NSILA AND FOETAL GROWTH

1979 ◽  
Vol 90 (3) ◽  
pp. 534-543 ◽  
Author(s):  
U. E. Heinrich ◽  
D. S. Schalch ◽  
M. H. Jawadi ◽  
C. J. Johnson
Keyword(s):  
Gestational Age ◽  
Serum Growth Hormone ◽  
Premature Newborns ◽  
Foetal Growth ◽  
Protein Binding Assay ◽  
Competitive Protein Binding Assay ◽  
Term Newborns ◽  
Competitive Protein Binding ◽  
Cortisol Levels ◽  
Serum Gh

ABSTRACT Employing a sensitive competitive protein binding assay for NSILA (non-suppressible insulin-like activity), circulating levels of this somatomedin (SM) have been measured throughout pregnancy, at parturition, and in foetal and newborn sera. Acid-dissociable serum NSILA (mean ± sem) in 57 women was significantly higher during pregnancy (1106 ± 46 μU/ml), than in 11 adult non-pregnant control subjects (844 ± 22 μU/ml), but not correlated with week of gestation or with serum growth hormone (GH) or cortisol levels. At parturition, the NSILA concentration in 28 cord sera (598 ± 38 μU/ml) was significantly less than in the corresponding maternal sera (1039 ± 63 μU/ml). The NSILA levels in 23 premature newborns (370 ± 20 μU/ml) and 8 smallfor-gestational-age newborns (310 ± 46 μU/ml) were significantly less than in 33 term newborns (494 ± 18 μU/ml). Serum NSILA in 56 term and premature newborns exhibited a significant positive correlation both with gestational age and birth weight but not with serum GH or cortisol levels. These data suggest that the maternal-foetal growth-promoting system is a highly complex one in which NSILA levels both in maternal and foetal circulations appear to be under multifactorial control.

RELIABILITY CRITERIA FOR SATURATION ANALYSIS OF STEROIDS BY COMPETITIVE PROTEIN BINDING

1970 ◽  
Vol 65 (1_Suppl) ◽  
pp. S61-S78 ◽  
Author(s):  
Billy D. Reeves ◽  
David W. Calhoun
Keyword(s):  
Protein Binding ◽  
Assay Method ◽  
Statistical Control ◽  
Protein Binding Assay ◽  
Competitive Protein Binding Assay ◽  
System 2 ◽  
Competitive Protein Binding ◽  
Single Method ◽  
Assay Design ◽  
Proper Perspective

ABSTRACT This communication is an attempt to delineate and define reliability criteria for saturation analysis of steroids by competitive protein binding assay. The discussion of these criteria evolved from three major considerations of assay method that help to place the ultimate criterion of accuracy in proper perspective. These major considerations are: 1) the measurement system, 2) the assay design and 3) the calculations and statistical control. Such an approach permits an evaluation, both relative and absolute, for a single method or for multiple methods.

TESTOSTERONE AND DIHYDROTESTOSTERONE CONCENTRATIONS IN THE FLUID MILIEU OF SPERMATOZOA IN THE REPRODUCTIVE TRACT OF THE BULL

1973 ◽  
Vol 74 (1) ◽  
pp. 186-200 ◽  
Author(s):  
Venkataseshu K. Ganjam ◽  
Rupert P. Amann
Keyword(s):  
Mass Spectrometry ◽  
Seminal Plasma ◽  
Reproductive Tract ◽  
Binding Assay ◽  
Rete Testis ◽  
Protein Binding Assay ◽  
Highly Sensitive ◽  
Competitive Protein Binding Assay ◽  
Competitive Protein Binding ◽  
Isolated Compound

