STEROID HORMONE FORMATION BY THE RAT OVARY.

1967 ◽  
Vol 54 (3) ◽  
pp. 568-576 ◽  
Author(s):  
Bernard F. Rice ◽  
Albert Segaloff
Keyword(s):  
Ovarian Neoplasms ◽  
Steroid Hormone ◽  
Ovarian Neoplasm ◽  
Female Rats ◽  
Granulosa Cell Tumour ◽  
Dilution Technique ◽  
Hormone Synthesis ◽  
Ovarian Tumours ◽  
Rat Ovary ◽  
Hormone Formation

ABSTRACT Ovaries were transplanted to the spleens of spayed female rats. After 18 months, ovarian neoplasms had developed. Slices of two different ovarian tumours were incubated with 100 μc acetate-1-14C. Radioactive steroid hormone formation was assessed quantitatively by the reverse isotope dilution technique. Tissue from one ovarian neoplasm, considered to be a luteoma, formed radioactive progesterone and 20α-hydroxy-pregn-4-en-3-one. The formation of 3β-hydroxy-pregn-5-en-20-one, androst-4-ene-3,17-dione, testosterone, 3β-hydroxy-androst-5-en-17-one, 17-hydroxy-pregn-4-ene-3,20-dione, oestrone and 17β-oestradiol could not be established. No radioactive steroid hormone synthesis could be detected using granulosa cell tumour tissue.

STEROID HORMONE FORMATION BY THE RAT OVARY.

1966 ◽  
Vol 51 (1) ◽  
pp. 131-139 ◽  
Author(s):  
Bernard F. Rice ◽  
Albert Segaloff
Keyword(s):  
Steroid Hormone ◽  
Ovarian Tissue ◽  
Female Rats ◽  
Male Rats ◽  
Corpora Lutea ◽  
Castrate Male ◽  
Rat Ovary ◽  
17 Hydroxyprogesterone ◽  
Hormone Formation

ABSTRACT Ovaries were transplanted to the spleens of castrate male rats. After 120 days, slices of ovarian tissue, composed predominantly of corpora lutea, were incubated in Krebs-Ringer bicarbonate medium containing 50 μc acetate-1-14C. Radioactive steroid formation was assessed quantitatively by reverse isotope dilution. The formation of radioactive progesterone and 20α-hydroxy-pregn-4-en-3-one was established. The formation of radioactive 3β-hydroxy-pregn-5-en-20-one, androst-4-ene-3,17-dione, 17-hydroxyprogesterone, testosterone, oestrone and 17β-oestradiol could not be established. It appears that the corpus luteum of the rat, induced by endogenous gonadotrophins, forms only progestins from acetate-1-14C. Contrary to results previously obtained with ovarian tissue transplanted to female rats, radioactive steroid formation in vitro appeared to be augmented by luteinizing hormone (NIH-LH-S1) added to the incubation flasks. Administration of human chorionic gonadotrophin (200 IU/day) for 5 days prior to autopsy did not enhance acetate-1-14C incorporation in vitro.

PENTOSE PHOSPHATE PATHWAY AND STEROIDOGENESIS IN THE IMMATURE RAT OVARY AFTER MALONATE TREATMENT

1972 ◽  
Vol 70 (4) ◽  
pp. 758-766 ◽  
Author(s):  
Sudhansu K. Dey ◽  
Jayasree Sen Gupta ◽  
Sulekha Ghosh ◽  
C. Deb
Keyword(s):  
Pentose Phosphate Pathway ◽  
Steroid Hormone ◽  
Succinic Dehydrogenase ◽  
Hydroxysteroid Dehydrogenase ◽  
Pentose Phosphate ◽  
Immature Rat ◽  
Hormone Synthesis ◽  
Rat Ovary ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Stimulation Of

ABSTRACT Suppression of succinic dehydrogenase (SDH) activity resulted in stimulation of glucose-6-phosphate dehydrogenase (G-6-PD) and Δ5-3β-hydroxysteroid dehydrogenase (Δ5-3β-OHD) activities in the immature rat ovary after malonate treatment. The same treatment also produced depletion in the ovarian ascorbic acid and elevation in cholesterol concentrations, together with increase in the ovarian and uterine weight. The results indicate that stimulation of ovarian steroidogenesis, resulting from accelerated pentose phosphate pathway in combination with increased concentration of cholesterol in the gland, is possibly due to a direct effect of malonate on the immature rat ovary. The rise in ovarian and uterine weights is not due to the decreased inactivation of oestrogen in the liver, but rather to stimulation of steroid hormone synthesis in the immature ovary following malonate administration.

