EFFECT OF AN OVULATORY DOSE OF LUTEINIZING HORMONE ON THE CONCENTRATION OF OESTRONE, OESTRADIOL AND PROGESTERONE IN THE RABBIT OVARIAN FOLLICLES

1976 ◽  
Vol 82 (4) ◽  
pp. 792-800 ◽  
Author(s):  
Vasant V. Patwardhan ◽  
André Lanthier
Keyword(s):  
Luteinizing Hormone ◽  
Follicular Fluid ◽  
Ovarian Follicles ◽  
Mature Female ◽  
The Other ◽  
Other Hand ◽  
High Concentrations ◽  
Sexually Mature

ABSTRACT This study was done to determine the effect of an ovulatory dose of LH on the concentration of oestrone, oestradiol and progesterone in the follicular tissue and in follicular fluid of ovaries of sexually mature female rabbits. Eight animals were sacrificed without treatment while others (4 to a group) were sacrificed at 1, 3, 4, 6, 8 and 10 h after administration of LH (50 μg). In each animal follicles from both ovaries were pooled and the follicular tissue was separated from the fluid. Determination of oestrone, oestradiol and progesterone was done by radioimmunoassay separately in the follicular tissue and in fluid. One hour after LH treatment oestrogen levels were found elevated, as compared to the control, in the fluid but not in the tissue. Thereafter oestrogen levels declined and reached levels much below control at times nearing ovulation. On the other hand, progesterone levels were elevated over the control in both the tissue and fluid at 1 and 3 h. The tissue progesterone levels were, however, below control at and after 6 h. The sustained high concentrations of tissue progesterone in the earlier period after LH stimulation could play a role in the chain of events leading to follicular rupture.

Effects of pregnant mare's serum gonadotrophin administered in vivo on steroid accumulation by isolated rabbit ovarian follicles

1984 ◽  
Vol 107 (4) ◽  
pp. 531-537 ◽  
Author(s):  
E. V. YoungLai
Keyword(s):  
Luteinizing Hormone ◽  
Follicular Fluid ◽  
Low Dose ◽  
Ovarian Follicles ◽  
Mature Female ◽  
Treatment Groups ◽  
Sexually Mature

Abstract. Various doses (5, 50 and 100 IU) of pregnant mare's serum gonadotrophin (PMS) were administered to sexually mature female rabbits and steroids measured in follicular fluid, ovarian pieces and in incubation media of isolated follicles treated with ovine luteinizing hormone (LH). Follicular fluid progesterone, oestradiol and androgen were increased after PMS injection. Ovarian progesterone content was increased by all doses of PMS, and androgen and oestradiol content only by 50 and 100 IU PMS. Medium sized follicles from rabbits treated with 50 and 100 IU PMS accumulated more progesterone than all follicles from rabbits treated with saline or 5 IU PMS. Androgen accumulation by small follicles from 50 and 100 IU dose rabbits was higher than that by follicles from other treatment groups. Oestradiol accumulation mimicked androgen accumulation. The addition of LH to the medium stimulated the accumulation of progesterone and androgen by all follicles. LH caused an increase in oestradiol accumulation by medium follicles from saline and low dose PMS treated animals. No effect of LH on oestrogen accumulation was seen with large or medium follicles from animals treated with 100 IU PMS or 50 IU PMS for 2 or 3 days. These results suggest that priming of small follicles with PMS can increase their ability to produce androgen which is probably aromatized to oestrogen.

scholarly journals Determination of the Ratios of Ligands to Metal Ion of some Metal Complexes of Triazoles by Using Eleetronie Speetra in Organie Solvents

2004 ◽  
Vol 1 (1) ◽  
pp. 110-115
Author(s):  
Baghdad Science Journal
Keyword(s):  
Solid State ◽  
Metal Complexes ◽  
Electronic Spectra ◽  
Metal Ion ◽  
The Other ◽  
Molar Ratio ◽  
Other Hand

