PLASMA TESTOSTERONE LEVELS FOLLOWING SURGICAL STRESS IN MALE PATIENTS

1970 ◽  
Vol 65 (1) ◽  
pp. 11-17 ◽  
Author(s):  
K. Matsumoto ◽  
K. Takeyasu ◽  
S. Mizutani ◽  
Y. Hamanaka ◽  
T. Uozumi
Keyword(s):  
Postoperative Period ◽  
Plasma Levels ◽  
Surgical Stress ◽  
Control Level ◽  
Major Surgery ◽  
Plasma Testosterone ◽  
Blood Samples ◽  
Testosterone Levels ◽  
Competitive Protein Binding ◽  
Male Patients

ABSTRACT Plasma testosterone levels were determined by the competitive protein binding method of Mayes & Nugent (1968) with slight modifications, in 20 male patients in whom moderate or major surgery had been performed. Preoperative (control) and postoperative blood samples were obtained at 8 a. m. except for immediate postoperative samples. There was a significant decrease in the plasma levels of testosterone immediately and 2 days after moderate and major surgery. The level of testosterone returned toward the control level on the sixth postoperative day after moderate surgery, while after major surgery, the decreased plasma testosterone was maintained for at least 6 days. It is suggested that testicular secretion of testosterone seems to decrease during the postoperative period.

INFLUENCE OF MAJOR SURGICAL STRESS ON PLASMA TESTOSTERONE, PLASMA LH AND URINARY STEROIDS

1972 ◽  
Vol 69 (3) ◽  
pp. 542-552 ◽  
Author(s):  
Y. Monden ◽  
K. Koshiyama ◽  
H. Tanaka ◽  
S. Mizutani ◽  
T. Aono ◽  
...  
Keyword(s):  
Marked Decrease ◽  
Surgical Stress ◽  
Major Surgery ◽  
Plasma Testosterone ◽  
Pulmonary Lobectomy ◽  
Adrenal Androgens ◽  
Testosterone Secretion ◽  
Urinary Steroids ◽  
Post Operative Period ◽  
Male Patients

ABSTRACT Plasma testosterone levels were determined in 42 male patients both before 1, 2, 3, 4, 6, 8 and 12 weeks following pulmonary lobectomy. The plasma LH was measured in 13 of the 42 patients. There was a significant fall in the plasma levels of testosterone 1, 2 and 3 weeks after the pulmonary lobectomy but the levels returned to the control values from the fourth post-operative week onward. On the other hand, the level of plasma LH increased significantly one week after the operation when the plasma testosterone showed the most marked decrease. The urinary 17-KS and 17-OHCS were measured in 27 of the 42 male patients, both before and 1, 4, 8 and 12 weeks after the operation. The total 17-KS was found to be significantly decreased 1, 4 and 8 weeks after the operation. The post-operative decrease resulted from a significant decrease in the excretion of C19O2-17-KS on the seventh postoperative day but thereafter the decrease mainly resulted from a decrease in C19O3-17-KS. It is suggested that testosterone secretion from the testes seems to decrease for 3 weeks after major surgery. The decrease in the excretion of 17-KS observed after this post-operative period is caused by a decrease in the excretion of metabolites from adrenal androgens.

EFFECTS OF GENERAL ANAESTHESIA AND SEVERITY OF SURGICAL STRESS ON SERUM LH AND TESTOSTERONE IN MALES

1975 ◽  
Vol 78 (2) ◽  
pp. 258-269 ◽  
Author(s):  
A. Nakashima ◽  
K. Koshiyama ◽  
T. Uozumi ◽  
Y. Monden ◽  
Y. Hamanaka ◽  
...  
Keyword(s):  
General Anaesthesia ◽  
Surgical Stress ◽  
Fiberoptic Bronchoscopy ◽  
Serum Testosterone ◽  
Major Surgery ◽  
Succinylcholine Chloride ◽  
Serum Luteinizing Hormone ◽  
Serum Lh ◽  
Rate Of Increase ◽  
Male Patients

