HUMAN PROSTATIC CARCINOMA: SIGNIFICANT DIFFERENCES IN ITS ANDROGEN BINDING AND METABOLISM COMPARED TO THE HUMAN BENIGN PROSTATIC HYPERTROPHY

1978 ◽  
Vol 88 (2) ◽  
pp. 397-407 ◽  
Author(s):  
M. Krieg ◽  
I. Grobe ◽  
K. D. Voigt ◽  
E. Altenähr ◽  
H. Klosterhalfen
Keyword(s):  
Benign Prostatic Hypertrophy ◽  
Prostatic Hypertrophy ◽  
Sex Hormone Binding Globulin ◽  
Receptor Sites ◽  
Receptor Concentration ◽  
Group A ◽  
The Difference ◽  
Group B ◽  
Layer Chromatography

ABSTRACT The in vitro binding of 5α-dihydrotestosterone (5α-DHT), oestradiol-17β (Oe2) and methyltrienolone (R1881) was quantified in the 100 000 × g cytosol of 14 human prostatic carcinomas (PCA) by agargel electrophoresis, and compared, in part, to respective data obtained from human benign prostatic hypertrophy (BHP) samples. Furthermore, the in vitro metabolism of added 5α-DHT, testosterone (T) and 5α-androstane-3α,17β-diol in 10 of the 14 PCA, and for comparison, in 16 BPH samples, was analyzed by thin-layer chromatography, after a 30 min incubation period at 37°C. The main results were as follows: 1) Besides a sex hormone-binding globulin (SHBG) binding peak, in each of the 14 PCA cytosols a [3H]5α-DHT receptor binding could be demonstrated. By competition studies with unlabelled 5α-DHT a mean 5α-DHT receptor concentration of 30.9 fmol/mg cytosol protein (CP) (range 6.0–93.5) was calculated. In each of 7 cytosolic aliquots, incubated parallely with Oe2, an Oe2-binding to the receptor could be demonstrated, the concentration amounting to 8.4 fmol/mg CP (range 4.2–14.3). Simultaneous receptor quantification with R1881 in 4 cases revealed results, which are identical with the respective 5α-DHT data. 2) Dividing the PCA samples in group A (n = 6), consisting exclusively of adenocarcinomas, and group B (n = 8), consisting predominantly of a cribriform or cribriform and low differentiated tumour type, group B has a significantly (P < 0.05) higher assayable receptor concentration than group A. Comparing the total PCA with the BPH group, the former group has significantly (P < 0.04) higher levels of 5α-DHT receptor sites. 3) Comparing the mean cytosolic SHBG concentration of the PCA (93.2 fmol/mg CP) with the BPH (39.9 fmol/mg CP), the difference is significant (P < 0.01). Furthermore with respect to the PCA, there exists a significantly (P < 0.02) positive correlation between the plasmatic and cytosolic SHBG concentration. 4) The metabolic studies revealed that much more of the added T or 5α-DHT remains unmetabolized in the PCA than in the BPH. After T incubation, the amount of identified 5α-DHT plus 5α-androstanediols is on average 15 % lower in the PCA compared to the BPH. If 5α-DHT is added, the amount of identified 5α-androstanediols is on average 47 % lower in the PCA than in the BPH.

IN VITRO STUDIES OF TESTOSTERONE AND 5α-DIHYDROTESTOSTERONE BINDING IN BENIGN PROSTATIC HYPERTROPHY

1974 ◽  
Vol 75 (4) ◽  
pp. 773-784 ◽  
Author(s):  
P. Steins ◽  
M. Krieg ◽  
H. J. Hollmann ◽  
K. D. Voigt
Keyword(s):  
High Speed ◽  
Biological Fluids ◽  
Benign Prostatic Hypertrophy ◽  
Prostatic Hypertrophy ◽  
Sex Hormone Binding Globulin ◽  
List Type ◽  
Plasma Samples ◽  
Experimental Conditions ◽  
Displacement Experiments

