Influence of corticotrophin on plasma testosterone in normal women

1980 ◽  
Vol 94 (2) ◽  
pp. 201-203 ◽  
Author(s):  
A. Vérine ◽  
E. Trouvé-Blanqui ◽  
C. Oliver ◽  
J. Boyer
Keyword(s):  
Plasma Level ◽  
Therapeutic Agent ◽  
Mean Value ◽  
Plasma Testosterone ◽  
Acth Level ◽  
The Mean ◽  
Normal Women

Abstract. The concentrations of ACTH and testosterone (T) in the plasma from 12 normal women (aged 18–35 years) were simultaneously determined at various times after im injection of 1 mg corticotrophin. The increase in the plasma level of total immunoreactive ACTH was maximal 0.5 h after the injection (mean value 1123 as compared to 36 pg/ml in the basal state); at 48 h, the ACTH level was still 2.4-fold that measured in the basal state. The administration of corticotrophin induced a broad increase in the mean level of plasma T which was highly significant (P < 0.001) at 4, 6, 8, 16 and 24 h after the injection. The maximum T response was detected at 16 h (mean value 477 as compared to 338 pg/ml in the basal state). The results indicate that plasma T is susceptible to stimulation by a pharmacological dose of ACTH in normal women. This effect should be taken into account in evaluating the indication of ACTH as a therapeutic agent.

PLASMA ANDROGENS IN WOMEN

1964 ◽  
Vol 45 (2) ◽  
pp. 254-271 ◽  
Author(s):  
W. J. Dignam ◽  
R. J. Pion ◽  
E. J. Lamb ◽  
H. H. Simmer
Keyword(s):  
Clinical Manifestations ◽  
Unknown Origin ◽  
Mean Value ◽  
Significant Rise ◽  
Plasma Testosterone ◽  
List Type ◽  
Polycystic Ovaries ◽  
Significant Drop ◽  
Mean Values ◽  
Normal Women

ABSTRACT Plasma levels of conjugated DHEA, conjugated androsterone (modified method of Migeon & Plager) and testosterone (method of Finkelstein et al.) were determined in a group of 20 patients with polycystic ovaries and hirsutism or virilism, before and after intravenous HCG stimulation and ACTH-suppression with dexamethasone. Mean values for testosterone, conjugated DHEA and androsterone were.34 ±.24 μg/100 ml, 50.9 ± 28.3 μg/100 ml and 31.4 ± 19.4 μg/100 ml respectively, all significantly higher than levels found in normal women. In all, but 3 cases, testosterone values were above the 2-sigma range of normal women in contrast to the frequent overlapping observed for conjugated DHEA and androsterone. HCG stimulation led to a significant rise in the mean value of testosterone (.61 ±.22 μg/100 ml) but little change was seen in those of the conjugates. While dexamethasone caused a marked decrease of testosterone in only 2 patients, it led to a pronounced fall in DHEA values and a lesser but significant drop in androsterone concentrations in most patients. In patients with a unilateral polycystic or with bilateral polycystic ovaries but without hirsutism or virilism similar values were noted. Two patients with arrhenoblastomas, 4 patients with adrenal disorders and 13 patients with hirsutism of unknown origin were studied and their findings presented. All patients with hirsutism or virilism had a higher mean value of testosterone than did normal women. The testosterone levels did not parallel the severity, although a correlation did seem to exist in the mildly and moderately hirsute patients. Plasma androgen determinations in general and plasma testosterone studies, in particular, while helpful in elucidating the clinical manifestations of masculinization do not allow the investigator to differentiate an adrenal from an ovarian source. Adjunctive studies, utilizing HCG and dexamethasone, are helpful, but not conclusive.

The quantitation of thioredoxin 1 from serum as a novel means to detect breast cancer (BC).

2020 ◽  
Vol 38 (15_suppl) ◽  
pp. 557-557
Author(s):  
Kyoung Hoon Suh
Keyword(s):  
Breast Cancer ◽  
Histological Grade ◽  
Accurate Method ◽  
Mean Value ◽  
Test Results ◽  
Thioredoxin 1 ◽  
The Mean ◽  
Normal Women ◽  
Detect Breast Cancer ◽  
One Way Anova