We found that 4,5- diphenyl- 3(2- propynyl) thio- 1??-triazole [1? forms a complex with Pd (11) ion of ratio 1:1 which absorbs light in CH2CI2 at 400 nm, and 4,5- diphenyl- 3(2- propenyl) thio- 1,2,4- triazole [II] forms complexes with Pd (II) ion of ratio 1:1 which absorbs light at 390 nm, and of ratio 2:1 which absorbs light at 435 nm. On the other hand, we found that the new derivative 4- phenyl- 5( p- amino phenyl) -3- mercapto- 1,2,4- triazole ?111? forms complexes with Cu (II) ion of the ratio 1:1 which absorbs light at 380 nm, with Ni (II) ion of the ratio 3:1 which absorbs light at 358 nm; and with Co (11) ion of the ratio 3.2:1 which absorbs light at 588 nm. The ratio of the complexes were determined by measuring the electronic spectra of the complexes in CH2G2 and (CH^NCHO at different concentrations ofthe ligands and f?xed ' •' of the metal ion in every case, then applying the molar ratio plots on the data. Our results were confirmed by precipitating most ofthe above complexes in solid state, and then each complex was analyzed elementally.

scholarly journals Resin canals in spruce (Picea abis /L./ Karst.) with the occurrence of reaction wood

2005 ◽  
Vol 53 (1) ◽  
pp. 85-92 ◽  
Author(s):  
Vladimír Gryc ◽  
Petr Horáček
Keyword(s):  
Compression Wood ◽  
3D Models ◽  
Resin Canal ◽  
The Other ◽  
Stem Length ◽  
Reaction Wood ◽  
Spruce Wood ◽  
Other Hand ◽  
Resin Canals

The paper was aimed at the determination of variability of horizontal resin canal dimension in spruce wood in relation to the position in a spruce stem. Significant changes of dimensions in horizontal resin canal along the stem length and radius were found. On the basis obtained of results 3D models (for CW, OW, SWL and SWP zones) describing changes in resin canal dimensions in spruce in relation to the position in a stem were created. In the models, the resin canal dimension decreases with the height of a stem and on the other hand, with an increasing distance from the stem pith the dimension of resin canal increases. The importance of the paper consists in the enlargement of findings about the structure of spruce with compression wood.

Kvalitet pitanja u TV školi u nastavi prirode i društva

2021 ◽  
Author(s):  
Jelena Lukić ◽  
Keyword(s):  
Social Science ◽  
The Other ◽  
Educational Content ◽  
Educational Levels ◽  
School Year ◽  
Other Hand ◽  
Cognitive Levels ◽  
The Way

The aim of this paperis to determine the quality of TV classes World around us and Nature and Social Science,which were broadcasted on Radio Television of Serbia during the pandemic in the school year 2019/20. Although the work was indirect, so the immediate interaction between the teacher and student is missed. Therefore, teacher's questions were the way of establishing some kind of interaction in such classes organized in this manner. For this reason, we wanted to establish the types of questions that teachers were asking to students through small screens. Considering that the achievements of learning are based on Bloom's Taxonomy, we were analyzing sixteen TV classes and classified the questions the teachers asked according to cognitive area, on six educational levels. The results indicate that the most common were question within lower cognitive levels were (knowledge, understanding and application), and that there are no statistically significant differences in cognitive levels on questions asked between lower (1st and 2nd grade) and higher grades (3rd and 4th grade), on the other hand, on the classes of determination of educational content teachers were asking statistically significant quality questions compared to the classes of interpretation.

scholarly journals Markov chains and the determination of fair premiums

1967 ◽  
Vol 4 (3) ◽  
pp. 265-268 ◽  
Author(s):  
Stefan Vajda
Keyword(s):  
Stochastic Process ◽  
Markov Chains ◽  
Stochastic Processes ◽  
Mathematical Theory ◽  
The Other ◽  
Main Stream ◽  
Other Hand ◽  
Pure Mathematics ◽  
The University

The relationships between actuarial and pure mathematics are curious. Actuaries have contributed to the development of mathematical theory: it is sufficient to mention, as examples, Fredholm of an earlier, and Cramér of a more recent generation. Scandinavian mathematicians, in particular, have been concerned with a very special type of stochastic process, reflected in the collective theory of risk, and the work of Philipson, Ammeter and others in this field is well known to readers of this Bulletin. However, the main stream of the theory of stochastic processes has little contact with actuarial applications.On the other hand, many actuaries have studied and assimilated pure mathematics and have thrown light on actuarial matters by describing their own preoccupations in the terminology of modern, often abstract, mathematics. E. Franckx is one of their number.The Instituto di Matematica Finanziaria of the University of Trieste (Faculty of Economics and Commerce) has published a booklet entitledEssai d'une théorie opérationnelle des risques Markoviens which contains three lectures delivered by Professor Franckx in Trieste and a contribution which he presented to the 17th Congress of Actuaries, held in London in 1964.

