SEX HORMONE BINDING GLOBULIN BINDING CAPACITY, TESTOSTERONE, 5α-DIHYDROTESTOSTERONE, OESTRADIOL AND PROLACTIN IN PLASMA OF PATIENTS WITH PROSTATIC CARCINOMA UNDER VARIOUS TYPES OF HORMONAL TREATMENT

1977 ◽  
Vol 85 (3) ◽  
pp. 650-664 ◽  
Author(s):  
W. Bartsch ◽  
H.-J. Horst ◽  
H. Becker ◽  
G. Nehse
Keyword(s):  
Cyproterone Acetate ◽  
Prostatic Carcinoma ◽  
Binding Capacity ◽  
Specific Binding ◽  
Hormonal Treatment ◽  
Sex Hormone ◽  
Sex Hormone Binding Globulin ◽  
Control Group ◽  
Blood Levels ◽  
Hormone Binding

ABSTRACT Sex hormone binding globulin (SHBG) binding capacity, the concentrations of testosterone (T), of 5α-dihydrotestosterone (DHT), of oestradiol-17β (Oe2), of oestrone (Oe1), of prolactin (hPr) and the percentual specific binding of T to SHBG (% TB) were measured in plasma of patients suffering from prostatic carcinoma and of a control group of similar age. No significant differences in any of the investigated parameters were found between the control group and the carcinoma patients before treatment although 15 % of the latter showed distinctly elevated hPr values. Treatment of carcinoma patients with 1) Antiandrogen (cyproterone acetate, Androcur®) resulted in a significant decrease of T, Oe2 and SHBG. The DHT/T-ratio increased. n = 5. 2) Orchidectomy caused an even more pronounced fall in T, DHT, Oe1 and Oe2 blood levels. SHBG was not altered. DHT/T-ratio increased. n = 32. 3) Cyproterone acetate after orchidectomy led to elevated hPr values. n = 5. 4) Oestrogen (diethylstilboestrol-diphosphate, Honvan®) after orchidectomy increased SHBG and hPr. n = 6. 5) Corticosteroid (Prednisone, Decortin®) after orchidectomy decreased T and SHBG below the levels found after orchidectomy alone, n = 5. 6) Diureticum (Mefruside, Baycaron®) (n = 5) or 7) a placebo (n = 7) did not alter any of the parameters measured. 8) Treatment with HCG (Primogonyl®) of patients suffering from oligozoospermia resulted in a significant increase of T, DHT and Oe2. SHBG was not altered. DHT/T-ratio decreased, n = 7.

PLASMA SEX HORMONE-BINDING GLOBULIN BINDING CAPACITY IN BENIGN PROSTATIC HYPERTROPHY AND PROSTATIC CARCINOMA: COMPARISON WITH AN AGE DEPENDENT RISE IN NORMAL HUMAN MALES

1977 ◽  
Vol 84 (1) ◽  
pp. 207-214 ◽  
Author(s):  
M. Dennis ◽  
H.-J. Horst ◽  
M. Krieg ◽  
K. D. Voigt
Keyword(s):  
Prostatic Carcinoma ◽  
Binding Capacity ◽  
Benign Prostatic Hypertrophy ◽  
Sex Hormone ◽  
Prostatic Hypertrophy ◽  
Sex Hormone Binding Globulin ◽  
Hormone Binding ◽  
Age Dependent ◽  
The Mean ◽  
Age Range

ABSTRACT A method for the routine determination of plasma sex hormone-binding globulin (SHBG) binding capacity is introduced. In normal males an age dependent increase in SHBG binding capacity from 2.85 × 10−8 m in the age range 22–44 years to 4.66 × 10−8 m in the age range 45–64 years was found. A higher mean value of 6.41 × 10−8 m was obtained in normal females, 20–40 years old, and still higher values were found in females taking oral contraceptives. Although in male groups with benign prostatic hypertrophy or prostatic carcinoma the mean ages were somewhat higher than in the older normal group, a further age dependent increase in SHBG binding capacity in these diseases could not be demonstrated. In fact the mean values found were slightly although not significantly lower at 4.07 and 3.89 × 10−8 m, respectively. As expected, oestrogen treatment of males with prostatic carcinoma produced higher values.

scholarly journals Plasma sex hormone binding globulin in patients with prostatic carcinoma

