Ketoconazole inhibits cAMP generation and ACTH secretion in rat anterior pituitary cell culture

1987 ◽  
Vol 116 (3_Suppl) ◽  
pp. S32-S33 ◽  
Author(s):  
G. K. STALLA ◽  
J. STALLA ◽  
M. HUBER ◽  
O. A. MÜLLER
Keyword(s):  
Cell Culture ◽  
Anterior Pituitary ◽  
Pituitary Cell ◽  
Acth Secretion ◽  
Anterior Pituitary Cell ◽  
Camp Generation

scholarly journals The Pulsatility of ACTH Secretion in the Rat Anterior Pituitary Cell Perifusion System

2017 ◽  
Vol 41 (1) ◽  
pp. 154-162
Author(s):  
Qiong Deng ◽  
Zeng Zhang ◽  
Yong Wu
Keyword(s):  
Anterior Pituitary ◽  
Physiological Mechanism ◽  
Fold Increase ◽  
Pituitary Cell ◽  
Male Rats ◽  
Pituitary Cells ◽  
Acth Secretion ◽  
Physiological Concentration ◽  
Anterior Pituitary Cell ◽  
Perifusion System

Aims: This study aimed to examine the physiological mechanism whereby the corticotropin-releasing hormone (CRH) and arginine vasopressin (AVP) exert their influence on adrenocorticotropic hormone (ACTH) secretion in pituitary cells. Methods: Anterior pituitary cells were harvested from male rats and placed in the perifusion system. Cells were perifused with serum-free medium for 6 hours before fraction collection. After 30-minute of baseline collection, perifusion media was changed to expose the cells to CRH with or without AVP. ACTH concentration in each fraction was measured using enzyme immunoassay chemiluminescent kit. Results: The lowest physiological concentration of CRH (10 pM) or AVP (10 pM) was not able to induce a marked increase in ACTH secretion. Higher concentration of CRH (30 pM) or AVP (100 pM) in the physiological range caused sustained elevation of ACTH secretion (P < 0.001), while the secretion remained at similar levels for up to 1 hour with continuous stimulation. Perifusion with 10 pM AVP and 10 pM or 30 pM CRH caused a 2.38-fold and 2.99-fold increase in pulsatile ACTH secretion in pituitary cells, respectively. The duration of pulsatility caused by perifusion with 10 pM AVP and 30 pM CRH was close to that observed under physiological condition. Conclusions: By using the rat anterior pituitary cell perifusion system, we found that CRH and AVP potentiate the effect of each other on ACTH secretion, but AVP was a less potent agonist than CRH. The data suggest that CRH and AVP are essential for the pulsatility of ACTH, and AVP acts like a switch of the pulsatility.

scholarly journals Binding of 2-hydroxyestradiol to rat anterior pituitary cell membranes.

1980 ◽  
Vol 255 (20) ◽  
pp. 9838-9843
Author(s):  
J.M. Schaeffer ◽  
S. Stevens ◽  
R.G. Smith ◽  
A.J. Hsueh
Keyword(s):  
Anterior Pituitary ◽  
Cell Membranes ◽  
Pituitary Cell ◽  
Anterior Pituitary Cell

scholarly journals Anterior Pituitary Cell Antibodies Detected in Hashimoto's Thyroiditis and Graves' disease.

1988 ◽  
Vol 35 (5) ◽  
pp. 705-708 ◽  
Author(s):  
ISAO KOBAYASHI ◽  
TOSHIHIKO INUKAI ◽  
MASAKI TAKAHASHI ◽  
AKIRA ISHII ◽  
KIHACHI OHSHIMA ◽  
...  
Keyword(s):  
Hashimoto’S Thyroiditis ◽  
Anterior Pituitary ◽  
Pituitary Cell ◽  
Hashimoto's Thyroiditis ◽  
Graves Disease ◽  
Anterior Pituitary Cell
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