THE EFFECT OF ACTH ON PLASMA TESTOSTERONE AND ANDROSTENEDIONE CONCENTRATIONS IN PATIENTS WITH PROSTATIC CARCINOMA

1976 ◽  
Vol 81 (2) ◽  
pp. 310-320 ◽  
Author(s):  
Teresa H. Cowley ◽  
B. G. Brownsey ◽  
M. E. Harper ◽  
W. B. Peeling ◽  
K. Griffiths
Keyword(s):  
Plasma Concentration ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Endocrine Therapy ◽  
Prostatic Carcinoma ◽  
Plasma Testosterone ◽  
Testosterone Concentration ◽  
Carcinoma Of The Prostate ◽  
Specific Radioimmunoassay ◽  
Layer Chromatography

ABSTRACT The effect of Synacthen (β1–24-corticotrophin) on plasma testosterone and 4-androstene-3,17-dione concentrations in untreated patients with prostatic carcinoma, and in patients receiving endocrine therapy is described. An established specific radioimmunoassay was used for the measurement of testosterone, and a radioimmunoassay for 3-androstene-3,17-dione using thin layer chromatography has been developed. Administration of Synacthen resulted in a fall in testosterone in untreated patients, but a rise in 4-androstene-3,17-dione was observed. The plasma concentration of testosterone in all treated patients increased after administration of Synacthen. An increased concentration of plasma 4-androstene-3,17-dione was also observed in these treated patients after Synacthen, but the magnitude of the response was not significantly different from that of untreated patients. The work provides further evidence that in the patient being treated with oestrogen for carcinoma of the prostate a rise in plasma testosterone concentration will result from an increased secretion of ACTH.

Plasma Testosterone Using Sephadex LH-20 and Saturation Analysis by Competitive Protein Binding

1973 ◽  
Vol 10 (1-6) ◽  
pp. 115-119 ◽  
Author(s):  
Daphne M. Lawrence ◽  
G. I. Swyer
Keyword(s):  
Protein Binding ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Plasma Testosterone ◽  
Short Columns ◽  
Competitive Protein Binding ◽  
Layer Chromatography

A method Tor measuring plasma testosterone using separation on short columns of Sephadex LH-20 and competitive protein binding is described. This is more reliable and practicable than methods using paper or thin layer chromatography separation and Sephadex LH-20 can be re-used after washing.

scholarly journals Determination of Cortisol in Human Plasma by Radioimmunoassay

1977 ◽  
Vol 14 (1-6) ◽  
pp. 343-349 ◽  
Author(s):  
G. F. Read ◽  
Diana R. Fahmy ◽  
R. F. Walker
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Human Plasma ◽  
Plasma Cortisol ◽  
Specific Radioimmunoassay ◽  
Precision And Accuracy ◽  
Layer Chromatography

A radioimmunoassay for plasma cortisol featuring the gamma-emitting radioligand 125I-iodohistamine, coupled to cortisol-3-(O-carboxymethyl)-oxime, is described. The new procedure retains much of the specificity associated with the use of anti-cortisol-3-BSA sera with tritium-labelled radioligands, and has the further advantages that running costs are lower and there is a greater potential for automation. Cortisol values obtained by this procedure agree well with those obtained by a published specific radioimmunoassay using the tritiated cortisol radioligand. Specificity of the procedure was checked by comparing values obtained with and without thin-layer chromatography purification: correlation was excellent (r = 0·96). Satisfactory levels of sensitivity, precision, and accuracy were obtained.