ABSTRACT The cytosol fractions of prostatic adenoma and of the rectus abdominis muscle, together with the respective plasma samples were investigated in 13 patients in order to clarify whether or not there is an androgen receptor in human benign prostatic hypertrophy. The following methods were used: High speed ultracentrifugation in a sucrose gradient and agar gel electrophoresis at low temperature, of the 100 000 g supernatants after in vitro incubation with 3H-testosterone, 3H-5α-dihydrotestosterone, and 3H-oestradiol-17β. In parallel experiments the supernatants were heated to 45°C for 1 h before the steroid incubation. Displacement experiments with a 100–500-fold excess of various cold androgens. In the supernatants as well as in the plasma samples the total protein concentration was measured by the biuret reaction. The concentration of albumin and of immunoglobulin G (IgG) in the various biological fluids was determined by quantitative immuno diffusion. After charcoal stripping the binding of testosterone and of 5α-dihydrotestosterone was estimated quantitatively and correlated to the plasma contamination. By the methods used no physico-chemical differences in the androgen binding properties of plasma and of prostate cytosol were observed. However, in any individual cytosol a higher 5α-DHT binding than could be related to the plasma contamination was obtained. The question therefore remains open, as to whether the augmented 5α-dihydrotestosterone binding relates to a cellular increase of sex hormone binding globulin (SHBG) or to androgen receptors which do not show up clearly under the experimental conditions used.

scholarly journals Influence of Irrigation with NaOCl and Chlorhexidine on Microleakage

2015 ◽  
Vol 19 (1) ◽  
pp. 38-42
Author(s):  
Zoumpoulia Mylona ◽  
Christos Gogos ◽  
Nikolaos Economides
Keyword(s):  
Fisher Exact Test ◽  
Human Teeth ◽  
Fluid Filtration ◽  
Filtration Method ◽  
Exact Test ◽  
Group A ◽  
The Difference ◽  
Group B ◽  
Endodontic Sealers

SUMMARYBackground: Irrigation during endodontic therapy is required in order to remove debris, tissue remnants, microbes and smear layer. Sodium hypochlorite (NaOCl) and Chlorhexidine (CHX) are the most commonly used irrigants. Although they are reported to have good antimicrobial effects, both have limitations. Hence, a combination of NaOCl and CHX has been proposed to compensate for these limitations. However, this association forms a dense, orange-brown precipitate that stains walls of the pulp chamber. The aim of this study was to clarify in vitro if this precipitate affects the microleakage of endodontic sealers.Material and Methods: Extracted human teeth were used for this study. The teeth were cut at the height of the cervix and instrumented with NiTi rotary instruments. They were then divided into 2 experimental groups. In the first group (Group A) irrigations were performed with 2ml NaOCl 1%, 1ml EDTA 17% and 1ml CHX 0.2% and in the second (Group B) with 2ml NaOCl and 2ml CHX. Following this, they were obturated with gutta-percha and roth sealer. The microleakage was determined using a fluid filtration method. The measurements were repeated a month later. All analyses were performed using Fisher exact test.Results: Microleakage of Group A was lower than microleakage of Group B but the difference was not statistically significant.Conclusion: The precipitate that is formed by NaOCl and CHX did not affect microleakage of endodontic sealers.

scholarly journals Analysis of the difference in characteristics of patients and IVF cycles and the parameters of in vitro embryo culture between normal individuals with high and low blastocyst formation rate

2020 ◽  
Author(s):  
Chun-mei Yu ◽  
Xiu-liang Dai ◽  
yu-feng Wang ◽  
Li Chen
Keyword(s):  
Embryo Culture ◽  
Formation Rate ◽  
Ovarian Response ◽  
Individual Characteristics ◽  
Blastocyst Formation ◽  
Significant Difference ◽  
Group A ◽  
The Difference ◽  
Group B

