Influence of age, strain and season on diurnal periodicity of thyroid stimulating hormone, thyroxine, triiodothyronine and parathyroid hormone in the serum of male laboratory rats

1983 ◽  
Vol 102 (3) ◽  
pp. 377-385 ◽  
Author(s):  
Chun-Cheung Wong ◽  
Klaus-D. Döhler ◽  
Michael J. Atkinson ◽  
Heinz Geerlings ◽  
Rolf-Dieter Hesch ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Serum Levels ◽  
Thyroid Stimulating Hormone ◽  
Diurnal Periodicity ◽  
Diurnal Pattern ◽  
Sprague Dawley ◽  
Hormone Levels ◽  
Laboratory Rats ◽  
Sd Rats ◽  
Serum Hormone

Abstract. The influence of age, strain and season on the diurnal pattern of serum hormone levels from the pituitary-thyro-parathyroid complex was studied in male laboratory rats. Distinct 24 h periodicity in the serum levels of thyroid stimulating hormone (TSH) and triiodothyronine (T3) was observed in all groups of rats. There was no influence of age (40, 60 and 90 days old Sprague-Dawley rats), but a significant influence of strain (Sprague-Dawley vs. BH/Ztm rats) and season (summer vs. winter) on the diurnal pattern of serum TSH and T3 levels. Significant 24 h periodicity in serum thyroxine (T4) levels existed during winter in BH/Ztm rats, but not in Sprague-Dawley (SD) rats of any age. Adult SD rats demonstrated 24 h periodicity in serum levels of T4 only in summer. No diurnal periodicity in serum levels of parathyroid hormone (PTH) was observed in any group of rats. There were significant changes in 24 h mean serum levels of TSH and T3 throughout pubertal development. Twenty-four h mean serum levels of T3 and T4 were significantly higher in summer than in winter. Twenty-four h mean serum levels of T4 were significantly lower in BH/Ztm rats than in SD rats. Significant correlation was observed between serum concentrations of T3 and T4, TSH and T4, and between TSH and T3 in some groups of rats, but not in all. The results indicate that 24 h periodicity of serum hormone levels from the pituitary-thyroid complex of male laboratory rats may vary with age and strain of the animals and with the season of experiment performance.

Steroid Hormone Levels in Patients with Advanced Breast Cancer during Therapy with Droloxifene: A Pilot Study

1996 ◽  
Vol 82 (1) ◽  
pp. 45-47
Author(s):  
Jacqueline Dalski ◽  
Max Görlich
Keyword(s):  
Breast Cancer ◽  
Advanced Breast Cancer ◽  
Tumor Progression ◽  
Postmenopausal Women ◽  
Serum Levels ◽  
Dehydroepiandrosterone Sulfate ◽  
Advanced Breast ◽  
Hormone Levels ◽  
Serum Hormone ◽  
Early Indication

Antiestrogens, particularly tamoxifen, are effective in the treatment of pre- and postmenopausal women suffering from all stages of breast cancer. Unfortunately, many patients become resistant to tamoxifen during therapy, which allows the tumor to progress. Thus, a preclinical recognition of tumor progression, i.e. by monitoring serum hormone levels, could be worthwhile. The serum levels of dehydroepiandrosterone sulfate and estradiol of postmenopausal women with advanced breast cancer treated by the new antiestrogen droloxifene were therefore checked. However, only non-significant changes in the hormone levels during droloxifene therapy were observed, and no relation was found between hormone levels and the course of the disease, success or exhaustion of droloxifene application, or development of tumor progression. Our data do not confirm earlier findings reported in the literature that measurement of hormones seems to be suitable for an early indication of tumor progression during an antiestrogen therapy before its clinical manifestation.

scholarly journals Improvement of calcium balance by Fructus Ligustri Lucidi extract in mature female rats was associated with the induction of serum parathyroid hormone levels

2011 ◽  
Vol 108 (1) ◽  
pp. 92-101 ◽  
Author(s):  
Xiao-Li Dong ◽  
Ming Zhao ◽  
Kwun-Kit Wong ◽  
Chun-Tao Che ◽  
Man-Sau Wong
Keyword(s):  
Parathyroid Hormone ◽  
Bone Mineral ◽  
Soluble Fraction ◽  
Mature Female ◽  
Female Rats ◽  
Sprague Dawley ◽  
Serum Parathyroid Hormone ◽  
Hormone Levels ◽  
Fructus Ligustri Lucidi ◽  
Different Levels

