EFFECT OF PROGESTERONE ON RNA AND PROTEIN SYNTHESIS IN THE RAT UTERUS

1973 ◽  
Vol 73 (4) ◽  
pp. 740-750 ◽  
Author(s):  
G. Trams ◽  
H. Brewitt ◽  
H. Möllmann ◽  
H. Maass
Keyword(s):  
Protein Synthesis ◽  
Nucleic Acid ◽  
Cell Membrane ◽  
Inhibitory Effect ◽  
Rat Uterus ◽  
Isotope Labelling ◽  
Uridine Incorporation ◽  
Rna And Protein Synthesis ◽  
Modifying Effect

ABSTRACT The effect of gestagenic compounds on the oestrogen induced nucleic acid and protein synthesis of the rat uterus after castration was investigated. Progesterone causes an inhibition of 3H-uridine incorporation into uterine RNA. The inhibitory effect is strongest when progesterone is administered within 1 hour before isotope labelling. Evaluation of the free labelled nucleotides shows that this effect is not achieved by a blockade of permeability at the cell membrane or by reduction of the phosphorylation process. As a result of this progesterone-induced inhibition, protein synthesis is also reduced. These results are discussed with regard to the modifying effect of progesterone on oestrogen dependent functions in the uterus.

scholarly journals Metabolic aspects of growth in HU-treated crown-gall tissue cultures. II. Helianthus annuus

2015 ◽  
Vol 46 (1) ◽  
pp. 101-118
Author(s):  
Aldona Rennert
Keyword(s):  
Protein Synthesis ◽  
Nucleic Acid ◽  
Crown Gall ◽  
Specific Activity ◽  
Tissue Growth ◽  
The Other ◽  
Tissue Cultures ◽  
Rna And Protein Synthesis ◽  
Crown Gall Tissue ◽  
Induced Changes

The dynamics of growth and changes in nucleic acid and protein contents in sunflower calluses and tumours cultured in hydroxyurea (HU) containing media were examined. HU-induced changes in healthy tissues ran in parallel always in the same direction, in tumourous ones however an uncoupling between DNA synthesis and tissue growth on one hand and RNA and protein synthesis on the other took place. A detailed analysis of the results allows to suppose that the specific activity of HU on tumourous tissue could be an index of: 1) quantitative disturbances in its genes function (2) degree of the lass of sensitivity to the factors of regulation.

scholarly journals Proliferation of Megaloblasts in Pernicious Anemia as Observed from Nucleic Acid Metabolism

Blood ◽  
1968 ◽  
Vol 31 (3) ◽  
pp. 292-303 ◽  
Author(s):  
YATARO YOSHIDA ◽  
AKIO TODO ◽  
SHIGERU SHIRAKAWA ◽  
GYOICHI WAKISAKA ◽  
HARUTO UCHINO
Keyword(s):  
Protein Synthesis ◽  
Nucleic Acid ◽  
Pernicious Anemia ◽  
Acid Metabolism ◽  
Cellular Level ◽  
The Other ◽  
Nucleic Acid Metabolism ◽  
Dna Values ◽  
Rna And Protein Synthesis ◽  
S Period

Abstract In order to elucidate the nature of the megaloblastic lesion at the cellular level, DNA, RNA, and protein synthesis was studied in megaloblasts of pernicious anemia. While microphotometric estimation of DNA content showed an increase in cells with DNA values ranging around the 4c value, autoradiographic studies with H3-thymidine indicated, rather, a decreased ability to synthesize DNA. Combined microphotometric and autoradiographic studies suggested the impaired DNA synthesis with occasional arrests of synthesis, as well as the prolongation of the S period with or without the prolongation of the G2 period. On the other hand, active incorporation of H3-uridine and H3-leucine indicated active or unaffected RNA and protein synthesis. Vitamin B12 treatment rapidly corrected these aberrant patterns of synthesis. The significance of these findings has been discussed in relation to the mechanism of megaloblastic hemopoiesis.

