DECREASE OF NON-SUPPRESSIBLE INSULIN-LIKE ACTIVITY AFTER PANCREATECTOMY AND NORMALIZATION BY INSULIN THERAPY

1977 ◽  
Vol 85 (4) ◽  
pp. 818-822 ◽  
Author(s):  
J. E. Eigenmann ◽  
M. Becker ◽  
B. Kammermann ◽  
W. Leemann ◽  
R. Heimann ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Acetic Acid ◽  
Insulin Therapy ◽  
Serum Samples ◽  
Hormone Levels ◽  
Severe Diabetes ◽  
Before And After ◽  
Insulin Like Activity ◽  
Parallel Growth

ABSTRACT Non-suppressible insulin-like activity (NSILA-S) was determined in 5 dogs before and after pancreatectomy and again during insulin therapy. All NSILA-S determinations were carried out on serum samples which were passed over Sephadex G-50 columns equilibrated with 1 m acetic acid. The levels of NSILA-S decreased drastically shortly after pancreatectomy and rose slowly after institution of insulin therapy, to normal levels. During the period of severe diabetes after pancreatectomy the concentration of growth hormone was elevated. These findings indicate that 1) the pancreas cannot be the site of synthesis and release of NSILA-S, 2) NSILA-S levels do not always parallel growth hormone levels and 3) the synthesis and secretion of NSILA-S among other factors is under the control of insulin.

THE INFLUENCE OF HYPOPHYSECTOMY ON NSILA CONCENTRATIONS IN THE DOG: EVIDENCE FOR PARTIALLY PITUITARY-INDEPENDENT REGULATION

1977 ◽  
Vol 86 (3) ◽  
pp. 498-503 ◽  
Author(s):  
J. E. Eigenmann ◽  
M. Becker ◽  
B. Kammermann ◽  
J. Zapf ◽  
W. Leemann ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Thyroid Hormone ◽  
Pituitary Gland ◽  
Normal Level ◽  
Binding Assay ◽  
Serum Samples ◽  
Protein Binding Assay ◽  
Before And After ◽  
Low Levels ◽  
Insulin Like Activity

ABSTRACT Non-suppressible insulin-like activity (NSILA) was determined in 5 dogs before and after hypophysectomy. All NSILA determinations were carried out on serum samples after acidic Sephadex G-50 chromatography by two different assay systems, i. e. a bioassay and a protein binding assay. The levels of NSILA decreased significantly after hypophysectomy and returned to near normal levels after 2 weeks. T3−, T4− and cortisol levels were drastically reduced during the entire period of the experiment. Several GH determinations after hypophysectomy revealed very low levels. Insulin-induced hypoglycaemia failed to provoke a rise of GH levels as late as 4 months after hypophysectomy. These findings indicate that: 1) The pituitary gland cannot be the site of synthesis of NSILA. 2) NSILA concentrations in the dog are maintained at a near normal level in the presence of very low growth hormone and thyroid hormone concentrations, so that these latter hormones do not appear to be the only regulatory factors concerned in NSILA synthesis.

PROLACTIN AND THE PITUITARY-GONADAL AXIS IN MALE URAEMIC PATIENTS ON REGULAR DIALYSIS

1976 ◽  
Vol 82 (1) ◽  
pp. 29-38 ◽  
Author(s):  
C. Hagen ◽  
K. Ølgaard ◽  
A. S. McNeilly ◽  
R. Fisher
Keyword(s):  
Growth Hormone ◽  
Follicle Stimulating Hormone ◽  
Plasma Concentrations ◽  
Sex Hormone Binding Globulin ◽  
Elevated Plasma ◽  
Hormone Binding ◽  
Hormone Levels ◽  
Significant Difference ◽  
Before And After ◽  
Male Patients