Fructus Ligustri Lucidi (FLL) is a commonly prescribed herb in many kidney-tonifying Traditional Chinese Medicinal formulae for the treatment of osteoporosis. The present study aimed to identify the active fractions in FLL and to characterise its effects on Ca balance, calciotropic hormone levels as well as bone properties in mature female rats fed diets containing different levels of Ca. In the present study, 4-month-old Sprague–Dawley female rats were treated with either FLL ethanol extract (EE), ethyl acetate-soluble fraction of EE (EAF), water-soluble fraction of EE (WF) or their vehicle for 12 weeks on a medium-Ca diet (MCD, 0·6 % Ca, 0·65 % P). Then, the Sprague–Dawley female rats treated with WF or its vehicle for 12 weeks were fed diets containing different levels of dietary Ca (low-Ca diet (LCD), 0·1 % Ca, 0·65 % P; MCD; high-Ca diet (HCD), 1·2 % Ca, 0·65 % P). The results demonstrated that WF from EE but not EAF exerted a prominent effect on Ca balance by inhibiting urinary and faecal Ca excretion. WF significantly increased Ca balance in rats fed MCD or HCD with an associated increase in serum parathyroid hormone (PTH) levels. WF did not alter bone mineral density or bone mineral content of the tibia in all the rats fed with different levels of dietary Ca. In conclusion, WF was responsible for the positive actions of FLL on Ca absorption and balance. The regulation of Ca balance by WF might involve its action in stimulating PTH production in the mature female rats.

scholarly journals Sex Hormones Enhance Gingival Inflammation without Affecting IL-1βand TNF-αin Periodontally Healthy Women during Pregnancy

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Min Wu ◽  
Shao-Wu Chen ◽  
Wei-Lan Su ◽  
Hong-Ying Zhu ◽  
Shu-Yuan Ouyang ◽  
...  
Keyword(s):  
Gingival Crevicular Fluid ◽  
Interleukin 1 ◽  
Serum Levels ◽  
Hormonal Changes ◽  
Gingival Inflammation ◽  
Pocket Depth ◽  
Hormone Levels ◽  
Probing Pocket Depth ◽  
Inflammatory Status ◽  
Serum Hormone

Hormones (progesterone and estradiol) change greatly during pregnancy; however, the mechanism of hormonal changes on gingival inflammation is still unclear. This study is to evaluate the effects of hormonal changes during pregnancy on gingival inflammation and interleukin-1β(IL-1β) and tumor necrosis factor-α(TNF-α) in gingival crevicular fluid (GCF). 30 periodontally healthy pregnant women were evaluated in the first, second, and third trimesters. 20 periodontally healthy nonpregnant women were evaluated twice (once per subsequent month). Clinical parameters including probing pocket depth (PPD), bleeding index (BI), gingival index (GI), clinical attachment level (CAL), and plaque index (PLI) were recorded. GCF levels of IL-1βand TNF-αand serum levels of progesterone and estradiol were measured. From the data, despite low PLI, BI and GI increased significantly during pregnancy; however, no significant changes in PLI, CAL, IL-1β, or TNF-αGCF levels were observed. Although IL-1β, not TNF-α, was higher in pregnant group than in nonpregnant group, they showed no correlation with serum hormone levels during pregnancy. GI and BI showed significant positive correlation with serum hormone levels during pregnancy. This study suggests that sex hormone increase during pregnancy might have an effect on inflammatory status of gingiva, independent of IL-1βand TNF-αin GCF.

scholarly journals Effects of Coconut Water on Retina in Diabetic Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-7 ◽  
Author(s):  
Xiaohua Zhang ◽  
Li Peng ◽  
Yanan Dai ◽  
Xia Sheng ◽  
Shaomei Chen ◽  
...  
Keyword(s):  
Serum Levels ◽  
Underlying Mechanism ◽  
Diabetic Rats ◽  
Biologically Active ◽  
Coconut Water ◽  
Intercellular Adhesion Molecule ◽  
Sprague Dawley ◽  
Intercellular Adhesion Molecule 1 ◽  
Sd Rats ◽  
Healthy Adult Male

