SUSTAINED RELEASE HORMONAL PREPARATIONS

1970 ◽  
Vol 63 (1) ◽  
pp. 29-38 ◽  
Author(s):  
G. Benagiano ◽  
M. Ermini ◽  
C. C. Chang ◽  
K. Sundaram ◽  
Fred A. Kind
Keyword(s):  
Sustained Release ◽  
Radioactive Material ◽  
Diffusion Rates

ABSTRACT Determination of radioactivity in urine and faeces was used to monitor absorption of labelled 6-methyl-17α-acetoxypregna-4,6-diene-3,20-dione from polydimethylsiloxane implants. Based on previously determined in vitro diffusion rates, mean absorption was 60% in hamsters, 80% in rabbits, and 85% in rats during the first month after implantation. Thereafter, excretion of radioactive material has slowly decreased. After six months, the rate declined to 40% of the original value.

scholarly journals Study of Release-Retardant Polymers in Formulation Development of Cinnarizine-HCl Sustained-release Oral Suspension Using Raft Technology

2020 ◽  
Vol 19 (1) ◽  
pp. 14-24
Author(s):  
Tabinda Islam ◽  
Nusrat Hossain ◽  
Mohsina Rahman ◽  
Sadia Shabnam ◽  
Eyasmin Chowdhury ◽  
...  
Keyword(s):  
Sustained Release ◽  
In Vitro Dissolution ◽  
Formulation Development ◽  
Hydrochloric Acid Medium ◽  
Sodium Saccharin ◽  
Physical Mixing ◽  
Mixing Method ◽  
Usp Apparatus

The main objective of this research was to develop a sustained-release suspension of cinnarizine hydrochloride using raft-forming technique. This innovative approach has been utilized to formulate a series of suspension formulations using hydroxypropyl cellulose (HPC) as a release-retardant polymeric agent. Cinnarizine sustained-release suspensions were prepared by physical mixing method with varying concentrations and combinations of HPC, sodium citrate, sodium saccharin, calcium carbonate, sodium alginate, methyl hydroxybenzoate and propyl hydroxybenzoate. The formulations were subjected for determination of floating time, floating lag time, weight of the raft, physical appearance and in-vitro dissolution. The dissolution was conducted through USP apparatus 2 (paddle type) in 0.1N hydrochloric acid medium having pH 1.2. The key findings of the study demonstrate that a stable sustained-release suspension of cinnarizine can be formulated using raft-forming approach for increased bioavailability and patient-convenience. Dhaka Univ. J. Pharm. Sci. 19(1): 15-24, 2020 (June)

Determination of in vitro „anti-aging„-effects of Ganoderma pfeifferi

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
W Jülich ◽  
J Pörksen ◽  
H Welzel ◽  
U Lindequist
Keyword(s):  
Aging Effects

In vitro determination of the skin anti-aging potential of eleven South African plants

Planta Medica ◽  
2013 ◽  
Vol 79 (13) ◽  
Author(s):  
GN Ndlovu ◽  
G Fouche ◽  
W Cordier ◽  
V Steenkamp ◽  
M Tselanyane
Keyword(s):  
South African ◽  
South African Plants ◽  
African Plants

A Molecular Approach to Immunoscintigraphy: A Study of the T-Antigen Conformation on the Surface of Tumors

1987 ◽  
Vol 26 (01) ◽  
pp. 1-6 ◽  
Author(s):  
S. Selvaraj ◽  
M. R. Suresh ◽  
G. McLean ◽  
D. Willans ◽  
C. Turner ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Tumor Cell ◽  
T Antigen ◽  
Human Carcinomas ◽  
Animal Tumor ◽  
Synthetic Antigens

The role of glycoconjugates in tumor cell differentiation has been well documented. We have examined the expression of the two anomers of the Thomsen-Friedenreich antigen on the surface of human, canine and murine tumor cell membranes both in vitro and in vivo. This has been accomplished through the synthesis of the disaccharide terminal residues in both a and ß configuration. Both entities were used to generate murine monoclonal antibodies which recognized the carbohydrate determinants. The determination of fine specificities of these antibodies was effected by means of cellular uptake, immunohistopathology and immunoscintigraphy. Examination of pathological specimens of human and canine tumor tissue indicated that the expressed antigen was in the β configuration. More than 89% of all human carcinomas tested expressed the antigen in the above anomeric form. The combination of synthetic antigens and monoclonal antibodies raised specifically against them provide us with invaluable tools for the study of tumor marker expression in humans and their respective animal tumor models.

