SERUM LEVELS OF TSH, T3, rT3, T4 AND T3-RESIN UPTAKE IN SURGICAL TRAUMA

1978 ◽  
Vol 88 (3) ◽  
pp. 482-489 ◽  
Author(s):  
H.-O. Adami ◽  
H. Johansson ◽  
L. Thorén ◽  
L. Wide ◽  
G. Åkerström
Keyword(s):  
Reference Range ◽  
Binding Capacity ◽  
Serum Levels ◽  
Suppressive Effect ◽  
Surgical Trauma ◽  
Hormone Metabolism ◽  
Thyroid Hormone Metabolism ◽  
Endogenous Cortisol ◽  
Protein Binding Capacity

ABSTRACT Changes in thyroid hormone metabolism induced by surgical trauma were studied by determination of TSH, T3, rT3, T4 and T3-resin uptake before, during and after mastectomy in 20 women and cholecystectomy in 10 women. Pronounced and reciprocal changes, explained by altered peripheral T4 metabolism were found concerning T3 and rT3. The decreased T3 and increased rT3-levels approached or exceeded the limits of the reference range on the first post-operative day. A significant increase in T3-resin uptake and a decrease in serum T4 were already observed during surgery and probably was caused by a decreased protein binding capacity. An increased concentration of TSH in the serum during surgery was followed by a significant post-operative decrease, possibly due to a suppressive effect of endogenous cortisol. No obvious difference in absolute values or the pattern of change was found between the mastectomy and cholecystectomy groups, although a somewhat more pronounced and retarded alteration in rT3 was found after cholecystectomy.

scholarly journals Effect of Micronutrients on Thyroid Parameters

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Hari Krishnan Krishnamurthy ◽  
Swarnkumar Reddy ◽  
Vasanth Jayaraman ◽  
Karthik Krishna ◽  
Qi Song ◽  
...  
Keyword(s):  
Amino Acids ◽  
Significant Positive Correlation ◽  
Clinical Laboratory ◽  
Serum Levels ◽  
Strong Positive Correlation ◽  
Healthy Individuals ◽  
Hormone Metabolism ◽  
Positive Correlation ◽  
Thyroid Hormone Metabolism ◽  
Iron And Zinc

Micronutrients are involved in various vital cellular metabolic processes including thyroid hormone metabolism. This study aimed to investigate the correlation between serum levels of micronutrients and their effects on thyroid parameters. The correlation of serum levels of micronutrients and thyroid markers was studied in a group of 387 healthy individuals tested for thyroid markers (T4, T3, FT4, FT3, TSH, anti-TPO, RT3, and anti-Tg) and their micronutrient profile at Vibrant America Clinical Laboratory. The subjects were rationalized into three groups (deficient, normal, or excess levels of micronutrients), and the levels of their thyroid markers were compared. According to our results, deficiency of vitamin B2, B12, and B9 and Vit-D25[OH] ( p < 0.05 ) significantly affected thyroid functioning. Other elemental micronutrients such as calcium, copper, choline, iron, and zinc ( p < 0.05 ) have a significant correlation with serum levels of free T3. Amino acids asparagine (r = 0.1765, p < 0.001 ) and serine (r = 0.1186, p < 0.05 ) were found to have a strong positive correlation with TSH. Valine, leucine, and arginine ( p < 0.05 ) also exhibited a significant positive correlation with serum levels of T4 and FT4. No other significant correlations were observed with other micronutrients. Our study suggests strong evidence for the association of the levels of micronutrients with thyroid markers with a special note on the effect of serum levels of certain amino acids.