Coconut water (CW) is a natural aseptic nutritious beverage, containing several biologically active compounds. This study aimed to determine the antiretinopathy effects of CW on diabetic Sprague Dawley (SD) rats using streptozotocin (STZ) and explore its potential mechanism. After allowing the rats to acclimatize for 7 days, 48 healthy adult male SD rats were selected and randomly divided into 4 groups, involving control (Ctrl), diabetic rats (DM), diabetic rats treated with CW (DM-CW), and diabetic rats treated with glibenclamide (DM-Gli). The diabetic models were established by an intraperitoneal injection of STZ (60 mg/kg). The Ctrl group was injected with an equal volume of sodium citrate solution. The experiment was totally conducted during 20 weeks, and then, all rats were sacrificed. The serum levels of superoxide dismutase (SOD), malondialdehyde (MDA), and glutathione peroxidase (GSH-Px) were measured; additionally, the activities of interleukin-6 (IL-6) and intercellular adhesion molecule-1 (ICAM-1) in the retina were investigated using biochemical assays. Hematoxylin and eosin (H&E) staining was performed to observe pathological changes of retinal tissues. In presence of treatment with CW, serum level of MDA was decreased, while serum levels of SOD and GSH-Px were increased; besides, the activities of IL-6 and ICAM-1 in the retina were reduced compared with the DM group. The antiretinopathy feature of CW was confirmed by the increased number of neurons in the ganglion cell layer (GCL), total retina thickness (TRT), and thickness of the retinal nuclear layer (RNL) in diabetic rats. CW can be protective against diabetic retinopathy (DR), and its effects are comparable to Gli. The possible underlying mechanism may be partly explained by decreasing oxidative stress and anti-inflammatory activities in the retina. However, further research should be conducted to reveal the exact mechanism.

scholarly journals Effects of Continuous Estradiol Administration by Polydimethylsiloxane and Paraffin Implants on Serum Hormone Levels and Uterine Responses

1984 ◽  
Vol 3 (4) ◽  
pp. 303-316
Author(s):  
D. M. Sheehan ◽  
W. S. Branham ◽  
R. Gutierrez-Cernosek ◽  
S. F. Cernosek
Keyword(s):  
High Serum ◽  
Serum Prolactin ◽  
High Dose ◽  
Continuous Exposure ◽  
Sprague Dawley ◽  
Hormone Levels ◽  
Dna And Rna ◽  
Uterine Growth ◽  
Serum Hormone ◽  
Rna Accumulation

Since tonic elevated estrogen levels are associated with toxic responses, including tumor promotion, we investigated the effects of continuous estradiol (E2) administration by paraffin or polydimethylsiloxane (Silastic) implants on pituitary and uterine responses. Ovariectomized (OVEX) Sprague-Dawley rats implanted with low-dose (P1) or high-dose (P2) paraffin pellets showed initial high serum E2 levels that decayed rapidly. Silastic implants provided more constant pharmacological serum E2 levels during the 14 days of exposure. Rising serum LH and FSH levels in OVEX controls were suppressed by all E2 implants. Serum prolactin levels, low in OVEX controls, rose after implantation (P1 and P2) but decayed to control values by 14 days. Prolactin induced by pharmacological E2 levels initially rose, dropped by 72 hours, but again rose at later times. Uterine weight, low in OVEX controls, was increased by all implants. At later times, paraffin implants did not appear to release significant amounts of E2. However, removal of P2 implants from donors followed by transfer to new recipients stimulated uterine growth in recipients. All implants increased uterine DNA and RNA levels and the RNA/DNA ratio during the first 24–48 hours, followed by maintenance of elevated values. Poly(A)-containing RNA, isolated by oligo(dT) cellulose chromatography, comprised a constant percentage of RNA at all hormone levels and treatment times, suggesting a coupling of total RNA and poly(A)-containing RNA accumulation. Results are discussed in relation to the carcinogenic properties of estrogens, known to be correlated with continuous exposure to elevated estrogen levels.

scholarly journals Effect of Acupuncture on Simple Obesity and Serum Levels of Prostaglandin E and Leptin in Sprague-Dawley Rats

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Xiaomin Li ◽  
Zijian Wu ◽  
Yenong Chen ◽  
Ronglin Cai ◽  
Zhizhen Wang
Keyword(s):  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Serum Levels ◽  
Normal Diet ◽  
The Body ◽  
Liver Fat ◽  
Prostaglandin E ◽  
Sprague Dawley ◽  
Sd Rats ◽  
Simple Obesity

