SOMATOMEDIN A IN HUMAN SERUM, DETERMINED BY RADIORECEPTOR ASSAY

1976 ◽  
Vol 82 (2) ◽  
pp. 449-459 ◽  
Author(s):  
K. Takano ◽  
K. Hall ◽  
M. Ritzén ◽  
L. Iselius ◽  
H. Sievertsson
Keyword(s):  
Human Serum ◽  
Primary Hypothyroidism ◽  
Radioreceptor Assay ◽  
Turner's Syndrome ◽  
Turner’S Syndrome ◽  
Mean Values ◽  
The Mean ◽  
Low Levels

ABSTRACT Somatomedin A was determined by radioreceptor assay in serum from patients with various disorders. The mean values of somatomedin A in serum from 20 adult subjects, 21 patients with acromegaly, and 17 patients with hypopituitary dwarfism were 1.08 ± 0.08, 3.02 ± 0.33 and 0.44 ± 0.03 U/ml, respectively. In children below 2 years of age, low levels were found (mean 0.40 ± 0.04 U/ml). Normal levels were found in patients with Turner's syndrome and primary hypothyroidism, increased levels in uraemic patients, and decreased levels in Laron dwarfs.

scholarly journals Serum Levels of 20-Kilodalton Human Growth Hormone (GH) Are Parallel Those of 22-Kilodalton Human GH in Normal and Short Children

1999 ◽  
Vol 84 (1) ◽  
pp. 98-104 ◽  
Author(s):  
Mayumi Ishikawa ◽  
Susumu Yokoya ◽  
Katsuhiko Tachibana ◽  
Yukihiro Hasegawa ◽  
Toshiaki Yasuda ◽  
...  
Keyword(s):  
Body Mass Index ◽  
Short Stature ◽  
Physiological Role ◽  
Growth Disorders ◽  
Turner's Syndrome ◽  
Turner’S Syndrome ◽  
Nocturnal Sleep ◽  
Normal Children ◽  
Significant Difference ◽  
The Mean

Twenty-kilodalton human GH (20K), which is one of the human GH (hGH) variants, is thought to be produced by alternative premessenger ribonucleic acid splicing. However, its physiological role is still unclear due to the lack of a specific assay. We have measured serum 20K and 22-kDa hGH (22K) by specific ELISAs to investigate the physiological role of 20K in children. The subjects were 162 normal children, aged 1 month to 20 yr; 12 patients with GH deficiency (GHD), aged 11 months to 13 yr; 57 children with non-GHD short stature, aged 2–17 yr; and 13 girls with Turner’s syndrome, aged 5 months to 15 yr. Samples were collected at random from normal children and were collected after hGH provocative tests and 3-h nocturnal sleep from GHD, non-GHD short stature, and Turner’s syndrome children. The mean basal serum concentrations of 22K and 20K were 2.4 ± 2.8 ng/mL and 152.3 ± 184.0 pg/mL in normal boys and 2.5 ± 3.1 ng/mL and 130.6 ± 171.5 pg/mL in normal girls, respectively. The percentages of 20K (%20K) were 5.8 ± 2.1% and 6.0 ± 3.2% in 83 normal boys and 79 normal girls, respectively. There was no significant difference in %20K either among ages or between the prepubertal stage and the pubertal stage in normal boys and girls. The mean %20K values in basal samples of provocative tests in 12 patients with GHD, non-GHD short stature, and Turner’s syndrome were 6.5 ± 2.4%, 6.5 ± 3.8%, and 5.9 ± 3.2%, respectively. There was no significant difference in %20K among normal children and these growth disorders, and there was no significant difference in %20K throughout the hGH provocative tests and 3-h nocturnal sleep in these growth disorders. There was also no significant correlation between the percentage of 20K and the height sd score or body mass index in either normal children or subjects with these growth disorders. In conclusion, the %20K is constant, regardless of age, sex, puberty, height sd score, body mass index, and GH secretion status. The regulation of serum 20K levels remains to be established.

Intra-individual variation of thyroxin, triiodothyronine, and thyrotropin in treated hypothyroid patients: implications for monitoring replacement therapy.

