CONDITIONS INFLUENCING THE DOSE-RESPONSE EFFECT OF GROWTH HORMONE ON LONGITUDINAL BONE GROWTH IN THE HYPOPHYSECTOMIZED RAT

1975 ◽  
Vol 80 (2) ◽  
pp. 284-296 ◽  
Author(s):  
K.-G. Thorngren ◽  
L. I. Hansson
Keyword(s):  
Growth Hormone ◽  
Dose Response ◽  
Growth Response ◽  
Bone Growth ◽  
Practical Importance ◽  
Longitudinal Bone Growth ◽  
Response Effect ◽  
Dependent Growth ◽  
Dose Response Effect ◽  
Dose Dependent

ABSTRACT Various factors that might influence the dose-response effect of growth hormone on the longitudinal bone growth were investigated with tetracycline as intravital marker of the bone growth in hypophysectomized rats. These are of practical importance for the bioassay of growth hormone. The growth response was found to be almost the same for subcutaneous and intraperitoneal injection, whereas the intravenous route resulted in significantly lower growth response. The administration of growth hormone in various volumes subcutaneously did not significantly influence the dose-dependent growth response. Freezing of the dissolved growth hormone or addition of NaOH to the solution had no significant effect on the growth response. Cortisone acetate 0.5 mg/kg given at hypophysectomy increased the post-operative survival somewhat, without having any post-operative depressing influence on the growth hormone-induced longitudinal bone growth. The present investigation showed that in standardized conditions the dose-dependent growth response is constant.

THE BIOASSAY OF GROWTH HORMONE IN SNELL'S DWARF MICE: EFFECTS OF THYROXINE AND PROLACTIN ON THE DOSE—RESPONSE CURVE

1973 ◽  
Vol 56 (2) ◽  
pp. 235-243 ◽  
Author(s):  
M. WALLIS ◽  
JENNIFER A. DEW
Keyword(s):  
Growth Hormone ◽  
Response Curve ◽  
Growth Response ◽  
Growth Promotion ◽  
Promoting Effect ◽  
Pituitary Growth Hormone ◽  
Growth Promoting ◽  
Dependent Growth ◽  
Dwarf Mice ◽  
Dose Dependent

SUMMARY Pituitary growth hormone has a dose-dependent growth promoting effect in pituitary dwarf mice (Snell's strain), and this effect can be used as the basis of a bioassay for the hormone. Prolactin and thyroxine also promote growth in these animals, and the effects of these hormones in combination with growth hormone were studied, in order to see whether their presence might enhance the precision or sensitivity of the growth hormone assay. When prolactin and/or thyroxine were administered with growth hormone, the growth response observed was no greater than the sum of the effects of the hormones given separately; in some cases it was less. Neither prolactin nor thyroxine increase the sensitivity or precision of the growth hormone bioassay. The implications of these results for theories about the mechanisms of growth promotion by these hormones are considered.

EFFECT OF DOSE AND ADMINISTRATION PERIOD OF GROWTH HORMONE ON LONGITUDINAL BONE GROWTH IN THE HYPOPHYSECTOMIZED RAT

1973 ◽  
Vol 74 (1) ◽  
pp. 1-23 ◽  
Author(s):  
K.-G. Thorngren ◽  
L. I. Hansson ◽  
K. Menander-Sellman ◽  
A. Stenström
Keyword(s):  
Growth Hormone ◽  
Growth Plate ◽  
Dose Response ◽  
Bone Growth ◽  
Growth Parameters ◽  
Growth Stimulation ◽  
The Body ◽  
Sprague Dawley ◽  
Longitudinal Bone Growth ◽  
Response Determination