Dermatoglyphics in Twins

1973 ◽  
Vol 22 (1) ◽  
pp. 91-98 ◽  
Author(s):  
Heloisa F. Peña ◽  
F.M. Salzano ◽  
Sidia M. Callegari
Keyword(s):  
Correlation Coefficients ◽  
The Other ◽  
Ridge Count ◽  
Genetic Determination ◽  
Qualitative Traits ◽  
Quantitative Measurements ◽  
Other Hand ◽  
Adult Twins

SummaryThe degree of genetic determination of 4 digital and 16 palmar characteristics was investigated in an unselected series of 49 MZ and 51 DZ adult twins. Low indications of heritability were obtained for the qualitative traits, but 6 of the 8 h2 estimates calculated for the quantitative measurements gave values above 0.70. In general, the results reported here are in agreement with those presented by other authors; however, the correlation coefficients observed for the A′d ridge count were lower than those of two other series. All palmar ridge counts seemed to be intercorrelated in a given individual; other expected relationships, however, were not observed. The fingerprints of MZ and DZ twins on the other hand, showed an unexplained difference.

Nutritional Requirement for 4-Aminobenzoate Caused by Mutation of Dihydropteroate Synthetase

1976 ◽  
Vol 31 (5-6) ◽  
pp. 285-287 ◽  
Author(s):  
Helmut Rappold ◽  
Adelbert Bacher
Keyword(s):  
Parent Strain ◽  
The Other ◽  
Synthetase Activity ◽  
Nutritional Requirement ◽  
Wild Type ◽  
Aerobacter Aerogenes ◽  
Low Level ◽  
Other Hand ◽  
High Concentrations

Abstract Aerobacter aerogenes mutant 62-1 AC requires high concentrations of 4-aminobenzoate for growth. The mutant accumulates N-glucosyl-4-aminobenzoate and has an intact 4-aminobenzoate synthetase (Bacher, Gilch, Rappold, and Lingens, Z. Naturforsch. 28c, 614 - 617 [1973]). On the other hand the ability of the mutant to synthesize dihydropteroate is markedly reduced. The dihydropteroate synthetase level of mutant 62-1 AC is 1% as compared to the parent strain. Spontaneous revertants of mutant 62-1 AC show wild type levels of dihydropteroate synthetase. We conclude that the requirement for 4-aminobenzoate in mutant 62-1 AC is due to poor utilization of 4-aminobenzoate as a consequence of the low level of dihydropteroate synthetase activity.

scholarly journals The effect of bread-making process on contents of key trichothecene mycotoxins: deoxynivalenol, T-2, and HT-2 toxins

2014 ◽  
Vol 32 (No. 6) ◽  
pp. 570-577 ◽  
Author(s):  
T. Dropa ◽  
J. Hajšlová ◽  
K. Lancová ◽  
I. Burešová
Keyword(s):  
Sample Preparation ◽  
Substantial Effect ◽  
The Other ◽  
Processing Conditions ◽  
Bread Making ◽  
Toxin Concentration ◽  
Other Hand ◽  
Contamination Levels ◽  
Wheat Flours

The fate of trichothecene mycotoxins deoxynivalenol (DON), T-2 and HT-2 toxins during the bread preparation and baking was investigated in order to obtain information about the influence of processing conditions on the levels of these toxins in final products. Highly artificially contaminated wheat was used to obtain flours with three contamination levels (DON 1615–398, T-2 toxin 927–160, and HT-2 toxin 258–38 μg/kg). Method for Test Baking of Wheat Flours (ICC Standard No.131) was used within the experiments for bread sample preparation. Various combinations of dough fermentation, proofing and baking times were used to prepare test breads. For determination of toxin levels in all tested matrices, HPLC–MS/MS method was employed. No substantial effect on DON levels was observed as a result of various conditions used for bread preparation and baking; maximum DON decrease 10%, compared to contaminated flour. On the other hand, significant changes in T-2/HT-2 toxin contents were found in experimental bread compared to contaminated flour; reduction of T-2 toxin concentration (30–50%) and increase of HT-2 toxin concentration (10–70%), respectively.  