ABSTRACT Significantly decreased levels of serum testosterone from the pre-anaesthesia level were found during and up to 7 days following major surgery under general anaesthesia (nitrous oxide, oxygen and halothane following induction with thiopental and succinylcholine chloride) in 18 male patients. On the other hand, in the same patients, the serum luteinizing hormone (LH) increased significantly from the pre-anaesthesia level 30 min and 1 h after the beginning of anaesthesia. A slight increase in LH level was also noted on the 7th post-operative day. The determinations of serum testosterone and LH in fiberoptic bronchoscopy under the same general anaesthesia as that used in surgery or local anaesthesia in 26 male patients, revealed that the change in the serum LH during and following surgery seemed to be mainly induced by the general anaesthesia and that the rate of decrease in the serum testosterone may be related to the severity of surgical stress including the anaesthesia. The rate of increase in serum testosterone following the injection of gonadotrophin in 20 males on the 6th post-operative day was similar to that in 10 pre-operative males. The effects of pulmonary lobectomy on serum testosterone and urinary steroids were also studied in 6 males under adrenal suppression with dexamethasone. On the 6th post-operative day, the urinary aetiocholanolone plus androsterone and serum testosterone were found to be half the level of those on the pre-operative day, while the urinary 5β-pregnane-3α,17α,20α-triol remained unchanged. These observations in human are not inconsistent with the report of Tcholakian & Eik-Nes (1971) in dogs namely that a shift in androgen biosynthetic pathway is present in the testis under surgical stress.

SEASONAL VARIATIONS IN BLOOD LUTEINIZING HORMONE AND TESTOSTERONE LEVELS IN RAMS

1974 ◽  
Vol 60 (1) ◽  
pp. 101-106 ◽  
Author(s):  
C. B. KATONGOLE ◽  
F. NAFTOLIN ◽  
R. V. SHORT
Keyword(s):  
Luteinizing Hormone ◽  
Seasonal Changes ◽  
Binding Assay ◽  
Endocrine Activity ◽  
Blood Samples ◽  
Testosterone Levels ◽  
Protein Binding Assay ◽  
Competitive Protein Binding Assay ◽  
Competitive Protein Binding ◽  
Lh Secretion

SUMMARY Three adult Suffolk rams were bled at weekly intervals for 14 months, and at hourly intervals for 24 h during the summer (one ram) and autumn (two rams). Luteinizing hormone (LH) was measured by radioimmunoassay, and testosterone by a competitive protein binding assay. There were episodic bursts of LH secretion during the 24 h period in both the summer and the autumn; the frequency of discharge was lower in the summer, but the amplitude (0·1–8·0 ng/ml) did not appear to change. It was not always possible to detect seasonal changes in LH concentration in single blood samples taken once a week throughout the year. The blood testosterone levels also showed marked fluctuations throughout the 24 h, and the frequency of the peaks was lower in the summer. But in contrast to LH, the magnitude of the testosterone peaks also changed throughout the year; from January to September the testosterone concentration ranged from 0·5 to 10 ng/ml plasma, whereas from October to December it ranged from 3 to 28 ng/ml. Thus in temperate regions the ram, like the ewe, shows seasonal changes in gonadal endocrine activity, although some degree of spermatogenesis continues throughout the whole year.

PLASMA TESTOSTERONE LEVELS IN INTACT AND HEMICASTRATED GROWING COCKERELS

1979 ◽  
Vol 81 (2) ◽  
pp. 169-174 ◽  
Author(s):  
F. J. M. DRIOT ◽  
M. DE REVIERS ◽  
J. WILLIAMS
Keyword(s):  
Plasma Testosterone ◽  
Early Age ◽  
Blood Samples ◽  
Testosterone Levels ◽  
Testosterone Secretion ◽  
Testicular Hypertrophy

Changes in the levels of testosterone in plasma were measured by radioimmunoassay in blood samples taken at frequent intervals between 2 and 26 weeks of age from entire cockerels and cockerels hemicastrated before 2 weeks of age. In both groups the pattern of testosterone secretion could be divided into three clearly defined phases. In young birds, the levels of testosterone in plasma were low (0·3 ng/ml) but in the prepubertal period, at 11 weeks of age, they started to rise and continued to rise until 22 weeks of age when adult levels, which fluctuated between 2·5 and 3·5 ng/ml, were reached. In the immediate period after hemicastration, the concentration of testosterone decreased temporarily. From 11 weeks of age the levels of testosterone in the hemicastrated birds were approximately 75% of those in intact birds. These results are discussed in relation to the compensatory testicular hypertrophy which occurs in growing cockerels hemicastrated at an early age.