557 Background: Breast cancer (BC) is the most common cancer and the second leading cause of cancer death in women. Enormous effort has been conducted without success to develop a means to detect BC using the blood. We have reported that the level of thioredoxin 1 (Trx1) in serum could be a novel standard to evaluate the risk of BC. Therefore, we have investigated the clinical utility of Trx1 as a biomarker to detect BC by testing sera from normal women, women with BC, women with five other types of cancer. Methods: We have developed an ELISA kit that quantitates Trx1 in sera. The level of Trx1 was determined in each serum from normal healthy women (n = 114), as well as patients with BC (n = 106), cervical cancer (n = 17), lung cancer (n = 14), stomach cancer (n = 9), and thyroid cancer (n = 4). BC patients were recruited according to their age and cancer stage. Each test was duplicated more than three times, and test results were analyzed by ROC analysis, one-way ANOVA tests, and unpaired t-tests. Results: The mean value of Trx1 from normal women was 5.60±4.39(±SD) and that from BC was 22.25±7.07. The Trx1 level was effective to distinguish BC serum from healthy serum with a sensitivity of 94.3% and specificity of 93.9% (AUC 0.985, p< 0.001). The levels of Trx1 from BC patients were higher than the cut-off value of 14.13 U/ml regardless of age, stage, histological grade, type, ER/PR/HER2 expression profile, and proliferation activity of BC cells. The levels of Trx1 from the other five types of cancers (2.34±1.82 - 3.64±2.99) were low enough to be distinguishable from BC. Especially, Trx1 levels could rescue patients whose mammography resulted in a false judgement. Conclusions: These results indicated that the blood level of Trx1 is an effective and accurate method to detect breast cancer, and particularly as a complement to mammography.

THE EFFECT OF MESTEROLONE ADMINISTRATION TO NORMAL MEN ON THE PITUITARY-TESTICULAR FUNCTION

1974 ◽  
Vol 77 (2) ◽  
pp. 380-386 ◽  
Author(s):  
A. Aakvaag ◽  
S. B. Strømme
Keyword(s):  
Protein Binding ◽  
Testosterone Level ◽  
Plasma Levels ◽  
Mean Value ◽  
Plasma Testosterone ◽  
Testicular Function ◽  
Plasma Testosterone Level ◽  
Before And After ◽  
The Mean ◽  
Normal Men

ABSTRACT Mesterolone (1α-methyl-5α-dihydrotestosterone) has been given to 10 normal men, age 24–27 years, and the effect on the plasma levels of ICSH, FSH and testosterone has been studied. No effect on the plasma levels of ICSH and FSH could be detected. After 4 weeks on 75 mg mesterolone per day a significant (P < 0.01) drop in the mean value for plasma testosterone level was observed, 5.2 to 4.0 ng/ml. After another 4 weeks on 150 mg mesterolone per day a further decrease to 3.5 ng/ml was found. During mesterolone administration the protein binding of testosterone in plasma was significantly reduced, and it appeared that the level of free (non-protein bound) testosterone in diluted plasma remained unchanged, 0.37 and 0.41 ng/ml, before and after mesterolone administration respectively. The results suggest that mesterolone given in doses of 75 and 150 mg/day to normal men does not suppress the pituitary ICSH production or the testicular testosterone production.

HYPOPHYSO-GONADAL FUNCTION IN THE CRYPTORCHID CHILD: DIFFERENCES BETWEEN UNILATERAL AND BILATERAL CRYPTORCHIDS

1976 ◽  
Vol 83 (1) ◽  
pp. 182-189 ◽  
Author(s):  
E. Cacciari ◽  
A. Cicognani ◽  
P. Pirazzoli ◽  
F. Zappulla ◽  
P. Tassoni ◽  
...  
Keyword(s):  
Bone Age ◽  
Mean Value ◽  
Plasma Testosterone ◽  
Control Group ◽  
Gonadal Function ◽  
Normal Children ◽  
Before And After ◽  
The Mean ◽  
Lh Rh ◽  
Plasma Oestradiol

ABSTRACT In 22 normal boys, 33 unilateral and 14 bilateral cryptorchids, a gonadal function test (2000 IU of HCG im each a day for three days and assays of plasma testosterone and plasma oestradiol-17β before and after the HCG administration) as well as an LH-RH test were carried out. In 60% of the cases, both normal and cryptorchid boys, plasma oestradiol-17β (both in basal conditions and after stimulus) were found to be less than the sensitivity (5 pg/ml) of the method. While the plasma testosterone was similar under basal conditions in the three groups of children, after HCG it was significantly lower than the mean value of the control group only in the bilateral cryptorchids. The testosterone levels, both under basal conditions and after stimulus, are correlated to bone age only in the normal boys and in the unilateral cryptorchids. There were no significant differences among the various groups for either LH and FSH both under basal conditions and after LH-RH. The LH curve area during the LH-RH test is in correlation with bone age only in the normal children.