Cancer ◽  
1990 ◽  
Vol 66 (2) ◽  
pp. 354-357 ◽  
Author(s):  
Marco Grasso ◽  
Arturo Buonaguidi ◽  
Roberto Mondina ◽  
Giovanni Borsellino ◽  
Caterina Lania ◽  
...  
Keyword(s):  
Prostatic Carcinoma ◽  
Sex Hormone ◽  
Sex Hormone Binding Globulin ◽  
Hormone Binding

scholarly journals Modulation of SHBG binding to testosterone and estradiol by sex and morbid obesity

2017 ◽  
Vol 176 (4) ◽  
pp. 393-404 ◽  
Author(s):  
María del Mar Grasa ◽  
José Gulfo ◽  
Núria Camps ◽  
Rosa Alcalá ◽  
Laura Monserrat ◽  
...  
Keyword(s):  
Morbid Obesity ◽  
Binding Sites ◽  
Binding Capacity ◽  
Specific Binding ◽  
Sex Hormone Binding Globulin ◽  
Morbidly Obese ◽  
Hormone Binding ◽  
Anthropometric Measures ◽  
Obesity Status ◽  
And Gender

ObjectiveSex hormone-binding globulin (SHBG) binds and transports testosterone and estradiol in plasma. The possibility that SHBG is a mixture of transporting proteins has been postulated. We analyzed in parallel the effects of obesity status on the levels and binding capacity of circulating SHBG and their relationship with testosterone and estradiol.DesignAnthropometric measures and plasma were obtained from apparently healthy young (i.e. 35 ± 7 years) premenopausal women (n = 32) and men (n = 30), with normal weight and obesity (BMI >30 kg/m2).MethodsSHBG protein (Western blot), as well as the plasma levels of testosterone, estradiol, cortisol and insulin (ELISA) were measured. Specific binding of estradiol and testosterone to plasma SHBG was analyzed using tritium-labeled hormones.ResultsSignificant differences in SHBG were observed within the obesity status and gender, with discordant patterns of change in testosterone and estradiol. In men, testosterone occupied most of the binding sites. Estrogen binding was much lower in all subjects. Lower SHBG of morbidly obese (BMI >40 kg/m2) subjects affected testosterone but not estradiol. The ratio of binding sites to SHBG protein levels was constant for testosterone, but not for estradiol. The influence of gender was maximal in morbid obesity, with men showing the highest binding/SHBG ratios.ConclusionsThe results reported here are compatible with SHBG being a mixture of at least two functionally different hormone-binding globulins, being affected by obesity and gender and showing different structure, affinities for testosterone and estradiol and also different immunoreactivity.

Binding of Testosterone and Oestradiol to Sex Hormone Binding Globulin, Human Serum Albumin and other Plasma Proteins: Evidence for Non-Specific Binding of Oestradiol to Sex Hormone Binding Globulin

1983 ◽  
Vol 64 (3) ◽  
pp. 307-314 ◽  
Author(s):  
M. Jawed Iqbal ◽  
Maureen Dalton ◽  
Robert S. Sawers
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Binding Site ◽  
Plasma Proteins ◽  
Specific Binding ◽  
Sex Hormone ◽  
Sex Hormone Binding Globulin ◽  
Normal Female ◽  
Hormone Binding

1. The percentage binding of testosterone (T) and oestradiol (E2) to sex hormone binding globulin (SHBG) and human serum albumin (HSA) was determined over a range of SHBG concentrations of 16–250 nmol of dihydrotestosterone (DHT) bound/l. It was found that the binding of both T and E2 to HSA was a function of their binding to SHBG and bore an inverse relationship to it. After removal of both SHBG and HSA from plasma by affinity chromatography a ‘residual’ binding of about 11% for T and 12% for E2 was still apparent. in addition to the specific high-affinity, low capacity binding of E2 to SHBG, non-specific low-affinity binding of 7–12% was demonstrated after selective denaturation of the specific binding site of the latter. 2. Competition studies indicated that although at the relatively higher levels of SHBG found in the normal female the physiological concentrations of E2, T and DHT need not be taken into account in estimating the unbound fractions of steroids, at the relatively lower levels of SHBG found in normal men and hirsute women, the physiological concentrations of T and DHT are effective in causing statistically significant displacement of E2 from the common, specific binding site on SHBG. 3. A simple computerized technique is described for the determination of fractions of E2 and T respectively, that are unbound to SHBG, unbound to SHBG and HSA, and unbound to all plasma proteins, when the total plasma levels of E2, T, DHT and SHBG are known.

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