Development of a Family Vocational Guidance Potential as a Core Prerequisite to Successful Professional Self-Determination of Teenagers

10.12737/3080 ◽  
2014 ◽  
Vol 2 (1) ◽  
pp. 55-61
Author(s):  
Зеленкина ◽  
Tatyana Zelenkina
Keyword(s):  
Professional Education ◽  
Vocational Guidance ◽  
The Other ◽  
Self Determination ◽  
Vocational Choice ◽  
Pilot Testing ◽  
Other Hand ◽  
New Concepts ◽  
Professional Self

Optimized way to nurture parents’ readiness to guide vocational choice of their teenaged children is considered as a consistent element of vocational guidance potential and is scientifically proved. What makes the paper theoretically important, is the fact that the author clarifies the meaning for quite new concepts, as «family vocational guidance potential», «parents’ helpfulness», «successful vocational choice». Families are classified in terms of vocational guidance potential; several limitations in vocational choices of children in each category of families are outlined and illustrated by pictures and tables. Given the accented challenge of insufficient parents’ integration in vocational choices, made by their children, the idea of pilot testing described here, has been to use capabilities of additional professional education establishments to better preparing parents to the mission of facilitating the vocational choices of their children. As a result of parents-children cooperation, the parents are better prepared and eager to help their children in professional self-determination on one hand, while teenagers make more thoughtful vocational choices, on the other hand. Significant results and conclusions are provided, to prove reliability of researching hypothesis, put forward by the author.

EFFECTS OF CASTRATION, AND TESTOSTERONE IN VITRO, ON THE HYPOTHALAMIC SYNTHESIS OF DIFFERENT PEPTIDE FRACTIONS

1975 ◽  
Vol 64 (1) ◽  
pp. 155-161 ◽  
Author(s):  
J. A. MOGUILEVSKY ◽  
M. A. ENERO ◽  
B. SZWARCFARB ◽  
D. DOSORETZ
Keyword(s):  
Luteinizing Hormone ◽  
Incubation Medium ◽  
Ammonium Acetate ◽  
Follicle Stimulating Hormone ◽  
The Other ◽  
Ovariectomized Rats ◽  
Small Peptides ◽  
Other Hand ◽  
Peptide Fractions

SUMMARY The incorporation of [3H]tyrosine ([3H]tyr) into different hypothalamic peptide fractions isolated from normal and castrated rats on a Sephadex G-25 column has been studied in vitro. Luteinizing hormone releasing factor (LH-RF) activity was determined in the different fractions by measuring their ability to elicit release of radioimmunoassayable luteinizing hormone (LH) and follicle-stimulating hormone (FSH) in ovariectomized rats treated with oestrogen and progesterone. For further purification, the fraction with LH-RF activity was applied to a CM-Sephadex G-25 column eluted with a gradient of ammonium acetate. The large radioactive peptides emerged from the Sephadex G-25 column in fraction S-1, while the small peptides with LH-RF activity were eluted in fraction S-2. Gonadectomy significantly increased the incorporation of [3H]tyr into the peptides of fractions S-2. Only in the purified fraction with LH-RF activity was the radioactivity incorporated higher in gonadectomized than in normal rats. The enhanced incorporation in fraction CM-3 observed after castration implies an increase in the hypothalamic synthesis of peptides with LH-RF activity. The addition of testosterone (2 μg/ml) to the incubation medium of hypothalamus from gonadectomized rats, corrected these modifications. Gonadectomy decreased the incorporation of tyrosine into the large peptides, and incubation with testosterone corrected this change. The modifications in the incorporation of [3H]tyr into the large and small peptides produced by castration appear to indicate that gonadectomy, as well as stimulating the production of LH-RF, enhances the synthesis of other hypothalamic peptides while inhibiting the synthesis of proteins. On the other hand an increase in the breakdown of large peptides into small peptides cannot be excluded.

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