Homeorhesis during early lactation. Euthyroid sick-like syndrome in lactating cows

1985 ◽  
Vol 110 (4) ◽  
pp. 505-509 ◽  
Author(s):  
C. Aceves ◽  
A. Ruiz-J. ◽  
C. Romero ◽  
C. Valverde-R.
Keyword(s):  
Environmental Temperature ◽  
Weather Conditions ◽  
Serum Levels ◽  
First Trimester ◽  
Early Lactation ◽  
Hormone Metabolism ◽  
Thyroid Hormone Metabolism ◽  
Clear Cut ◽  
Lactating Cows ◽  
Different Seasons

Abstract. Serum levels of thyroxine (T4), triiodothyronine (T3) and reverse T3 (rT3) were studied in dry, early (first trimester) and middle (second trimester) lactating purebred Holstein cows. The study encompassed three different seasons: autumn, winter and spring. Under comfortable weather conditions (temperature 22°C; relative humidity, 40%) or moderately hot (28°C; 60%), January and October, respectively, cows in early lactation exhibited significantly lower levels of T4 and T3, and higher values of rT3 than dry or middle lactating animals. In contrast, during May, when environmental temperature increased (34°C; 40%), a clear-cut shift in T3/rT3ratio occurred, and animals in early lactation exhibited the highest T3 and the lowest rT3 concentrations. These findings suggest that in dairy cattle, peripheral thyroid hormone metabolism plays a major role in regulating the homeorhetic responses involved in the maintenance of high priority functions.

scholarly journals A sensitive and rapid HPLC-DAD method for the determination of 3-hydroxy-1,2-dimethyl-4-pyridone and its distribution in rats

2020 ◽  
Vol 19 (4) ◽  
pp. 859-864
Author(s):  
Mengdi Chen ◽  
Xuejiao Zhang ◽  
Shuling Li ◽  
Qiongyao Zhang
Keyword(s):  
Protein Binding ◽  
Tissue Distribution ◽  
Plasma Protein Binding ◽  
Plasma Protein ◽  
Binding Capacity ◽  
Peak Level ◽  
Hplc Method ◽  
Protein Binding Capacity

Purpose: To establish a sensitive and rapid method for the determination of the tissue distribution of 3-hydroxy-1,2-dimethyl-4-pyridone (L1) in vivo, and its plasma protein binding capacity.Methods: This study optimized a reverse-phase HPLC method for specific and sensitive determination of L1 as well as its plasma and tissue  distributions. The optimized method was used to determine the plasma protein-binding capacity of L1 in Wistar rats.Results: A rapid, sensitive and simple HPLC-DAD method was established for studying the plasma and tissue distribution of L1. Following TI  administration, its liver concentrations peaked at 60 min and 360min, followed 360 min later with peak level in the kidney (second highest). The L1 concentration was significantly lower after 360 min than after 60 min, and values of its mean binding to plasma proteins was 5.2 % at different L1 concentrations.Conclusion: These results indicate that L1 is a drug with rapid-absorption and rapid-elimination thath is distributed widely in vivo in rats. Moreover, the drug has a weak plasma protein-binding capacity. Keywords: 3-Hydroxy-1,2-dimethyl-4-pyridone, Distribution, Alzheimer’s disease, Therapy

scholarly journals Determination of Cut-off Serum Values for Resistin and S100B Protein in Patients Who Survived a Cardiac Arrest

2019 ◽  
Vol 5 (3) ◽  
pp. 97-102
Author(s):  
Raluca M. Tat ◽  
Adela Golea ◽  
Ştefan C. Vesa ◽  
Daniela Ionescu
Keyword(s):  
Cardiac Arrest ◽  
Reference Range ◽  
Serum Levels ◽  
Range Limits ◽  
S100b Protein ◽  
Time Interval ◽  
Healthy Controls ◽  
Serum Concentrations ◽  
Median Serum

