FURTHER STUDIES ON THE INFLUENCE OF OESTROGENS ON ANDROGEN DEPENDENT FRUCTOSE FORMATION IN SEX ACCESSORY ORGANS

1966 ◽  
Vol 53 (3) ◽  
pp. 455-461 ◽  
Author(s):  
John A. Thomas ◽  
Edward T. Knych
Keyword(s):  
Combined Treatment ◽  
Seminal Vesicles ◽  
Synergistic Action ◽  
Initial Injection ◽  
Oestradiol Benzoate ◽  
Anterior Prostate

ABSTRACT Testosterone alone had a stimulating effect on the fructose formation in castrate mice, but the combined treatment of testosterone and oestrogen caused significant reductions in anterior prostate levels of this carbohydrate. Oestrogens were more effective in counteracting the action of testosterone when injected early rather than late after castration. Ethynyl oestradiol and oestradiol benzoate were more effective in counteracting testosterone than other oestrogens studied. There was a greater reduction in fructose levels when lower doses of injected testosterone were simultaneously administered with oestrogen(s). In the seminal vesicles a synergistic action between testosterone and various oestrogens on fructose levels was commonly observed, though antagonism is also evident. Increasing the period of time between castration and initial injection appeared to enhance the synergistic actions of the two hormones.

scholarly journals INDUCTION OF ENDOMETRIAL PEROXIDASE SYNTHESIS AND SECRETION BY ESTROGEN AND ESTROGEN ANTAGONIST

1974 ◽  
Vol 62 (2) ◽  
pp. 449-459 ◽  
Author(s):  
Andrew Churg ◽  
Winston A. Anderson
Keyword(s):  
Endoplasmic Reticulum ◽  
Golgi Apparatus ◽  
Peroxidase Activity ◽  
Secretory Granules ◽  
Combined Treatment ◽  
Initial Injection ◽  
Immature Rats ◽  
Glandular Cells ◽  
Endogenous Peroxidase ◽  
Estrogen Antagonist

Synthesis of peroxidase was induced in the uterine epithelium of immature rats by multiple doses over a 24–96-h period of either 17 ß-estradiol, the estrogen-antagonist Parke-Davis CI-628, or a combination of estradiol plus antagonist. Endogenous peroxidase activity first appeared in the cisternae of the rough endoplasmic reticulum of surface epithelial and glandular cells within 24–48 after the initial injection. Uterine peroxidase activity was also visible in the cisternae of the Golgi apparatus, in Golgi-derived secretory granules, and within the uterine and glandular lumen. Some cells of the epithelium produced little or no peroxidase, even after 96 h. Whereas the antagonist appeared to induce synthesis and secretion of peroxidase, neither the antagonist alone nor the combined treatment (estradiol plus antagonist) reproduced the estradiol-mediated growth in organ size and increased lumen diameter.

RESTORATION BY OESTRADIOL BENZOATE OF A NEURAL AND HORMONAL RHYTHM IN THE OVARIECTOMIZED RAT

1975 ◽  
Vol 64 (1) ◽  
pp. 27-35 ◽  
Author(s):  
F. R. BURNET ◽  
P. C. B. MACKINNON
Keyword(s):  
Single Injection ◽  
Time Of Day ◽  
Ovariectomized Rats ◽  
Female Rat ◽  
Functional Link ◽  
Initial Injection ◽  
Oestradiol Benzoate ◽  
Before And After ◽  
Lh Release ◽  
The Brain

SUMMARY The rate of [35S]methionine incorporation into protein in discrete cerebral areas was measured before and after the administration of oestradiol benzoate (OB) to chronically ovariectomized rats. The circadian rhythm of incorporation which is normally seen in the intact cyclic female rat was deleted by ovariectomy. A daily rhythm of incorporation reappeared, however, in all the brain areas studied 30 h after a single injection of OB (20 μg), and was still present 12 days later. The release of luteinizing hormone (LH) after administration of 20 μg OB was measured in chronically ovariectomized animals and was found to be biphasic. High levels of LH after ovariectomy were initially reduced by negative feedback, but this phase was followed 52 h later by a facilitation of LH release between 15.00 and 18.00 h. The facilitation of LH release at this time of day was still detectable 12 days after the initial injection. The evidence for a functional link between the rhythm of neural activity which is reflected by [35S]methionine incorporation, and the ability to 'time' the facilitation of LH release is discussed.

STUDIES ON EOSINOPHIL GRANULOCYTES.

1967 ◽  
Vol 56 (2) ◽  
pp. 221-224 ◽  
Author(s):  
A. P. Baker ◽  
F. Bergman ◽  
B. Josefsson ◽  
K. G. Paul
Keyword(s):  
Mast Cells ◽  
Adult Male ◽  
Rapid Growth ◽  
Levator Ani ◽  
Seminal Vesicles ◽  
Male Rats ◽  
Levator Ani Muscle ◽  
Eosinophil Granulocytes ◽  
Anterior Prostate ◽  
Long Acting

ABSTRACT Castrated, adult male rats were given a long-acting androgen in doses that caused a rapid growth of the anterior prostate lobes, the seminal vesicles, and the levator ani muscle. There was no decrease in the number of mast cells, and no increase in the number of eosinophils.

