehydrogenase were suppressed along with a low caudal epididymal sperm count in comparison with controls. When the treatment was prolonged for 20 and 25 days, it showed an additional significant diminution in accessory sex organ weights and number of midpachytene spermatocytes at stage VII in comparison to control animals of corresponding age. It is concluded that lithium has an adverse effect on testicular function in immature rats by reducing serum levels of FSH, LH, PRL, and testosterone. Furthermore, since hormonal changes and altered spermatogenic activities were evident when the serum concentration of lithium was within the therapeutic range, our data may have some potential clinical implications.

Studies on the effect of prolactin treatment on testicular steroidogenesis and gametogenesis in lithium-treated rats

1991 ◽  
Vol 125 (3) ◽  
pp. 313-318 ◽  
Author(s):  
D. Ghosh ◽  
N. M. Biswas ◽  
P. K. Ghosh
Keyword(s):  
Lithium Chloride ◽  
Lithium Treatment ◽  
Serum Levels ◽  
Hydroxysteroid Dehydrogenase ◽  
Significant Inhibition ◽  
Steroidogenic Enzymes ◽  
Subcutaneous Injections ◽  
Testicular Dysfunction ◽  
Testicular Steroidogenesis ◽  
Chloride Treatment

Abstract. The effect of PRL supplementation in lithium-treated rats on spermatogenesis, testicular Δ5-3β-hydroxysteroid dehydrogenase and 17β-hydroxysteroid dehydrogenase activities, and serum levels of FSH, LH, PRL and testosterone were studied on the 22nd day of the experiment. Subcutaneous injections of lithium chloride at a dose of 2.0 mg·kg−1·day−1 for 21 days resulted in a significant inhibition of spermatogenesis at stage VII of the seminiferous epithelial cycle, along with remarkable diminution of serum levels of the above hormones and suppression of the activities of the above two testicular steroidogenic enzymes. Administration of bovine PRL at a dose of 0.25 mg·kg−1·day−1 plus lithium treatment resulted in a remarkable protection of spermatogenic and steroidogenic activities of the testes, along with restoration of serum levels of FSH and testosterone. It is concluded that PRL can markedly protect the testicular dysfunction induced by lithium chloride treatment in rats.

scholarly journals CONCENTRATION OF THE GONADOTROPIC HORMONE IN PREGNANT MARE'S SERUM

1933 ◽  
Vol 58 (5) ◽  
pp. 569-574 ◽  
Author(s):  
Herbert M. Evans ◽  
Edwin L. Gustus ◽  
Miriam E. Simpson
Keyword(s):  
Aluminum Hydroxide ◽  
Physiological Saline ◽  
Seminal Vesicles ◽  
Male Rats ◽  
Subcutaneous Injections ◽  
Gonadotropic Hormone ◽  
Female Mice ◽  
Immature Male ◽  
Active Aluminum

The gonadotropic hormone of the blood of the pregnant mare has been greatly concentrated by adsorption on active aluminum hydroxide followed by elution. The preparations so obtained gave demonstrable gonadotropic effects within 100 hours in 21 day old female mice following three subcutaneous injections of 0.001 mg. in 1 cc. of physiological saline. As is well known, other gonadotropic substances do not cause conspicuous development of the male gonads but injections of comparatively large doses of these preparations into immature male rats caused marked development of the testes, which in 10 days were trebled in weight. An astonishing increase in the weight of the seminal vesicles resulted, for these organs were approximately 75 times heavier than in controls.

scholarly journals Investigation Into Effect Of Crude Mixture Of Abrus Precatorius Seed On Hypothalamopituitary Gonadal Axis And Development Of Antifertility In Male Rats

1970 ◽  
Vol 36 (1) ◽  
pp. 103-109 ◽  
Author(s):  
S Talukder ◽  
MA Hossain ◽  
S Sarker ◽  
MAH Khan
Keyword(s):  
Negative Impact ◽  
Sperm Count ◽  
Sharp Decrease ◽  
Serum Levels ◽  
Serum Biochemistry ◽  
Male Rats ◽  
Male Rat ◽  
Control Group ◽  
Abrus Precatorius ◽  
Crude Mixture

To evaluate the antifertility effect of crude mixture of A. precatorius seeds at the dose level of 50 mg/kg body weight in adult male rats, after oral administration to male rats for 40 days, the rats were sacrificed and hormonal profiles, serum biochemistry, sperm count and histological changes were recorded. A sharp decrease in the serum levels of testosterone (0.70 ± 0.17 ng/ml), FSH (0.70 ± 0.22 lU/L), and LH (0.87 ± 0.35 IU/L) was detected compared to control (FSH, LH and testosterone levels 0.93 ± 0.15 ng/ml, 0.76 ± 0.28 IU/L, 1.44 ± .011 IU/L, respectively). A significant reduction of epididymal sperm count (2.34 million/mL) was noted in treated rats as compared to control group (7.87 million/mL). Histology of testes showed marked atrophy of the testes, which was characterized by disruption of the seminiferous epithelium and atrophy of the Leydig cells. Crude mixture of A. precatorius seed has a negative impact on male reproductive functions. It might be suggested that crude mixture of A. precatorius seeds might have antifertility property for male rats.   Keywords: Abrus precatorius; antifertility; male rat; testosterone. DOI: http://dx.doi.org/10.3329/bjar.v36i1.9234 BJAR 2011; 36(1): 103-109