Abstract Introduction In an attempt to identify patients who have successfully survived a resuscitated cardiac arrest (CA), attention is drawn to resistin and S100B protein, two biomarkers that have been studied in relation to CA. Aim The study aimed to identify the potential cut-off serum values for resistin and S100B in patients who had CA, compared to healthy volunteers, given that, currently, none of the markers have normal and pathological reference range limits for human assay levels related to this pathology. Materials and Methods Forty patients, resuscitated after out-of-hospital CA and forty healthy controls, were included in the study. All patients were followed up for seventy-two hours after CA or until death. Blood samples for biomarkers were collected on admission to the ED (0-time interval) and at 6, 12, 24, 48 and 72 hours following resuscitation. Only one blood sample was collected from the controls. The serum concentrations of biomarkers were measured. Results For each time interval, median serum levels of resistin and S100 B were ​ significantly higher in patients with CA compared to healthy controls. The cut-of value for resistin in patients with CA, at the 12-hours versus controls, was > 8.2 ng/ml. The cut-of value for S100B in patients with CA versus controls recorded at 6 hours, was > 11.6 pg/ml. Conclusion Serum levels of resistin and S100B are higher among resuscitated CA patients compared to controls.

scholarly journals THE THYROID UNDER THREAT IN A WORLD OF PLASTICS

2018 ◽  
pp. 47
Author(s):  
Leonidas H. Duntas
Keyword(s):  
Thyroid Hormone ◽  
Thyroid Dysfunction ◽  
Suppressive Effect ◽  
Hormone Metabolism ◽  
Academic Organizations ◽  
Call To Action ◽  
Thyroid Hormone Metabolism ◽  
Organ Systems ◽  
Aqueous Environments ◽  
Major Organ

Among the various categories of thyroid disruptors, plasticizers, particularly phthalates and bisphenol A and substitutes, are most frequently examined due to their very extensive use and extreme durability. Both experimental and clinical studies have shown the deleterious effects of plasticizers on, among other major organ systems, thyroid physiology and thyroid hormone metabolism. Though the mechanism(s) are not as yet well clarified, it is hypothesized that plasticizers exert a suppressive effect on thyroid function and disrupt thyroid signaling. Similar effects have been reported in wildlife, which is also increasingly exposed to the plastic contamination of both solid and aqueous environments. By presenting the results of several recently published large studies linking plastics to thyroid dysfunction, this review aims to sound the alarm and thus call to action all academic organizations in order to counteract a threat which imperils not only the thyroid and the reproductive system but also the entirety of life on our planet.

Human Prothrombin Activation: Determination of Plasma and Serum Levels of Prothrombin Fragment 3 by Radioimmunoassay

1979 ◽  
Author(s):  
Daniel Walz ◽  
Thomas Brown
Keyword(s):  
Blood Clotting ◽  
Factor Xa ◽  
Heart Association ◽  
Serum Levels ◽  
Cross Reactivity ◽  
Assay Procedure ◽  
A Chain ◽  
Prothrombin Activation ◽  
Theoretical Amount

Human prothrombin activation is unique in that, in addition to the release of fragment 1.2 (FI.2) from the NH-terminus of prothrombin by factor Xa during the generation of thrombin, an additional 13 residue polypeptide, fragment 3 (F3), is autocatalytically removed from the amino-terminus of the thrombin A chain. We have developed a rapid radioimmunoassay for human F3 which incorporates short incubation times and the use of a preprecipitated second antibody; the assay can be performed in three hours. Specificity studies in buffer systems show prothrombin and prethrombin 1 cross-reacting at a level of 0.001; purified thrombin does not cross-react. In the presence of 5% BSA, prothrombin displays considerably less cross-reactivity. No immunoreactive material to F3 antibodies could be detected in 400 μL of plasma. Serum, obtained from whole blood clotting, contained measurable quantities of F3 (40-100 ng/mL). This amount in serum represents only 5-10% of the theoretical amount available should all of the fragment be hydrolytically cleaved during the conversion of prothrombin to thrombin. This assay procedure is currently being utilized to monitor the activation of purified human prothrombin in the absence and presence of selected plasma inhibitors. (Supported in part by NIH 05384-17 and the Michigan Heart Association).

scholarly journals Circulating bioactive sclerostin levels in an Austrian population-based cohort

