Immunocytochemical localization of the nuclear 3,5,3'-triiodothyronine receptor in the adult rat: liver, kidney, heart, lung and spleen

1989 ◽  
Vol 120 (4) ◽  
pp. 451-458 ◽  
Author(s):  
M. Luo ◽  
R. Faure ◽  
Y. A. Tong ◽  
J. H. Dussault
Keyword(s):  
Ascitic Fluid ◽  
Cell Types ◽  
Type Ii ◽  
Myocardial Cells ◽  
Cell Nuclei ◽  
Macrophage Cell ◽  
Specific Staining ◽  
Adult Rat ◽  
Type Ii Pneumocytes ◽  
Mature Lymphocyte

Abstract. A monoclonal antibody was used for the localization of the nuclear T3 receptor in different tissues of the adult rat: the liver, kidney, heart, lung, spleen, testis, and pituitary. In the liver, the immunoreactivity was found uniformly distributed in the nuclei of hepatocytes. Sections incubated with a control ascitic fluid or with the same ascitic fluid pre-adsorbed with purified receptor showed no specific staining. In the kidney, the immunoreactivity was higher in the epithelial cell of the proximal convoluted tubes and juxtaglomerular cells. In the heart, only the myocardial cells were stained. In the lung, the immunoreactivity was confined to type II pneumocytes and alveolar macrophages. In the spleen, only a few mature lymphocyte and macrophage cell nuclei were stained. These results show that: 1) the abundance of the nuclear T3 correlates with previous studies using hormone binding techniques; 2) the nuclear T3 receptor is selectively located in certain cell types, which possess a precise local function.

Repopulation of a human alveolar matrix by adult rat type II pneumocytes in vitro

1986 ◽  
Vol 162 (2) ◽  
pp. 423-435 ◽  
Author(s):  
Jamson S. Lwebuga-Mukasa ◽  
David H. Ingbar ◽  
Joseph A. Madri
Keyword(s):  
Type Ii ◽  
Adult Rat ◽  
Type Ii Pneumocytes

Activities of enzymes of phospholipid and fatty acid synthesis in fetal and adult rat type II pneumocytes

1988 ◽  
Vol 962 (2) ◽  
pp. 173-177 ◽  
Author(s):  
Mitchell J. Kresch ◽  
Douglas A. Smart ◽  
Christine M. Wilson ◽  
Ian Gross ◽  
Seamus A. Rooney
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Synthesis ◽  
Acid Synthesis ◽  
Type Ii ◽  
Adult Rat ◽  
Type Ii Pneumocytes

The proliferative effects of retinoic acid on primary cultures of adult rat type II pneumocytes depend upon cell density

2009 ◽  
Vol 46 (1) ◽  
pp. 20-27 ◽  
Author(s):  
Richard C. Baybutt ◽  
Brendon W. Smith ◽  
Elena V. Donskaya ◽  
Ling Hu ◽  
Ting Li ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Cell Density ◽  
Primary Cultures ◽  
Type Ii ◽  
Adult Rat ◽  
Type Ii Pneumocytes

scholarly journals Uptake and subcellular distribution of Escherichia coli lipopolysaccharide by isolated rat type II pneumocytes.

1991 ◽  
Vol 39 (5) ◽  
pp. 607-615 ◽  
Author(s):  
C Risco ◽  
J L Carrascosa ◽  
M A Bosch
Keyword(s):  
Escherichia Coli ◽  
Structural Changes ◽  
Initial Period ◽  
Freeze Fracture ◽  
Type Ii ◽  
Membrane Structures ◽  
Specific Staining ◽  
Type Ii Pneumocytes ◽  
Escherichia Coli Lipopolysaccharide ◽  
Isolated Rat

Treatment of isolated rat Type II pneumocytes with Escherichia coli lipopolysaccharide (LPS) induces a number of ultra-structural changes which become evident after 60 min of incubation. By using post-embedding immunolabeling methods and electron microscopy, we have followed the fate of LPS after different times of incubation. After an initial period of accumulation in the pneumocyte microvilli, the LPS molecules enter the cytoplasm, forming discrete patches which are dispersed in some areas. After longer incubation times, LPS localize in condensed chromatin-free areas inside the nuclei. LPS micelles were visualized after freeze-fracture and compared with the LPS-labeled membrane areas, showing that LPS micelles aggregate in particular membrane zones. The sugar-specific staining in microvilli areas, where Maclura pomifera agglutinin (MPA)-gold particles bind, indicates the presence of galactose derivatives in these membrane structures. Pre-treatment of pneumocytes with LPS inhibited the MPA-gold labeling, suggesting a relation between the MPA receptor and a possible LPS receptor. Finally, double immunolabeling experiments indicated an apparent LPS-tubulin association in some particular membrane regions, which could not be observed when LPS and actin were co-localized.

DNA distribution analysis of type II pneumocytes by laser flow cytometry: technical considerations

1991 ◽  
Vol 261 (4) ◽  
pp. L296-L306 ◽  
Author(s):  
B. D. Uhal ◽  
D. E. Rannels
Keyword(s):  
Cell Cycle ◽  
Thymidine Incorporation ◽  
S Phase ◽  
Phase Fraction ◽  
Cycle Phase ◽  
Distribution Analysis ◽  
Type Ii ◽  
Adult Rat ◽  
Type Ii Pneumocytes ◽  
M Phase

Optimal conditions were established for determination of cell cycle phase fractions of freshly isolated or cultured adult rat type II pneumocytes (T2P). Propidium iodide staining of ethanol-fixed cells treated with ribonuclease (RNase) consistently yielded histograms with low coefficients of variation. Contaminating cells and cell clumps were eliminated during data acquisition through electronic gating based on anti-vimentin immunofluorescence and peak red fluorescence, respectively. Failure to delete contaminants, clumps or RNA resulted in overestimation of S or G2/M phase fractions by as much as 20-fold. When T2P were cultured on plastic at an initial density of 2.5 x 10(5)/cm2, the S phase fraction did not change over a culture interval in which thymidine incorporation rates increased almost 10-fold. In contrast, a significant increase in the G2/M phase fraction by day 2 of culture occurred with no significant increase in cell number. These results support the hypothesis that adult rat T2P, when subjected to customary conditions of primary culture, undergo cell cycle block in G2/M phases. The data also indicate that under these in vitro conditions, net thymidine incorporation by T2P may vary independently of the S phase fraction. The methods described in this report address basic considerations crucial to future applications of flow cytokinetics to the study of T2P proliferation and differentiation.

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