THE SYNERGISTIC ACTION OF α-MELANOCYTE-STIMULATING HORMONE AND TESTOSTERONE ON THE SEBACEOUS, PROSTATE, PREPUTIAL, HARDERIAN AND LACHRYMAL GLANDS, SEMINAL VESICLES AND BROWN ADIPOSE TISSUE IN THE HYPOPHYSECTOMIZED-CASTRATED RAT

1975 ◽  
Vol 66 (3) ◽  
pp. 407-412 ◽  
Author(s):  
F. J. EBLING ◽  
ERIKA EBLING ◽  
VALERIE RANDALL ◽  
J. SKINNER
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Seminal Vesicles ◽  
Synergistic Action ◽  
The Other ◽  
Sebaceous Glands ◽  
Melanocyte Stimulating Hormone ◽  
Brown Adipose ◽  
Preputial Glands ◽  
Stimulating Hormone

SUMMARY α-Melanocyte-stimulating hormone was shown to act synergistically with testosterone to stimulate the sebaceous, prostate and preputial glands and the seminal vesicles in hypophysectomized-castrated rats. The sebaceous glands differed from the other three organs in that α-MSH not only acted synergistically, but also had a significant effect which was independent of the presence of exogenous testosterone. The response of the brown adipose tissue to testosterone, considerably reduced by hypophysectomy, was not restored by α-MSH. The Harderian and lachrymal glands were also pituitary-dependent and their weights in hypophysectomized-castrated rats were not restored by α-MSH.

EFFECTS OF TESTOSTERONE AND PROLACTIN OR GROWTH HORMONE ON THE ACCESSORY SEX ORGANS OF CASTRATED MICE

1975 ◽  
Vol 64 (1) ◽  
pp. 111-115 ◽  
Author(s):  
E. J. KEENAN ◽  
J. A. THOMAS
Keyword(s):  
Growth Hormone ◽  
Seminal Vesicle ◽  
Prostate Gland ◽  
Seminal Vesicles ◽  
Organ Weights ◽  
Simultaneous Injection ◽  
Androgenic Activity ◽  
Accessory Sex Organs ◽  
Anterior Prostate

SUMMARY In 5-day experiments, neither bovine prolactin (300 or 600 i.u./kg) nor ovine growth hormone (25 i.u./kg) alone significantly enhanced accessory sex organ weights in the castrated mouse. Seminal vesicle weights, and to a lesser extent anterior prostate gland weights, were augmented by the simultaneous injection of testosterone (1·5 mg/kg) daily plus prolactin or growth hormone. The effect was greater than that produced by testosterone alone. The levels of fructose in accessory sex organs used to indicate androgenic activity were similar in castrated mice receiving testosterone alone or in combination with prolactin or growth hormone. Prolactin alone did not influence uptake of [3H]testosterone by the seminal vesicles or anterior prostate gland over a 5 min period in vivo.

THE INHIBITION OF FERTILIZING ABILITY OF EPIDIDYMAL SPERMATOZOA BY THE ADMINISTRATION OF OESTRADIOL BENZOATE TO TESTOSTERONE-MAINTAINED HYPOPHYSECTOMIZED OR CASTRATED HAMSTERS

1974 ◽  
Vol 61 (1) ◽  
pp. 133-138 ◽  
Author(s):  
C. M. LUBICZ-NAWROCKI
Keyword(s):  
Adverse Effect ◽  
Seminal Vesicles ◽  
Daily Treatment ◽  
Ductus Deferens ◽  
Fertilizing Ability ◽  
Oestradiol Benzoate ◽  
Epididymal Spermatozoa ◽  
Cauda Epididymidis

SUMMARY Experiments were designed to discover if oestradiol benzoate induces loss of fertilizing ability of hamster epididymal spermatozoa by acting directly on the cauda epididymidis. Spermatozoa were retained in the cauda epididymidis by ligating the distal corpus epididymidis and proximal ductus deferens and fertilizing ability was compared in oestrogen-treated, intact hamsters and in castrated or hypophysectomized animals maintained with testosterone. Fertility tests showed that 12 daily s.c. injections of 3·5 or 5 μg oestradiol benzoate reduced fertilizing ability to 35·2 and 0%, respectively; supplementary testosterone did not prevent the adverse effect of oestrogen and reduced fertilizing ability was shown not to be related to a deficiency of circulating testosterone as judged by fructose concentration in the seminal vesicles. Daily treatment of androgen-maintained castrated or hypophysectomized hamsters with 3·5 μg oestradiol benzoate for 12 days reduced fertilizing ability to 43·7 and 31·3%, respectively. The results suggest that oestradiol benzoate reduces fertilizing ability in hamsters by acting directly on the cauda epididymidis and inhibiting the effect of testosterone.