Effects of Long-Term Erythropoietin Therapy on the Hypothalamo–Pituitary–Testicular Axis in Male Capd Patients

2001 ◽  
Vol 21 (5) ◽  
pp. 448-454 ◽  
Author(s):  
Bülent Tokgöz ◽  
Cengiz Utaş ◽  
Ayhan Dogukan ◽  
Muhammet Güven ◽  
Hülya Taşkapan ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Luteinizing Hormone ◽  
Healthy Volunteers ◽  
Free Testosterone ◽  
Blood Samples ◽  
Testosterone Levels ◽  
Gonadal Dysfunction ◽  
Before And After ◽  
Male Patients ◽  
Lh Rh

Objective Gonadal dysfunction has been recognized for a long time in uremic male patients. The present study assesses the hypothalamo–pituitary–testicular axis and growth hormone status in male continuous ambulatory peritoneal dialysis (CAPD) patients, before and after recombinant human erythropoietin (rHuEPO) therapy. Design Single-center prospective study. Subjects Ten anemic male patients with chronic renal insufficiency, and 11 healthy volunteers with normal renal function, matched for age, were included in the study. All patients were on CAPD therapy and none had received rHuEPO treatment previously. Main Outcome Measures Blood samples were collected between 0800 and 0900 hr from all patients for the determination of basal follicle stimulating hormone (FSH), luteinizing hormone (LH), and growth hormone (GH) levels. A luteinizing hormone-releasing hormone (LH-RH) stimulation test was carried out using LH-RH 100 mg intravenous as a bolus injection. Blood for FSH, LH, and GH determinations was drawn every 30 minutes during the 3-hour test period. Human chorionic gonadotropin (hCG) test was performed after 48 hours. After estimations of basal serum total and free testosterone levels, 2000 IU hCG was administered intramuscularly and repeated 48 hours later. Total and free testosterone levels were measured in blood samples collected before and 48 hours after two injections of hCG. After improvement in anemia with exogenous rHuEPO, LH-RH and hCG tests were repeated. Results Baseline FSH concentrations before and after rHuEPO treatment were slightly higher in CAPD patients than in healthy volunteers ( p = 0.85 and p = 0.70, respectively). Areas-under-the-curve (AUCs) for FSH secretion before and after rHuEPO treatment were also slightly higher in patients than in healthy volunteers ( p = 1.00 and p = 0.75, respectively). The pretreatment basal LH levels in patients were significantly higher than in controls ( p < 0.001). After the improvement in anemia with rHuEPO, serum LH levels declined significantly ( p < 0.05). The AUCs for LH secretion before and after rHuEPO treatment were significantly higher in patients than in controls ( p < 0.05). All patients had elevated basal levels of GH with paradoxical response to LH-RH. Baseline GH levels in patients were significantly higher than those in healthy subjects ( p < 0.001) before rHuEPO treatment. After treatment with rHuEPO, basal GH levels declined but did not normalize, and baseline levels of free testosterone increased significantly ( p < 0.05). Conclusion Anemic uremic male patients on CAPD have normal levels of testosterone with normal response to hCG administration, elevated basal levels of GH, and elevated basal levels of LH, with exaggerated response to LH-RH administration. Improvement in anemia with rHuEPO reduced the basal levels of LH and GH, but exaggerated the LH response; paradoxical GH response to LH-RH administration persisted. These results indicate a defect at the level of the hypothalamus and pituitary gland in uremic male patients undergoing CAPD, and that the improvement in anemia with rHuEPO partially restores some of these endocrine abnormalities.

PLASMA LEVELS OF TESTOSTERONE IN BULLS

1978 ◽  
Vol 88 (4) ◽  
pp. 787-792 ◽  
Author(s):  
Anne Sundby ◽  
P. A. Torjesen
Keyword(s):  
Testosterone Level ◽  
Plasma Levels ◽  
Leydig Cells ◽  
Sharp Decrease ◽  
Plasma Testosterone ◽  
Plasma Samples ◽  
Elevated Plasma ◽  
Plasma Testosterone Level ◽  
Testosterone Levels ◽  
Testosterone Production

ABSTRACT Administration of 6000 IU HCG to 4 bulls was followed by an elevation of plasma testosterone lasting for 9–13 days. When HCG administration was repeated, the testosterone response was shortened to 4–6 days in 3 bulls due to the formation of antibodies against HCG. The appearance of HCG antibodies coincided with a sharp decrease in the plasma testosterone level, indicating that Leydig cells have to be under continuous HCG stimulation to maintain increased testosterone production. No antibody against bovine LH was detected in the plasma samples containing antibodies against HCG. In one bull the response following the second HCG injection was similar to the plasma testosterone pattern following the first. No antibodies against HCG were found in this bull. Five bulls received 750 IU HCG twice. Following the period with elevated plasma testosterone levels, subnormal levels were observed after both injections. One injection led to decreased levels without development of antibodies against HCG while the second HCG injection led to subnormal testosterone levels concomitant with measurable antibodies against HCG.

