APPLICATION OF A RADIOLIGAND RECEPTOR ASSAY FOR THE DETERMINATION OF LUTEINIZING HORMONE IN HUMAN SERUM

1975 ◽  
Vol 80 (1_Suppla) ◽  
pp. S127
Author(s):  
F. Leidenberger ◽  
R. Willaschek ◽  
V. Pahnke
Keyword(s):  
Luteinizing Hormone ◽  
Human Serum ◽  
Receptor Assay ◽  
Radioligand Receptor Assay

DETERMINATION OF LUTEINIZING HORMONE IN BOVINE BLOOD BY RADIOLIGAND RECEPTOR ASSAY AND COMPARISON WITH RADIOIMMUNOLOGICAL EVALUATION

1978 ◽  
Vol 87 (2) ◽  
pp. 268-278 ◽  
Author(s):  
D. Schams ◽  
C. Menzer
Keyword(s):  
Luteinizing Hormone ◽  
Close Correlation ◽  
Exchange Chromatography ◽  
Bovine Blood ◽  
Serum Samples ◽  
Bovine Plasma ◽  
Receptor Assay ◽  
C 50 ◽  
Radioligand Receptor Assay

ABSTRACT A sensitive and specific radioligand receptor assay (RRA) using rat testis homogenate as the receptor source is described for measurement of luteinizing hormone (LH) in bovine blood. Interfering and non-specific substances in blood were removed by means of ion-exchange chromatography on CM-Sephadex C-50. Criteria of validation such as recovery of added LH to plasma or serum, reproducibility, and specificity gave good results. Inhibition curves obtained with bovine plasma and serum were parallel to those obtained with the bovine standard preparation. The range of the dose-response curve was between 0.5–20 ng of bovine LH. The pattern of LH concentrations in purified serum samples under different physiological conditions such as during the oestrous cycle and after administration of GnRH showed a very close correlation whether measured by means of radioimmunoassay (RIA) or receptor assay. Values of RRA-LH were consistently higher than those of RIALH. Thus the lower the RIA-LH levels, the more pronounced were the discrepancies between results of both assay systems. The mean ratio of RRA-LH/RIA-LH for basal levels (less than 1 ng RIA-LH/ml plasma) was 17.8 as compared to a mean ratio for higher peak values (more than 20 ng RIA-LH/ml plasma) of only 1.2.

APPLICATION OF A RADIOLIGAND RECEPTOR ASSAY FOR DETERMINATION OF LUTEINIZING HORMONE IN HUMAN SERUM

1976 ◽  
Vol 81 (1) ◽  
pp. 54-72 ◽  
Author(s):  
F. A. Leidenberger ◽  
R. Willaschek ◽  
V. G. Pahnke ◽  
L. E. Reichert
Keyword(s):  
Human Serum ◽  
Precipitation Method ◽  
Serum Samples ◽  
Serum Lh ◽  
Fractionation Procedure ◽  
Receptor Assay ◽  
Menopausal Women ◽  
Post Menopausal ◽  
Radioligand Receptor Assay ◽  
Lh Releasing Hormone

ABSTRACT A sensitive, specific and economic radioligand receptor assay is described for measurement of LH in human serum (RRA-LH). By means of ethanol fractionation or (NH4)2SO4-precipitation serum can be prepared for LH-quantitation and tested in the RRA-LH without interference of a nonspecific inhibiting substance present in untreated serum from various human sources. Treatment of serum with 8 % ethanol separates nonspecific inhibiting substances from LH, the latter remaining in the supernatant at this ethanol concentration. The criteria of specificity are examined. The results of experiments designed to produce evidence for or against specificity suggest specificity of the RRA-LH. Recoveries, as estimated by administration of [125I]hLH and unlabelled hLH to untreated serum samples are shown to be between 80 and 95 % for the ethanol fractionation procedure and between 65 and 75 % for the (NH4)2SO4-precipitation method. The ethanol fractionation procedure is preferred for routine serum-LH determination because of its simplicity, speed and higher recoveries. Ethanol-treated sera from post-menopausal women show, on average, higher RRA-LH concentrations than ethanol-treated sera from young women. RRA-LH values are consistently higher than LH-values found by radioimmunoassay (RIA-LH). The LH-concentrations in sera from two menstrual cycles and from two LH-releasing hormone tests are measured by RRA-LH and by RIA-LH. Similarities and discrepancies of the LH-profiles found by the two assay systems are described.

Performance Evaluation of an Improved ARCHITECT LH Assay for the Quantitative Determination of Luteinizing Hormone in Human Serum and Plasma

BIO ◽  
2012 ◽  
Vol 2 (1) ◽  
pp. 27-36
Author(s):  
Michael Oer ◽  
Christian Beckert ◽  
Boris Heinrich ◽  
Carsten Buenning ◽  
Hans-Peter Kapprell
Keyword(s):  
Performance Evaluation ◽  
Luteinizing Hormone ◽  
Quantitative Determination ◽  
Human Serum
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