FEMALE ENDOCRINE CONTROL MECHANISMS DURING THE NEONATAL PERIOD

1972 ◽  
Vol 70 (2) ◽  
pp. 396-408 ◽  
Author(s):  
K.-D. Schulz ◽  
H. Haarmann ◽  
A. Harland
Keyword(s):  
Protein Synthesis ◽  
Reproductive System ◽  
Rna Synthesis ◽  
Neonatal Period ◽  
High Dose ◽  
Female Reproductive System ◽  
Endocrine Control ◽  
Hypothalamic Region ◽  
Rna And Protein Synthesis ◽  
Physiological Dose

ABSTRACT The present investigation deals with the oestrogen-sensitivity of the female reproductive system during the neonatal period. Newborn female guinea pigs were used as test animals. At different times after a single subcutaneous injection of a physiological dose of 0.1 μg or an unphysiologically high dose of 10 μg 17β-oestradiol/100 g body weight, the RNA- and protein-synthesis was examined in the hypothalamic region, pituitary, cerebral cortex, liver, adrenal gland, ovary and uterus. With a physiological dose an increase in organ weight, protein content, RNA-and protein-synthesis was found only in the uterus. These alterations turned out to be dose-dependent. In addition to the findings in the uterus an inhibition of the aminoacid incorporation rate occurred in the liver following the injection of the high oestradiol dose. As early as 1 hour after the administration of 0.1 μg 17β-oestradiol an almost 100% increase in uterine protein synthesis was detectable. This result demonstrates a high oestrogen-sensitivity of this organ during the neonatal period. All the other organs of the female reproductive system such as the hypothalamus, pituitary and ovary did not show any oestrogen response. Therefore the functional immaturity of the uterus during post partem life is not the result of a deficient hormone sensitivity but is correlated with the absence of a sufficient hormonal stimulus at this time. The investigation on the effects of actinomycin resulted in different reactions in the uterus and liver. In contrast to the liver a paradoxical actinomycin effect was found in the uterus after treatment with actinomycin alone. This effect is characterized by a small inhibition of RNA-synthesis and a 50% increase in protein synthesis. The treatment of the newborn test animals with actinomycin and 17β-oestradiol together abolished the oestrogen-induced stimulation of the uterine RNA-and protein-synthesis. Consequently, the effect of oestrogens during the neonatal period is also connected with the formation of new proteins via an increased DNA-directed RNA-synthesis.

scholarly journals RGD4C peptide mediates anti-p21Ras scFv entry into tumor cells and produces an inhibitory effect on the human colon cancer cell line SW480

BMC Cancer ◽  
2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Chen-Chen Huang ◽  
Fang-Rui Liu ◽  
Qiang Feng ◽  
Xin-Yan Pan ◽  
Shu-Ling Song ◽  
...  
Keyword(s):  
Antitumor Activity ◽  
Cell Membrane ◽  
Fusion Protein ◽  
Tumor Cell ◽  
Tumor Cells ◽  
Fusion Proteins ◽  
Inhibitory Effect ◽  
Endosomal Escape ◽  
Antitumor Effects ◽  
Sw480 Cells

Abstract Background We prepared an anti-p21Ras scFv which could specifically bind with mutant and wild-type p21Ras. However, it cannot penetrate the cell membrane, which prevents it from binding to p21Ras in the cytoplasm. Here, the RGD4C peptide was used to mediate the scFv penetration into tumor cells and produce antitumor effects. Methods RGD4C-EGFP and RGD4C-p21Ras-scFv recombinant expression plasmids were constructed to express fusion proteins in E. coli, then the fusion proteins were purified with HisPur Ni-NTA. RGD4C-EGFP was used as reporter to test the factors affecting RGD4C penetration into tumor cell. The immunoreactivity of RGD4C-p21Ras-scFv toward p21Ras was identified by ELISA and western blotting. The ability of RGD4C-p21Ras-scFv to penetrate SW480 cells and colocalization with Ras protein was detected by immunocytochemistry and immunofluorescence. The antitumor activity of the RGD4C-p21Ras-scFv was assessed with the MTT, TUNEL, colony formation and cell migration assays. Chloroquine (CQ) was used an endosomal escape enhancing agent to enhance endosomal escape of RGD4C-scFv. Results RGD4C-p21Ras-scFv fusion protein were successfully expressed and purified. We found that the RGD4C fusion protein could penetrate into tumor cells, but the tumor cell entry of was time and concentration dependent. Endocytosis inhibitors and a low temperature inhibited RGD4C fusion protein endocytosis into cells. The change of the cell membrane potential did not affect penetrability. RGD4C-p21Ras-scFv could penetrate SW480 cells, effectively inhibit the growth, proliferation and migration of SW480 cells and promote this cells apoptosis. In addition, chloroquine (CQ) could increase endosomal escape and improve antitumor activity of RGD4C-scFv in SW480 cells. Conclusion The RGD4C peptide can mediate anti-p21Ras scFv entry into SW480 cells and produce an inhibitory effect, which indicates that RGD4C-p21Ras-scFv may be a potential therapeutic antibody for the treatment of ras-driven cancers.

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