Abstract Background: The main purpose of this study is to analyze the possible factors that were related with high useful blastocyst formatio rate in normal patients.Methods: This was a retrospective cohort study included 706 normal patients(excluding PCOS, DOR, endometriosis and other special diseases),according to the useful blastocyst in vitro formation rate(UBIVFR), the patients were divided into two groups respectively,group A（0-50%,including 50%）and group B（50%-100%). Results: There were no significant difference in patient ages between the two groups,compared with group A , the level of basic LH(bLH) was significantly increased. The clincal data showed that ,compared with group A ,the total Gn dose and the number of dominant follicles , oocytes retrieved，MII mature oocytes, normal fertilized oocytes rate,high-quality embryos rate and FET clinical pregnancy rate were significantly higher than group B.Conclusion:Our study is to investigate the useful blastocyst formation rate in relation to bLH levels in IVF/ICSI cycles, showing that high bLH is associated with higher ovarian response and higher UBIVFR.AMH,bFSH and AFC which were defined as ovarian reserve and ovarian response are seem not associated with the useful blastocyst formation. Key words:Useful blastocyst formation; normal patients; individual characteristics; basic LH; ovarian response; in vitro embryo culture

QUANTIFICATION OF ANDROGEN BINDING, ANDROGEN TISSUE LEVELS, AND SEX HORMONE-BINDING GLOBULIN IN PROSTATE, MUSCLE AND PLASMA OF PATIENTS WITH BENIGN PROSTATIC HYPERTROPHY

1977 ◽  
Vol 86 (1) ◽  
pp. 200-215 ◽  
Author(s):  
M. Krieg ◽  
W. Bartsch ◽  
S. Herzer ◽  
H. Becker ◽  
K. D. Voigt
Keyword(s):  
Binding Capacity ◽  
Benign Prostatic Hypertrophy ◽  
Average Concentration ◽  
Sex Hormone ◽  
Prostatic Hypertrophy ◽  
Sex Hormone Binding Globulin ◽  
Receptor Protein ◽  
Hormone Binding ◽  
Tissue Levels

ABSTRACT The in vitro binding of 5α-dihydrotestosterone (5α-DHT) in benign prostatic hypertrophy (BPH), rectus abdominis muscle and plasma of 14 patients was characterized and quantified by agargel electrophoresis. The respective endogenous tissue and plasma levels of 5α-DHT and testosterone (T) were determined by radioimmunoassay, and the plasmatic sex hormone-binding globulin (SHBG) concentration was estimated in the 14 patients by an (NH4)2SO4 precipitation technique. Finally the in vitro conversion of 5α-DHT to the 5α-androstanediols in the BPH at 0°C after a 20–24 h incubation period was analyzed by thin-layer chromatography. The main results were as follows: (1) In 12 out of 14 BPH cytosols three charcoal resistant binding peaks were found, of which peak 1 represents SHBG, peak 2 the specific receptor protein and peak 3 a binding protein with relatively high binding capacity and low affinity for 5α-DHT. In two cases peak 2 was absent. In 11 out of 14 muscle cytosols three binding peaks are also present, resembling those of the BPH. (2) The receptor peak is reduced on average 38 % by unlabelled 5α-DHT, 23 % by cyproterone acetate (CYAC) and 29 % by oestradiol. The parallel data for the SHBG peak are: 62% by 5α-DHT, 22% by CYAC and 49% by oestradiol. (3) From displacement studies with unlabelled 5α-DHT the average concentration of receptor was calculated to be 12.3 fmol/mg cytosol protein (CP) in BPH, and 3.6 fmol/mg CP in muscle. Under identical conditions 39.9 fmol SHBG/mg CP and 24.1 fmol/mg CP were found in the BPH and muscle, respectively. The mean values are significantly different (P < 0.001). In plasma a mean value of 4.0 × 10−8 mol SHBG/1 was found. (4) In the BPH on average 4.43 ng 5α-DHT/g tissue and 0.23 ng T/g tissue are present, in muscle 0.45 ng 5α-DHT/g tissue and 0.71 ng T/g tissue, in plasma 0.47 ng 5α-DHT/ml and 3.89 ngT/ml. (5) Statistical calculations revealed (a) a significantly (P < 0.05) negative correlation between the endogenous 5α-DHT and T tissue levels and the available 5α-DHT receptor sites in BPH cytosol, (b) a positive correlation between plasmatic SHBG concentration and the available SHBG concentration in BPH cytosol. (6) Compared to the rat prostate, where 36 % of the incubated 5α-DHT was converted at 0°C within 20–24 h into the 5α-androstanediols, in the BPH conversion to 5α-androstanediols was negligible.