2021 ◽  
Author(s):  
Katharina Kerschan-Schindl ◽  
Ursula Föger-Samwald ◽  
Andreas Gleiss ◽  
Stefan Kudlacek ◽  
Jacqueline Wallwitz ◽  
...  
Keyword(s):  
Serum Levels ◽  
Population Based ◽  
Negative Regulator ◽  
Total Serum ◽  
Mineral Density ◽  
Cross Sectional ◽  
Robust Parameter ◽  
Austrian Population ◽  
Different Levels

Summary Background Circulating serum sclerostin levels are supposed to give a good estimation of the levels of this negative regulator of bone mass within bone. Most studies evaluating total serum sclerostin found different levels in males compared to females and in older compared to younger subjects. Besides an ELISA detecting total sclerostin an ELISA determining bioactive sclerostin has been developed. The aim of this study was to investigate serum levels of bioactive sclerostin in an Austrian population-based cohort. Methods We conducted a cross-sectional observational study in 235 healthy subjects. Using the bioactive ELISA assay (Biomedica) bioactive sclerostin levels were evaluated. Results Serum levels of bioactive sclerostin were higher in men than in women (24%). The levels correlated positively with age (r = 0.47). A positive correlation could also be detected with body mass index and bone mineral density. Conclusion Using the ELISA detecting bioactive sclerostin our results are consistent with data in the literature obtained by different sclerostin assays. The determination of sclerostin concentrations in peripheral blood thus appears to be a robust parameter of bone metabolism.

SUBSTITUTION OF RAPESEED MEAL FOR SOYBEAN MEAL AS A SOURCE OF PROTEIN FOR LACTATING COWS

1976 ◽  
Vol 56 (2) ◽  
pp. 233-242 ◽  
Author(s):  
L. J. FISHER ◽  
DOROTHY S. WALSH
Keyword(s):  
Milk Yield ◽  
Dry Matter ◽  
Rapeseed Meal ◽  
Milk Composition ◽  
Hormone Metabolism ◽  
Thyroid Hormone Metabolism ◽  
Lactating Cows ◽  
Lower Blood ◽  
Concentrate Mixture ◽  
Low Erucic Acid

Sixteen lactating Holstein cows were randomly assigned to treatment sequences in a 4 × 4 changeover experiment. Experimental periods were 28 days in length separated by 7 days for changeover. Cows were fed corn silage free choice and gradually increasing amounts of one of four dairy concentrates containing 0, 11, 22, or 34% rapeseed meal (RSM) of a Canadian, experimental, low glucosinolate–low erucic acid variety. Concentrate intake (kg dry matter/day), milk yield (kg/day) and body weight change (kg/28 days) were 8.45a, 8.71a, 8.37a and 7.17b; 24.40a, 24.03ab, 22.92bc and 21.96c and 7.6b, 22.4b, 13.0ab, and 3.0b for 0, 11, 22 and 34% RSM mixtures, respectively. There was no influence of treatment on milk composition. Ration dry matter (DM) and nitrogen digestibilities declined (P < 0.05) as the RSM in the concentrate mixture increased. The amount of nitrogen excreted in the urine was greatest for cows fed 0% RSM and least for cows fed 34% RSM. Cows fed concentrate mixtures containing 34% RSM had a lower blood thyroxine level than others (P < 0.05), but RSM-containing concentrates had no apparent effect on the conversion of thyroxine to triiodothyronine. Results of the trial indicated that this experimental variety of RSM had no detrimental effect on concentrate intake, DM digestibility, milk composition or thyroid hormone metabolism when included at levels up to 22% in the concentrate mixture. However, milk yield and apparent digestibility of ration nitrogen were decreased (P < 0.05) by the 22% RSM mixture. It was not certain from the current findings whether the depression in digestibility was due largely to the high oil content of the meal or due to the higher fiber content of the RSM-supplemented rations.

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