1988 ◽  
Vol 34 (4) ◽  
pp. 696-699 ◽  
Author(s):  
M C Browning ◽  
W M Bennet ◽  
A J Kirkaldy ◽  
R T Jung
Keyword(s):  
Primary Hypothyroidism ◽  
Free Triiodothyronine ◽  
Post Dose ◽  
Strict Adherence ◽  
Mean Values ◽  
Specimen Collection ◽  
Total Triiodothyronine ◽  
The Mean ◽  
Random Fluctuations ◽  
Hypothyroid Patients

Abstract We measured total thyroxin (TT4), free thyroxin (FT4), total triiodothyronine (TT3), free triiodothyronine (FT3), and thyrotropin (TSH) in serum sampled before and 1, 2, 4, 6, and 8 h after administration of prescribed doses of thyroxin to 12 patients with proven primary hypothyroidism. At 2, 4, and 6 h post-dose, the mean values for TT4 and FT4 and also that at 8 h for FT4 significantly (P less than 0.05) exceeded the corresponding pre-dose values. No significant changes were found for TT3, FT3, or TSH. The mean intra-individual CVs over the study period were TT4 4.9%, FT4 5.7%, TT3 8.7%, FT3 8.7%, and TSH 20.2%. Individual subjects showed small but predictable changes in TT4 and FT4. Changes in TT3 and FT3 were greater but random. Fluctuations in TSH were greatest, but in all subjects with detectable concentrations the variations were of similar magnitude. We conclude that strict adherence to timing of specimen collection in relation to dosage is probably unnecessary.

Effects of luteinizing hormone-releasing hormone (LRH) upon bioactive and immunoreactive serum LH in patients with Turner's syndrome before and after oestrogen treatment

1985 ◽  
Vol 109 (3) ◽  
pp. 304-308 ◽  
Author(s):  
Marco Francesco Celani ◽  
Vanna Montanini ◽  
Paolo Marrama
Keyword(s):  
Biologically Active ◽  
Delayed Response ◽  
Turner's Syndrome ◽  
Turner’S Syndrome ◽  
Oestrogen Treatment ◽  
Serum Lh ◽  
Before And After ◽  
The Mean ◽  
Ethinyl Oestradiol

Abstract. One daily dose of 0.05 mg ethinyl oestradiol was administered to 5 patients with Turner's syndrome (mean age ± sem = 16.4 ± 0.7 years) for 10 days. The effects of acute stimulation with luteinizing hormonereleasing hormone (LRH) (0.1 mg iv) on biologically active and immunoreactive LH were analysed before therapy and at the end of oestrogen treatment. Bioactive LH (BIO-LH) was measured by a sensitive and specific in vitro bioassay based upon testosterone production by mechanically dispersed mouse Leydig cell preparations. Immunoreactive LH (RIA-LH) was evaluated by a double antibody RIA method. Prior to oestrogen treatment, LRH induced a prompt rise in BIO-LH and RIA-LH levels, which reached peak values at 30 and 45 min, respectively. After oestrogen treatment, a delayed response (with peak values at 120 min) was observed for both BIO-LH and RIA-LH. Before oestrogen treatment, the mean bioactivity to immunoreactivity (B/I) ratio of LRH-stimulated LH showed a significant decrease from basal values (P < 0.05). In contrast, after ethinyl oestradiol administration the mean LH B/I ratio increased significantly from basal values in response to LRH (P < 0.05). The mean relative maximum response (Δ%) for BIO-LH was significantly higher (P < 0.05) in oestrogen-treated than in untreated patients, whereas the mean BIO-LH Δ area was significantly lower in the former group (P < 0.01). Similarly, oestrogens decreased significantly the mean RIA-LH Δ area (P < 0.05), whereas they did not affect significantly the mean RIA-LH Δ%. The results further emphasize that oestrogens may change the quality of circulating LH.