Factor II Related Antigen and Antithrombin III Levels as Indicators of Liver Failure in Consumption Coagulopathy

1982 ◽  
Vol 47 (03) ◽  
pp. 218-220 ◽  
Author(s):  
P Sié ◽  
E Letrenne ◽  
C Caranobe ◽  
M Genestal ◽  
B Cathala ◽  
...  
Keyword(s):  
Liver Failure ◽  
Antithrombin Iii ◽  
Coagulation Factors ◽  
Consumption Coagulopathy ◽  
Blood Coagulation Factors ◽  
Factor Ii ◽  
At Iii ◽  
Group A ◽  
Group B

SummaryIn order to detect impaired synthesis of blood coagulation factors associated to consumption coagulopathy, a simultaneous evaluation of factor II-related antigen (II rAg) and of antithrombin III (AT III) was carried out in 16 patients affected with severe defibrination. An in vitro preliminary study on plasma and serum demonstrated that the levels of II rAg and of AT III, assessed by the Laurell technique with Behring antisera, were not reduced by the coagulation process. The patients were, a posteriori, classified into two groups according to the absence (group A) or the presence (group B) of factors predisposing to liver failure such as metastasis, cirrhosis, and prolonged shock. II rAg and AT III levels are significantly correlated; they are in the normal range in group A but reduced in group B. Thus II rAg or AT III level determinations are useful markers in the detection of liver failure associated to the consumption phenomenon. These results also suggest that part of the decreased AT III levels reported in severe cases of disseminated intravascular coagulation may be the consequence of an associated liver failure.

Efficacy and Safety of Oral Ivermectin and Topical Permethrin in the Treatment of Scabies

2020 ◽  
Vol 33 (1) ◽  
pp. 41-47
Author(s):  
Mohsena Akhter ◽  
Ishrat Bhuiyan ◽  
Zulfiqer Hossain Khan ◽  
Mahfuza Akhter ◽  
Gulam Kazem Ali Ahmad ◽  
...  
Keyword(s):  
Skin Diseases ◽  
Close Contact ◽  
Randomized Study ◽  
Sarcoptes Scabiei ◽  
Efficacy And Safety ◽  
Common Skin ◽  
Group A ◽  
The Mean ◽  
The Difference ◽  
Group B

Background: Scabies is one of the most common skin diseases in our country. It is caused by the mite Sarcoptes scabiei var hominis, which is an ecto-parasite infesting the epidermis. Scabies is highly contagious. Prevalence is high in congested or densely populated areas. Individuals with close contact with an affected person should be treated with scabicidal which is available in both oral and topical formulations. The only oral but highly effective scabicidal known to date is Ivermectin. Amongst topical preparations, Permethrin 5 % cream is the treatment of choice. Objective: To evaluate the efficacy & safety of oral Ivermectin compared to topical Permethrin in the treatment of scabies. Methodology: This prospective, non-randomized study was conducted at the out-patient department of Dermatology and Venereology of Shaheed Suhrawardy Medical College & Hospital over a period of 6 months, from August 2016 to January 2017. The study population consisted of one hundred patients having scabies, enrolled according to inclusion criteria. They were divided into two groups. group A was subjected to oral Ivermectin and the group B to Permethrin 5% cream. Patients were followed up on day 7 and 14 for assessment of efficacy and safety. Result: The mean scoring with SD in group A (Ivermectin) and group B (Permethrin) were 8.26 ± 2.22 and 7.59 ± 2.01 respectively at the time of observation. The difference between the mean score of the two group is not significant (p=0.117) the mean scoring with SD in group A and group B were 4.54 ± 2.05 and 1.64 ± 1.84 respectively at 7thdays. The difference between the mean score of the two group is significant (p<0.001). The mean scoring with SD in group A and group B were 2.68± 2.35 and .36± 1.10 respectively at 14th day difference between the mean score of the group is significant (p<0.001). Conclusion: Topical application of permethrin 5% cream is more effective and safer than oral Ivermectin in the treatment of scabies. TAJ 2020; 33(1): 41-47

