STUDIES ON HUMAN CHORIONIC GONADOTROPHIN

1968 ◽  
Vol 59 (1) ◽  
pp. 105-119 ◽  
Author(s):  
B. C. Goverde ◽  
F. J. N. Veenkamp ◽  
J. D. H. Homan
Keyword(s):  
Sialic Acid ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Chemical Assay ◽  
Mathematical Relation ◽  
Sharp Band ◽  
Biological Potency ◽  
Independent Variable ◽  
The One

ABSTRACT Six preparations of Human Chorionic Gonadotrophin (HCG), differing in purity and biological potency, were analysed for their carbohydrate and amino acid composition. The results of the determinations indicated a certain relation between the biological potency on the one hand, and the content of certain substances, particularly sialic acid and fucose (both carbohydrate terminal groups of glycoproteins), on the other. As the sialic acid fraction contained only N-acetyl-neuraminic acid (NANA), a true quantitative determination could be made. Two methods used for the release of NANA were compared and it was found that neuraminidase from Vibrio cholerae released 96% of the amount of NANA which could be released by 0.01 n hydrochloric acid hydrolysis. Based on the results of biological and NANA assays of 16 preparations, a mathematical relation between the biological potency and the NANA content, as an independent variable, was calculated. Membrane electrophoresis in Veronal-formamide buffer revealed a sharp band pattern, which could be explained by assuming a polymorphous HCG molecule, differing mainly in the number of terminal NANA groups. The prospects of a chemical assay of HCG based on the determination of NANA are, however, not at all encouraging.

STUDIES ON HUMAN CHORIONIC GONADOTROPHIN

1968 ◽  
Vol 59 (1) ◽  
pp. 89-104 ◽  
Author(s):  
H. van Hell ◽  
R. Matthijsen ◽  
J. D. H. Homan
Keyword(s):  
Sialic Acid ◽  
Calcium Ions ◽  
Gel Electrophoresis ◽  
Gel Filtration ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Starch Gel Electrophoresis ◽  
Sialic Acid Content ◽  
Starch Gel ◽  
Biological Potency

ABSTRACT Highly purified preparations of Human Chorionic Gonadotrophin (HCG), with potencies up to 18 800 IU/mg, were obtained from material containing about 1500 IU/mg, by using a fractionation with ethanol, CMSephadex chromatography and gel filtration with Sephadex G-100. Starch gel electrophoresis revealed that the most potent preparation still contained three components, while certain other fractions with much lower potencies were found to be homogeneous. Evidence is presented for the presence of various HCG components differing from each other in biological potency, electrophoretic mobility and sialic acid content. Within this material, components with higher electroporetic mobilities and higher sialic acid contents showed higher biological potencies. The molecular weight estimated in the absence of calcium ions was 62 000 ± 1000.

DIFFERENCES IN THE BIOLOGICAL AND IMMUNOLOGICAL ACTIVITIES OF HUMAN CHORIONIC GONADOTROPHIN AFTER THE REMOVAL OF SIALIC ACID BY ENZYMIC HYDROLYSIS

1967 ◽  
Vol 38 (4) ◽  
pp. 395-399 ◽  
Author(s):  
W. A. BARR ◽  
J. G. COLLEE
Keyword(s):  
Biological Activity ◽  
Sialic Acid ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Enzymic Hydrolysis ◽  
Immunological Activity ◽  
Parallel Reduction ◽  
Free Sialic Acid

SUMMARY Incubation of a partially purified preparation of human chorionic gonadotrophin (HCG) with neuraminidase resulted in release of free sialic acid from the substrate. The biological activity of the HCG was considerably reduced after incubation with the enzyme, but a parallel reduction in immunological activity was not observed.

IMMUNOLOGICAL DETERMINATION OF PITUITARY LUTEINIZING HORMONE IN THE URINE OF FERTILE AND POST-MENOPAUSAL WOMEN AND ADULT MEN

1962 ◽  
Vol 39 (4) ◽  
pp. 539-546 ◽  
Author(s):  
Leif Wide ◽  
Carl Gemzell
Keyword(s):  
Luteinizing Hormone ◽  
Haemagglutination Inhibition ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Human Pituitary ◽  
Adult Men ◽  
Menopausal Women ◽  
Post Menopausal ◽  
Inhibition Reaction

ABSTRACT An immunological method to assay human pituitary luteinizing hormone (HPLH) in urine is described. It is based on the fact that HPLH crossreacts with human chorionic gonadotrophin (HCG) in an haemagglutination inhibition reaction between HCG-coated blood cells and rabbit HCG-antisera. During the menstrual cycle the excretion of HPLH reached a peak of 200–400 U per liter at the time of ovulation. In the urine of post-menopausal women the concentration of HPLH was between 100 and 400 U per liter. In the urine of adult men the concentration of HPLH was between 50 and 160 U per liter.

An Immunonanosilver Resonance Rayleigh Scattering Spectral Probe for Rapid Assay of Human Chorionic Gonadotrophin

2013 ◽  
Vol 680 ◽  
pp. 141-144 ◽  
Author(s):  
Qing Ye Liu ◽  
Gui Qing Wen ◽  
Kun Li ◽  
Ai Hui Liang
Keyword(s):  
Citric Acid ◽  
Optimal Condition ◽  
Rayleigh Scattering ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Resonance Rayleigh Scattering ◽  
Serum Samples ◽  
Buffer Solution ◽  
Citric Acid Buffer

In pH 6.6 Na2HPO4- citric acid buffer solution and in the presence of KCl, the immunoreaction between hCG and nanosilver-labeled anti-hCG took place, the immunonanosilver-complex was formed and deposited, caused the resonance Rayleigh scattering (RRS) intensity at 510 nm decreased. In the optimal condition, the decreased RRS intensity responds linearly with the concentration of hCG over 0.125-1.75 µg/mL. Based on this, a new and simple RRS method has been proposed for the determination of hCG in serum samples, with satisfactory results.

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