THE EFFECT OF EPINEPHRINE ON TESTOSTERONE PRODUCTION

1967 ◽  
Vol 55 (1) ◽  
pp. 184-192 ◽  
Author(s):  
Joseph Levin ◽  
Charles W. Lloyd ◽  
Julia Lobotsky ◽  
E. H. Friedrich
Keyword(s):  
Production Rate ◽  
Mean Value ◽  
Constant Infusion ◽  
Plasma Testosterone ◽  
Metabolic Clearance ◽  
Metabolic Clearance Rate ◽  
Testosterone Concentration ◽  
The Mean ◽  
Male Subjects ◽  
Double Isotope

ABSTRACT The metabolic clearance rate (MCR) and the production rate (PR) of testosterone were measured in four male subjects by the method of constant infusion of tritiated testosterone. The mean value of the MCR of 1161 ± 80 (SD) liters/24 hours was not altered by the infusion of epinephrine, at the rate of 0.466 mg per hour for three: hours. The plasma testosterone concentration was measured by the double isotope method of Riondel et al. (1963). Epinephrine significantly decreased this concentration (28%) and also the production rate (28%) The effect of epinephrine on plasma testosterone concentration was measured in six additional male subjects, and the results of the total of 10 subjects showed that there was a decrease of (28%) in the concentration. It was concluded that epinephrine significantly diminished the production rate of testosterone.

Long-period modulated superstructures in Pd-12.5 at.%Ce and Pd-15 at.%Ce alloys

1990 ◽  
Vol 48 (4) ◽  
pp. 164-165
Author(s):  
Noriyuki Kuwano ◽  
Masaru Itakura ◽  
Kensuke Oki
Keyword(s):  
Electron Microscopy ◽  
Mean Value ◽  
Heavy Elements ◽  
Arc Furnace ◽  
X Ray ◽  
Long Period ◽  
Ultra High Purity ◽  
Heat Treated ◽  
Atomic Scattering ◽  
The Mean

Pd-Ce alloys exhibit various anomalies in physical properties due to mixed valences of Ce, and the anomalies are thought to be strongly related with the crystal structures. Since Pd and Ce are both heavy elements, relative magnitudes of (fcc-fpd) are so small compared with <f> that superlattice reflections, even if any, sometimes cannot be detected in conventional x-ray powder patterns, where fee and fpd are atomic scattering factors of Ce and Pd, and <f> the mean value in the crystal. However, superlattices in Pd-Ce alloys can be analyzed by electron microscopy, thanks to the high detectability of electron diffraction. In this work, we investigated modulated superstructures in alloys with 12.5 and 15.0 at.%Ce.Ingots of Pd-Ce alloys were prepared in an arc furnace under atmosphere of ultra high purity argon. The disc specimens cut out from the ingots were heat-treated in vacuum and electrothinned to electron transparency by a jet method.

Partition Coefficient Ratios and Tumour Perfusion Studied with 85mKr and 133Xe

1987 ◽  
Vol 26 (06) ◽  
pp. 253-257
Author(s):  
M. Mäntylä ◽  
J. Perkkiö ◽  
J. Heikkonen
Keyword(s):  
Partition Coefficient ◽  
Partition Coefficients ◽  
Regional Blood Flow ◽  
Blood Perfusion ◽  
Compartmental Analysis ◽  
Malignant Tumour ◽  
Mean Value ◽  
Perfusion Rate ◽  
Biological Variables ◽  
The Mean

The relative partition coefficients of krypton and xenon, and the regional blood flow in 27 superficial malignant tumour nodules in 22 patients with diagnosed tumours were measured using the 85mKr- and 133Xe-clearance method. In order to minimize the effect of biological variables on the measurements the radionuclides were injected simultaneously into the tumour. The distribution of the radiotracers was assumed to be in equilibrium at the beginning of the experiment. The blood perfusion was calculated by fitting a two-exponential function to the measuring points. The mean value of the perfusion rate calculated from the xenon results was 13 ± 10 ml/(100 g-min) [range 3 to 38 ml/(100 g-min)] and from the krypton results 19 ± 11 ml/(100 g-min) [range 5 to 45 ml/(100 g-min)]. These values were obtained, if the partition coefficients are equal to one. The equations obtained by using compartmental analysis were used for the calculation of the relative partition coefficient of krypton and xenon. The partition coefficient of krypton was found to be slightly smaller than that of xenon, which may be due to its smaller molecular weight.