Storage of Human Blood Platelets

1974 ◽  
Vol 32 (02/03) ◽  
pp. 405-416 ◽  
Author(s):  
M. R Hardeman ◽  
Carina J L. Heynens
Keyword(s):  
Storage Temperature ◽  
Platelet Rich Plasma ◽  
Blood Platelets ◽  
Storage Period ◽  
Serotonin Uptake ◽  
Hypotonic Shock ◽  
Glycolytic Intermediates

SummaryStorage experiments were performed at 4°, 25° and 37° C with platelet-rich plasma under sterile conditions. In some experiments also the effect of storing platelets at 4° C in whole blood was investigated.Before, during and after three days of storage, the platelets were tested at 37° C for their serotonin uptake and response to hypotonic shock. In addition some glycolytic intermediates were determined.A fair correlation was noticed between the serotonin uptake and hypotonic shock experiments. Both parameters were best maintained at 25° C. Also platelet counting, performed after the storage period, indicated 25° C as the best storage temperature. Determination of glycolytic intermediates did not justify any conclusion regarding the optimal storage temperature. Of the various anticoagulants studied, ACD and heparin gave the best results as to the serotonin uptake and hypotonic shock response, either with fresh or stored platelets. The use of EDTA resulted in the lowest activity, especially after storage.The results of these storage experiments in vitro, correspond well with those in vivo reported in the literature.

Studies on Metabolism of Urokinase and Mechanism of Thrombolysis by Urokinase

1979 ◽  
Vol 42 (03) ◽  
pp. 885-894 ◽  
Author(s):  
Tatsuo Ueno ◽  
Norio Kobayashi ◽  
Tadashi Maekawa
Keyword(s):  
Molecular Weight ◽  
Plasma Clearance ◽  
Radioactive Material ◽  
Optimal Dosage ◽  
Plasma Clot ◽  
Optimal Dosage Regimen ◽  
Arterial Thrombus ◽  
Contact Experiment

SummaryPharmacokinetics of intravenously injected 125I-labeled urokinase (125I-UK) of a molecular weight of 33,000 daltons in normal rabbits and patients with various diseases were investigated. The plasma clearance of 125I-UK in rabbits was described by a biexponential curve within six hours with a half-life of 8 minutes, 2.3 hours, respectively. The radioactivity in the liver and kidneys 15 minutes after iv injection with 125I-UK was 9.6% and 14.0% of the radioactivity injected, respectively. Approximately 80% of the total radioactive material injected was excreted in the urine in 18 hours. No increase in activator activity in the urine was observed after a large amount of UK injection. Activity uptake of 125I-UK by experimentally induced arterial thrombus was little. Lysis of the stasis thrombus was produced by injecting 7.5 × 104 IU of UK in only one out of 8 rabbits. In vitro contact experiment revealed that transfer of 125I-UK to plasma clot is slow (24 hours for 10% of 125I-UK by plasma clot). In 4 patients plasma clearance of 125I-UK was essentially similar to that in rabbits. From the results obtained optimal dosage regimen of UK administration for complete thrombolysis in vivo was discussed.

DETERMINATION OF BLOOD CORTICOIDS USING THE IN VITRO RESIN UPTAKE OF 3H-PREDNISOLONE

1968 ◽  
Vol 57 (1) ◽  
pp. 23-32 ◽  
Author(s):  
Hironori Nakajima ◽  
Mitsunori Murala ◽  
Masumitsu Nakata ◽  
Takeshi Naruse ◽  
Seiji Kubo
Keyword(s):  
Thyroid Function Test ◽  
Normal Subjects ◽  
Adrenal Function ◽  
Function Test ◽  
Before And After ◽  
Adrenal Response ◽  
Suppression Test

ABSTRACT The in vitro resin uptake of 3H-prednisolone was used for the determination of blood cortisol after addition of radioactive prednisolone followed by Amberlite CG 400 Type 1 to the test serum, and incubation of the mixture. The radioactivity of the supernatant was compared before and after the addition of the resin. The principle of this method is similar to that of the 131I-triiodothyronine resin uptake for the thyroid function test. The tests for the specificity, reproducibility and sensitivity gave satisfactory results. The mean basal value ± SD of the 3H-prednisolone resin uptake was 35.3 ± 9.2% in normal subjects, and 27.1 ± 4.8% in pregnant women. This method was valid in various adrenal function tests, i. e. the adrenal circadian rhythm, corticotrophin (ACTH) test, dexamethasone suppression test and the adrenal response to lysine-8-vasopressin. It proved to be a sensitive indicator of the adrenal function. These results suggest that this method should be useful for a routine adrenal function test.

HPTLC determination of catechins in in-vitro cultures of two species of the genusPhyllanthus

2008 ◽  
Vol 21 (2) ◽  
pp. 103-106 ◽  
Author(s):  
Barbara Sparzak ◽  
Mirosława Krauze-Baranowska ◽  
Loretta Pobłocka-Olech
Keyword(s):  
In Vitro Cultures
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