ABSTRACT In 21 consecutive adult male patients with chronic renal failure on regular haemo- or peritoneal dialysis, the plasma levels of prolactin (hPr), growth hormone (HGH), thyrotrophin (TSH), luteinizing hormone (LH), follicle stimulating hormone (FSH), testosterone (T) and sex hormone binding globulin (SHBG) were measured. Elevated levels of hPr were found in 16 of the patients and could not only be explained by the medicamentation. All the patients studied showed an inverse ratio of LH to FSH with higher levels of FSH than LH and 15 of the 21 patients had elevated plasma concentrations of FSH, while only 4 had elevated LH. No significant difference in any of the hormone levels could be demonstrated before and after dialysis, and no significant correlation between the hormone levels and the time of dialysis, the type of dialysis or the age of the patient was found. However, 8 of the 21 patients showed higher levels of HGH before than after dialysis. Impotency was found in 11 of the patients, but was not related to abnormal levels of hPr, LH, FSH, T or SHBG.

scholarly journals Percent reduction of growth hormone levels correlates closely with percent resected tumor volume in acromegaly

2015 ◽  
Vol 122 (4) ◽  
pp. 798-802 ◽  
Author(s):  
Lucia Schwyzer ◽  
Robert M. Starke ◽  
John A. Jane ◽  
Edward H. Oldfield
Keyword(s):  
Growth Hormone ◽  
Tumor Volume ◽  
Tumor Resection ◽  
Volume Growth ◽  
Residual Tumor ◽  
Close Correlation ◽  
Tumor Removal ◽  
Hormone Levels ◽  
Before And After ◽  
Plasma Gh

OBJECT Correlation between tumor volume and hormone levels in individual patients would permit calculation of the fraction of tumor removed by surgery, by measuring postoperative hormone levels. The goals of this study were to examine the relationship between tumor volume, growth hormone (GH), and insulin-like growth factor–1 (IGF-1) levels, and to assess the correlation between percent tumor removal and the reduction in plasma GH and IGF-1 in patients with acromegaly. METHODS The 3D region of interest–based volumetric method was used to measure tumor volume via MRI before and after surgery in 11 patients with GH-secreting adenomas. The volume of residual tumor as a fraction of preoperative tumor volume was correlated with GH levels before and after surgery. Examination of this potential correlation required selection of patients with acromegaly who 1) had incomplete tumor removal, 2) had precise measurements of initial and residual tumor, and 3) were not on medical therapy. RESULTS Densely granulated tumors produced more peripheral GH per mass of tumor than sparsely granulated tumors (p = 0.04). There was a correlation between GH and IGF-1 levels (p = 0.001). Although there was no close correlation between tumor size and peripheral GH levels, after normalizing each tumor to its own plasma GH level and tumor volume, a comparison of percent tumor resection with percent drop in plasma GH yielded a high correlation coefficient (p = 0.006). CONCLUSIONS Densely granulated somatotropinomas produce more GH per mass of tumor than do sparsely granulated tumors. Each GH-secreting tumor has its own intrinsic level of GH production per mass of tumor, which is homogeneous over the tumor mass, and which varies greatly between tumors. In most patients the fraction of a GH-secreting tumor removed by surgery can be accurately estimated by simply comparing plasma GH levels after surgery to those before surgery.

The Effect of Diphenylhydantoin on Insulin Response

1974 ◽  
Vol 46 (1) ◽  
pp. 131-136
Author(s):  
A. G. Cudworth ◽  
J. L. Cunningham
Keyword(s):  
Growth Hormone ◽  
Oral Dose ◽  
Glucose Tolerance ◽  
Serum Concentration ◽  
Insulin Response ◽  
Immunoreactive Insulin ◽  
Oral Glucose ◽  
Hormone Levels ◽  
Before And After ◽  
Different Doses

1. Serum immunoreactive insulin, glucose tolerance and growth hormone levels were estimated in healthy volunteers before and after receiving diphenylhydantoin sodium (DPH) for 14 days. 2. With a standard oral dose of DPH all subjects showed a reduced insulin response to oral glucose (11–44%). This correlated with the serum concentration of the drug, which ranged from 30.5 to 90.0 μmol/l (7.7–22.7 μg/ml). 3. Glucose tolerance remained normal and no changes in growth hormone levels were observed. 4. Different doses of DPH have been employed in the treatment of insulinoma and as a screening test for detecting latent insulin secretory defects. This study confirms the wide inter-individual variation in the metabolism of DPH, and stresses both the importance of measuring the serum concentration of DPH and the comparative irrelevance of dosage levels in these situations.