Detection of Soluble Fibrin Monomer Complexes in Blood by Means of Protamine Sulphate Test

1968 ◽  
Vol 20 (01/02) ◽  
pp. 044-049 ◽  
Author(s):  
B Lipiński ◽  
K Worowski
Keyword(s):  
Human Subjects ◽  
Coronary Thrombosis ◽  
Mean Value ◽  
Healthy Human ◽  
Protamine Sulphate ◽  
Soluble Fibrin ◽  
Fibrin Monomer ◽  
Dog Plasma ◽  
The Mean ◽  
Precipitable Protein

SummaryIn the present paper described is a simple test for detecting soluble fibrin monomer complexes (SFMC) in blood. The test consists in mixing 1% protamine sulphate with diluted oxalated plasma or serum and reading the optical density at 6190 Å. In experiments with dog plasma, enriched with soluble fibrin complexes, it was shown that OD read in PS test is proportional to the amount of fibrin recovered from the precipitate. It was found that SFMC level in plasma increases in rabbits infused intravenously with thrombin and decreases after injection of plasmin with streptokinase. In both cases PS precipitable protein in serum is elevated indicating enhanced fibrinolysis. In healthy human subjects the mean value of OD readings in plasma and sera were found to be 0.30 and 0.11, while in patients with coronary thrombosis they are 0.64 and 0.05 respectively. The origin of SFMC in circulation under physiological and pathological conditions is discussed.

Detection of Circulating Tissue Factor and Factor VII in a Normal Population

1996 ◽  
Vol 75 (05) ◽  
pp. 772-777 ◽  
Author(s):  
Sybille Albrecht ◽  
Matthias Kotzsch ◽  
Gabriele Siegert ◽  
Thomas Luther ◽  
Heinz Großmann ◽  
...  
Keyword(s):  
Tissue Factor ◽  
Normal Population ◽  
Mean Value ◽  
Factor Vii ◽  
Significant Difference ◽  
Age Dependent ◽  
The Mean ◽  
Highly Correlated ◽  
And Gender

SummaryThe plasma tissue factor (TF) concentration was correlated to factor VII concentration (FVIIag) and factor VII activity (FVIIc) in 498 healthy volunteers ranging in age from 17 to 64 years. Immunoassays using monoclonal antibodies (mAbs) were developed for the determination of TF and FVIIag in plasma. The mAbs and the test systems were characterized. The mean value of the TF concentration was 172 ± 135 pg/ml. TF showed no age- and gender-related differences. For the total population, FVIIc, determined by a clotting test, was 110 ± 15% and the factor VIlag was 0.77 ± 0.19 μg/ml. FVII activity was significantly increased with age, whereas the concentration demonstrated no correlation to age in this population. FVII concentration is highly correlated with the activity as measured by clotting assay using rabbit thromboplastin. The ratio between FVIIc and FVIIag was not age-dependent, but demonstrated a significant difference between men and women. Between TF and FVII we could not detect a correlation.

Determination of Plasminogen in Clots and Thrombi

1966 ◽  
Vol 16 (01/02) ◽  
pp. 038-050 ◽  
Author(s):  
Ulla Hedner ◽  
Inga Marie Nilsson ◽  
B Robertson
Keyword(s):  
Mean Value ◽  
Main Mechanism ◽  
Clot Lysis ◽  
Post Mortem ◽  
Digestion Time ◽  
Normal Volunteers ◽  
Casein Solution ◽  
The Mean

SummaryThe plasminogen content was determined by a casein method in plasma and serum from 20 normal volunteers. The mean plasminogen content was found to be 10.1 ACU (the arbitrary caseinolytic unit defined in such a way that using a 3% casein solution and a digestion time of 20 min. at 37°C, 10 ACU gave an extinction of 0.300). No difference between serum and plasma regarding the plasminogen content was found.Plasminogen was determined in drained and drained plus washed clots prepared from 2 ml plasma. The highest values found in the drained clots were 0.9 ACU/clot and 0.2 ACU/clot in the drained plus washed clots.Plasminogen was also determined in drained and drained plus washed clots prepared from plasma with added purified plasminogen. The plasminogen was recovered in the washing fluid. According to these tests, then, purified added plasminogen is washed out of the clots.The plasminogen content of 20 thrombi obtained post mortem was also determined. The mean value was found to be 0.7 ACU/cm thrombus. Judging from our results, the “intrinsic clot lysis theory” is not the main mechanism of clot dissolution.

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