RADIOIMMUNOASSAYS OF TETRAHYDROALDOSTERONE (TH-ALDO) IN HUMAN URINE

1978 ◽  
Vol 87 (3) ◽  
pp. 596-608 ◽  
Author(s):  
K.-H. Kohl ◽  
P. Vecsei ◽  
S. Abdelhamid
Keyword(s):  
Bovine Serum Albumin ◽  
Normal Values ◽  
Rapid Screening ◽  
Chromatographic System ◽  
Polar Material ◽  
Butanol Extract ◽  
Specific Antisera ◽  
Aldosterone Production ◽  
Rapid Screening Test ◽  
New Zealand Rabbits

ABSTRACT Specific antisera against tetrahydroaldosterone (TH-Aldo) were raised in two white New Zealand rabbits. 3α,5β-TH-aldo-20-oxime-bovine-serum albumin complex was used as antigen. The resulting titers were 1:18 000 and 1:16 000. Except tetrahydrocortisol (THF) (0.23%) and tetrahydro-18-hydroxy-11-dehydrocorticosterone (18-OH-THA) (3.2 %), all steroids and steroid metabolites gave negligible cross-reactions. Immunograms of the paper chromatograms made from the n-butanol-extract of the urines, as well as after β-glucuronidase treatment and dichlormethane extraction, were studied to further define the specificity of the antiserum. Antibody H1 (used in this study) reacted with aldosterone-18-gluc., a TH-aldosterone-glucuronide (probably the 21-glucuronide) and an unidentified less polar material. Two methods were developed: a) TH-Aldo-glucuronidc(s) estimation after ethylacetate pre-extraction as a rapid screening test of endogenous aldosterone production. b) estimation of TH-aldosterone using one chromatographic system. The results of method a) showed a significant correlation with the values obtained by technique b). Normal values (method b) were 25.88 ± 16.50 μg/24h (range 9.5–64.8 μg/24h). A significant correlation was also shown between the TH-aldo (technique b) and 18-gluc. values.

RADIOIMMUNOASSAY OF TETRAHYDROCORTICOSTERONE (THB) IN HUMAN URINE

1978 ◽  
Vol 88 (1) ◽  
pp. 139-148 ◽  
Author(s):  
K.-H. Kohl ◽  
K.-H. Gless ◽  
S. Abdelhamid ◽  
B. Penke ◽  
P. Vecsei
Keyword(s):  
New Zealand ◽  
Bovine Serum Albumin ◽  
Human Urine ◽  
Bovine Serum ◽  
Normal Values ◽  
Chromatographic System ◽  
Acth Stimulation ◽  
Cross Reactions ◽  
Dexamethasone Suppression ◽  
New Zealand Rabbits

ABSTRACT Antisera against tetrahydrocorticosterone (THB) (one of the metabolites of the glucocorticoid corticosterone) were raised in white New Zealand rabbits. A 3α,5β-THB-20-oxime-bovine serum albumin complex was used as antigen. The resulting titers were 1/300–1/19 000. The cross-reactions of the best antiserum were: THDOC 2.25%, THB-21-glucuronide 39%, and THF-21-glucuronide 4.8%. Other steroids and steroid metabolites have given negligible cross-reactions, e. g. allo-(3α,5α-)-THB 0.53 %, tetrahydro-11-dehydro-corticosterone (THA) 0.92%, allo-THA 0%, and THB-3-glucuronide 0 %. Two methods were introduced: a) for screening an estimation of THB-glucuronide, mostly THB-21-glucuronide ("THB-gluc."), in unprocessed urine after simple dilution b) specific estimation of THB using one paper chromatographic system. The values estimated by method a) showed a highly significant correlation (r = 0.9654, P < 0.01) with the values obtained by technique b). Normal values (method b) measured in subjects hospitalized under standardized conditions were: 21.8–177.6; mean 89.1 μg/24 h. THB levels and "THB-gluc." levels react to ACTH stimulation and dexamethasone suppression and thus we propose that the methods are useful for the diagnosis of hyper- and hypocorticosterone production states.

Rapid screening test for porphyrinuria

1970 ◽  
Vol 102 (2) ◽  
pp. 237-237
Author(s):  
R. M. McDonald
Keyword(s):  
Screening Test ◽  
Rapid Screening ◽  
Rapid Screening Test

A Rapid Screening Test for Detection of Poisons in Blood

Nature ◽  
1962 ◽  
Vol 193 (4822) ◽  
pp. 1298-1298 ◽  
Author(s):  
H. V. STREET
Keyword(s):  
Screening Test ◽  
Rapid Screening ◽  
Rapid Screening Test

Erythrocyte adenosine transport a rapid screening test for cardiovascular drugs

1993 ◽  
Vol 30 (3) ◽  
pp. 163-167 ◽  
Author(s):  
Pollen K.F. Yeung ◽  
Susan J. Mosher ◽  
Rongshi Li ◽  
Patrick S. Farmer ◽  
Gerald A. Klassen ◽  
...  
Keyword(s):  
Screening Test ◽  
Cardiovascular Drugs ◽  
Rapid Screening ◽  
Rapid Screening Test ◽  
Adenosine Transport

scholarly journals On the effects of some carcinogenic hydrocarbons on the growth of sporelings of marine red algae

1962 ◽  
Vol 42 (3) ◽  
pp. 579-585 ◽  
Author(s):  
A. D. Boney ◽  
E. D. S. Corner
Keyword(s):  
Aromatic Hydrocarbons ◽  
Red Algae ◽  
Considerable Increase ◽  
Rapid Screening ◽  
Cell Production ◽  
Low Concentrations ◽  
Rapid Screening Test ◽  
Polycyclic Aromatic ◽  
Carcinogenic Hydrocarbons ◽  
Derivatives Of

Low concentrations of various carcinogenic polycyclic aromatic hydrocarbons cause a considerable increase in cell production when applied to sporelings of certain marine red algae.Whereas low concentrations of carcinogenic derivatives of benzanthracene stimulate cell production, similar concentrations of structurally related non-carcinogens inhibit it.The applicability of the method as a rapid screening test for carcinogens is discussed.

Rapid Solubility Test for Detection of Hemoglobin S

1971 ◽  
Vol 17 (11) ◽  
pp. 1081-1082 ◽  
Author(s):  
Jean E Matusik ◽  
James B Powell ◽  
David M Gregory
Keyword(s):  
Phosphate Buffer ◽  
Screening Test ◽  
Rapid Screening ◽  
Hemoglobin S ◽  
Solubility Test ◽  
Negative Results ◽  
Rapid Screening Test

Abstract We have devised a rapid screening test for use in detecting sickling hemoglobins. This test depends on the insolubility of sickling hemoglobins in 2.24 molar phosphate buffer. Sickling hemoglobins form a turbid suspension in this test, in which positive and negative results, respectively, appear very much like those for the "Sickledex" test.

Rapid screening test evaluation for microalbuminuria in diabetes mellitus

1998 ◽  
Vol 35 (4) ◽  
pp. 199-202 ◽  
Author(s):  
I. Fernández Fernández ◽  
J. M. Páez Pinto ◽  
T. Hermosín Bono ◽  
P. Vázquez Garijo ◽  
M. A. Ortiz Camuñez ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Screening Test ◽  
Rapid Screening ◽  
Test Evaluation ◽  
Rapid Screening Test
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