Effect of Sample Storage Conditions on Body Burden Analysis of Organic Contaminants in Unionid Mussels

1992 ◽  
Vol 27 (4) ◽  
pp. 833-844 ◽  
Author(s):  
Micheline Hanna
Keyword(s):  
Organic Contaminants ◽  
Body Burden ◽  
Storage Conditions ◽  
The Body ◽  
Sample Storage ◽  
Pcb Congeners ◽  
Unionid Mussels ◽  
Group A ◽  
The Difference ◽  
Group B

Abstract In order to quantitatively assess the effect of sample storage conditions on the body burden analysis of organic contaminants, a comparative analysis was carried out on the unionid mussel Elliptic complanata. The mussels were divided into two groups, each with distinct storage conditions, while Group A was kept in the freezer at −20°C, Group B was kept in the refrigerator for five days at 5°C. All the compounds present in the control were also present in Group B samples. Analysis of the organic contaminants in each of these two groups showed that for total PCB concentrations, the two treatments were not significantly different; however when compared individually 6 of the 13 PCB congeners showed significant differences. The observed differences were relatively small for individual PCB congeners (7.1 to 15.3%), higher for chlorobenzenes (10.5 to 36.4%), and yet higher for HCE (44.1%); the difference for HCE, although large is nevertheless not significant, even if only marginally so.

scholarly journals The Pandemic and Changes in the Self-Perception of Teacher Digital Competences of Infant Grade Students: A Cross Sectional Study

2021 ◽  
Vol 18 (9) ◽  
pp. 4756
Author(s):  
Rosalía Romero-Tena ◽  
Carmen Llorente-Cejudo ◽  
María Puig-Gutiérrez ◽  
Raquel Barragán-Sánchez
Keyword(s):  
Intervention Group ◽  
Cross Sectional Study ◽  
Self Perception ◽  
Digital Competence ◽  
Cross Sectional ◽  
Self Perceptions ◽  
Group A ◽  
Different Dimensions ◽  
The Difference ◽  
Group B

Without having a reaction time, the pandemic has caused an unprecedented transformation in universities around the world, leading to a revolution from structured models anchored in the conception of transmission of training towards a teaching approach-learning saved thanks to the incorporation of technology. This study aims to verify whether the pandemic situation has influenced the digital competence self-perception of students. Comparing two groups during the academic years 2019/2020 and 2020/2021, the instrument used is the questionnaire for digital competence “DigCompEdu Check-In” for future teachers. After the educational intervention, group A (before COVID-19) presented higher self-perceptions of competence than group B (during COVID-19); the pandemic situation caused by COVID-19 has negatively influenced students’ self-perception of their digital skills in the pretest in the different dimensions under study. Before receiving the training, the group that did not experience the pandemic enjoyed a higher self-perception of their competencies than the group that experienced the pandemic. The data obtained indicate that the difference exists, and that it is statistically significant, and may be a consequence of the clear relationship between self-perception and the way in which students face reality through their personal and subjective vision.

scholarly journals The Impact of Left Atrial Size in Catheter Ablation of Atrial Fibrillation Using Remote Magnetic Navigation