EFFECTS OF AN INTRAVENOUS INJECTION OF TETRACOSACTID ON PLASMA CORTICOSTEROID AND TESTOSTERONE LEVELS IN UNSTRESSED MALE RABBITS

1979 ◽  
Vol 91 (3) ◽  
pp. 511-518 ◽  
Author(s):  
K. W. Faulborn ◽  
M. Fenske ◽  
L. Pitzel ◽  
A. König
Keyword(s):  
Body Weight ◽  
Intravenous Injection ◽  
Plasma Levels ◽  
Plasma Testosterone ◽  
Rapid Rise ◽  
Testosterone Concentration ◽  
Testosterone Levels ◽  
Blood Withdrawal ◽  
Basal Plasma ◽  
Pre Treatment

ABSTRACT Administration of tetracosactid into male rabbits, fitted with permanently indwelling jugular catheters, resulted in a rapid rise of plasma corticosteroids and plasma testosterone. Corticosteroid concentrations were significantly elevated at 40 and 60 min and testosterone concentrations 20 min after the iv injection of tetracosactid (2.5, 5.0, and 10.0 μg/kg body weight), in comparison to pre-treatment levels. Corticosteroid values in plasma were elevated as long as 120 min after tetracosactid injection. In contrast, testosterone levels were lower at 60–120 min after tetracosactid injection than corresponding pre-treatment values. However, these differences were not significant. At the doses used no tetracosactiddose-dependent corticosteroid or testosterone release could be found; apparently, testosterone release is only dependent upon basal plasma levels but not upon the dose of tetracosactid applied. From these studies it is concluded that tetracosactid may bring about an increase or decrease of testosterone concentration in plasma in the buck depending upon the length of time elapsing between injection of tetracosactid and blood withdrawal.

Cycles annuels de la testostéronémie et de la thyroxinémie chez Vipera berus L. (Reptilia: Viperidae) en relation avec le cycle sexuel et l'hivernage

1988 ◽  
Vol 9 (1) ◽  
pp. 33-42 ◽  
Author(s):  
François Fleury ◽  
Guy Naulleau
Keyword(s):  
Seasonal Variations ◽  
Secondary Peak ◽  
Plasma Testosterone ◽  
Maximal Concentration ◽  
Mating Period ◽  
Blood Samples ◽  
Vipera Berus ◽  
Testosterone Levels

AbstractBlood samples were taken in Vipera berus during a five year period. Changes in plasma thyroxine and testosterone levels were examined. Plasma testosterone levels show a marked profile. Highest levels are observed a few weeks after the emergence of hibernation from the end of March till the end of April. These values coincide with the mating period and spring spermiogenesis. Plasma thyroxine levels show also seasonal variations. Low values are observed in winter. Plasma thyroxine levels increase in spring and reach a maximal concentration shortly after the peak of plasma testosterone. A secondary peak is observed at the end of summer.

PERIPHERAL PLASMA LEVELS OF 17α-HYDROXYPROGESTERONE, PROGESTERONE AND OESTRADIOL DURING NORMAL MENSTRUAL CYCLES IN WOMEN

1972 ◽  
Vol 71 (4) ◽  
pp. 743-754 ◽  
Author(s):  
Tore H:son Holmdahl ◽  
Elof D. B. Johansson
Keyword(s):  
Menstrual Cycle ◽  
Plasma Levels ◽  
Luteal Phase ◽  
Cycle Length ◽  
Gel Chromatography ◽  
Blood Samples ◽  
Menstrual Cycles ◽  
Peripheral Plasma ◽  
Competitive Protein Binding ◽  
Human Menstrual Cycle

ABSTRACT Liquid-gel chromatography on hydroxyalkoxypropyl Sephadex has been used to separate 17α-hydroxyprogesterone* and progesterone from interfering steroids prior to assay by competitive protein binding. During the luteal phase 0.5 ml of plasma was enough for determinations of both steroids. Fifteen samples could be assayed in less than 48 h. Oestradiol in plasma was assayed by radioimmunoassay. Daily blood samples were collected during 10 normal menstrual cycles in young, healthy women. The average cycle length was 29.7±2.0 (sd) days. 17α-hydroxyprogesterone displayed a midcyclic peak averaging 1.86±0.70 (sd) ng per ml coinciding with a midcyclic peak of oestradiol averaging 16.50± 5.95 (sd) ng per 100 ml of plasma. The highest luteal phase level of 17α-hydroxyprogesterone was 1.94 ±0.72 (sd) ng per ml. The corresponding levels for oestradiol were 9.1 ±3.8 (sd) ng per 100 ml. Progesterone formed a luteal plateau averaging 12.3±2.3 (sd) days. The highest luteal level of progesterone was 14.6±2.1 (sd) ng per ml. The peripheral plasma pattern of 17α-hydroxyprogesterone seems to mimic that of oestradiol during the human menstrual cycle.

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