Aim. The study is aimed at investigating the curative effect of acupuncture on simple obesity and its influence on serum levels of prostaglandin E and leptin in Sprague-Dawley (SD) rats. Methods. In the study, there are 50 male SD rats. We took 10 as healthy controls and fed 40 with a diet of high fat for 8 weeks. After the 40 rat model was established successfully, we fed 10 rats in the model group with a normal diet and treated 10 rats in the acupuncture group by acupuncture. During the experiment, the body fat and body length of rats were measured weekly, and Lee’s index was calculated. After the treatment, the levels of leptin, prostaglandin E, C-reactive protein (CRP), triacylglycerol (TG), cholesterol (CHO), low-density lipoprotein (LDL), and high-density lipoprotein (HDL) were detected, and the liver fat morphology was observed by electron microscope. Results. Acupuncture significantly downregulated the serum levels of CRP, TG, CHO, LDL, leptin, and prostaglandin E and upregulated the serum levels of HDL in rats with simple obesity. Conclusion. On basis of these results, it was found that acupuncture could boost fat metabolism and weight loss by inhibiting the production of leptin and prostaglandin E.

Interstitial Cells in the Pineal Gland of Pregnant and Nonpregnant Viscachas (Lagostomus maximus maximus): A Morphometric and Biochemical Study

2018 ◽  
Vol 205 (4) ◽  
pp. 240-250
Author(s):  
Fabricio Ivan Busolini ◽  
Luis Ezequiel Gallol ◽  
Graciela Beatríz Rodríguez ◽  
Verónica Palmira Filippa ◽  
Fabian Heber Mohamed
Keyword(s):  
Pineal Gland ◽  
Biochemical Study ◽  
Late Pregnancy ◽  
Serum Levels ◽  
Interstitial Cells ◽  
Gonadal Hormone ◽  
Hormone Levels ◽  
Serum Hormone ◽  
S 100 ◽  
Percentage Area

The pineal gland of mammals undergoes morphological and biochemical changes throughout the gestation period. In viscachas, a seasonal breeding rodent, pregnancy lasts approximately 154 days and 3 stages can be defined, i.e., early, mid, and late pregnancy. The purpose of this study is to analyze morphometric variations in the expression of S-100 protein, glial fibrillary acidic protein (GFAP), and vimentin in the interstitial cells (IC) in pregnant and nonpregnant viscachas by immunohistochemistry (IHC). We also aim to evaluate a probable relation between glandular activity and pregnancy. The immunopositive percentage area (%IA) for the studied proteins and the number of immunoreactive cells against the S-100 protein with a visible nucleus (nº IC-S-100) were analyzed. Estradiol and progesterone serum levels were also determined by RIA. Variations in the expression of the S-100 protein and GFAP, as well as changes in the nº IC-S-100 related to serum hormone levels, were found between pregnant and nonpregnant viscachas. Viscachas in mid pregnancy exhibited the highest values of %IA for the analyzed proteins, followed by females in late and early pregnancy, while the nonpregnant ones showed the lowest values for all of the groups studied. Likewise, the nº IC-S-100 also varied following the same pattern. Thus, these variations seem to indicate a direct relationship between glandular activity and gonadal hormone levels. On these grounds, we may conclude that IC undergo changes in relation to ovarian hormone levels and participate in the regulation of glandular activity during pregnancy. However, further research is necessary to elucidate this relationship.

Circannual variations in serum concentrations of pituitary, thyroid, parathyroid, gonadal and adrenal hormones in male laboratory rats

1983 ◽  
Vol 97 (2) ◽  
pp. 179-185 ◽  
Author(s):  
C. C. Wong ◽  
K.-D. Döhler ◽  
M. J. Atkinson ◽  
H. Geerlings ◽  
R.-D. Hesch ◽  
...  
Keyword(s):  
Serum Levels ◽  
Seasonal Effects ◽  
Serum Concentrations ◽  
Sprague Dawley ◽  
Adrenal Hormones ◽  
Laboratory Rats ◽  
Laboratory Conditions ◽  
Sprague Dawley Rats ◽  
Old Rats ◽  
Observation Period

Seasonal effects were studied on basal levels of hormones in the serum of adult male Sprague–Dawley rats, which were born and raised under rigorously controlled laboratory conditions. Groups of 90-day-old rats were killed at monthly intervals by rapid decapitation. Significant fluctuations were observed throughout the observation period of 19 months in serum levels of TSH, prolactin, androgens, tri-iodothyronine and LH. Minor fluctuations were observed in serum levels of FSH, corticosterone, parathyroid hormone and thyroxine. The results indicate that male laboratory rats exhibit circannual and semi-annual fluctuations in serum levels of several hormones even though the animals were born, raised and maintained in constant laboratory conditions.

scholarly journals EFFECTS OF NOISE STRESS ON THYROID STIMULATING HORMONE IN ADULT RATS