ABSTRACT Total 17β-hydroxyandrogen concentrations were determined using a competitive protein binding assay, for bovine reproductive fluids. Rete testis fluid and cauda epididymal plasma were separated from the spermcontaining fluids obtained through cannulae from conscious bulls. Al-through the concentration of total 17β-hydroxyandrogens in rete testis fluid was similar (P > 0.05) to that in cauda epididymal plasma (25 and 19 ng/ml), both fluids contained higher (P < 0.01) androgen concentrations than seminal plasma, accessory sex gland fluid or serum from peripheral blood (3–5 ng/ml). However, since the amount of cauda epididymal plasma recovered was much less than for rete testis fluid (0.25 vs 35 ml/day), cauda epididymal plasma contained less than 1 % of the total 17β-hydroxyandrogens which entered the epididymis in rete testis fluid (5 vs 883 ng/day). Testosterone and/or dihydrotestosterone were isolated from the reproductive fluids by Sephadex LH-20 chromatography and quantified by a simple, specific and highly sensitive microassay. Dihydrotestosterone was found only in cauda epididymal plasma (14 ng/ml); identification of the isolated compound was confirmed by mass spectrometry. Dihydrotestosterone accounted for 52% of the 17β-hydroxyandrogens in cauda epididymal plasma while 23 % was testosterone. Testosterone represented 70 % of the 17β-hydroxyandrogens in rete testis fluid and 91 % of those in blood serum. Physiological implications of this shift in androgen balance are discussed.

A Simplified competitive protein-binding assay for 25-hydroxycalciferol

1976 ◽  
Vol 68 (2) ◽  
pp. 99-105 ◽  
Author(s):  
B. Garcia-Pascual ◽  
A. Peytremann ◽  
B. Courvoisier ◽  
D.E.M. Lawson
Keyword(s):  
Protein Binding ◽  
Binding Assay ◽  
Protein Binding Assay ◽  
Competitive Protein Binding Assay ◽  
Competitive Protein Binding

A Competitive Protein-Binding Assay for 25-Hydroxycholecalciferol

1975 ◽  
Vol 17 (2) ◽  
pp. 57-57
Author(s):  
Yoshiki Seino ◽  
Tsunesuke Shimotsuji ◽  
Shintaro Okada ◽  
Teisuke Hiejima ◽  
Chiiko Ikehara ◽  
...  
Keyword(s):  
Protein Binding ◽  
Binding Assay ◽  
Protein Binding Assay ◽  
Competitive Protein Binding Assay ◽  
Competitive Protein Binding ◽  
25 Hydroxycholecalciferol

Seasonal variation in plasma LH, FSH, prolactin, and testosterone concentrations in adult male white-tailed deer

1978 ◽  
Vol 56 (1) ◽  
pp. 121-127 ◽  
Author(s):  
R. E. Mirarchi ◽  
B. E. Howland ◽  
P. F. Scanlon ◽  
R. L. Kirkpatrick ◽  
L. M. Sanford
Keyword(s):  
Binding Assay ◽  
Follicle Stimulating Hormone ◽  
Late Winter ◽  
Protein Binding Assay ◽  
Antler Growth ◽  
Competitive Protein Binding Assay ◽  
Competitive Protein Binding ◽  
The Relationship ◽  
Wild Deer ◽  
Androgen Levels

Blood samples were collected monthly from captive and wild adult (12 months old or older) male white-tailed deer (Odocoileus virginianus) over 1 year in southwest Virginia. Plasma was assayed for luteinizing hormone (LH), follicle-stimulating hormone (FSH), prolactin (PRL), and testosterone (T) using radioimmunoassays. LH and T levels for the captive and wild deer were essentially similar and followed a distinct annual cycle. LH concentrations (nanograms per millilitre) peaked earlier (October, captive, 4.5 ± 1.8 (mean ± standard error); September, wild, 3.3 ± 0.9) than T concentrations (nanograms per millilitre) (November, captive, 13.3 ± 2.7; November, wild, 23.7 ± 7.8) and dropped off sharply prior to, or concomitant with, T concentrations. LH and T levels were lowest during the late winter and spring. T concentrations were closely correlated with androgen levels (Mirarchi, R. E., P. F. Scanlon, R. L. Kirkpatrick, and C. B. Schreck. J. Wildl. Manage. 41: 178–183 (1977)) determined by competitive protein binding assay. Mean PRL and FSH concentrations in wild and captive deer also displayed seasonal variations. Prolactin concentrations (nanograms per millilitre) were highest in May (147.5 ± 0.0) and lowest in November while FSH levels (nanograms per millilitre) peaked in September (180.2 ± 22.4) and were lowest in March. Differences in hormone concentrations between deer and sheep, and the relationship between PRL and antler growth and FSH and spermatozoan production, are discussed.

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