THE INTERACTION OF GROWTH HORMONE, SOMATOMEDIN AND OESTROGEN IN PATIENTS WITH TURNER'S SYNDROME

1976 ◽  
Vol 81 (1) ◽  
pp. 9-18 ◽  
Author(s):  
Paul Saenger ◽  
Ernest Schwartz ◽  
Eckehart Wiedemann ◽  
Lenore S. Levine ◽  
Mary Tsai ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Negative Feedback ◽  
Hormone Secretion ◽  
Growth Hormone Secretion ◽  
Turner's Syndrome ◽  
Oestrogen Therapy ◽  
Turner’S Syndrome ◽  
Normal Limits ◽  
Control Serum ◽  
The Mean

ABSTRACT Baseline somatomedin activity in seven of eight patients with Turner's syndrome was found to be within normal limits. Somatomedin activity readily suppressed with oestrogen administration. The overall mean serum somatomedin activity during oestrogen therapy (0.87 U/ml, sd 0.15) was significantly lower (P < 0.005) than the mean of the control serum somatomedin activities (1.09 U/ml, sd 0.24). During oestrogen therapy, suggestive elevations of fasting growth hormone levels were noted in five of eight patients. The data indicate that oestrogenic suppression of serum somatomedin was not due to decreased growth hormone secretion and suggest the existence of a negative feedback link between somatomedin and growth hormone.

Measurement of nocturnal urinary growth hormone values

1989 ◽  
Vol 121 (2) ◽  
pp. 290-296 ◽  
Author(s):  
Izumi Sukegawa ◽  
Naomi Hizuka ◽  
Kazue Takano ◽  
Kumiko Asakawa ◽  
Reiko Horikawa ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Gh Deficiency ◽  
Turner's Syndrome ◽  
Turner’S Syndrome ◽  
Gh Secretion ◽  
Short Children ◽  
The Mean ◽  
Plasma Gh ◽  
Normal Adults ◽  
Collection Time

Abstract. Nocturnal urinary growth hormone values were measured by a sensitive enzyme immunoassay in normal adults, patients with GH deficiency, patients with Turner's syndrome, normal but short children who had normal plasma GH responses to provocative tests, and patients with acromegaly. The mean nocturnal urinary GH values in patients with acromegaly were significantly greater than those in normal adults (1582.3 ± 579.8 vs 53.5 ± 8.6 pmol/mmol creatinine (± sem); p < 0.05). In the normal but short children and patients with Turner's syndrome, the mean nocturnal urinary GH values were 83.1 ± 5.2 and 79.8 ± 29.5 pmol/mmol creatinine, respectively. In patients with GH deficiency, the nocturnal urinary GH values were undetectable (< 5.3 pmol/mmol creatinine) except in one patient where the value was 6.3 pmol/mmol creatinine. The nocturnal urinary GH values of the patients with GH deficiency were significantly lower than those of the other groups (p < 0.05). In normal but short children, the nocturnal urinary GH values correlated significantly with mean plasma nocturnal GH concentrations (r = 0.76, p < 0.001), and 24-hour urinary GH values (r = 0.84, p < 0.001), respectively. In 4 patients with GH deficiency who had circulating anti-hGH antibody, the urinary GH values were also undectable. These data indicate that nocturnal urinary GH value reflects endogenous GH secretion during collection time, and that measurement of the nocturnal urinary GH values is a useful method for screening of patients with GH deficiency and acromegaly.

PLATELET CONTENTS OF VON WILLEBRAND FACTOR ANTIGEN (vWF:Ag) AND FIBRINOGEN (I) AND PLATELET FUNCTION (PF) IN MYELODYSPLASTIC SYNDROMS (MS)

1987 ◽  
Author(s):  
S Meschengieser ◽  
A Blanco ◽  
N Maugeri ◽  
J Fernandez ◽  
M de Tezanos Pinto ◽  
...  
Keyword(s):  
Bleeding Time ◽  
Refractory Anemia ◽  
Sideroblastic Anemia ◽  
Mean Values ◽  
Normal Platelet ◽  
The Mean ◽  
Low Levels ◽  
Von Willebrand ◽  
Willebrand Factor ◽  
Factor Antigen