NON-SUPPRESSIBLE INSULIN-LIKE ACTIVITY AND SULPHATION ACTIVITY IN SERUM EXTRACTS OF NORMAL SUBJECTS, ACROMEGALICS AND PITUITARY DWARFS

1976 ◽  
Vol 81 (1) ◽  
pp. 28-42 ◽  
Author(s):  
U. Schlumpf ◽  
R. Heimann ◽  
J. Zapf ◽  
E. R. Froesch
Keyword(s):  
Molecular Weight ◽  
Growth Hormone ◽  
Acetic Acid ◽  
Large Scale ◽  
Normal Subjects ◽  
Step Procedure ◽  
Chromatographic Procedure ◽  
One Step ◽  
Insulin Like Activity

ABSTRACT Non-suppressible insulin-like activity (NSILA) is a term used for a variety of substances in serum, excluding insulin, which promote glucose uptake of adipose tissue and diaphragm in vitro. NSILA-S is a peptide with a molecular weight of 7000 which is soluble in acid ethanol and which has been purified on a large scale from human serum. This study describes a simple chromatographic one step procedure by which NSILA-S can be extracted and quantitatively measured in individual sera. Using Sephadex G-75 equilibrated with 1 m acetic acid, NSILA-S was detected only in one peak containing small molecular peptides. NSILA-S obtained with this one step chromatographic procedure exerted all the effects of purified NSILA-S including sulphation activity on the rat cartilage. All chromatographic fractions with NSILA-S also had sulphation activity. Both, NSILA-S and sulphation activity were increased in acromegalics and decreased in pituitary dwarfs. It is suggested that one molecule in serum is responsible for both activities which are, at least in part, under the control of growth hormone.

Serum free insulin-like growth factor-I in growth hormone-deficient adults before and after growth hormone replacement

1997 ◽  
pp. 132-137 ◽  
Author(s):  
C Skjaerbaek ◽  
N Vahl ◽  
J Frystyk ◽  
TB Hansen ◽  
JO Jorgensen ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Healthy Volunteers ◽  
Hormone Replacement ◽  
Hormone Deficiency ◽  
Molar Ratio ◽  
Serum Samples ◽  
Gh Treatment ◽  
Serum Free ◽  
Igf I ◽  
Before And After

The objective of the present study was to compare fasting levels of free IGF-I in serum from patients with adult onset growth hormone deficiency (GHD) and from healthy volunteers, and to examine the effect of GH replacement therapy in GHD on serum free IGF-I. Free IGF-I was measured using separation of free IGF-I by ultrafiltration in serum samples from 42 healthy volunteers and 27 patients with GHD, in the latter before and after 1 year of treatment with GH (2 IU/m2) (n = 13) or placebo (n = 14). Free IGF-I was significantly decreased in patients with GHD (700 +/- 100 ng/l (mean +/- S.E.M.), range 55-2618 ng/l) compared with controls (1010 +/- 70 ng/l, range 231-2431 ng/l; P = 0.0016). Total IGF-I was 85 +/- 10 micrograms/l (GHD) and 160 +/- 10 micrograms/l (controls) (P < 0.0001). The ratio of free over total IGF-I was increased in GHD to 0.85 +/- 0.08% compared with 0.66 +/- 0.05% in controls (P = 0.04). In both GHD and controls, free IGF-I correlated significantly (P < 0.05) with total IGF-I (GHD r = 0.78; controls r = 0.42), IGFBP-1 (GHD r = -0.67; controls r = -0.46) and the molar ratio of total IGF-I over IGFBP-3 (GHD r = 0.58; controls r = 0.62). After 1 year of GH treatment, free IGF-I was increased to 2780 +/- 320 ng/l (P = 0.003) and total IGF-I was increased to 270 +/- 30 micrograms/l (P = 0.006) both of which values were greater than those in healthy volunteers. There were no changes in free or total IGF-I in the placebo-treated group. In conclusion, levels of free IGF-I are decreased in GHD, but measurements of free IGF-I in a single, fasting serum sample do not offer a better separation of patients with GHD from individuals with normal GH status than can be achieved by measurement of total IGF-I. One year of treatment with 2IU/m2 GH caused an increase of serum free IGF-I to supraphysiological levels.