TESTOSTERONE AND OESTRADIOL SUPPRESSION OF LH AND FSH IN ADULT MALE RATS: DURATION OF CASTRATION, DURATION OF TREATMENT AND COMBINED TREATMENT

1973 ◽  
Vol 73 (1) ◽  
pp. 11-21 ◽  
Author(s):  
R. S. Swerdloff ◽  
P. C. Walsh
Keyword(s):  
Adult Male ◽  
Testosterone Propionate ◽  
Combined Treatment ◽  
Male Rats ◽  
Low Doses ◽  
Duration Of Treatment ◽  
Serum Lh ◽  
Oestradiol Benzoate ◽  
Hypothalamic Pituitary Axis ◽  
Androgen Effect

ABSTRACT The effects of androgens and oestrogens on serum LH and FSH in castrated rats were evaluated with regard to the modifying influences of duration of castration, duration of treatment and combined oestrogen-androgen effect. Serum LH was not greatly influenced by these variables. In contrast, serum FSH was shown to be more resistant to suppression by both steroids after at least five days of castration, requiring a longer duration of treatment to be suppressed to intact levels. Combined treatment of submaximally suppressive doses of testosterone propionate and oestradiol benzoate resulted in no additive effect on lowering serum FSH. Low doses of both androgens and oestrogens resulted in elevated levels of serum LH and FSH, suggesting that the adult male hypothalamic-pituitary axis may be responsive to positive feedback. In all studies, testosterone preferentially suppressed serum LH as compared to serum FSH. In contrast, oestradiol administration produced parallel inhibition of both LH and FSH. It is emphasized that neither oestrogen nor androgen alone, nor in combination, resulted in preferential inhibition of serum FSH over LH.

ADRENAL EFFECT ON THE GROWTH OF THE VENTRAL AND DORSOLATERAL PROSTATE IN CASTRATED RATS INJECTED WITH OESTRADIOL

1972 ◽  
Vol 71 (1) ◽  
pp. 191-204 ◽  
Author(s):  
Lars-Eric Tisell
Keyword(s):  
Epithelial Cells ◽  
Secretory Activity ◽  
Reproductive Organs ◽  
Seminal Vesicles ◽  
Adrenal Steroids ◽  
Oestradiol Benzoate ◽  
Combined Actions ◽  
Adrenalectomized Rats ◽  
Epithelial Growth ◽  
Stimulation Of

ABSTRACT The growth of the ventral and dorsolateral prostate, the coagulating glands and the seminal vesicles was studied morphologically in castrated non-adrenalectomized and castrated adrenalectomized rats following injections for ten days of oestradiol benzoate. Oestradiol benzoate was given in daily doses of 0.001 mg or 0.010 mg, and to non-adrenalectomized rats also in daily doses of 0.100 mg. Oestradiol increased the weight of all the accessory reproductive organs in both the adrenalectomized and non-adrenalectomized rats. The weight increase of the dorsolateral prostate was most pronounced in the nonadrenalectomized rats. Oestradiol induced signs of secretory activity in epithelial cells of the ventral and dorsolateral prostate in the non-adrenalectomized but not in the adrenalectomized rats. The higher the dose of oestradiol the more marked was the stimulation of the epithelial growth in the ventral and dorsolateral prostate of the non-adrenalectomized rats. The growth and secretory activity observed in epithelial cells of the ventral and dorsolateral prostate of the oestradiol treated castrated non-adrenalectomized rats is assumed to be the result of the combined actions of oestradiol and adrenal steroids rather than merely the effect of an increased secretion of adrenal steroids induced by the oestradiol treatment.

SEXUAL BEHAVIOUR IN CASTRATED RABBITS TREATED WITH TESTOSTERONE, OESTRADIOL, DIHYDROTESTOSTERONE OR OESTRADIOL IN COMBINATION WITH DIHYDROTESTOSTERONE

1975 ◽  
Vol 67 (3) ◽  
pp. 327-332 ◽  
Author(s):  
A. ÅGMO ◽  
P. SÖDERSTEN
Keyword(s):  
Sexual Behaviour ◽  
Secretory Activity ◽  
Seminal Vesicles ◽  
The Other ◽  
Oestradiol Benzoate ◽  
Sexual Glands ◽  
The Brain

SUMMARY Sexual behaviour and the function of the accessory sexual glands were studied in castrated rabbits injected with testosterone benzoate (TB), oestradiol benzoate (OEB), dihydrotestosterone benzoate (DHTB) or OEB in combination with DHTB. Testosterone benzoate (1 mg daily for 90 days) stimulated the sexual behaviour more than any of the other steroids. The combination of OEB (0·33 mg) and DHTB (1 mg) was no more effective than either of these steroids given alone. The function of the accessory sexual glands was stimulated to a level comparable to that of intact animals given TB. Dihydrotestosterone benzoate was, however, not very effective in this respect. Oestradiol benzoate alone or in combination with DHTB caused hypertrophy and very low secretory activity of the seminal vesicles. These results suggest that testosterone itself is active both in the brain and in the accessory sexual glands in rabbits. This is in contrast to the rat, in which aromatization to oestradiol in the brain and reduction to DHT in the periphery seems to be important.

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