Bleeding in MS is usually associated with thrombocytopenia, but may occur in patients (P) with a normal platelet count (PC) and defects in the release (R) reaction and the arachidonic acid (AA) pathway. Weinvestigated the PF and contents of vWF:Ag and I in 6 P with diagnosis of: Acquired Idiopathic Sideroblastic Anemia (2), Refractory Anemia (2) and Refractory Anemia with Excess of Blasts (2). Laboratory testsincluded: PC; bleeding time (BT)(Ivy); platelet retention to glass beads (PR); platelet aggregation (PA)and R induced by ADP(2.5juM), epinephrine (E)(0.1mM), collagen (C) (l-8ug/ml), AA(0.8-lmM) and A23187 (A)(19jjM); spontaneous PA (SPA); generation (G) and response (Re) to thromboxane A2 like material (TXA2);vWF:Ag and I (electroimmunoassay)in platelet lysates. Results: PC were normal in 6/6, BT were prolonged in 3/6, PR were reduced in 4/6 , PA induced by E were absent in 4/6 and abnormal in the other 2, absenceof secondary PA induced by ADP were observed in 4/6, PA induced by C and AA were abnormal in 5/6. No SPA were observed in 6/6. ATP R were absent with ADP andE in 4/6, with C and AA in 5/6 and abnormal with A in 2/6. The G of TXA2 were absent or decreased and the Re to_TXA2 were normal in all the P. vWF:Ag (X: 0.088U/109Cells) and I (X: 16.8jμg/109cells) were de-creased in_6/6 P and the mean values were lower (p<0.001) than controls (X: 0.284U/109cells and X: 61.2jug/109cells respectively). The PF abnormalities observed are similar to those described by other authors, but the decrease of vWF:Ag and I have not been previously reported.These low levels could be the resultof platelet activation or megacaryocyte dys function.The re was no evidence of platelet hyperactivity inour P. So, we assume that defective megacaryocytopoiesis with an alteration in the storage of the granules contents could be responsible for the depletion observed.

Scanning and Transmission Electron Microscopy of the Endometrium in Women with Ovarian Dysgenesis (Turner'S Syndrome)

1976 ◽  
Vol 34 ◽  
pp. 148-149
Author(s):  
A. González-Angulo ◽  
S. Armendares-Sagrera ◽  
I. Ruíz de Chávez ◽  
H. Marquez-Monter ◽  
R. Aznar
Keyword(s):  
Surface Epithelium ◽  
Secretory Cells ◽  
Turner's Syndrome ◽  
Ciliated Cells ◽  
Turner’S Syndrome ◽  
Exogenous Hormone ◽  
Ovarian Dysgenesis ◽  
Transmission Electron ◽  
Normal Women ◽  
Microscopy Examination

It is a well documented fact that endometrial hyperplasia and adenocarcinoma may develop in women with Turner's syndrome who had received unopposed estrogen treatment (1), as well as in normal women under contraceptive medication with the sequential regime (2). The purpose of the present study was to characterize the possible changes in surface and glandular epithelium in these women who were treated with a sequential regime for a period of between three and eight years. The aim was to find organelle modifications which may lead to the understanding of the biology of an endometrium under exogenous hormone stimulation. Light microscopy examination of endometrial biopsies of nine patients disclosed a proliferative pattern; in two of these, there was focal hyperplasia. With the scanning electron microscope the surface epithelium in all biopsies showed secretory cells with microvilli alternating with non secretory ciliated cells. Regardless of the day of the cycle all biopsies disclosed a large number of secretory cells rich in microvilli (fig.l) with long and slender projections some of which were branching (fig. 2).

Articulatory Behavior Pre and Post Full-Mouth Tooth Extraction and Alveoloplasty

1980 ◽  
Vol 23 (3) ◽  
pp. 630-645 ◽  
Author(s):  
Gerald Zimmermann ◽  
J.A. Scott Kelso ◽  
Larry Lander
Keyword(s):  
Control Subject ◽  
High Speed ◽  
Tooth Extraction ◽  
Kinematic Parameters ◽  
Experimental Conditions ◽  
Mean Values ◽  
Articulatory Movements ◽  
The Mean ◽  
Experimental Subjects ◽  
Normal Speech

High speed cinefluorography was used to track articulatory movements preceding and following full-mouth tooth extraction and alveoloplasty in two subjects. Films also were made of a control subject on two separate days. The purpose of the study was to determine the effects of dramatically altering the structural dimensions of the oral cavity on the kinematic parameters of speech. The results showed that the experimental subjects performed differently pre and postoperatively though the changes were in different directions for the two subjects. Differences in both means and variabilities of kinematic parameters were larger between days for the experimental (operated) subjects than for the control subject. The results for the Control subject also showed significant differences in the mean values of kinematic variables between days though these day-to-day differences could not account for the effects found pre- and postoperatively. The results of the kinematic analysis, particularly the finding that transition time was most stable over the experimental conditions for the operated subjects, are used to speculate about the coordination of normal speech.