Study the Relationship Between Estrogen Hormone and Calcium in Normal Females Before and After Menopause in Baghdad / Iraq

2018 ◽  
Vol 2 (1) ◽  
Author(s):  
Sameerah Mustafa ◽  
Asal Tawfeeq ◽  
Hadeel Hasan
Keyword(s):  
Oral Contraceptive ◽  
Healthy Controls ◽  
Serum Samples ◽  
Oral Contraceptive Pills ◽  
Contraceptive Pills ◽  
Menopausal Women ◽  
Before And After ◽  
Group A ◽  
Group B ◽  
Age Range

This study involved the collection of (90) samples of women serum which included (30) serum samples collected from women before menopause (reproductive women) in the age range of (22-43) years and were considered as (group A- control). While, (group B) included (30) serum samples collected from women using oral contraceptive pills between the ages of (22-43) years old. Whereas, another (30) serum samples were collected from women after menopause between the ages of (43-54) years and were considered as (group C). All of the collected serum samples were subjected to a number of serological and chemical tests for the measurement of (E2, HDL, LDL and Ca). Then, the obtained data were statistical analyzed and results showed a significant decrease (p˂ 0.05) in (E2 ,Ca and HDL) levels in menopausal women compared to that of the normal healthy controls. While, there were non-significant decrease (p> 0.05) in (E2, Ca and HDL) levels in women taking oral contraceptive when compared to the normal healthy controls. On the other hand, a significant increase (p˂ 0.05) was recorded in LDL level in menopausal women compared to that of the normal healthy controls whereas, no-significant increase (p˃ 0.05) in the LDL level in women taking oral contraceptives when compared to the control women.

THE EFFECT OF THE PROGESTOGEN ETHYNODIOL DIACETATE ON GLUCOSE, INSULIN, AND GROWTH HORMONE AFTER SIX MONTHS TREATMENT

1972 ◽  
Vol 70 (2) ◽  
pp. 373-384 ◽  
Author(s):  
W. N. Spellacy ◽  
W. C. Buhi ◽  
S. A. Birk
Keyword(s):  
Growth Hormone ◽  
Blood Glucose ◽  
Plasma Insulin ◽  
Tolerance Test ◽  
Metabolic Effects ◽  
Oral Glucose ◽  
Hormone Levels ◽  
Glucose Levels ◽  
Ethynodiol Diacetate ◽  
Tolerance Curve

ABSTRACT Seventy-one women were treated with a daily dose of 0.25 mg of the progestogen ethynodiol diacetate. They were all tested with a three-hour oral glucose tolerance test before beginning the steroid and then again during the sixth month of use. Measurements were made of blood glucose and plasma insulin and growth hormone levels. There was a significant elevation of the blood glucose levels after steroid treatment as well as a deterioration in the tolerance curve in 12.9% of the women. The plasma insulin values were also elevated after drug treatment whereas the fasting ambulatory growth hormone levels did not significantly change. There was a significant association between the changes in glucose and insulin levels and the subject's age, control weight, or weight gain during treatment. The importance of considering the metabolic effects of the progestogen component of oral contraceptives is stressed.

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