2021 ◽  
Vol 71 (4) ◽  
pp. 1157-60
Author(s):  
Saba Saleem Safdar ◽  
Khadija Qamar ◽  
Khadija Mehboob ◽  
Tayyaba Faisal ◽  
Fahad Atta ◽  
...  
Keyword(s):  
Hormone Level ◽  
Thyroid Stimulating Hormone ◽  
Control Group ◽  
Sprague Dawley ◽  
Serum Samples ◽  
Adult Rats ◽  
Hormone Levels ◽  
Noise Stress ◽  
Group A ◽  
Stimulating Hormone

Objective: To determine the effect of noise stress on Thyroid Stimulating Hormone in adult rats. Study Design: Laboratory-based experimental study. Place and Duration of Study: Department of Anatomy Army Medical College, Rawalpindi in collaboration with National Institute of Health, Islamabad, from Jan to Dec 2020. Methodology: A total of 30 adult Sprague Dawley rats were distributed into three groups as ten rats/group. Group A served as the control group, rats in group B were exposed to 100 dB noise for continuous 4 hours/day for four months, and the rats in group C were exposed to 100 dB noise for continuous 6 hours/day for four months. At the end of the study, serum samples were collected from the rats’ tail veins. Thyroid-stimulating hormone levels were compared among the groups. Results: At the start of the study, mean serum thyroid stimulating hormone level of control group A rats was 0.34 ± 0.15 µIU/ml. Mean serum thyroid stimulating hormone level of experimental groups B and C rats at the start of the study was 0.46 ± 0.18 µIU/ml and 0.41 ± 0.14 µIU/ml, respectively Mean serum thyroid stimulating hormone of rats did not differ statistically significantly between the group p=0.26, By the end of study mean serum thyroid stimulating hormone level of control group. A rats was 0.6 ± 0.2 µIU/ml. Mean serum thyroid stimulating hormone levels of experimental groups B and C rats at the end of the study were 1.32 ± 0.35 µIU/ml and 1.86 ± 0.67 µIU/ml, respectively.......

Effect of acupuncture on menopausal hot flushes and serum hormone levels: a systematic review and meta-analysis

2021 ◽  
pp. 096452842110566
Author(s):  
Chang Liu ◽  
Zhijie Wang ◽  
Ting Guo ◽  
Lixing Zhuang ◽  
Xiao Gao
Keyword(s):  
Meta Analysis ◽  
Serum Levels ◽  
Sham Acupuncture ◽  
Hot Flushes ◽  
Hormone Levels ◽  
Hot Flush ◽  
Menopausal Women ◽  
Serum Hormone ◽  
Meta Analyses ◽  
Review Manager

Objective: To evaluate the efficacy/effectiveness and safety of acupuncture for the treatment of hot flushes and its impact on serum hormone levels in menopausal women. Methods: A total of 10 databases were searched from their inception to August 2018. Reference lists of reviews and included articles were also hand-searched. Randomized controlled trials (RCTs) comparing the effect of acupuncture versus sham acupuncture, or acupuncture versus hormone therapy (HT), as treatment for menopausal hot flushes were included. Outcomes included hot flush frequency, hot flush severity and serum hormone levels of estradiol (E2), luteinizing hormone (LH) and follicle-stimulating hormone (FSH). Meta-analyses were performed using Review Manager 5.3 software. Results: Thirteen RCTs including 1784 patients were selected, seven of which were available for meta-analysis. Compared with sham acupuncture, acupuncture significantly decreased hot flush frequency (mean difference (MD) −0.84, 95% confidence interval (CI) [−1.64, −0.05], I2 = 54%) from baseline to the end of study, but did not impact end scores of hot flush frequency (MD 0.19, 95% CI [−0.61, 0.99], I2 = 0%) or severity (MD 0.02, 95% CI [−0.13, 0.17], I2 = 0%). No differences were found between acupuncture and HT in serum levels of E2 (MD 6.56, 95% CI [−3.77, 16.89], I2 = 76%), FSH (MD 1.06, 95% CI [−1.44, 3.56], I2 = 0%) or LH (MD −3.36, 95% CI [−13.37, 6.65], I2 = 89%). Conclusion: Acupuncture may not decrease hot flush frequency, but yet appears to have similar effects on serum hormone levels as HT, that is, increased E2 and decreased FSH and LH. Considering that no firm conclusions could be drawn due to the low quality and limited number of included trials included, further high-quality RCTs need to be conducted.

Sign in / Sign up

Export Citation Format

Share Document