miR-130a-3p regulates steroid hormone synthesis in goat ovarian granulosa cells by targeting the PMEPA1 gene

2021 ◽  
Vol 165 ◽  
pp. 92-98
Author(s):  
Lu Zhu ◽  
Jing Jing ◽  
Shuaiqi Qin ◽  
Qi Zheng ◽  
Jiani Lu ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
Steroid Hormone ◽  
Hormone Synthesis ◽  
Ovarian Granulosa Cells

scholarly journals Effects of ChREBP deficiency on adrenal lipogenesis and steroidogenesis

2021 ◽  
Author(s):  
Ken Takao ◽  
Katsumi Iizuka ◽  
Yanyan Liu ◽  
Teruaki Sakurai ◽  
Sodai Kubota ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Target Genes ◽  
Adrenal Glands ◽  
Plasma Cholesterol ◽  
Steroid Hormone ◽  
Acid Synthesis ◽  
Cholesterol Content ◽  
Hormone Synthesis ◽  
Corticosterone Secretion ◽  
Element Binding Protein

Carbohydrate response element binding protein (ChREBP) is critical in the regulation of fatty acid and triglyceride synthesis in the liver. Interestingly, Chrebp-/- mice show reduced levels of plasma cholesterol, which is critical for steroid hormone synthesis in adrenal glands. Furthermore, Chrebp mRNA expression was previously reported in human adrenal glands. Thus, it remains to be investigated whether ChREBP plays a role directly or indirectly in steroid hormone synthesis and release in adrenal glands. In the present study, we find that Chrebp mRNA is expressed in mouse adrenal glands and that ChREBP binds to carbohydrate response elements. Histological analysis of Chrebp-/- mice shows no adrenal hyperplasia and less oil red O staining compared with that in wild-type mice. In adrenal glands of Chrebp-/- mice, expression of Fasn and Scd1, two enzymes critical for fatty acid synthesis, was substantially lower and triglyceride content was reduced. Expression of Srebf2, a key transcription factor controlling synthesis and uptake of cholesterol and the target genes was upregulated, while cholesterol content was not significantly altered in the adrenal glands of Chrebp-/- mice. Adrenal corticosterone content and plasma adrenocorticotropic hormone and corticosterone levels were not significantly altered in Chrebp-/- mice. Consistently, expression of genes related to steroid hormone synthesis was not altered. Corticosterone secretion in response to two different stimuli, namely 24-h starvation and cosyntropin administration, were also not altered in Chrebp-/- mice. Taking these results together, corticosterone synthesis and release were not affected in Chrebp-/- mice despite reduced plasma cholesterol levels.

scholarly journals STUDY OF CLINICOPATHOLOGICAL SPECTRUM OF OVARIAN NEOPLASMS WITH SPECIAL REFERENCE TO EXPRESSION OF ER AND PR IN MALIGNANT LESIONS

2020 ◽  
pp. 67-69
Author(s):  
Sujata Ganguli ◽  
Keya Basu ◽  
Ranjana Bandyopadhyay
Keyword(s):  
Germ Cell ◽  
Special Reference ◽  
Reproductive System ◽  
Ovarian Neoplasms ◽  
Germ Cell Tumours ◽  
Female Reproductive System ◽  
Gynaecological Malignancy ◽  
Ovarian Tumours ◽  
Epithelial Tumours ◽  
Malignant Lesions

Ovarian carcinoma is the second most common carcinoma of the female reproductive system .It is the leading cause of death from gynaecological malignancy .The purpose of this study is to observe the clinicopathological spectrum of ovarian neoplasms with the expression of ER and PR in the different malignant lesions .A total of 156 cases of ovarian tumours were included in this study, and surface epithelial tumours were found to be the commonest neoplasm followed by germ cell tumours. Expression of ER and PR was found to be highest in Serous and Endometrioid tumours. PR Negative cases showed a higher grade and stage.

Effect of nutrition on plasma lipid profile and mRNA levels of ovarian genes involved in steroid hormone synthesis in Hu sheep during luteal phase1

2013 ◽  
Vol 91 (11) ◽  
pp. 5229-5239 ◽  
Author(s):  
S. J. Ying ◽  
S. H. Xiao ◽  
C. L. Wang ◽  
B. S. Zhong ◽  
G. M. Zhang ◽  
...  
Keyword(s):  
Lipid Profile ◽  
Steroid Hormone ◽  
Plasma Lipid ◽  
Mrna Levels ◽  
Plasma Lipid Profile ◽  
Hormone Synthesis ◽  
Hu Sheep
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