Comparative Study of Intranasal, Subcutaneous and Intravenous Administration of Desamino-D-Arginine Vasopressin (DDAVP)

1986 ◽  
Vol 55 (01) ◽  
pp. 108-111 ◽  
Author(s):  
M Köhler ◽  
P Hellstern ◽  
C Miyashita ◽  
G von Blohn ◽  
E Wenzel
Keyword(s):  
Factor Viii ◽  
Von Willebrand Factor ◽  
Factor Xii ◽  
Additional Advantage ◽  
Mean Values ◽  
Routes Of Administration ◽  
The Mean ◽  
Von Willebrand ◽  
Willebrand Factor ◽  
Factor Antigen

SummaryThis study was performed to evaluate the influence of different routes of administration on the efficacy of DDAVP treatment. Ten healthy volunteers received DDAVP intranasally (i.n.), subcutaneously (s.c.) and intravenously (i.v.) in a randomized cross-over trial. Factor XII and high molecular weight (HMW)-kininogen levels increased only slightly after DDAVP administration. The mean increase of factor VIII: C was 3.1 (i. v.), 2.3 (s. c.), and 1.3 (i.n.) - fold over baseline. Ristocetin cofactor (von Willebrand factor antigen) increased 3.1 (2.5), 2.0 (2.3) and 1.2 (1.2) - fold over baseline mean values after i.v., s.c. and i.n. DDAVP, respectively. The half-disappearance time of factor VIII and von Willebrand factor (vWF) after DDAVP ranged from five (factor VIII: C) to eight hours (vWF). The mean increase of fibrinolytic activity was more pronounced after i.v. DDAVP. The antidiuretic effect was moderate with no apparent differences between the routes of application. This study provides further evidence that both i.v. and s.c. DDAVP administration result in an appropriate and reliable stimulation of haemostasis. An additional advantage of s. c. administration is its suitability for home treatment.

Comparative Study of effectiveness of mobilization with movement (MWM) and End range mobilization (ERM) techniques in frozen shoulder

2018 ◽  
Vol 4 (4) ◽  
pp. 519-522
Author(s):  
Jeyakumar S ◽  
Jagatheesan Alagesan ◽  
T.S. Muthukumar
Keyword(s):  
Range Of Motion ◽  
Frozen Shoulder ◽  
Type I ◽  
Mean Values ◽  
Functional Activities ◽  
Group A ◽  
The Mean ◽  
Group B

Background: Frozen shoulder is disorder of the connective tissue that limits the normal Range of motion of the shoulder in diabetes, frozen shoulder is thought to be caused by changes to the collagen in the shoulder joint as a result of long term Hypoglycemia. Mobilization is a therapeutic movement of the joint. The goal is to restore normal joint motion and rhythm. The use of mobilization with movement for peripheral joints was developed by mulligan. This technique combines a sustained application of manual technique “gliding” force to the joint with concurrent physiologic motion of joint, either actively or passively. This study aims to find out the effects of mobilization with movement and end range mobilization in frozen shoulder in Type I diabetics. Materials and Methods: 30 subjects both male and female, suffering with shoulder pain and clinically diagnosed with frozen shoulder was recruited for the study and divided into two groups with 15 patients each based on convenient sampling method. Group A patients received mobilization with movement and Group B patients received end range mobilization for three weeks. The outcome measurements were SPADI, Functional hand to back scale, abduction range of motion using goniometer and VAS. Results: The mean values of all parameters showed significant differences in group A as compared to group B in terms of decreased pain, increased abduction range and other outcome measures. Conclusion: Based on the results it has been concluded that treating the type 1 diabetic patient with frozen shoulder, mobilization with movement exercise shows better results than end range mobilization in reducing pain and increase functional activities and mobility in frozen shoulder.

Sign in / Sign up

Export Citation Format

Share Document