STUDIES ON A RECEPTOR ASSAY FOR AN ANTIBODY TO HUMAN THYROID PLASMA MEMBRANE

1979 ◽  
Vol 91 (1) ◽  
pp. 89-98 ◽  
Author(s):  
Yukio Ochi ◽  
Shiro Hosoda ◽  
Takashi Hachiya ◽  
Manabu Yoshimura ◽  
Tadayoshi Miyazaki ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Human Thyroid ◽  
Hashimoto’S Disease ◽  
Receptor Assay ◽  
Microsomal Antibody ◽  
Hashimoto's Disease ◽  
Hyperthyroid Patients ◽  
Test Serum ◽  
Serum Tsh

ABSTRACT A receptor assay was examined for the thyroid stimulating immunoglobulin using [125I]LATS. The principle of this method is the determination of displaced [125I]LATS from thyroidal plasma membrane (PM) by test serum. LATS-IgG without thyroidal antibodies was labelled with 125I, and radioactivity (RA) bound to purified PM from human thyroid was used as [125I] LATS. In the routine receptor assay, human thyroidal PM fraction, 0.1 ml of test serum, and [125I]LATS were incubated. Then [125I]LATS bound with the PM was determined after centrifugation. LATS positive serum showed a significant displacement. However, TSH (human and bovine), hCG and cholera toxin did not displace [125I]LATS from the plasma membrane. The bound per cent in euthyroid subjects was about 29 ± 3 % (mean ± sd). The displacing activity was positive in both hyperthyroid patients and in Hashimoto's thyroiditis, while in simple goitre and in hypothyroid patients with increased serum TSH it was always negative. LATS positive sera showed strong displacing activity in 16 of 17 cases, but LATS negative sera in hyperthyroid patients were positive in only 70 %. The displacing activity was also positive in all sera with positive precipitating antibody for thyroglobulin (TG), while patients with the positive haemagglutination antibody for TG (TRC: ≦ 107)in Hashimoto's disease were positive in about 50 % of cases. The displacing activity was not affected by absorption of TG antibody. Microsomal antibody in Hashimoto's disease was suggested to have an effect on the displacing activity. Although this method is not specific for determining LATS, this assay may be useful for examining thyroid plasma membrane binding immunoglobulin.

AN INVESTIGATION OF THE PATHOGENESIS OF HASHIMOTO'S DISEASE BY THYROID TISSUE CULTURE

1960 ◽  
Vol 20 (2) ◽  
pp. 83-NP ◽  
Author(s):  
W. J. IRVINE
Keyword(s):  
Tissue Culture ◽  
Alternative Hypothesis ◽  
Thyroid Tissue ◽  
Human Thyroid ◽  
Rabbit Serum ◽  
Thyroid Cell ◽  
Thyroid Extract ◽  
Thyroid Cells ◽  
Hashimoto’S Disease ◽  
Hashimoto's Disease

SUMMARY Human thyroid cells were grown in tissue culture in media containing normal human serum, Hashimoto serum, and rabbit sera containing antibodies to purified human thyroglobulin and to crude thyroid extract, respectively. The thyroid cells grew equally well in all media, with the exception of the rabbit serum containing antibodies to crude thyroid extract. Intact thyroid cells obtained from tissue culture failed to fix Hashimoto antibodies in the presence of complement, whereas the constituents of disrupted thyroid cells gave a strongly positive complement-fixation test with Hashimoto serum. It is therefore suggested that the intact thyroid cell is impermeable to complement-fixing Hashimoto antibody. The evidence afforded by the present work adds further weight to the belief that Hashimoto's disease may not be due to a simple auto-immunizing process consequent upon the interaction of thyroid antigen and the known circulating auto-antibodies. Evidence in support of an alternative hypothesis involving 'cell-bound' antibodies with disruption of the follicular basement membrane is discussed.

ULTRACENTRIFUGAL PATTERNS OF THE THYROID PROTEINS FROM HUMAN PATHOLOGICAL THYROID GLANDS

1969 ◽  
Vol 62 (4) ◽  
pp. 577-586 ◽  
Author(s):  
John A. Thomson ◽  
Joyce M. Bissett
Keyword(s):  
Anaplastic Carcinoma ◽  
Human Thyroid ◽  
Hashimoto’S Disease ◽  
Thyroid Glands ◽  
Normal Human ◽  
Hashimoto's Disease

ABSTRACT The ultracentrifugal pattern of the thyroid proteins from seven 'normal' human thyroid glands, 24 thyrotoxic glands, 28 cases of non-toxic, two cases of Hashimoto's disease, and three of anaplastic carcinoma of the thyroid have been examined. It has been demonstrated in thyrotoxicosis as compared to non-toxic goitre that there is a failure of formation of proteins > 19S. It is suggested that in the thyrotoxic gland there may be some defect in the manufacture of protein > 19S. In Hashimoto's disease an increased quantity of 6S protein was present in both cases studied. An increased quantity of lightweight protein was also found in cases of anaplastic carcinoma.