18-Hydroxycorticosterone as a Marker for Primary Hyperaldosteronism

1989 ◽  
Vol 26 (3) ◽  
pp. 227-232 ◽  
Author(s):  
Ruth Lapworth ◽  
Frances Short ◽  
V H T James
Keyword(s):  
Plasma Concentration ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Primary Hyperaldosteronism ◽  
Adrenal Hyperplasia ◽  
Control Subjects ◽  
Bilateral Adrenal Hyperplasia ◽  
Layer Chromatography ◽  
Mean Concentration

An assay for the measurement of 18-hydroxycorticosterone (18-OHB) in plasma has been validated. The method involves extraction of plasma with dichloro-methane, thin layer chromatography and radioimmunoassay with an iodinated 18-hydroxycorticosterone-3-carboxymethyloxime ligand. The plasma concentration of 18-OHB was measured in 16 patients with primary hyperaldosteronism and 20 control subjects. After overnight recumbency a significantly higher mean concentration of 18-OHB was found in the samples taken from the patients than in those from the control subjects. The degree of elevation did not clearly discriminate between patients with a unilateral adenoma and those with bilateral adrenal hyperplasia. A significant anomalous postural decrease in the 18-OHB concentration occurred in 10 of the 13 patients with an adenoma, whereas there was a significant postural increase in the three patients with hyperplasia.

DIURNAL VARIATION IN PLASMA GLUCOCORTICOSTEROID LEVELS IN THE HORSE (EQUUS CABALLUS)

1966 ◽  
Vol 35 (3) ◽  
pp. 249-253 ◽  
Author(s):  
A. ZOLOVICK ◽  
D. W. UPSON ◽  
B. E. ELEFTHERIOU
Keyword(s):  
Plasma Concentration ◽  
Thin Layer ◽  
Diurnal Variation ◽  
Thin Layer Chromatography ◽  
Sulphuric Acid ◽  
Equus Caballus ◽  
Maximum Plasma ◽  
Free Cortisol ◽  
Layer Chromatography ◽  
Free Plasma

SUMMARY Thin-layer chromatography, acetylation of reference and unidentified glucocorticosteroids, u.v. absorption and fluorescence induced in sulphuric acid were used to identify cortisol as the major free plasma glucocorticosteroid in the horse (Equus caballus), with cortisone and corticosterone as minor glucocorticosteroids. Deoxycorticosterone was also identified. The plasma ratio for free cortisol: cortisone: corticosterone was 16:8:0·5. The diurnal variation was determined for all three glucocorticosteroids. The highest levels of cortisol and corticosterone were found at 10.00 hr. (260 and 10·3 μg./100 ml., respectively) and the lowest concentration of cortisol at 02.00 hr. The highest level of cortisone occurred at 02.00 hr. (140 μg./100 ml.), and the lowest appeared at 16.00 hr. (65 μg./100 ml.). The maximum plasma concentration of the combined glucocorticosteroids was found at 10.00 hr. (395·3 μg./100 ml.), and the minimum at 20.00 hr. (219 μg./100 ml.).

Thin-Layer Chromatography

1965 ◽  
Author(s):  
H. R. Bolliger ◽  
M. Brenner ◽  
H. Gänshirt ◽  
Helmut K. Mangold ◽  
H. Seiler ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Layer Chromatography

THE LESS-POLAR METABOLITES PRODUCED BY INCUBATION OF TESTOSTERONE-4-14C WITH RAT AND BOVINE BRAIN

1969 ◽  
Vol 61 (4) ◽  
pp. 641-648 ◽  
Author(s):  
Leon J. Sholiton ◽  
Emile E. Werk
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Paper Chromatography ◽  
Specific Activity ◽  
Bovine Brain ◽  
Polar Fraction ◽  
End Products ◽  
Polar Metabolites ◽  
Layer Chromatography ◽  
Constant Specific Activity

ABSTRACT Rat and bovine brain have been incubated with testosterone-4-14C under standard conditions. With use of paper chromatography, the extracted metabolites were noted to fall into less-polar, iso-polar, and more polar fractions. The components of the less-polar fraction were separated by acetylation and thin-layer chromatography and the major end-products identified by recrystallization to constant specific activity or constant 3H/14C ratios. Androst-4-enedione and 5α-dihydrotestosterone were formed consistently under the conditions utilized. Trace amounts of other less-polar metabolites were noted occasionally.

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