2018 ◽  
Vol 2018 ◽  
pp. 1-8
Author(s):  
Xiao-yu Liu ◽  
Hai-feng Shi ◽  
Jie Zheng ◽  
Ku-lin Li ◽  
Xiao-xi Zhao ◽  
...  
Keyword(s):  
Atrial Fibrillation ◽  
Catheter Ablation ◽  
Left Atrial ◽  
Magnetic Navigation ◽  
Remote Magnetic Navigation ◽  
X Ray ◽  
Group A ◽  
Group B ◽  
The Impact

Objective. The objective of this study was to investigate the impact of left atrial (LA) size for the ablation of atrial fibrillation (AF) using remote magnetic navigation (RMN). Methods. A total of 165 patients with AF who underwent catheter ablation using RMN were included. The patients were divided into two groups based on LA diameter. Eighty-three patients had small LA (diameter <40 mm; Group A), and 82 patients had a large LA (diameter ≥40 mm; Group B). Results. During mapping and ablation, X-ray time (37.0 (99.0) s vs. 12 (30.1) s, P<0.001) and X-ray dose (1.4 (2.7) gy·cm2 vs. 0.7 (2.1) gy·cm2, P=0.013) were significantly higher in Group A. No serious complications occurred in any of the patients. There was no statistical difference in the rate of first anatomical attempt of pulmonary vein isolation between the two groups (71.1% vs. 57.3%, P=0.065). However, compared with Group B, the rate of sinus rhythm was higher (77.1% vs. 58.5%, P<0.001) during the follow-up period. More patients in Group A required a sheath adjustment (47/83 vs. 21/82, P<0.001), presumably due to less magnets positioned outside of the sheath. In vitro experiments with the RMN catheter demonstrated that only one magnet exposed created the sheath affects which influenced the flexibility of the catheter. Conclusions. AF ablation using RMN is safe and effective in both small and large LA patients. Patients with small LA may pose a greater difficulty when using RMN which may be attributed to the fewer magnets beyond the sheath. As a result, the exposure of radiation was increased. This study found that having at least two magnets of the catheter positioned outside of the sheath can ensure an appropriate flexibility of the catheter.

Does body position, age, and heart rate induce IOP’s changes?

2021 ◽  
pp. 112067212110233
Author(s):  
Marcelina Sobczak ◽  
Magdalena Asejczyk ◽  
Malwina Geniusz
Keyword(s):  
Heart Rate ◽  
Body Position ◽  
Age Groups ◽  
Cardiovascular Parameters ◽  
Entire Cohort ◽  
Significant Difference ◽  
Group A ◽  
The Difference ◽  
Group B ◽  
Effect Of Age

Objectives: The main goal of this research was to determine the differences between the values of intraocular pressure (IOP) in the supine and sitting positions, and to assess the effect of age and cardiovascular parameters. Methods: Seventy-two healthy adults were enrolled and classified into age groups: 20–30 years (group A), 31–40 years (group B), and 41–71 years (group C). Corneal biometry and cardiovascular parameters, such as heart rate (HR), were measured. IOP measurements were taken in the sitting position (IOPS) and in the supine position (IOPL) using the iCare® Pro tonometer. Results: A significant difference between the IOPS and IOPL in the entire cohort was found ( p < 0.001). Regarding the age subgroups, a significant difference ( p < 0.001) between the IOPS and IOPL was obtained in group A (2.6 ± 1.6 mmHg) and group C (1.5 ± 1.3 mmHg). There were no significant differences in the IOPS between groups. The highest IOP values were obtained for group A. The correlations between HR and IOPS are statistically significant for group A and group B, and for HR and IOPL-S for group B only. Multivariate analysis showed that HR has a significant influence on the difference in IOP in the two body positions. Conclusion: A statistically significant difference between the effect of age and the values of IOPS and IOPL was shown. Cardiovascular parameters showed some relevant statistical dependencies, but with a rather marginal significance in young people. The influence of body position for the measurement of IOP for healthy subjects does not seem to matter, despite the fact that there are some dependencies that are statistically significant.

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