Retrospective revaluation of the significance of thyroid microsomal antibody in the treatment of Graves' disease

1987 ◽  
Vol 114 (3) ◽  
pp. 328-335 ◽  
Author(s):  
Noboru Hamada ◽  
Kunihiko Ito ◽  
Takashi Mimura ◽  
Naofumi Ishikawa ◽  
Naoko Momotani ◽  
...  
Keyword(s):  
Antithyroid Drug ◽  
Poor Response ◽  
Graves Disease ◽  
Radiation Doses ◽  
Retrospective Revaluation ◽  
Hashimoto’S Disease ◽  
Results Of Treatment ◽  
Microsomal Antibody ◽  
Hashimoto's Disease ◽  
Antithyroid Drug Treatment

Abstract. The results of treatment were analyzed in relation to serum microsomal antibody (MCAb) titre before treatment in 1185 patients with Graves' disease. The percentage of patients who had ablative therapy because of poor response to antithyroid drug treatment was significantly greater in those with MCAb haemagglutination test (MCHA) titres greater than 1:25 000. With 131I treatment, the patients with MCHA titres greater than 1:6400 responded significantly less to therapy, although the analysis was done in 146 selected patients with certain defined radiation doses and small goitres. With surgical treatment, the percentage of the patients entering into remission was significantly smaller for patients with MCHA titres greater than 1:25 000, because of an increase in both hypothyroidism and relapses. The incidence of hypothyroidism was significantly higher in patients with marked lymphocyte infiltration and/or lymphoid follicles. The degree of these histological findings in Graves' disease was not marked in spite of high MCAb titre and it was significantly different from that in Hashimoto's disease when analyzed in relation to the MCHA titre. These data indicate that in Graves' patients with high MCAb titre, remission is difficult to obtain by treatment, and suggest that the significance of MCAb is different in Graves' disease and Hashimoto's disease. The titre in Graves' disease may be one expression of the activity of this disease.

SERUM TRIIODOTHYRONINE DETERMINATION BY SATURATION ANALYSIS

1973 ◽  
Vol 72 (4) ◽  
pp. 714-726 ◽  
Author(s):  
A. Burger ◽  
B. Miller ◽  
C. Sakoloff ◽  
M. B. Vallotton
Keyword(s):  
Ionic Strength ◽  
Limit Of Detection ◽  
Improved Method ◽  
Accuracy Of Measurement ◽  
Tracer Amount ◽  
The Mean ◽  
Physiological Values ◽  
Hyperthyroid Patients ◽  
Solvent Blank

ABSTRACT An improved method for the determination of serum triiodothyronine (T3) has been developed. After addition of a tracer amount of the hormone, T3 was extracted from 1 ml serum under conditions of pH and ionic strength which favoured T3 extraction (89%) over thyroxine (T4) extraction (58%). Chromatography of the extracted material on Sephadex LH-20 separated T3 completely from residual T4. The T3 eluate was dried, then re-dissolved in 0.5 ml NaOH 0.04 n. To 0.2 ml duplicate aliquots, a standard amount of TBG was added for the competitive protein analysis. After one hour incubation at 4°C, separation of bound from free T3 was achieved on small Sephadex G-25 columns. Overall recovery was 67 ± 10.8% and correction for the loss was made. The solvent blank was 37 ± 27 (sd) ng/100 ml. Accuracy of measurement of known quantities of T3 added to serum was 98.4%. The coefficient of variation within the assay was 6.2% and between the assays it was 11.4%. The limit of detection (0.1 ng) corresponded to a concentration of 25 ng/100 ml. T4 added to serum did not interfere with T3 determination until high non-physiological values were reached. The mean ± sd serum T3 in 54 euthyroid subjects was 153 ± 58 ng/100 ml and in 24 hyperthyroid patients it was 428 ±186 ng/100 ml; 4 out of the 24 hyperthyroid values were within 2 sd of the mean euthyroid group. All the values found in the euthyroid group were well above the limit of detection of the method.

The assessment of vitamin D3 deficiency in patients with Hashimoto's disease and the relationship between the disease duration and 25OHD3 levels

2018 ◽  
Author(s):  
Katarzyna Lizis-Kolus ◽  
Alicja Hubalewska-Dydejczyk ◽  
Anna Sowa-Staszczak ◽  
Anna Skalniak ◽  
Aldona Kowalska ◽  
...  
Keyword(s):  
Vitamin D3 ◽  
Disease Duration ◽  
Hashimoto’S Disease ◽  
Hashimoto's Disease ◽  
The Relationship ◽  
Vitamin D3 Deficiency
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