Effect of α2u-globulin on serum concentration of gonadotrophins and testicular activity in oestrogen-treated rats

1983 ◽  
Vol 96 (2) ◽  
pp. 321-327 ◽  
Author(s):  
N. M. Biswas ◽  
P. K. Ghosh ◽  
K. K. Ghosh ◽  
O. W. Neuhaus
Keyword(s):  
Serum Concentration ◽  
Adult Male ◽  
Serum Levels ◽  
Hydroxysteroid Dehydrogenase ◽  
Organ Weight ◽  
Male Rats ◽  
Testicular Function ◽  
Accessory Sex Organs ◽  
Testosterone Synthesis

Adult male rats were given injections of oestradiol-17β (50 μg/100 g body wt per day) for 7 days. When they were killed 14 days after the last injection, serum levels of gonadotrophins and testosterone and weights of accessory sex organs were less, testicular 17β-hydroxysteroid dehydrogenase 17β-HSD activity was suppressed and spermatogenesis was inhibited. Administration of α2u-globulin (1·5 mg/day) for 14 days to oestrogen-treated rats and for 10 days to control rats resulted in increased concentrations of gonadotrophins and testosterone in the serum. Accessory sex organ weight and spermatogenesis appeared to be normal while 17β-HSD activity increased in oestrogen-treated rats after treatment with α2u-globulin. It was concluded that α2u-globulin has an effect on testicular function in oestrogenized rats by inducing gonadotrophin and testosterone synthesis.

Effect of dihydrotestosterone on serum concentrations of α2u-globulin and on spermatogenesis in melatonin-treated rats

1990 ◽  
Vol 126 (3) ◽  
pp. 431-435 ◽  
Author(s):  
H. Mandal ◽  
P. K. Ghosh ◽  
N. M. Biswas
Keyword(s):  
Body Weight ◽  
Adult Male ◽  
Serum Levels ◽  
Hydroxysteroid Dehydrogenase ◽  
Male Rats ◽  
Testicular Function ◽  
Serum Concentrations ◽  
Melatonin Treatment ◽  
Accessory Sex Organs

ABSTRACT Adult male rats were given s.c. injections of melatonin (400 μg/100 g body weight per day) for 14 days. On day 15, the weights of the testis and accessory sex organs were less, testicular 17β-hydroxysteroid dehydrogenase (17β-HSD) activity was inhibited, spermatogenesis was suppressed and serum levels of gonadotrophins, testosterone and α2u-globulin were decreased compared with control animals injected with vehicle. In a third group of rats given the same dose of melatonin for 14 days, administration of dihydrotestosterone (DHT) at a dose of 25 μg/100 g body weight per day on days 8–14 resulted in serum levels of α2u-globulin, FSH, LH and testosterone and testicular 17β-HSD activity similar to those seen in vehicle-injected control animals. Weights of the testes and accessory sex organs and spermatogenesis were normal after administration of DHT in melatonintreated rats. In another group of rats, the depressive effects of melatonin treatment on plasma gonadotrophins were reversed by the administration of α2u-globulin on days 8–14. It was concluded that treatment with DHT prevents the depressive action of melatonin on testicular function by inducing the synthesis of α2u-globulin. Journal of Endocrinology (1990) 126, 431–435

MECHANISM(S) BY WHICH ADRENALECTOMY AND CORTICOSTERONE INFLUENCE PROLACTIN RELEASE IN THE RAT

1980 ◽  
Vol 87 (1) ◽  
pp. 131-NP ◽  
Author(s):  
F. C. LEUNG ◽  
H. T. CHEN ◽  
S. J. VERKAIK ◽  
R. W. STEGER ◽  
J. J. PELUSO ◽  
...  
Keyword(s):  
Serum Levels ◽  
Thyroid Stimulating Hormone ◽  
Significant Inhibition ◽  
Male Rats ◽  
Prolactin Release ◽  
Medial Basal Hypothalamus ◽  
Hypophysectomized Rats ◽  
Adrenalectomized Rats ◽  
Intact Rats