ABSTRACT The effect of bovine growth hormone (NIH-GH-B15) on the growth in length of the proximal growth plate of the tibia in hypophysectomized female Sprague-Dawley rats was studied by the tetracycline method. The width of the growth plate was also determined and the weight of the body and heart was registered. The completeness of the hypophysectomy was determined microscopically. The daily sc injection of 25 μg NIH-GH-B15 for 10, 20 or 30 days resulted in an increasing growth in length with increasing administration period. When various doses (5, 25, 100 or 400 μg) NIH-GH-B15 were administered daily for 20 days, the growth in length increased with the dose. A single injection of 45 mg/kg cortisone acetate given at hypophysectomy depressed the growth stimulation of growth hormone. The age at hypophysectomy also influenced the growth hormone-induced growth stimulation. Animals hypophysectomized at 40 days of age had a higher growth in length for the same doses and administration periods of growth hormone than those operated at 60 days of age. The width of the growth plate of the proximal tibia and the weight of the body and heart responded in a similar manner as the longitudinal bone growth to various doses and administration periods of growth hormone, but the changes were less obvious. The dose-response relation after the administration of various doses of growth hormone for 20 days was tested for different growth parameters by the index of precision (λ). Of the growth parameters in this investigation, the accumulated growth in length in animals hypophysectomized at 60 days of age without cortisone at operation was found to be most suitable for dose-response determination of growth hormone. The intention is to develop a bio-assay for growth hormone.

Is Speed of Onset of Anti-anxiety Efficacy with Pregabalin Influenced by Starting Dose?

2009 ◽  
Vol 24 (S1) ◽  
pp. 1-1
Author(s):  
B. Herman ◽  
F. Mandel
Keyword(s):  
Dose Response ◽  
Change Score ◽  
Early Improvement ◽  
Response Effect ◽  
Starting Dose ◽  
Dsm Iv ◽  
The Mean ◽  
Dose Response Effect ◽  
Titration Schedule ◽  
Speed Of Onset

Objective:There appears to be no dose-response effect for pregabalin at doses of 300-600 mg, and a modest dose-response effect in the range of 150-300 mg. The goal of the current investigation was to determine the effect of the starting dose on the speed of onset of anxiolytic efficacy.Methods:Data were analyzed from 7 trials of outpatients with DSM-IV GAD and a HAM-A total score ≥18. Starting doses of pregabalin ranged from 100 mg (N=301) or 150 mg (N=104), to 200 mg (N=167) and 300 mg (N=388). Assessment of early improvement included the HAM-A total score and CGI-Severity and Improvement scores.Results:The mean Week 1 HAM-A change score was similar for a starting dose of 200 mg/d with no titration (-8.24) when compared to patients who started on 200 mg/d and then titrated up to 400 mg/d on Day 4 (-8.64). The mean Week 1 HAM-A change score was somewhat higher for patients started on 300 mg/d, and then titrated to 450 mg/d on Day 4/5 (-8.84) when compared to patients started on a lower (100/150 mg/d) dose and titrated on Day 5 to 400/450 mg/d (-7.32). Starting on a dose of 300 mg/d with no titration resulted in an intermediate Week 1 change score (-7.87). The interaction of starting dose and titration schedule with baseline anxiety severity will be summarized in detail.Conclusion:The initial dose of pregabalin appears to have only a weak effect on the speed of onset of anxiolytic improvement.

A single dose-response effect of aflatoxin B1 on rapid liver cancer induction in two strains of rats

1990 ◽  
Vol 46 (4) ◽  
pp. 664-668 ◽  
Author(s):  
Subhkij Angsubhakorn ◽  
Panisa Get-Ngern ◽  
Makoto Miyamoto ◽  
Natth Bhamarapravati
Keyword(s):  
Single Dose ◽  
Liver Cancer ◽  
Dose Response ◽  
Aflatoxin B1 ◽  
Response Effect ◽  
Cancer Induction ◽  
Dose Response Effect

The Dose–Response Effect of Lycopene on Cerebral Vessel and Neuron Impairment Induced by Hyperlipidemia

2018 ◽  
Vol 66 (50) ◽  
pp. 13173-13182 ◽  
Author(s):  
Wei Wang ◽  
Weichun Yang ◽  
Ziyi Shen ◽  
Sixian Wen ◽  
Minyu Hu
Keyword(s):  
Dose Response ◽  
Cerebral Vessel ◽  
Response Effect ◽  
Dose Response Effect
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