The possible direct effect of corticosterone on release of pituitary prolactin was examined in a system using incubation for 8 h. Corticosterone at either 0·1 or 1 μg/ml medium had no significant effect on in-vitro prolactin release but 10 or 100 μg/ml medium produced a significant inhibition of release of prolactin. Release of LH, FSH and thyroid-stimulating hormone were not altered by 0·1, 1 or 10 μg corticosterone/ml, indicating that its action at the concentration of 10 μg/ml was specific on release of prolactin. Corticosterone injected at doses of 1 or 5 mg/kg into hypophysectomized rats with two pituitary grafts underneath the kidney capsule produced a significant fall in serum levels of prolactin when compared with control hypophysectomized rats with two pituitary grafts. Examination with the electron microscope showed that about one third of the lactotrophes from adrenalectomized rats after corticosterone injection exhibited patterns which suggested a decrease in protein synthesis when compared with lactotrophes from adrenalectomized rats given only the vehicle injection. These observations indicated that inhibition of release of prolactin by corticosterone could be exerted directly on the pituitary gland, and that the rise of serum levels of prolactin after adrenalectomy might have been due to the removal of direct inhibition by corticosterone. Male rats were adrenalectomized and 2–3 weeks later, concentrations of dopamine and noradrenaline in the medial basal hypothalamus were measured and found not to be different from values in intact rats. Dopamine metabolism also was not altered in the median eminence. The dopaminergic agonist, l-DOPA, inhibited, and the antagonists, pimozide and haloperidol, stimulated release of prolactin in both adrenalectomized and intact rats. Serotonin (5-HT) metabolism in the medial basal hypothalamus and anterior hypothalamus of adrenalectomized rats was not significantly different from values in intact rats, but a higher concentration of 5-HT was observed in the medial basal hypothalamus of adrenalectomized rats when compared with the values in intact rats. A serotonergic agonist, fluoxetine, and an antagonist, cyproheptadine, had no apparent effect on release of prolactin in intact rats, but fluoxetine produced a significant rise, and cyproheptadine, a significant lowering of serum levels of prolactin in adrenalectomized rats. These results suggest that 5-HT, but not dopamine, may be involved in the rise of prolactin after adrenalectomy.

scholarly journals The Effect ofVitis viniferaL. Juice on Serum Levels of Inhibin B, Sperm Count in Adult Male Rats

2015 ◽  
Vol 33 (2) ◽  
pp. 109 ◽  
Author(s):  
Mohammad Reza Afzalzadeh ◽  
Akram Ahangarpour ◽  
Ashraf Amirzargar ◽  
Mohammad Kazemi Varnamkhasti ◽  
Hadi Ganjalidarani
Keyword(s):  
Adult Male ◽  
Sperm Count ◽  
Serum Levels ◽  
Inhibin B ◽  
Male Rats

EFFECT OF BILATERAL CRYPTORCHIDISM UPON PITUITARY AND SERUM LEVELS OF LH AND FSH IN SEXUALLY MATURING MALE RATS

1975 ◽  
Vol 80 (1_Suppla) ◽  
pp. S87
Author(s):  
M. Eichner ◽  
K. Rager ◽  
A. Attanasio ◽  
D. Gupta
Keyword(s):  
Serum Levels ◽  
Male Rats ◽  
Bilateral Cryptorchidism

REPRODUCTIVE TOXICITY OF CHLORPYRIFOS, CYPERMETHRIN AND THEIR INTERACTION IN MALE ALBINO RATS

2018 ◽  
pp. 69-86
Author(s):  
Asmaa ELnamaky ◽  
Amal Halawa ◽  
Mamdouh Abouelmaged
Keyword(s):  
Reproductive Toxicity ◽  
Prostatic Acid Phosphatase ◽  
Serum Levels ◽  
Reproductive Organs ◽  
Male Rats ◽  
Albino Rats ◽  
Glutathione S Transferase ◽  
Significant Drop ◽  
Sperm Abnormalities ◽  
Pituitary Glands

he present work was designed to investigate the reproductive toxicity induced by oral administration of chlorpyrifos (CPF), cypermethrin (CYP) and their combination in adult male albino rats. Forty mature male albino rats were separated into four groups (10 each), the first group was used as control, while second, third and fourth groups received orally 1/20 LD50 of CPF (10 mg/kg b.wt), 1/20 LD50 of CYP (17.22 mg/kg b.wt) and 1/40 LD50 of CPF plus 1/40 LD50 of CYP (5 mg/kg b.wt CPF plus 8.61 mg/kg b.wt CYP) respectively for 26 days. The results revealed that exposure to CPF and/or CYP induced a significant decrease in the reproductive organs weight. Moreover, a significant decrease in spermatic picture (sperm cell concentration and viability) was observed with high percent of sperm abnormalities. Serum levels of testosterone and pituitary gonadotropins (FSH and LH) have been declined significantly in all treated groups. Significant elevations were observed in malondialdehyde and nitric oxide concentrations, while antioxidant enzymes superoxide dismutase and glutathione-S-transferase activities were decreased significantly as a result of induced oxidative stress. A significant drop in prostatic acid phosphatase activity was observed. Additionally, the results showed some histopathological alterations in the reproductive organs as well as neurological lesions in brain and pituitary glands. In conclusion, CPF and CYP induce deleterious effects on reproductive efficiency of male rats which reflect more obvious impacts when both combined

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